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The map label for this gene is mltD [H]

Identifier: 78048437

GI number: 78048437

Start: 3274364

End: 3275947

Strand: Reverse

Name: mltD [H]

Synonym: XCV2881

Alternate gene names: 78048437

Gene position: 3275947-3274364 (Counterclockwise)

Preceding gene: 78048439

Following gene: 78048434

Centisome position: 63.26

GC content: 66.04

Gene sequence:

>1584_bases
ATGCCGCTCGCTCATTTTGGTTTGCGCCCATGGCCTGTCCTGGCTGTGGTGGCGCTGATGTCTGTCGTCCCCGCCGCCCA
CGCCATCTCCAAACGCGACACGGAGAAGGCCGCCGCGCTCGATGCGCGCATGGCCGCCGCCGAAAAGCGCTATCGCGATG
CGCTGGTGCTGGTGAACAACTCCGACCCCAAGGGCACTGCCGAAGGCGATGCTGCGCTGGAGGACATGGAAGACATCATC
GACGCCTGCATCAAGCAGCGTGGTTGCCTGGTGTCCAATCAGTTGGCCACCTACAAGCGCCTGCTCAAGGCGCGCGCCGA
TGCCGAGGCGCCGGCCGCCGAAGATCCGGAAGACGACGACACGCTGCTGCAAGCCGACCCGGACCATCTGGGTCCGGCGT
CGAACGGCGTGCCCGAGGCCGCGCGTGCCGCCACGCTGCTCAACGACCAGCGCCACGAATTCGACAAGATGGTGCAGTAC
AACCCGGCCGTGCAGGCCGGTATCCGGCGCTGGCTCACCGATATGCGCCCGGCGCTGCTGACCAGCTACGAGAACTACAG
CAACCTGCGCGGCGTGATGTGGCCGGAATGGCAGAAGCGCGGCCTTCCAGAAGCGCTGCTGTTCGGCATCATGGCCAAGG
AATCCAACGGCAAGGTGCATGCCAGCTCGCGCGCTGGTGCCGCCGGGTTGATGCAGTTCATGCCCGCCACCGGACGTCGC
TTCGGGCTGGGCCCGGACGGCACCGGCTTCGACACCCGCTTCGATGCACGCAGCGCCGCCGAAGCCAGCGCCGCCTACCT
CAACGAACGCATGGCCGGACTCAACCGCAGCATCGAACTGGCACTGGCCGCCTACAACGGTGGCGAAGGCCGTGCCGCGC
GCGTGTTCGCGCAGAGCGGCGGGCGCGGCTTCTGGGACCAGGACGTCTATAACCAGTTCCCGGCCGAAACCAAGGACTAC
GTGCCAATGGTGATCGCGGCGGCGTGGATCTTCCTGCACCCGCGTCAATACGGGGTGGACTTTCCCAAGCTCGATGCGCA
GCCGGCCACGCTCAAGCTGGCCAAGTCGACCACCATCTACGAACTCACCATCTGCCTAGGCAGCATGGGCACACGCGACG
GCTACATGCGTGCGTTGCGCAATCTCAACCCACGTTACGAATCCGATGGCTGGATTCCGGCCGGCACGGTCATCAATGCC
ACCAACCGCATCGTCAACCTCTACACCCGTTACTGCGTCAATGGCCCGCGTGCGGATCTGGCGCGCACCCTGATCACCGC
CGATCTCAACAGCGCGATCGTGCGCAGCAGCAGCGCGCCCGAGTACGTGCCCAGCGTGGCGGTGGGCGATGTCAGCCAGA
TGGCCGGCGTCCCGACCACCGTCGCCACCGGTCAGCCGGCAGTGGCCAAGCCCAAGGCCAAGCAAGTGCGCAGCTACACC
ATCGCCAAGGGCGACACGCTCGGTCGCGTCGCGCAGAAGTACCAATGCGAGATCAAGGAGCTGGCCAAGGCCAACGGCTT
GAAAGCGCCGTCGTATGCGCTCAAGCCGGGGCAGAGCATCAGGTTGGCGGGGTGCGATCGCTGA

Upstream 100 bases:

>100_bases
CCCACCAAGCTAACGGCCCACTGACCCCGGTGGCCTCGCTGCCTTGCCCCGATGTTGCCCCGGCAGCTACCCTGCCTGCC
TTTCCGCTGTACTGCCTACG

Downstream 100 bases:

>100_bases
ACCGGGCGCTGGTGTGCGAGGCCTTGTGGGGAAGGCCTCGTCGCGGCCAGGTTCGCTGCTACGTTGGCAGCGAGCGCACG
ACAAGCCTGGTTGCTGTGGG

Product: lytic mureine transglycosylase precursor

Products: 1,6-Anhydrobond In The Muramic Acid Residue [C]

Alternate protein names: Murein hydrolase D; Regulatory protein dniR [H]

Number of amino acids: Translated: 527; Mature: 526

Protein sequence:

>527_residues
MPLAHFGLRPWPVLAVVALMSVVPAAHAISKRDTEKAAALDARMAAAEKRYRDALVLVNNSDPKGTAEGDAALEDMEDII
DACIKQRGCLVSNQLATYKRLLKARADAEAPAAEDPEDDDTLLQADPDHLGPASNGVPEAARAATLLNDQRHEFDKMVQY
NPAVQAGIRRWLTDMRPALLTSYENYSNLRGVMWPEWQKRGLPEALLFGIMAKESNGKVHASSRAGAAGLMQFMPATGRR
FGLGPDGTGFDTRFDARSAAEASAAYLNERMAGLNRSIELALAAYNGGEGRAARVFAQSGGRGFWDQDVYNQFPAETKDY
VPMVIAAAWIFLHPRQYGVDFPKLDAQPATLKLAKSTTIYELTICLGSMGTRDGYMRALRNLNPRYESDGWIPAGTVINA
TNRIVNLYTRYCVNGPRADLARTLITADLNSAIVRSSSAPEYVPSVAVGDVSQMAGVPTTVATGQPAVAKPKAKQVRSYT
IAKGDTLGRVAQKYQCEIKELAKANGLKAPSYALKPGQSIRLAGCDR

Sequences:

>Translated_527_residues
MPLAHFGLRPWPVLAVVALMSVVPAAHAISKRDTEKAAALDARMAAAEKRYRDALVLVNNSDPKGTAEGDAALEDMEDII
DACIKQRGCLVSNQLATYKRLLKARADAEAPAAEDPEDDDTLLQADPDHLGPASNGVPEAARAATLLNDQRHEFDKMVQY
NPAVQAGIRRWLTDMRPALLTSYENYSNLRGVMWPEWQKRGLPEALLFGIMAKESNGKVHASSRAGAAGLMQFMPATGRR
FGLGPDGTGFDTRFDARSAAEASAAYLNERMAGLNRSIELALAAYNGGEGRAARVFAQSGGRGFWDQDVYNQFPAETKDY
VPMVIAAAWIFLHPRQYGVDFPKLDAQPATLKLAKSTTIYELTICLGSMGTRDGYMRALRNLNPRYESDGWIPAGTVINA
TNRIVNLYTRYCVNGPRADLARTLITADLNSAIVRSSSAPEYVPSVAVGDVSQMAGVPTTVATGQPAVAKPKAKQVRSYT
IAKGDTLGRVAQKYQCEIKELAKANGLKAPSYALKPGQSIRLAGCDR
>Mature_526_residues
PLAHFGLRPWPVLAVVALMSVVPAAHAISKRDTEKAAALDARMAAAEKRYRDALVLVNNSDPKGTAEGDAALEDMEDIID
ACIKQRGCLVSNQLATYKRLLKARADAEAPAAEDPEDDDTLLQADPDHLGPASNGVPEAARAATLLNDQRHEFDKMVQYN
PAVQAGIRRWLTDMRPALLTSYENYSNLRGVMWPEWQKRGLPEALLFGIMAKESNGKVHASSRAGAAGLMQFMPATGRRF
GLGPDGTGFDTRFDARSAAEASAAYLNERMAGLNRSIELALAAYNGGEGRAARVFAQSGGRGFWDQDVYNQFPAETKDYV
PMVIAAAWIFLHPRQYGVDFPKLDAQPATLKLAKSTTIYELTICLGSMGTRDGYMRALRNLNPRYESDGWIPAGTVINAT
NRIVNLYTRYCVNGPRADLARTLITADLNSAIVRSSSAPEYVPSVAVGDVSQMAGVPTTVATGQPAVAKPKAKQVRSYTI
AKGDTLGRVAQKYQCEIKELAKANGLKAPSYALKPGQSIRLAGCDR

Specific function: Murein-degrading enzyme. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 LysM repeats [H]

Homologues:

Organism=Escherichia coli, GI1786405, Length=356, Percent_Identity=28.6516853932584, Blast_Score=90, Evalue=3e-19,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR010511
- InterPro:   IPR018392
- InterPro:   IPR002482
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01476 LysM; PF06474 MLTD_N; PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 57048; Mature: 56917

Theoretical pI: Translated: 8.84; Mature: 8.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLAHFGLRPWPVLAVVALMSVVPAAHAISKRDTEKAAALDARMAAAEKRYRDALVLVNN
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEC
SDPKGTAEGDAALEDMEDIIDACIKQRGCLVSNQLATYKRLLKARADAEAPAAEDPEDDD
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
TLLQADPDHLGPASNGVPEAARAATLLNDQRHEFDKMVQYNPAVQAGIRRWLTDMRPALL
CEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
TSYENYSNLRGVMWPEWQKRGLPEALLFGIMAKESNGKVHASSRAGAAGLMQFMPATGRR
HHHHHHHHCCCCCCCCHHHCCCCHHHHHHHHEECCCCCEEECCCCCHHHHHHHHCCCCCC
FGLGPDGTGFDTRFDARSAAEASAAYLNERMAGLNRSIELALAAYNGGEGRAARVFAQSG
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCEEEEEECCC
GRGFWDQDVYNQFPAETKDYVPMVIAAAWIFLHPRQYGVDFPKLDAQPATLKLAKSTTIY
CCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCEEEEECCCEEE
ELTICLGSMGTRDGYMRALRNLNPRYESDGWIPAGTVINATNRIVNLYTRYCVNGPRADL
EEEEHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHCCCCHHHH
ARTLITADLNSAIVRSSSAPEYVPSVAVGDVSQMAGVPTTVATGQPAVAKPKAKQVRSYT
HHHHHHHHHHHHHHCCCCCCCCCCCCHHCCHHHHCCCCCEECCCCCCCCCCHHHHHHHEE
IAKGDTLGRVAQKYQCEIKELAKANGLKAPSYALKPGQSIRLAGCDR
ECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCC
>Mature Secondary Structure 
PLAHFGLRPWPVLAVVALMSVVPAAHAISKRDTEKAAALDARMAAAEKRYRDALVLVNN
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEC
SDPKGTAEGDAALEDMEDIIDACIKQRGCLVSNQLATYKRLLKARADAEAPAAEDPEDDD
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
TLLQADPDHLGPASNGVPEAARAATLLNDQRHEFDKMVQYNPAVQAGIRRWLTDMRPALL
CEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
TSYENYSNLRGVMWPEWQKRGLPEALLFGIMAKESNGKVHASSRAGAAGLMQFMPATGRR
HHHHHHHHCCCCCCCCHHHCCCCHHHHHHHHEECCCCCEEECCCCCHHHHHHHHCCCCCC
FGLGPDGTGFDTRFDARSAAEASAAYLNERMAGLNRSIELALAAYNGGEGRAARVFAQSG
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCEEEEEECCC
GRGFWDQDVYNQFPAETKDYVPMVIAAAWIFLHPRQYGVDFPKLDAQPATLKLAKSTTIY
CCCCCCHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCEEEEECCCEEE
ELTICLGSMGTRDGYMRALRNLNPRYESDGWIPAGTVINATNRIVNLYTRYCVNGPRADL
EEEEHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHCCCCHHHH
ARTLITADLNSAIVRSSSAPEYVPSVAVGDVSQMAGVPTTVATGQPAVAKPKAKQVRSYT
HHHHHHHHHHHHHHCCCCCCCCCCCCHHCCHHHHCCCCCEECCCCCCCCCCHHHHHHHEE
IAKGDTLGRVAQKYQCEIKELAKANGLKAPSYALKPGQSIRLAGCDR
ECCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]