Click here to switch to the map view.

The map label for this gene is truA [H]

Identifier: 78048428

GI number: 78048428

Start: 3264343

End: 3265116

Strand: Reverse

Name: truA [H]

Synonym: XCV2872

Alternate gene names: 78048428

Gene position: 3265116-3264343 (Counterclockwise)

Preceding gene: 78048429

Following gene: 78048427

Centisome position: 63.05

GC content: 66.67

Gene sequence:

>774_bases
ATGCGTTACGCGCTGGGCGTGGAATACGACGGCAGCGAGTTCCAGGGCTGGCAGCAGTTGGGCGAGCATGGCGGGCCGAG
CGTGCAGGCCAGCCTGCAGGCGGCCTTGTCGTCGGTCGCCGATGCGCCGGTGCAGGTGGTCTGCGCCGGGCGCACCGACG
CGGGCGTGCATGGCGAATGCCAGGTGGTGCATTTCGATAGCGACGCACGCCGCGAGCCGCGTGGCTGGATGCTGGGCACC
ACTGCGCGCCTGCCGCCGTCGATCGCCGTGCGCTGGTGCGTGCCGGCAGCCGATGACTTCCACGCGCGCTTTTCCGCACG
CGCGCGTCGTTACCGCTATCGCCTGCTCAATCGGCAGATTCGCCCAGCCCTGTATCGGCAGACCCTGAGCTGGGAGCGTC
GCCCGCTCGATGCCGATGCCATGCATGCGGCGGCGCAGGCCTTATTGGGCGAGAACGATTTCAGCGCGTTCCGCAGCGTG
CAATGTCAGGCCTTGCACGCGCGCCGTCACCTGCAGGCCATCCACGTGCAGCGTATCGGCGAGGTGGTGGAGGTGCAGGT
CCAGGCCAATGCATTCCTTCATCACATGGTGCGGAATATTGTGGGTTCGCTGATTTTGGTCGGTACCGGCGAGCAGCCGG
CCGACTGGATCGCGACGTTGCTCGCCGGACGCGACCGCACTGTGGCCGGCCCGACAGCGCCGCCACAAGGTCTGGTATTT
ATCGGACCTCTGTATCCTGCAGAGTGGCATCTGCCTGCTGAGGTGACCCAATGA

Upstream 100 bases:

>100_bases
ACCTTTTTTGCAGCGACGGGGCTGTGCTGGCAATCGCCTGCGATGGCGGCGGCATGACCGGCGCGCCGATTTCATCGATT
CACTCATAGGGCAGGTACCG

Downstream 100 bases:

>100_bases
ATCGATCGCTGTACCGCACCCGTATCAAGTTTTGTGGCATGACCCGCGCCGGCGATATCCGGCTGGCCGGCGAGCTGGGT
GTGGACGCGGTAGGTTTCAT

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I [H]

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MRYALGVEYDGSEFQGWQQLGEHGGPSVQASLQAALSSVADAPVQVVCAGRTDAGVHGECQVVHFDSDARREPRGWMLGT
TARLPPSIAVRWCVPAADDFHARFSARARRYRYRLLNRQIRPALYRQTLSWERRPLDADAMHAAAQALLGENDFSAFRSV
QCQALHARRHLQAIHVQRIGEVVEVQVQANAFLHHMVRNIVGSLILVGTGEQPADWIATLLAGRDRTVAGPTAPPQGLVF
IGPLYPAEWHLPAEVTQ

Sequences:

>Translated_257_residues
MRYALGVEYDGSEFQGWQQLGEHGGPSVQASLQAALSSVADAPVQVVCAGRTDAGVHGECQVVHFDSDARREPRGWMLGT
TARLPPSIAVRWCVPAADDFHARFSARARRYRYRLLNRQIRPALYRQTLSWERRPLDADAMHAAAQALLGENDFSAFRSV
QCQALHARRHLQAIHVQRIGEVVEVQVQANAFLHHMVRNIVGSLILVGTGEQPADWIATLLAGRDRTVAGPTAPPQGLVF
IGPLYPAEWHLPAEVTQ
>Mature_257_residues
MRYALGVEYDGSEFQGWQQLGEHGGPSVQASLQAALSSVADAPVQVVCAGRTDAGVHGECQVVHFDSDARREPRGWMLGT
TARLPPSIAVRWCVPAADDFHARFSARARRYRYRLLNRQIRPALYRQTLSWERRPLDADAMHAAAQALLGENDFSAFRSV
QCQALHARRHLQAIHVQRIGEVVEVQVQANAFLHHMVRNIVGSLILVGTGEQPADWIATLLAGRDRTVAGPTAPPQGLVF
IGPLYPAEWHLPAEVTQ

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs [H]

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family [H]

Homologues:

Organism=Homo sapiens, GI23503259, Length=275, Percent_Identity=30.9090909090909, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI1788657, Length=251, Percent_Identity=54.5816733067729, Blast_Score=271, Evalue=3e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094 [H]

Pfam domain/function: PF01416 PseudoU_synth_1 [H]

EC number: =5.4.99.12 [H]

Molecular weight: Translated: 28363; Mature: 28363

Theoretical pI: Translated: 8.04; Mature: 8.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYALGVEYDGSEFQGWQQLGEHGGPSVQASLQAALSSVADAPVQVVCAGRTDAGVHGEC
CCEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEE
QVVHFDSDARREPRGWMLGTTARLPPSIAVRWCVPAADDFHARFSARARRYRYRLLNRQI
EEEEECCCCCCCCCCCEEECCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
RPALYRQTLSWERRPLDADAMHAAAQALLGENDFSAFRSVQCQALHARRHLQAIHVQRIG
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
EVVEVQVQANAFLHHMVRNIVGSLILVGTGEQPADWIATLLAGRDRTVAGPTAPPQGLVF
HHEEEEEHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCEEE
IGPLYPAEWHLPAEVTQ
EECCCCCCCCCCCCCCC
>Mature Secondary Structure
MRYALGVEYDGSEFQGWQQLGEHGGPSVQASLQAALSSVADAPVQVVCAGRTDAGVHGEC
CCEEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCEE
QVVHFDSDARREPRGWMLGTTARLPPSIAVRWCVPAADDFHARFSARARRYRYRLLNRQI
EEEEECCCCCCCCCCCEEECCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHH
RPALYRQTLSWERRPLDADAMHAAAQALLGENDFSAFRSVQCQALHARRHLQAIHVQRIG
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHC
EVVEVQVQANAFLHHMVRNIVGSLILVGTGEQPADWIATLLAGRDRTVAGPTAPPQGLVF
HHEEEEEHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCCCCEEE
IGPLYPAEWHLPAEVTQ
EECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]