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The map label for this gene is puuD [H]

Identifier: 78048213

GI number: 78048213

Start: 2994926

End: 2995681

Strand: Reverse

Name: puuD [H]

Synonym: XCV2657

Alternate gene names: 78048213

Gene position: 2995681-2994926 (Counterclockwise)

Preceding gene: 78048218

Following gene: 78048212

Centisome position: 57.85

GC content: 67.99

Gene sequence:

>756_bases
ATGGCAGTCGTTCCGCTGGTCGGCCTGCCGACCGATCGCATCCTGCAAGGGCCTCACCCGTTTCTGGCTGCCGGCGAGAA
GTACATCCGCGCGGTGATCGAGGCAGCCGCCGCGATGCCGGTGCTGTTGCCCAGCCTGCAGCCGGCATTGGATGCAGGCG
CGTGGCTGGAGCGGCTGGATGGGCTGTTGTTGACCGGGGCGGTCAGCAATGTGGAGCCGCATCACTACAGTGACGAGCCC
AGCTGGGAAGGCAACCCGCACGACCCGGCGCGCGATGCCACCATCCTTGCGCTCATCCCGCAGGCGCTTGCGCGTGGCCT
GCCGGTGCTGGCGATCTGCCGCGGGCTGCAGGAGGTCAATGTGGCACTGGGCGGGCGCCTGCATCAGCGCGTGCACGACG
TGCCCGGCCTGGCCGACCACCGGGAAGACCGCGGCACACCGATAGACATGCAATATGGCCCGGCGCATGTGGTGCACCTG
CATCCCGGCGGCTGGCTGGCGGGCATGAGCGATAGCGCCCAGGTCTGGGTCAATTCCCTGCACGGGCAGGGCATTGCCAC
GCTGGCTGAGGGGTTGTTGATCGAAGCCACCGCGCCGGACGGGCTGATCGAAGCGTTCCGCGGCTCCGGCCCGGGGTTTC
TGCTGGCAGTGCAGTGGCATCCGGAATGGCGTGTCACACAGCATCCGTTCTACCGTGCCATCTTTCAGGCCTTCGGCGAG
GCCTGTCGCCAATACGCCGCGCGACGCGGCAAGTGA

Upstream 100 bases:

>100_bases
GTGAGTAATCGCAAGCGCAGTGGCACGCTTGGCAATGCCGGCAATGGCGCGGTAACTTCGAGCGTATCGACGTTCCACCG
AACGTATCGGATTTTTGCGT

Downstream 100 bases:

>100_bases
GGTTGCATGTCCATCCGCACGCGTTCGCGCAAGACCCCGTCCAAGCAGCCGGAGAGCGCGCTGCGCCGCTGGCTGAAGGA
GCGCAGCATCACCGAGGTGG

Product: putative glutamine amidotransferase

Products: NA

Alternate protein names: Gamma-Glu-GABA hydrolase [H]

Number of amino acids: Translated: 251; Mature: 250

Protein sequence:

>251_residues
MAVVPLVGLPTDRILQGPHPFLAAGEKYIRAVIEAAAAMPVLLPSLQPALDAGAWLERLDGLLLTGAVSNVEPHHYSDEP
SWEGNPHDPARDATILALIPQALARGLPVLAICRGLQEVNVALGGRLHQRVHDVPGLADHREDRGTPIDMQYGPAHVVHL
HPGGWLAGMSDSAQVWVNSLHGQGIATLAEGLLIEATAPDGLIEAFRGSGPGFLLAVQWHPEWRVTQHPFYRAIFQAFGE
ACRQYAARRGK

Sequences:

>Translated_251_residues
MAVVPLVGLPTDRILQGPHPFLAAGEKYIRAVIEAAAAMPVLLPSLQPALDAGAWLERLDGLLLTGAVSNVEPHHYSDEP
SWEGNPHDPARDATILALIPQALARGLPVLAICRGLQEVNVALGGRLHQRVHDVPGLADHREDRGTPIDMQYGPAHVVHL
HPGGWLAGMSDSAQVWVNSLHGQGIATLAEGLLIEATAPDGLIEAFRGSGPGFLLAVQWHPEWRVTQHPFYRAIFQAFGE
ACRQYAARRGK
>Mature_250_residues
AVVPLVGLPTDRILQGPHPFLAAGEKYIRAVIEAAAAMPVLLPSLQPALDAGAWLERLDGLLLTGAVSNVEPHHYSDEPS
WEGNPHDPARDATILALIPQALARGLPVLAICRGLQEVNVALGGRLHQRVHDVPGLADHREDRGTPIDMQYGPAHVVHLH
PGGWLAGMSDSAQVWVNSLHGQGIATLAEGLLIEATAPDGLIEAFRGSGPGFLLAVQWHPEWRVTQHPFYRAIFQAFGEA
CRQYAARRGK

Specific function: Involved in the breakdown of putrescine via hydrolysis of the gamma-glutamyl linkage of gamma-glutamyl-gamma- aminobutyrate [H]

COG id: COG2071

COG function: function code R; Predicted glutamine amidotransferases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI87081871, Length=253, Percent_Identity=40.3162055335968, Blast_Score=179, Evalue=1e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017926
- InterPro:   IPR011697 [H]

Pfam domain/function: PF07722 Peptidase_C26 [H]

EC number: =3.5.1.94 [H]

Molecular weight: Translated: 27038; Mature: 26907

Theoretical pI: Translated: 6.44; Mature: 6.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVVPLVGLPTDRILQGPHPFLAAGEKYIRAVIEAAAAMPVLLPSLQPALDAGAWLERLD
CCEECCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHC
GLLLTGAVSNVEPHHYSDEPSWEGNPHDPARDATILALIPQALARGLPVLAICRGLQEVN
CEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHH
VALGGRLHQRVHDVPGLADHREDRGTPIDMQYGPAHVVHLHPGGWLAGMSDSAQVWVNSL
HHHHHHHHHHHHCCCCCCCCHHCCCCCCEEECCCEEEEEECCCCEECCCCCCHHHHHHHH
HGQGIATLAEGLLIEATAPDGLIEAFRGSGPGFLLAVQWHPEWRVTQHPFYRAIFQAFGE
CCCHHHHHHCCEEEEECCCHHHHHHHCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHH
ACRQYAARRGK
HHHHHHHHCCC
>Mature Secondary Structure 
AVVPLVGLPTDRILQGPHPFLAAGEKYIRAVIEAAAAMPVLLPSLQPALDAGAWLERLD
CEECCCCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHC
GLLLTGAVSNVEPHHYSDEPSWEGNPHDPARDATILALIPQALARGLPVLAICRGLQEVN
CEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHH
VALGGRLHQRVHDVPGLADHREDRGTPIDMQYGPAHVVHLHPGGWLAGMSDSAQVWVNSL
HHHHHHHHHHHHCCCCCCCCHHCCCCCCEEECCCEEEEEECCCCEECCCCCCHHHHHHHH
HGQGIATLAEGLLIEATAPDGLIEAFRGSGPGFLLAVQWHPEWRVTQHPFYRAIFQAFGE
CCCHHHHHHCCEEEEECCCHHHHHHHCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHH
ACRQYAARRGK
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]