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The map label for this gene is trpA [H]

Identifier: 78048421

GI number: 78048421

Start: 3258740

End: 3259546

Strand: Reverse

Name: trpA [H]

Synonym: XCV2865

Alternate gene names: 78048421

Gene position: 3259546-3258740 (Counterclockwise)

Preceding gene: 78048422

Following gene: 162138533

Centisome position: 62.94

GC content: 69.76

Gene sequence:

>807_bases
ATGCGTCGTATCGATTCCTGCTTCGCCGAGCTGCGTGCCAATGGGCGCAAGGCACTGATCCCCTTCATCACCGCCGGCGA
CCCATCGTTGGAAGCCACCGTGCCGGTGATGCACGCGCTGGTGCGCGCCGGTGCCGATGTGATCGAGCTGGGGGTGCCGT
TCTCCGACCCCATGGCCGACGGTCCCACCATCCAGCGCAGCTCCGAGCGCGCGCTGGGCCGCGGTGCCGGCCTGGCGTAT
GTGCTCGAAGCGGTGCACGAGTTCCGCCGCGAAGATGCCACCACCCCGGTGGTGTTGATGGGCTACCTCAACCCGATCGA
GATCCATGGCACGCGACGCTTCGCCGAAGCCGCGGTCGCCGCGGGCGTGGATGGTGTGCTGCTGGTCGACCTGCCGCCGG
AAGAAGCCGGCGAAACGCGCGCCATCTTCACCGAGGTCGGCCTGGCGCTGATCGCACTGGCCTCGCCCACCACCAGCGAG
CAGCGCCTGGACATGTTGTGCAGCACCGCGCAGGGCTATCTGTACTACGTCAGCTTTGCCGGCGTCACCGGCGCCTCCAA
TCTGCTCGACACCCATGCCGCCAGCGATCGCCTGCGCCAGCTGCGCCAGCGTGCCGGCGCGCCGGTGGTGGCCGGCTTCG
GCATCAAGGATGCGGCCAGCGCCGCTGCCATGGCCGTGGATGCCGACGGCGTGGTGGTGGGCAGTGCCCTGGTCGCCGCA
TTGGCGGACGCAGGCGAGGTCCGCAGCGCCCGTGAGCGCGCCGAAGCGTTCCTGGCCCCGTTGCGCCAGGCGCTCGACCA
GAAATGA

Upstream 100 bases:

>100_bases
CACGGCTACCTGTTTGCGCTGTGCTTTGCGCTGATGCGGTATTTCTTTGCCACCTGAGGTTAGATGGCAGCTGGCCTTCC
TCCCTTGTTCGAGTGATTTC

Downstream 100 bases:

>100_bases
GAACCGAACGTCTCTCTCAGCGCTAACGTCTCTCTCAGCGCTAGGGAGTGGGGCGAGGGGGCGGGCGCAGCCACGTGCAG
CCAATCCATTGCGCCGCATC

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MRRIDSCFAELRANGRKALIPFITAGDPSLEATVPVMHALVRAGADVIELGVPFSDPMADGPTIQRSSERALGRGAGLAY
VLEAVHEFRREDATTPVVLMGYLNPIEIHGTRRFAEAAVAAGVDGVLLVDLPPEEAGETRAIFTEVGLALIALASPTTSE
QRLDMLCSTAQGYLYYVSFAGVTGASNLLDTHAASDRLRQLRQRAGAPVVAGFGIKDAASAAAMAVDADGVVVGSALVAA
LADAGEVRSARERAEAFLAPLRQALDQK

Sequences:

>Translated_268_residues
MRRIDSCFAELRANGRKALIPFITAGDPSLEATVPVMHALVRAGADVIELGVPFSDPMADGPTIQRSSERALGRGAGLAY
VLEAVHEFRREDATTPVVLMGYLNPIEIHGTRRFAEAAVAAGVDGVLLVDLPPEEAGETRAIFTEVGLALIALASPTTSE
QRLDMLCSTAQGYLYYVSFAGVTGASNLLDTHAASDRLRQLRQRAGAPVVAGFGIKDAASAAAMAVDADGVVVGSALVAA
LADAGEVRSARERAEAFLAPLRQALDQK
>Mature_268_residues
MRRIDSCFAELRANGRKALIPFITAGDPSLEATVPVMHALVRAGADVIELGVPFSDPMADGPTIQRSSERALGRGAGLAY
VLEAVHEFRREDATTPVVLMGYLNPIEIHGTRRFAEAAVAAGVDGVLLVDLPPEEAGETRAIFTEVGLALIALASPTTSE
QRLDMLCSTAQGYLYYVSFAGVTGASNLLDTHAASDRLRQLRQRAGAPVVAGFGIKDAASAAAMAVDADGVVVGSALVAA
LADAGEVRSARERAEAFLAPLRQALDQK

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate [H]

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family [H]

Homologues:

Organism=Escherichia coli, GI1787514, Length=218, Percent_Identity=37.6146788990826, Blast_Score=146, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6321412, Length=262, Percent_Identity=39.6946564885496, Blast_Score=194, Evalue=2e-50,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028 [H]

Pfam domain/function: PF00290 Trp_syntA [H]

EC number: =4.2.1.20 [H]

Molecular weight: Translated: 28067; Mature: 28067

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRIDSCFAELRANGRKALIPFITAGDPSLEATVPVMHALVRAGADVIELGVPFSDPMAD
CCHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCC
GPTIQRSSERALGRGAGLAYVLEAVHEFRREDATTPVVLMGYLNPIEIHGTRRFAEAAVA
CCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCEEEECCHHHHHHHHHH
AGVDGVLLVDLPPEEAGETRAIFTEVGLALIALASPTTSEQRLDMLCSTAQGYLYYVSFA
CCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCEEEEEEEC
GVTGASNLLDTHAASDRLRQLRQRAGAPVVAGFGIKDAASAAAMAVDADGVVVGSALVAA
CCCCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHEEECCCCCHHHHHHHHH
LADAGEVRSARERAEAFLAPLRQALDQK
HHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRRIDSCFAELRANGRKALIPFITAGDPSLEATVPVMHALVRAGADVIELGVPFSDPMAD
CCHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCC
GPTIQRSSERALGRGAGLAYVLEAVHEFRREDATTPVVLMGYLNPIEIHGTRRFAEAAVA
CCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHCCCCCEEEEECCCEEEECCHHHHHHHHHH
AGVDGVLLVDLPPEEAGETRAIFTEVGLALIALASPTTSEQRLDMLCSTAQGYLYYVSFA
CCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCEEEEEEEC
GVTGASNLLDTHAASDRLRQLRQRAGAPVVAGFGIKDAASAAAMAVDADGVVVGSALVAA
CCCCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCCHHHHHHHHEEECCCCCHHHHHHHHH
LADAGEVRSARERAEAFLAPLRQALDQK
HHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]