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The map label for this gene is mhpE

Identifier: 78044450

GI number: 78044450

Start: 1140042

End: 1141052

Strand: Direct

Name: mhpE

Synonym: CHY_1280

Alternate gene names: 78044450

Gene position: 1140042-1141052 (Clockwise)

Preceding gene: 78042871

Following gene: 78042656

Centisome position: 47.47

GC content: 41.84

Gene sequence:

>1011_bases
ATGGCTAAAAAGATTAGGATAATTGACAGTACCTTAAGAGATGGAATGCATGCGGTAAGTCACCAGTTCACACCCGAGGA
AATGGCAGAAATTGCAGCAGGACTGGATGCGGCAGGGATTGACACCATTGAAGTGAGCCATGGTGATGGATTAGGTGGTT
CGAGCTACAATTATGGATTTGCGGCTGCAAAAGATGAAGATTATTTAAAAGCGGTATCTCAAGTTTTAAAAAATTCAAAG
TTAGGTGTCCTTTTGCTGCCAGGAATTGGTACTGCTCATGATTTAGAGATGGCTGCAAAATTTGGTGCAAAAGTTGTGCG
AGTTGCTACCCACTGTACGGAAGCCGATATTGGCGAACAGCATATCAAGATTGCCAAAGAATTAGGAATGGAAGCCATTG
GATTTCTAATGATGAGCCATATGGTTCCTCCAGAAAAACTTGTGGAGCAAGCGAAATTGTTTGAAAGTTATGGAGCAGAT
GCGGTTTATATTACAGATTCTGCTGGAGCAATGACTCCGTATGATGTTAAGGTGCGCATTGAAGCGGTTAAAGCTGCTGT
TAGCGTGCCTGTTGGGTTTCATGCTCACAATAATTTAGGGTTAGCGATTGGTAATACTCTGGCAGCTATCGAAGCTGGAG
CAACTTATGTTGACGGCACCGCGCGAGGACTTGGAGCAGGGGCCGGAAATTCACAAACGGAAATTTTGGTTGCTGTTCTG
GCGAAGTTAGGTTATGAAACTGGTGTTGATTTGTATAAAATTATGGATGTAGCAGAAGAAGTTGTTGCTCCTAAAATGAG
AAGACCGCAAATTGTTGATAAAGCGGCATTAAGTTTAGGTTATGCGGGTGTTTACGGAAGCTTTCTCTTACATGCTATGC
GGGCCGCAGAAAAGTTTAAAGTTGATGTTCGTGATATTTTAATCGAGTTAGGAAGATTAAAAACTGTTGGCGGGCAAGAA
GATATGATAGTTGATGTTGCCTATGAATTAAGTAAAGCAAATAAGCAATAA

Upstream 100 bases:

>100_bases
GCCGGTAATCTTGATATAATCACCGCAGCAGCAGTAGCTTTTGCTGATGAAGTAGCTAAAAAGTTGCTGGCTGAGGAGCA
AGCTAAGGAGGTTAGAGCAC

Downstream 100 bases:

>100_bases
ATGTTTTTTTATTTCAATTAATAAAAACAGAATACGACAATGATGAATAGCTTGAACAATTATTGGAAAGCAGGCCAGAA
GAACTCTGGCCTGCTTTAGC

Product: 4-hydroxy-2-ketovalerate aldolase

Products: NA

Alternate protein names: HOA; 4-hydroxy-2-keto-pentanoic acid aldolase; 4-hydroxy-2-oxopentanoate aldolase

Number of amino acids: Translated: 336; Mature: 335

Protein sequence:

>336_residues
MAKKIRIIDSTLRDGMHAVSHQFTPEEMAEIAAGLDAAGIDTIEVSHGDGLGGSSYNYGFAAAKDEDYLKAVSQVLKNSK
LGVLLLPGIGTAHDLEMAAKFGAKVVRVATHCTEADIGEQHIKIAKELGMEAIGFLMMSHMVPPEKLVEQAKLFESYGAD
AVYITDSAGAMTPYDVKVRIEAVKAAVSVPVGFHAHNNLGLAIGNTLAAIEAGATYVDGTARGLGAGAGNSQTEILVAVL
AKLGYETGVDLYKIMDVAEEVVAPKMRRPQIVDKAALSLGYAGVYGSFLLHAMRAAEKFKVDVRDILIELGRLKTVGGQE
DMIVDVAYELSKANKQ

Sequences:

>Translated_336_residues
MAKKIRIIDSTLRDGMHAVSHQFTPEEMAEIAAGLDAAGIDTIEVSHGDGLGGSSYNYGFAAAKDEDYLKAVSQVLKNSK
LGVLLLPGIGTAHDLEMAAKFGAKVVRVATHCTEADIGEQHIKIAKELGMEAIGFLMMSHMVPPEKLVEQAKLFESYGAD
AVYITDSAGAMTPYDVKVRIEAVKAAVSVPVGFHAHNNLGLAIGNTLAAIEAGATYVDGTARGLGAGAGNSQTEILVAVL
AKLGYETGVDLYKIMDVAEEVVAPKMRRPQIVDKAALSLGYAGVYGSFLLHAMRAAEKFKVDVRDILIELGRLKTVGGQE
DMIVDVAYELSKANKQ
>Mature_335_residues
AKKIRIIDSTLRDGMHAVSHQFTPEEMAEIAAGLDAAGIDTIEVSHGDGLGGSSYNYGFAAAKDEDYLKAVSQVLKNSKL
GVLLLPGIGTAHDLEMAAKFGAKVVRVATHCTEADIGEQHIKIAKELGMEAIGFLMMSHMVPPEKLVEQAKLFESYGADA
VYITDSAGAMTPYDVKVRIEAVKAAVSVPVGFHAHNNLGLAIGNTLAAIEAGATYVDGTARGLGAGAGNSQTEILVAVLA
KLGYETGVDLYKIMDVAEEVVAPKMRRPQIVDKAALSLGYAGVYGSFLLHAMRAAEKFKVDVRDILIELGRLKTVGGQED
MIVDVAYELSKANKQ

Specific function: Catalyzes the retro-aldol cleavage of 4-hydroxy-2- oxopentanoate to pyruvate and acetaldehyde. Is involved in the meta-cleavage pathway for the degradation of aromatic compounds

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 4-hydroxy-2-oxovalerate aldolase family

Homologues:

Organism=Homo sapiens, GI109150427, Length=159, Percent_Identity=30.188679245283, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI109150422, Length=159, Percent_Identity=30.188679245283, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI62198232, Length=289, Percent_Identity=25.9515570934256, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1786548, Length=335, Percent_Identity=53.4328358208955, Blast_Score=354, Evalue=6e-99,
Organism=Escherichia coli, GI1786261, Length=317, Percent_Identity=25.2365930599369, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HOA_CARHZ (Q3ACM0)

Other databases:

- EMBL:   CP000141
- RefSeq:   YP_360114.1
- HSSP:   P35914
- ProteinModelPortal:   Q3ACM0
- SMR:   Q3ACM0
- STRING:   Q3ACM0
- GeneID:   3727568
- GenomeReviews:   CP000141_GR
- KEGG:   chy:CHY_1280
- NMPDR:   fig|246194.3.peg.1319
- TIGR:   CHY_1280
- eggNOG:   COG0119
- HOGENOM:   HBG300122
- OMA:   GMALANS
- ProtClustDB:   PRK08195
- BioCyc:   CHYD246194:CHY_1280-MONOMER
- HAMAP:   MF_01656
- InterPro:   IPR017629
- InterPro:   IPR013785
- InterPro:   IPR012425
- InterPro:   IPR000891
- Gene3D:   G3DSA:3.20.20.70
- ProDom:   PD005364
- TIGRFAMs:   TIGR03217

Pfam domain/function: PF07836 DmpG_comm; PF00682 HMGL-like

EC number: =4.1.3.39

Molecular weight: Translated: 35633; Mature: 35502

Theoretical pI: Translated: 5.60; Mature: 5.60

Prosite motif: PS50991 PYR_CT

Important sites: BINDING 167-167 BINDING 194-194 BINDING 285-285

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKIRIIDSTLRDGMHAVSHQFTPEEMAEIAAGLDAAGIDTIEVSHGDGLGGSSYNYGF
CCCEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCE
AAAKDEDYLKAVSQVLKNSKLGVLLLPGIGTAHDLEMAAKFGAKVVRVATHCTEADIGEQ
ECCCCHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
HIKIAKELGMEAIGFLMMSHMVPPEKLVEQAKLFESYGADAVYITDSAGAMTPYDVKVRI
HHHHHHHHCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCEEEEEH
EAVKAAVSVPVGFHAHNNLGLAIGNTLAAIEAGATYVDGTARGLGAGAGNSQTEILVAVL
HHHHHHHHCCCEEECCCCCCEECCCHHHHHHCCCEEECCCCCCCCCCCCCCHHHHHHHHH
AKLGYETGVDLYKIMDVAEEVVAPKMRRPQIVDKAALSLGYAGVYGSFLLHAMRAAEKFK
HHHCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VDVRDILIELGRLKTVGGQEDMIVDVAYELSKANKQ
HHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHCCCC
>Mature Secondary Structure 
AKKIRIIDSTLRDGMHAVSHQFTPEEMAEIAAGLDAAGIDTIEVSHGDGLGGSSYNYGF
CCEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCE
AAAKDEDYLKAVSQVLKNSKLGVLLLPGIGTAHDLEMAAKFGAKVVRVATHCTEADIGEQ
ECCCCHHHHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
HIKIAKELGMEAIGFLMMSHMVPPEKLVEQAKLFESYGADAVYITDSAGAMTPYDVKVRI
HHHHHHHHCHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCEEEEEH
EAVKAAVSVPVGFHAHNNLGLAIGNTLAAIEAGATYVDGTARGLGAGAGNSQTEILVAVL
HHHHHHHHCCCEEECCCCCCEECCCHHHHHHCCCEEECCCCCCCCCCCCCCHHHHHHHHH
AKLGYETGVDLYKIMDVAEEVVAPKMRRPQIVDKAALSLGYAGVYGSFLLHAMRAAEKFK
HHHCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VDVRDILIELGRLKTVGGQEDMIVDVAYELSKANKQ
HHHHHHHHHHHHHCCCCCCCCEEEHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA