Click here to switch to the map view.

The map label for this gene is yitJ [H]

Identifier: 78044792

GI number: 78044792

Start: 1099808

End: 1100677

Strand: Direct

Name: yitJ [H]

Synonym: CHY_1233

Alternate gene names: 78044792

Gene position: 1099808-1100677 (Clockwise)

Preceding gene: 78044586

Following gene: 78043027

Centisome position: 45.8

GC content: 41.15

Gene sequence:

>870_bases
GTGGTTACGGCAGAAATTGGACCTCCCAAAAACAGTAACGGAGAAGTAGTACGCAAACATGCTCGTAACTTAAAGAATTA
CATTGATGGAGCAAACTTAACCGATAACCAAACTGCGGTGGTTAGATTATCCAGTATTGCCGCTGGTTTTCATGTATTAT
CCGAGGGAGTTGAACCAATCATTCAAATGACCTGCCGGGATAGAAATCGCATTGGGCTTCAATCGGACTTATTAGGAGCG
TATAGTCTTGGAATACGCAATGTTTTGGCCCTGACCGGGGACCACCAATCGTTCGGTAACCATCCTACTGCTAAAAACGT
CTACGATTTGGATTCGATCCAGTTAATTCAAACCATCAAAAAATTAAGAGATGAAAAACAATTTTTATCCGGTGAACCTA
TTAAAGAACATGAACCGCGCTTTTTCATTGGCGCCGTAGAAAATCCTTTTGGTGATCCCTTTGAGTTTCGAGTTTTACGC
CTTGAGAAAAAAATAGAAGCGGGGGCACAGTTTATTCAAACCCAGTGTATCTTTGATATGGAAAGATTTGAAAGATTTAT
GGAAATGGTTAGAGAAAGAGGGCTCCACGAAAGGGCGTATATTTTAGCAGGTATTACGCCATTAAAATCAGCGAAAGCTG
CTAAGTATATGAAAAACGTTGCCGGAATGATAATGCCTGACCATATAATAGAACGGATGGAGAAAGCCGAAGACCAAAAA
GAAGAAGGGGTAAAAATTGCCGTTGAAATGATTGAACACTTAAAGAATGTTAAAGGCGTTGCTGGGGTTCATATCATGGC
AATTGCCTGGGAAGATATTGTTCCGGTTATTGTGGAAAGAGCTGGACTGCTTCCACGGCCTGAAGTTTAA

Upstream 100 bases:

>100_bases
CTAAAGCCCGGGATGGTGGTCCCAGGAAAGTAATTAAGGAGGAGGTCCGGTTATGATAACGGAAAGCCGCCTGGAAAAAT
TATTTGCCGAAGGTCATTTT

Downstream 100 bases:

>100_bases
ACTTCAGGCTTTTTTCTTTTAAACTTCCTGATTTTCTGATATATTATTATTGCGAGATTGGAGGGGTAGGATTGCAGGAT
TACCATATACACCCCGGTTA

Product: methylenetetrahydrofolate reductase family protein

Products: NA

Alternate protein names: Homocysteine S-methyltransferase; S-methylmethionine:homocysteine methyltransferase; 5,10-methylenetetrahydrofolate reductase [H]

Number of amino acids: Translated: 289; Mature: 289

Protein sequence:

>289_residues
MVTAEIGPPKNSNGEVVRKHARNLKNYIDGANLTDNQTAVVRLSSIAAGFHVLSEGVEPIIQMTCRDRNRIGLQSDLLGA
YSLGIRNVLALTGDHQSFGNHPTAKNVYDLDSIQLIQTIKKLRDEKQFLSGEPIKEHEPRFFIGAVENPFGDPFEFRVLR
LEKKIEAGAQFIQTQCIFDMERFERFMEMVRERGLHERAYILAGITPLKSAKAAKYMKNVAGMIMPDHIIERMEKAEDQK
EEGVKIAVEMIEHLKNVKGVAGVHIMAIAWEDIVPVIVERAGLLPRPEV

Sequences:

>Translated_289_residues
MVTAEIGPPKNSNGEVVRKHARNLKNYIDGANLTDNQTAVVRLSSIAAGFHVLSEGVEPIIQMTCRDRNRIGLQSDLLGA
YSLGIRNVLALTGDHQSFGNHPTAKNVYDLDSIQLIQTIKKLRDEKQFLSGEPIKEHEPRFFIGAVENPFGDPFEFRVLR
LEKKIEAGAQFIQTQCIFDMERFERFMEMVRERGLHERAYILAGITPLKSAKAAKYMKNVAGMIMPDHIIERMEKAEDQK
EEGVKIAVEMIEHLKNVKGVAGVHIMAIAWEDIVPVIVERAGLLPRPEV
>Mature_289_residues
MVTAEIGPPKNSNGEVVRKHARNLKNYIDGANLTDNQTAVVRLSSIAAGFHVLSEGVEPIIQMTCRDRNRIGLQSDLLGA
YSLGIRNVLALTGDHQSFGNHPTAKNVYDLDSIQLIQTIKKLRDEKQFLSGEPIKEHEPRFFIGAVENPFGDPFEFRVLR
LEKKIEAGAQFIQTQCIFDMERFERFMEMVRERGLHERAYILAGITPLKSAKAAKYMKNVAGMIMPDHIIERMEKAEDQK
EEGVKIAVEMIEHLKNVKGVAGVHIMAIAWEDIVPVIVERAGLLPRPEV

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Hcy-binding domain [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=303, Percent_Identity=23.7623762376238, Blast_Score=73, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI25147436, Length=257, Percent_Identity=26.8482490272374, Blast_Score=88, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI25147441, Length=257, Percent_Identity=26.8482490272374, Blast_Score=88, Evalue=6e-18,
Organism=Saccharomyces cerevisiae, GI6325234, Length=239, Percent_Identity=25.5230125523013, Blast_Score=66, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR003726 [H]

Pfam domain/function: PF02219 MTHFR; PF02574 S-methyl_trans [H]

EC number: =2.1.1.10; =1.5.1.20 [H]

Molecular weight: Translated: 32508; Mature: 32508

Theoretical pI: Translated: 7.03; Mature: 7.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HCCHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure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HCCHHHHHHHHHHHHHCCCCCCCEEEEHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9353932; 9384377 [H]