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The map label for this gene is dosC [H]

Identifier: 78047033

GI number: 78047033

Start: 1659307

End: 1660830

Strand: Reverse

Name: dosC [H]

Synonym: XCV1477

Alternate gene names: 78047033

Gene position: 1660830-1659307 (Counterclockwise)

Preceding gene: 78047034

Following gene: 78047032

Centisome position: 32.07

GC content: 64.9

Gene sequence:

>1524_bases
ATGCAACCAGATCTCCAGGCCGCCCTGCACTACTGCCGTAATCTGCCCTCGCCGCCCGGCGTTGCGTTGCGCATCATCGA
ACTTGCGCAGGATCCGGACGTGGACATGACCACCACGGCCAAGGTCATCGGCATGGACATGGCATTGAGCGCACGTATGC
TGCGGGTGGCCAATTCGCCGCTCTACGCCAGTCGCCGCCGCATCGACAATCTCGGCCAGGCGCTGACCATGCTCGGCCTC
AACGCGACCTTGAGCCTGGCACTGGGCTTTTCGATGGTGCACGGCGTGCGCAGCACCTTCATCGCCCACCCCTTGCACGA
GCGCATCTGGCGCCGCGCCGGCCTATGCGCGCTGGCCAGTCGCATCCTCGGCCAGGCCTACGGCATCCGCAAACCCGAAG
AATTGATGCTGGCCGGCCTGCTGCAGGACATCGGCAAGCTGGCCCTGCTGCAGGGCGCGCCCGATCTATACGGGCCGCTG
CTGGAACAGGCCGCCGACAACACCGCGCTGCTGGTTGCCGAACGCGAACAGCTGGGCGCCACGCATGCCGAAGTCGGCGC
CTGGCTGGCGCATCAGTGGCAGCTGCCGCCGTATCTGCAGAATGCGATTGCGCAGAGCGAAGAAGAACCGGCAGGCCTGG
AACCGTTCATGGCCTGCGTGGCGCTATCCGGGCGCCTGGCCGATATCTGGCTCTCGGCCAGTGCGGTCACTGCAACCGAA
CATGCGCAGCAGCAGGCGCAGGACGTGCTGGAGCTCGATGCCGAACGCTTCGAAAAAGTGATCGAACGCATCGCCGAATC
GCTGCCGGAACTGGAGGCGCTGTTCGACATCCGCGTGCCGCAGCCCGAGCACATCCAGATGATCTTCGAGCAGGCGCGTG
AACTGGCGATGCTGCGCAGCCTGCGCGAATTGCAGGACGTGGCCGAAGCGCGCCGGCATGCCGACGAATCGGAAAACCGC
ATGCGTCACCTGGCCGAACAGGCCAGCCGCGATGCGTTGACCGGGGTATTCAATCGGCATCAGCTCGGTACCGCGCTGAC
GCATGAGTTCGAACTGGCCTCGCGCCAGGGCTGGCCGCTGTCGATCGCCTTCATCGACCTGGACGAGTTCAAGCGCGTCA
ACGATGCGCACGGACATCTGGTCGGCGATGAAGTGTTGCGCAATTTCGCGCAAACGCTGCAACGCATGGTGCGCACCAGC
GACCTGGTGGCGCGCTACGGCGGCGAAGAATTCCTGGTGATCCTGCCCAATACACCGGCCGATGCGGCGCTGATGGTGAT
CGAACGCATCCTGGCCGAAACCGCGCGTACGCCGATGGCGCAACTGAAGGGCGGCCCATTGCATATCACCTTCTCTGCGG
GTCTTGCCACGCACGGCGGTGCCGAGCGCCAGTTCGACAGCATCGACCATTTGTTGCAGGCCGCCGACAGCACACTGTAT
CGCTCCAAGCATCGCGGCCGCAACCGCGTCACTGCGTACGATGCGACCGATGTACCAACGCCGCCGAACCCGCGCGCGCA
TTGA

Upstream 100 bases:

>100_bases
GTTCCATCACGCATGTTTTGTGGCGCGGCGATGGCGGAGCATGGCGCGCCCTACTCAACGACCGCAGCTGTATGCTTGTG
GCCACTGTTGGCGATGACTC

Downstream 100 bases:

>100_bases
GCAACCGGTCACGCCACTGCCAACAAAGGAATTTTTACCACGCCAGGTGACAGGCTTCCGCTAGAATTGCCGGCGTTTGC
CGATCCCCCACCCCCGAGGT

Product: putative signal transduction protein

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 507; Mature: 507

Protein sequence:

>507_residues
MQPDLQAALHYCRNLPSPPGVALRIIELAQDPDVDMTTTAKVIGMDMALSARMLRVANSPLYASRRRIDNLGQALTMLGL
NATLSLALGFSMVHGVRSTFIAHPLHERIWRRAGLCALASRILGQAYGIRKPEELMLAGLLQDIGKLALLQGAPDLYGPL
LEQAADNTALLVAEREQLGATHAEVGAWLAHQWQLPPYLQNAIAQSEEEPAGLEPFMACVALSGRLADIWLSASAVTATE
HAQQQAQDVLELDAERFEKVIERIAESLPELEALFDIRVPQPEHIQMIFEQARELAMLRSLRELQDVAEARRHADESENR
MRHLAEQASRDALTGVFNRHQLGTALTHEFELASRQGWPLSIAFIDLDEFKRVNDAHGHLVGDEVLRNFAQTLQRMVRTS
DLVARYGGEEFLVILPNTPADAALMVIERILAETARTPMAQLKGGPLHITFSAGLATHGGAERQFDSIDHLLQAADSTLY
RSKHRGRNRVTAYDATDVPTPPNPRAH

Sequences:

>Translated_507_residues
MQPDLQAALHYCRNLPSPPGVALRIIELAQDPDVDMTTTAKVIGMDMALSARMLRVANSPLYASRRRIDNLGQALTMLGL
NATLSLALGFSMVHGVRSTFIAHPLHERIWRRAGLCALASRILGQAYGIRKPEELMLAGLLQDIGKLALLQGAPDLYGPL
LEQAADNTALLVAEREQLGATHAEVGAWLAHQWQLPPYLQNAIAQSEEEPAGLEPFMACVALSGRLADIWLSASAVTATE
HAQQQAQDVLELDAERFEKVIERIAESLPELEALFDIRVPQPEHIQMIFEQARELAMLRSLRELQDVAEARRHADESENR
MRHLAEQASRDALTGVFNRHQLGTALTHEFELASRQGWPLSIAFIDLDEFKRVNDAHGHLVGDEVLRNFAQTLQRMVRTS
DLVARYGGEEFLVILPNTPADAALMVIERILAETARTPMAQLKGGPLHITFSAGLATHGGAERQFDSIDHLLQAADSTLY
RSKHRGRNRVTAYDATDVPTPPNPRAH
>Mature_507_residues
MQPDLQAALHYCRNLPSPPGVALRIIELAQDPDVDMTTTAKVIGMDMALSARMLRVANSPLYASRRRIDNLGQALTMLGL
NATLSLALGFSMVHGVRSTFIAHPLHERIWRRAGLCALASRILGQAYGIRKPEELMLAGLLQDIGKLALLQGAPDLYGPL
LEQAADNTALLVAEREQLGATHAEVGAWLAHQWQLPPYLQNAIAQSEEEPAGLEPFMACVALSGRLADIWLSASAVTATE
HAQQQAQDVLELDAERFEKVIERIAESLPELEALFDIRVPQPEHIQMIFEQARELAMLRSLRELQDVAEARRHADESENR
MRHLAEQASRDALTGVFNRHQLGTALTHEFELASRQGWPLSIAFIDLDEFKRVNDAHGHLVGDEVLRNFAQTLQRMVRTS
DLVARYGGEEFLVILPNTPADAALMVIERILAETARTPMAQLKGGPLHITFSAGLATHGGAERQFDSIDHLLQAADSTLY
RSKHRGRNRVTAYDATDVPTPPNPRAH

Specific function: Overexpression leads to an increased level of c-di-GMP, which leads to changes in the cell surface, to abnormal cell division, increased biofilm formation and decreased swimming (the latter 2 in strain W3110). In a strain able to produce cellulose (strain

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=181, Percent_Identity=38.6740331491713, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI87081881, Length=192, Percent_Identity=41.6666666666667, Blast_Score=120, Evalue=3e-28,
Organism=Escherichia coli, GI1786584, Length=173, Percent_Identity=35.2601156069364, Blast_Score=105, Evalue=7e-24,
Organism=Escherichia coli, GI1787802, Length=222, Percent_Identity=32.4324324324324, Blast_Score=102, Evalue=8e-23,
Organism=Escherichia coli, GI1787262, Length=189, Percent_Identity=33.8624338624339, Blast_Score=99, Evalue=5e-22,
Organism=Escherichia coli, GI1788381, Length=201, Percent_Identity=34.3283582089552, Blast_Score=99, Evalue=7e-22,
Organism=Escherichia coli, GI87082007, Length=172, Percent_Identity=36.6279069767442, Blast_Score=89, Evalue=7e-19,
Organism=Escherichia coli, GI1787816, Length=186, Percent_Identity=31.7204301075269, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1787541, Length=188, Percent_Identity=30.8510638297872, Blast_Score=86, Evalue=7e-18,
Organism=Escherichia coli, GI87081974, Length=168, Percent_Identity=29.7619047619048, Blast_Score=73, Evalue=4e-14,
Organism=Escherichia coli, GI87081977, Length=178, Percent_Identity=32.0224719101124, Blast_Score=72, Evalue=9e-14,
Organism=Escherichia coli, GI1788085, Length=173, Percent_Identity=28.9017341040462, Blast_Score=66, Evalue=6e-12,
Organism=Escherichia coli, GI1787056, Length=173, Percent_Identity=26.5895953757225, Blast_Score=65, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160
- InterPro:   IPR012292
- InterPro:   IPR009050 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 56015; Mature: 56015

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQPDLQAALHYCRNLPSPPGVALRIIELAQDPDVDMTTTAKVIGMDMALSARMLRVANSP
CCCHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
LYASRRRIDNLGQALTMLGLNATLSLALGFSMVHGVRSTFIAHPLHERIWRRAGLCALAS
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RILGQAYGIRKPEELMLAGLLQDIGKLALLQGAPDLYGPLLEQAADNTALLVAEREQLGA
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEEHHHHCC
THAEVGAWLAHQWQLPPYLQNAIAQSEEEPAGLEPFMACVALSGRLADIWLSASAVTATE
HHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHCCCHHHHHCCCHHHHHHH
HAQQQAQDVLELDAERFEKVIERIAESLPELEALFDIRVPQPEHIQMIFEQARELAMLRS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
LRELQDVAEARRHADESENRMRHLAEQASRDALTGVFNRHQLGTALTHEFELASRQGWPL
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
SIAFIDLDEFKRVNDAHGHLVGDEVLRNFAQTLQRMVRTSDLVARYGGEEFLVILPNTPA
EEEEEEHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCC
DAALMVIERILAETARTPMAQLKGGPLHITFSAGLATHGGAERQFDSIDHLLQAADSTLY
HHHHHHHHHHHHHHHCCCHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHH
RSKHRGRNRVTAYDATDVPTPPNPRAH
HHHHCCCCCEEEECCCCCCCCCCCCCC
>Mature Secondary Structure
MQPDLQAALHYCRNLPSPPGVALRIIELAQDPDVDMTTTAKVIGMDMALSARMLRVANSP
CCCHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
LYASRRRIDNLGQALTMLGLNATLSLALGFSMVHGVRSTFIAHPLHERIWRRAGLCALAS
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RILGQAYGIRKPEELMLAGLLQDIGKLALLQGAPDLYGPLLEQAADNTALLVAEREQLGA
HHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCEEEEEEHHHHCC
THAEVGAWLAHQWQLPPYLQNAIAQSEEEPAGLEPFMACVALSGRLADIWLSASAVTATE
HHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCHHHHHHHHHHCCCHHHHHCCCHHHHHHH
HAQQQAQDVLELDAERFEKVIERIAESLPELEALFDIRVPQPEHIQMIFEQARELAMLRS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
LRELQDVAEARRHADESENRMRHLAEQASRDALTGVFNRHQLGTALTHEFELASRQGWPL
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
SIAFIDLDEFKRVNDAHGHLVGDEVLRNFAQTLQRMVRTSDLVARYGGEEFLVILPNTPA
EEEEEEHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCC
DAALMVIERILAETARTPMAQLKGGPLHITFSAGLATHGGAERQFDSIDHLLQAADSTLY
HHHHHHHHHHHHHHHCCCHHHHCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHH
RSKHRGRNRVTAYDATDVPTPPNPRAH
HHHHCCCCCEEEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]