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The map label for this gene is meth [H]

Identifier: 78047159

GI number: 78047159

Start: 1810841

End: 1811971

Strand: Reverse

Name: meth [H]

Synonym: XCV1603

Alternate gene names: 78047159

Gene position: 1811971-1810841 (Counterclockwise)

Preceding gene: 78047160

Following gene: 78047158

Centisome position: 34.99

GC content: 64.1

Gene sequence:

>1131_bases
ATGACGCCTGCAGCACTCCACTCCCCCGTCGCACATGCCCTGCCCTGGCTACGTCCCGAGCGCGCAGCCAAGCTCACCGC
CGCGCTTGCCGAGCGCATCCTCATCATCGATGGCGCGATGGGCACCATGATCCAGCGCCACGATCTGCAGGAGCCCGATT
ACCGCGGCACCCGCTTTGCCGAGGGCTACGACAGTGCGCACGTGCACGGCCCCGGTTGCGACCACGCACACGTACCGCAG
GGCCACGATCTGAAGGGCAACAACGATCTGCTGCTGTTGACCCGCCCGCAGATCATTGCCGGCATTCATCGCGCCTATCT
GGATGCAGGTGCCGATCTACTGGAAACCAATACCTTCAACGCCACGTCGGTGAGCCAGGCCGACTATCACCTGGAACATC
TGGTGTACGAGTTGAACAAGGCGGGCGCGCAAGTCGCGCGCGCCTGTTGCGACGAAGTCGAGGCGTTGACTCCGCACAAA
CCGCGCTTTGTGATCGGCGTGCTCGGCCCCACCAGCCGTACCGCCTCCATCAGCCCCGATGTCAACGATCCCGGCTACCG
CAACACCAGTTTCGATGCACTGCGCGAAACCTATCGCGAGGCCATCGAGGGCTTGATCGATGGCGGCGCGGATACGCTGA
TGGTGGAAACCATCTTCGACACGCTCAACGCCAAGGCCGCGTTGTATGCGATCGAAGAGGTCTTCGAGGCACGCGGCGGG
CGTTTGCCGGTGATGGTTTCCGGCACCATCACCGACGCATCCGGGCGCACCTTGTCCGGACAGACTGCCGAAGCGTTTTA
TGCATCGGTCGCGCACGGCAGGCCGTTGTCGATCGGCTTGAATTGTGCGCTCGGCGCCAAGGATCTGCGCCCGCACGTGG
AAACCTTGGCGCAGATCGCCGACGCGTATGTCAGCGCGCATCCGAACGCCGGCTTGCCGAATGCCTTCGGCGAATACGAC
GAAACGCCGGAAGAAATGGCGTCCACGCTACGCGAATTCGCACAGGCCGGCCTACTCAACCTGGTCGGCGGCTGTTGCGG
TACCTCGCCGGACCATATCCGCGCCATCGCCGAAGCGGTCGCCGATCTGCCACCGCGGCAACTGCCTGGCGCACAAGAGC
TGGCGGCGTGA

Upstream 100 bases:

>100_bases
ATTTCGAAGTGATCTCGTTGATTGCTGTGAAGCCGGGAATGGGGAATCGGGATTCGGGAATCGCCGACCGCAGCGCCGCT
GTCTCACGGAGCATCGCATC

Downstream 100 bases:

>100_bases
TGCGTCAGGCTCACCCTCATCCACCCTTTGCGCACCTGCTCCCGCCTGCGCGAGCAGGTGGCGCGCAGCGCCGGATGAGG
GCTGCATTGCACCGCGCCCC

Product: 5-methyltetrahydrofolate-homocysteine methyltransferase (fragment)

Products: NA

Alternate protein names: 5-methyltetrahydrofolate--homocysteine methyltransferase; Methionine synthase, vitamin-B12 dependent; MS [H]

Number of amino acids: Translated: 376; Mature: 375

Protein sequence:

>376_residues
MTPAALHSPVAHALPWLRPERAAKLTAALAERILIIDGAMGTMIQRHDLQEPDYRGTRFAEGYDSAHVHGPGCDHAHVPQ
GHDLKGNNDLLLLTRPQIIAGIHRAYLDAGADLLETNTFNATSVSQADYHLEHLVYELNKAGAQVARACCDEVEALTPHK
PRFVIGVLGPTSRTASISPDVNDPGYRNTSFDALRETYREAIEGLIDGGADTLMVETIFDTLNAKAALYAIEEVFEARGG
RLPVMVSGTITDASGRTLSGQTAEAFYASVAHGRPLSIGLNCALGAKDLRPHVETLAQIADAYVSAHPNAGLPNAFGEYD
ETPEEMASTLREFAQAGLLNLVGGCCGTSPDHIRAIAEAVADLPPRQLPGAQELAA

Sequences:

>Translated_376_residues
MTPAALHSPVAHALPWLRPERAAKLTAALAERILIIDGAMGTMIQRHDLQEPDYRGTRFAEGYDSAHVHGPGCDHAHVPQ
GHDLKGNNDLLLLTRPQIIAGIHRAYLDAGADLLETNTFNATSVSQADYHLEHLVYELNKAGAQVARACCDEVEALTPHK
PRFVIGVLGPTSRTASISPDVNDPGYRNTSFDALRETYREAIEGLIDGGADTLMVETIFDTLNAKAALYAIEEVFEARGG
RLPVMVSGTITDASGRTLSGQTAEAFYASVAHGRPLSIGLNCALGAKDLRPHVETLAQIADAYVSAHPNAGLPNAFGEYD
ETPEEMASTLREFAQAGLLNLVGGCCGTSPDHIRAIAEAVADLPPRQLPGAQELAA
>Mature_375_residues
TPAALHSPVAHALPWLRPERAAKLTAALAERILIIDGAMGTMIQRHDLQEPDYRGTRFAEGYDSAHVHGPGCDHAHVPQG
HDLKGNNDLLLLTRPQIIAGIHRAYLDAGADLLETNTFNATSVSQADYHLEHLVYELNKAGAQVARACCDEVEALTPHKP
RFVIGVLGPTSRTASISPDVNDPGYRNTSFDALRETYREAIEGLIDGGADTLMVETIFDTLNAKAALYAIEEVFEARGGR
LPVMVSGTITDASGRTLSGQTAEAFYASVAHGRPLSIGLNCALGAKDLRPHVETLAQIADAYVSAHPNAGLPNAFGEYDE
TPEEMASTLREFAQAGLLNLVGGCCGTSPDHIRAIAEAVADLPPRQLPGAQELAA

Specific function: Catalyzes the transfer of a methyl group from methyl- cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate [H]

COG id: COG0646

COG function: function code E; Methionine synthase I (cobalamin-dependent), methyltransferase domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pterin-binding domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=346, Percent_Identity=54.3352601156069, Blast_Score=383, Evalue=1e-106,
Organism=Escherichia coli, GI1790450, Length=349, Percent_Identity=58.7392550143267, Blast_Score=416, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI17535405, Length=342, Percent_Identity=54.093567251462, Blast_Score=377, Evalue=1e-105,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR011005
- InterPro:   IPR003759
- InterPro:   IPR011822
- InterPro:   IPR000489
- InterPro:   IPR003726
- InterPro:   IPR004223 [H]

Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2; PF02965 Met_synt_B12; PF00809 Pterin_bind; PF02574 S-methyl_trans [H]

EC number: =2.1.1.13 [H]

Molecular weight: Translated: 40089; Mature: 39958

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS50970 HCY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPAALHSPVAHALPWLRPERAAKLTAALAERILIIDGAMGTMIQRHDLQEPDYRGTRFA
CCCHHHCCCHHHHCCCCCHHHHHHHHHHHHHHEEEEECCHHHHHHHHCCCCCCCCCCCCC
EGYDSAHVHGPGCDHAHVPQGHDLKGNNDLLLLTRPQIIAGIHRAYLDAGADLLETNTFN
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHCCCHHHHCCCCC
ATSVSQADYHLEHLVYELNKAGAQVARACCDEVEALTPHKPRFVIGVLGPTSRTASISPD
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCC
VNDPGYRNTSFDALRETYREAIEGLIDGGADTLMVETIFDTLNAKAALYAIEEVFEARGG
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHCCC
RLPVMVSGTITDASGRTLSGQTAEAFYASVAHGRPLSIGLNCALGAKDLRPHVETLAQIA
CCEEEEEEEEECCCCCCCCCCHHHHHHHHHHCCCCEEECCEEECCCHHHHHHHHHHHHHH
DAYVSAHPNAGLPNAFGEYDETPEEMASTLREFAQAGLLNLVGGCCGTSPDHIRAIAEAV
HHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
ADLPPRQLPGAQELAA
HCCCCCCCCCHHHHCC
>Mature Secondary Structure 
TPAALHSPVAHALPWLRPERAAKLTAALAERILIIDGAMGTMIQRHDLQEPDYRGTRFA
CCHHHCCCHHHHCCCCCHHHHHHHHHHHHHHEEEEECCHHHHHHHHCCCCCCCCCCCCC
EGYDSAHVHGPGCDHAHVPQGHDLKGNNDLLLLTRPQIIAGIHRAYLDAGADLLETNTFN
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHCCCHHHHCCCCC
ATSVSQADYHLEHLVYELNKAGAQVARACCDEVEALTPHKPRFVIGVLGPTSRTASISPD
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCC
VNDPGYRNTSFDALRETYREAIEGLIDGGADTLMVETIFDTLNAKAALYAIEEVFEARGG
CCCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCHHHHHHHHHHHHHHCCC
RLPVMVSGTITDASGRTLSGQTAEAFYASVAHGRPLSIGLNCALGAKDLRPHVETLAQIA
CCEEEEEEEEECCCCCCCCCCHHHHHHHHHHCCCCEEECCEEECCCHHHHHHHHHHHHHH
DAYVSAHPNAGLPNAFGEYDETPEEMASTLREFAQAGLLNLVGGCCGTSPDHIRAIAEAV
HHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHH
ADLPPRQLPGAQELAA
HCCCCCCCCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]