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The map label for this gene is virB8 [H]

Identifier: 78048364

GI number: 78048364

Start: 3194231

End: 3195247

Strand: Reverse

Name: virB8 [H]

Synonym: XCV2808

Alternate gene names: 78048364

Gene position: 3195247-3194231 (Counterclockwise)

Preceding gene: 78048365

Following gene: 78048363

Centisome position: 61.7

GC content: 53.39

Gene sequence:

>1017_bases
ATGTTGCGTAAGAAGGTCAGTGAAAAGGACGGTTCCGCCCAAGTAGGTGCGGCCGTTCAAAAAGCGGTGAATTACGAGGT
CAGCATTGCGGACCTCGCGCGCCGCAGCGAAAAGCGTGCCTGGATCGTGGCGACGCTGTCGATGCTGGTCACCGTCATGA
CAGCGGGCGGCTACTACTACATGCTGCCGTTGAAGGAGAAAGTGCCATATCTGGTCATGGCGGACGCGTATTCCGGTACC
AGCACCATTGCGAAGCTTGAAGCGAATTTTGGTGGACGCATGGTCAGCACTAGCGAGGCATTAGCGCGCAGCAATGTTGC
ACGTTTCATTACATCGCGTGAATCGTTTGATGCCTCGACGATAGGTGACCGTGATTGGAACACTGTCGTTGCCATGGCTA
CAGGTAACGTTCTTGGTGAATATCGTGCGCTACATGCTGGAAATAATCCCGCCAGACCTTTTAATACTTACGGCACCTCT
CGTGCTATTCGGGTCAATATTCTCAGTATTACGCTTATAGGAGGAAGTGGTAAGCCTTACACTGGTGCCACTGTACGTTT
CCAACGAAATGTGTATGACAAAGCCTCGGCCGCGGCCACGTTCTTAGATAACAAGATCGCTACAATGGGTTTTGTCTATC
AAGATAATCTTGAGATGAGTGATACCCTTCGGGTGGAAAATCCACTGGGATTTCGTGTCACAGATTATCGTGTGGATAAT
GATTATTCGTCTTTACCGGCAGCGCCCTCTTCGGTTAGCCCGCAAGCCCAAGTTGCGCCACAGATTGCAGCTCCTCAGCA
GGCCGCATTAGGCGTAGTAGATCCAAACGCGGCCGCCGCAGCCGGGCAGGGTAGTGTTTCAGCCCAGCAGGGTGTTCTTA
ATCAACAGGGGATGCCTGTCCAGCAGGTGCCGCCAGGATATTCCAGCCAAGTGCAGCCGGCGAGCCAACCTCAGGCGCAG
CCCGCGGTCCAATATCAAGGGATGCCGAACAATGTCAAAGGAGCTAGCGGTCGATGA

Upstream 100 bases:

>100_bases
CAGGCATGGGGCCGGCGGTGTTCCTACCTTCTCAAGTACCCGCGACGCGTTGCGGCGATGGTTTCAGGCCTGACAAGGCC
TCACGGTGACGGATCTAGAC

Downstream 100 bases:

>100_bases
AACCCTCTAAAATCTATGGGGCTGTATTGACTGCAGCGCTAATATTTCCCTTATGTGTGAGTTCGGTGAACGCTCAGGTC
GTGCAGGAGTATGAGTACGC

Product: type IV secretion system protein VirB8

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MLRKKVSEKDGSAQVGAAVQKAVNYEVSIADLARRSEKRAWIVATLSMLVTVMTAGGYYYMLPLKEKVPYLVMADAYSGT
STIAKLEANFGGRMVSTSEALARSNVARFITSRESFDASTIGDRDWNTVVAMATGNVLGEYRALHAGNNPARPFNTYGTS
RAIRVNILSITLIGGSGKPYTGATVRFQRNVYDKASAAATFLDNKIATMGFVYQDNLEMSDTLRVENPLGFRVTDYRVDN
DYSSLPAAPSSVSPQAQVAPQIAAPQQAALGVVDPNAAAAAGQGSVSAQQGVLNQQGMPVQQVPPGYSSQVQPASQPQAQ
PAVQYQGMPNNVKGASGR

Sequences:

>Translated_338_residues
MLRKKVSEKDGSAQVGAAVQKAVNYEVSIADLARRSEKRAWIVATLSMLVTVMTAGGYYYMLPLKEKVPYLVMADAYSGT
STIAKLEANFGGRMVSTSEALARSNVARFITSRESFDASTIGDRDWNTVVAMATGNVLGEYRALHAGNNPARPFNTYGTS
RAIRVNILSITLIGGSGKPYTGATVRFQRNVYDKASAAATFLDNKIATMGFVYQDNLEMSDTLRVENPLGFRVTDYRVDN
DYSSLPAAPSSVSPQAQVAPQIAAPQQAALGVVDPNAAAAAGQGSVSAQQGVLNQQGMPVQQVPPGYSSQVQPASQPQAQ
PAVQYQGMPNNVKGASGR
>Mature_338_residues
MLRKKVSEKDGSAQVGAAVQKAVNYEVSIADLARRSEKRAWIVATLSMLVTVMTAGGYYYMLPLKEKVPYLVMADAYSGT
STIAKLEANFGGRMVSTSEALARSNVARFITSRESFDASTIGDRDWNTVVAMATGNVLGEYRALHAGNNPARPFNTYGTS
RAIRVNILSITLIGGSGKPYTGATVRFQRNVYDKASAAATFLDNKIATMGFVYQDNLEMSDTLRVENPLGFRVTDYRVDN
DYSSLPAAPSSVSPQAQVAPQIAAPQQAALGVVDPNAAAAAGQGSVSAQQGVLNQQGMPVQQVPPGYSSQVQPASQPQAQ
PAVQYQGMPNNVKGASGR

Specific function: The virB operon is essential for intracellular survival and is not involved in the invasion process. Constitutes a major determinant of virulence in mice [H]

COG id: COG3736

COG function: function code U; Type IV secretory pathway, component VirB8

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the virB8 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007430 [H]

Pfam domain/function: PF04335 VirB8 [H]

EC number: NA

Molecular weight: Translated: 35957; Mature: 35957

Theoretical pI: Translated: 9.79; Mature: 9.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRKKVSEKDGSAQVGAAVQKAVNYEVSIADLARRSEKRAWIVATLSMLVTVMTAGGYYY
CCCCCCCCCCCCHHHHHHHHHHHCCEEEHHHHHHHCCCCEEHHHHHHHHHHHHHCCCEEE
MLPLKEKVPYLVMADAYSGTSTIAKLEANFGGRMVSTSEALARSNVARFITSRESFDAST
EEEHHHCCCEEEEEECCCCCCEEEEEECCCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCC
IGDRDWNTVVAMATGNVLGEYRALHAGNNPARPFNTYGTSRAIRVNILSITLIGGSGKPY
CCCCCCCEEEEEECCCHHHHHHHEECCCCCCCCCCCCCCCCEEEEEEEEEEEECCCCCCC
TGATVRFQRNVYDKASAAATFLDNKIATMGFVYQDNLEMSDTLRVENPLGFRVTDYRVDN
CCEEEEEECCHHHHHHHHHHHHCCCHHHEEEEEECCCCCCCEEEECCCCCEEEEEEEECC
DYSSLPAAPSSVSPQAQVAPQIAAPQQAALGVVDPNAAAAAGQGSVSAQQGVLNQQGMPV
CCCCCCCCCCCCCCHHHCCCCCCCCCHHEEEEECCCCCCCCCCCCCHHHHCCCCCCCCCH
QQVPPGYSSQVQPASQPQAQPAVQYQGMPNNVKGASGR
HCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCC
>Mature Secondary Structure
MLRKKVSEKDGSAQVGAAVQKAVNYEVSIADLARRSEKRAWIVATLSMLVTVMTAGGYYY
CCCCCCCCCCCCHHHHHHHHHHHCCEEEHHHHHHHCCCCEEHHHHHHHHHHHHHCCCEEE
MLPLKEKVPYLVMADAYSGTSTIAKLEANFGGRMVSTSEALARSNVARFITSRESFDAST
EEEHHHCCCEEEEEECCCCCCEEEEEECCCCCEEEEHHHHHHHHHHHHHHHCCCCCCCCC
IGDRDWNTVVAMATGNVLGEYRALHAGNNPARPFNTYGTSRAIRVNILSITLIGGSGKPY
CCCCCCCEEEEEECCCHHHHHHHEECCCCCCCCCCCCCCCCEEEEEEEEEEEECCCCCCC
TGATVRFQRNVYDKASAAATFLDNKIATMGFVYQDNLEMSDTLRVENPLGFRVTDYRVDN
CCEEEEEECCHHHHHHHHHHHHCCCHHHEEEEEECCCCCCCEEEECCCCCEEEEEEEECC
DYSSLPAAPSSVSPQAQVAPQIAAPQQAALGVVDPNAAAAAGQGSVSAQQGVLNQQGMPV
CCCCCCCCCCCCCCHHHCCCCCCCCCHHEEEEECCCCCCCCCCCCCHHHHCCCCCCCCCH
QQVPPGYSSQVQPASQPQAQPAVQYQGMPNNVKGASGR
HCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10940027 [H]