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The map label for this gene is purK [H]

Identifier: 78048384

GI number: 78048384

Start: 3217825

End: 3218970

Strand: Reverse

Name: purK [H]

Synonym: XCV2828

Alternate gene names: 78048384

Gene position: 3218970-3217825 (Counterclockwise)

Preceding gene: 78048385

Following gene: 78048382

Centisome position: 62.16

GC content: 67.1

Gene sequence:

>1146_bases
ATGAGCACGGTTGGCATTCTCGGTGGCGGCCAGCTGGCGCGCATGCTGGTACTGGCCGGTGCGCCGCTCGGCATACGCTT
TGCGGTCTTCGATCCGGCAGTCGATGCCTGCGCAGGCCAGGTGGCACCGCTGCAGGTTGGCGCGTTCGATGACGTGGCGA
CCCTTGCCGCATTCGCCGGGCAGGTGGACGTGATCACCTTCGATTTCGAAAACGTGCCGGCCGCCAGCGCGCAGCAACTT
GCCGCGCAGGTGCCGGTGTTTCCGAGTCCGGCGGCGCTTGCGGTTGCGCAGGACCGGCTCAGCGAAAAAACCTTGTTCCG
CGAACTCGGCATTCCGGTGCCCGAGTTTGCCGCGATCGACGACCGCGTCGGCCTGGACGCAGCGCTCGCGCGGATCGGTA
CGCCCTGCGTGCTGAAGACGCGCCGCTTCGGCTACGACGGCAAGGGCCAGTTCCGGATCAAGACGCTCGCCGATGCCGAT
GCAGCGTGGGACGCGCTTGGCGCCCAGGCGGCGCACGTCGGGTTGATCGTGGAAGCGTTCGTGCCGTTCCAGCGCGAGGT
CAGCGTGGTTGCAGTACGCGGCCGCGATGGCGAATTCCGCGCCTGGCCGTTGACCGAAAACTGGCATGTGGACGGTGTGC
TGTCGGCCAGCCTGGCACCGGCGAGCGTGGAGGCGGCCCTTCAAGCCTCGGCCGAATCGCACGCGCGTGCCGTGGCGGAA
AAGCTCGACTATGTCGGTGTATTTGCGCTGGAACTGTTCGTGCGCGACGGTGAGTTGCTGGCCAACGAAATGGCGCCGCG
CGTGCATAACTCGGGGCACTGGACCATCGAAGGTGCCGAGACCTCGCAATTCGAAAATCATGTGCGGGCCGTCCTTGGCT
TGCCACTGGGCAGTACTGCGATGCGCGGCTACGCCTGCATGCTCAACTGGCTGGGCGCGATGCCAGAGGCCAGGGCGTTT
CTGGCGGTGCCCGGTGGGCATTGGCACGACTACGGCAAGCAAGCGCGCGATGGACGCAAGGTGGGGCACGCAACGCTGCG
CGCGGAGACAGCGCAGGCGCTGGCACTGGCGGTGAGCGATGCCGGCGCGGCGTTGCAGCGCGATGCGCAAGCTCGGCCGG
TGGTTGCGCGTTTGAACGAAGGGTGA

Upstream 100 bases:

>100_bases
CGGCCATGCTGGCGCCCGAGCAGGCGCAGGTCGGTCAGGCGCTGGCGCAGTTCCGCGCCAAGCAGACCGACGATGTCATG
GCCAGCGACGACCCGCGCCA

Downstream 100 bases:

>100_bases
GGCGCGTAGCGTTTCGGTTGCGATCACGCGCGACGACACCTCCGTTTGCGCGGGCGCGTCGGTGTCGGACGCGGACCCGT
TCTGCGATGTGTCAGCAGAT

Product: phosphoribosylaminoimidazole carboxylase ATPase subunit

Products: NA

Alternate protein names: N5-CAIR synthase; 5-(carboxyamino)imidazole ribonucleotide synthetase [H]

Number of amino acids: Translated: 381; Mature: 380

Protein sequence:

>381_residues
MSTVGILGGGQLARMLVLAGAPLGIRFAVFDPAVDACAGQVAPLQVGAFDDVATLAAFAGQVDVITFDFENVPAASAQQL
AAQVPVFPSPAALAVAQDRLSEKTLFRELGIPVPEFAAIDDRVGLDAALARIGTPCVLKTRRFGYDGKGQFRIKTLADAD
AAWDALGAQAAHVGLIVEAFVPFQREVSVVAVRGRDGEFRAWPLTENWHVDGVLSASLAPASVEAALQASAESHARAVAE
KLDYVGVFALELFVRDGELLANEMAPRVHNSGHWTIEGAETSQFENHVRAVLGLPLGSTAMRGYACMLNWLGAMPEARAF
LAVPGGHWHDYGKQARDGRKVGHATLRAETAQALALAVSDAGAALQRDAQARPVVARLNEG

Sequences:

>Translated_381_residues
MSTVGILGGGQLARMLVLAGAPLGIRFAVFDPAVDACAGQVAPLQVGAFDDVATLAAFAGQVDVITFDFENVPAASAQQL
AAQVPVFPSPAALAVAQDRLSEKTLFRELGIPVPEFAAIDDRVGLDAALARIGTPCVLKTRRFGYDGKGQFRIKTLADAD
AAWDALGAQAAHVGLIVEAFVPFQREVSVVAVRGRDGEFRAWPLTENWHVDGVLSASLAPASVEAALQASAESHARAVAE
KLDYVGVFALELFVRDGELLANEMAPRVHNSGHWTIEGAETSQFENHVRAVLGLPLGSTAMRGYACMLNWLGAMPEARAF
LAVPGGHWHDYGKQARDGRKVGHATLRAETAQALALAVSDAGAALQRDAQARPVVARLNEG
>Mature_380_residues
STVGILGGGQLARMLVLAGAPLGIRFAVFDPAVDACAGQVAPLQVGAFDDVATLAAFAGQVDVITFDFENVPAASAQQLA
AQVPVFPSPAALAVAQDRLSEKTLFRELGIPVPEFAAIDDRVGLDAALARIGTPCVLKTRRFGYDGKGQFRIKTLADADA
AWDALGAQAAHVGLIVEAFVPFQREVSVVAVRGRDGEFRAWPLTENWHVDGVLSASLAPASVEAALQASAESHARAVAEK
LDYVGVFALELFVRDGELLANEMAPRVHNSGHWTIEGAETSQFENHVRAVLGLPLGSTAMRGYACMLNWLGAMPEARAFL
AVPGGHWHDYGKQARDGRKVGHATLRAETAQALALAVSDAGAALQRDAQARPVVARLNEG

Specific function: Catalyzes the ATP-dependent conversion of 5- aminoimidazole ribonucleotide (AIR) and HCO(3)(-) to N5- carboxyaminoimidazole ribonucleotide (N5-CAIR) [H]

COG id: COG0026

COG function: function code F; Phosphoribosylaminoimidazole carboxylase (NCAIR synthetase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786733, Length=346, Percent_Identity=38.728323699422, Blast_Score=201, Evalue=4e-53,
Organism=Escherichia coli, GI1788155, Length=311, Percent_Identity=28.6173633440514, Blast_Score=93, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6324702, Length=374, Percent_Identity=33.6898395721925, Blast_Score=201, Evalue=2e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005875
- InterPro:   IPR011761
- InterPro:   IPR003135
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR011054 [H]

Pfam domain/function: PF02222 ATP-grasp [H]

EC number: =6.3.4.18 [H]

Molecular weight: Translated: 40143; Mature: 40012

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: PS50975 ATP_GRASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTVGILGGGQLARMLVLAGAPLGIRFAVFDPAVDACAGQVAPLQVGAFDDVATLAAFAG
CCCEEECCCHHHHHHHHHCCCCCCEEEEEECCHHHHHCCCCCEEEECCCHHHHHHHHHCC
QVDVITFDFENVPAASAQQLAAQVPVFPSPAALAVAQDRLSEKTLFRELGIPVPEFAAID
CEEEEEEECCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHCCC
DRVGLDAALARIGTPCVLKTRRFGYDGKGQFRIKTLADADAAWDALGAQAAHVGLIVEAF
CCCCHHHHHHHHCCCEEEEHHHCCCCCCCCEEEEEECCCHHHHHHHCCHHHHHHHHHHHH
VPFQREVSVVAVRGRDGEFRAWPLTENWHVDGVLSASLAPASVEAALQASAESHARAVAE
CCCCCCEEEEEEECCCCCEEEECCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHH
KLDYVGVFALELFVRDGELLANEMAPRVHNSGHWTIEGAETSQFENHVRAVLGLPLGSTA
HHHHHHHEEHHHHHCCCHHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHHHHCCCCCHHH
MRGYACMLNWLGAMPEARAFLAVPGGHWHDYGKQARDGRKVGHATLRAETAQALALAVSD
HHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHEEEECC
AGAALQRDAQARPVVARLNEG
CCHHHHCCCCCCCEEEECCCC
>Mature Secondary Structure 
STVGILGGGQLARMLVLAGAPLGIRFAVFDPAVDACAGQVAPLQVGAFDDVATLAAFAG
CCEEECCCHHHHHHHHHCCCCCCEEEEEECCHHHHHCCCCCEEEECCCHHHHHHHHHCC
QVDVITFDFENVPAASAQQLAAQVPVFPSPAALAVAQDRLSEKTLFRELGIPVPEFAAID
CEEEEEEECCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCHHCCC
DRVGLDAALARIGTPCVLKTRRFGYDGKGQFRIKTLADADAAWDALGAQAAHVGLIVEAF
CCCCHHHHHHHHCCCEEEEHHHCCCCCCCCEEEEEECCCHHHHHHHCCHHHHHHHHHHHH
VPFQREVSVVAVRGRDGEFRAWPLTENWHVDGVLSASLAPASVEAALQASAESHARAVAE
CCCCCCEEEEEEECCCCCEEEECCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHH
KLDYVGVFALELFVRDGELLANEMAPRVHNSGHWTIEGAETSQFENHVRAVLGLPLGSTA
HHHHHHHEEHHHHHCCCHHHHHHHCCCCCCCCCEEEECCCHHHHHHHHHHHHCCCCCHHH
MRGYACMLNWLGAMPEARAFLAVPGGHWHDYGKQARDGRKVGHATLRAETAQALALAVSD
HHHHHHHHHHHCCCCCCCEEEECCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHEEEECC
AGAALQRDAQARPVVARLNEG
CCHHHHCCCCCCCEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8816818; 10984043 [H]