Click here to switch to the map view.

The map label for this gene is hisH

Identifier: 78043511

GI number: 78043511

Start: 964396

End: 964995

Strand: Direct

Name: hisH

Synonym: CHY_1088

Alternate gene names: 78043511

Gene position: 964396-964995 (Clockwise)

Preceding gene: 78042790

Following gene: 78043937

Centisome position: 40.16

GC content: 45.5

Gene sequence:

>600_bases
GTGACGGTTGTGGTAGATTACGAAATGGGTAATCTTTTAAGTGTGACCAAGGCTTTAGAAGAACTGGGATATAAACCTTC
CGTTACTTCGGATCCCAGGAAAATTTTGGAGGAGGATTTGGTGGTTTTACCCGGGGTTGGGGCGTTCAGGGATGCGGTGA
GGAATTTAAAGGAAAAGGGGCTTTTTTTGGCTTTAAAAGAACGGGCTTCCTTAAACCGGCCAATCCTGGGGATTTGTCTG
GGGATGCAGCTTTTTTTTACGAAAAGCTATGAAGATGGCGAGTATGAGGGTTTGGACCTTATTCCCGGGGAAGTGGTTCG
TTTTCAAAAAGCTCCCAAAATTCCCCACATGGGGTGGAATAACTTGGTACCGGTAGACACGACCCATGAACTTTTTAAGA
ATTTACCGGATTATTATGTTTATTTTGTCCACTCCTACTATGCCCAGACCGACTCCAGGTATGTTTTAGCTTATACCGAA
TACGGGGAAAAGTTTCCGGCGGCGGTAAGGCGGGGCAGTATTATTGGCTTTCAGTTTCATCCGGAAAAAAGCGGACCGGT
GGGAAGGCAGATACTTAAGAATTTGAGGGAGATGCTATGA

Upstream 100 bases:

>100_bases
TAAAGCCGAGGCAATTTTTAAAGGCTTGGGACGTACCTTAAAAGTTGCGGTTTCCGTCCAGGGGGATGAATTACCCTCAA
CCAAAGGAGTGATTTAGAAA

Downstream 100 bases:

>100_bases
TTTTAATACCAGCAATAGATTTAATGGGGAAAAAAGTGGTGCGGCTTACGCAGGGGGAAAAAGAGCGGAAAACCGAATAC
CCGATTTCGCCCGTGGAGCT

Product: imidazole glycerol phosphate synthase, glutamine amidotransferase subunit

Products: NA

Alternate protein names: IGP synthase glutamine amidotransferase subunit; IGP synthase subunit hisH; ImGP synthase subunit hisH; IGPS subunit hisH

Number of amino acids: Translated: 199; Mature: 198

Protein sequence:

>199_residues
MTVVVDYEMGNLLSVTKALEELGYKPSVTSDPRKILEEDLVVLPGVGAFRDAVRNLKEKGLFLALKERASLNRPILGICL
GMQLFFTKSYEDGEYEGLDLIPGEVVRFQKAPKIPHMGWNNLVPVDTTHELFKNLPDYYVYFVHSYYAQTDSRYVLAYTE
YGEKFPAAVRRGSIIGFQFHPEKSGPVGRQILKNLREML

Sequences:

>Translated_199_residues
MTVVVDYEMGNLLSVTKALEELGYKPSVTSDPRKILEEDLVVLPGVGAFRDAVRNLKEKGLFLALKERASLNRPILGICL
GMQLFFTKSYEDGEYEGLDLIPGEVVRFQKAPKIPHMGWNNLVPVDTTHELFKNLPDYYVYFVHSYYAQTDSRYVLAYTE
YGEKFPAAVRRGSIIGFQFHPEKSGPVGRQILKNLREML
>Mature_198_residues
TVVVDYEMGNLLSVTKALEELGYKPSVTSDPRKILEEDLVVLPGVGAFRDAVRNLKEKGLFLALKERASLNRPILGICLG
MQLFFTKSYEDGEYEGLDLIPGEVVRFQKAPKIPHMGWNNLVPVDTTHELFKNLPDYYVYFVHSYYAQTDSRYVLAYTEY
GEKFPAAVRRGSIIGFQFHPEKSGPVGRQILKNLREML

Specific function: IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The hisH subunit provides the glutamine amidotransferase activity that produces the ammonia necessary to hisF for the synthesis of IGP and AICAR

COG id: COG0118

COG function: function code E; Glutamine amidotransferase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain

Homologues:

Organism=Escherichia coli, GI1788334, Length=199, Percent_Identity=40.2010050251256, Blast_Score=139, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6319725, Length=206, Percent_Identity=36.8932038834951, Blast_Score=124, Evalue=9e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HIS5_CARHZ (Q3AD50)

Other databases:

- EMBL:   CP000141
- RefSeq:   YP_359934.1
- ProteinModelPortal:   Q3AD50
- SMR:   Q3AD50
- STRING:   Q3AD50
- GeneID:   3726560
- GenomeReviews:   CP000141_GR
- KEGG:   chy:CHY_1088
- NMPDR:   fig|246194.3.peg.1135
- TIGR:   CHY_1088
- eggNOG:   COG0118
- HOGENOM:   HBG292341
- OMA:   RPFFGIC
- BioCyc:   CHYD246194:CHY_1088-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00278
- InterPro:   IPR017926
- InterPro:   IPR000991
- InterPro:   IPR010139
- InterPro:   IPR016226
- PIRSF:   PIRSF000495
- TIGRFAMs:   TIGR01855

Pfam domain/function: PF00117 GATase

EC number: 2.4.2.-

Molecular weight: Translated: 22654; Mature: 22523

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: PS51273 GATASE_TYPE_1; PS00442 GATASE_TYPE_I

Important sites: ACT_SITE 79-79 ACT_SITE 180-180 ACT_SITE 182-182

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVVVDYEMGNLLSVTKALEELGYKPSVTSDPRKILEEDLVVLPGVGAFRDAVRNLKEKG
CEEEEEECCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHCCEEECCCHHHHHHHHHHHHCC
LFLALKERASLNRPILGICLGMQLFFTKSYEDGEYEGLDLIPGEVVRFQKAPKIPHMGWN
EEEEEHHHHCCCCCHHHHHHHHHHEEECCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCC
NLVPVDTTHELFKNLPDYYVYFVHSYYAQTDSRYVLAYTEYGEKFPAAVRRGSIIGFQFH
CCCCCCCHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHCCCEEEEEEC
PEKSGPVGRQILKNLREML
CCCCCCHHHHHHHHHHHHC
>Mature Secondary Structure 
TVVVDYEMGNLLSVTKALEELGYKPSVTSDPRKILEEDLVVLPGVGAFRDAVRNLKEKG
EEEEEECCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHCCEEECCCHHHHHHHHHHHHCC
LFLALKERASLNRPILGICLGMQLFFTKSYEDGEYEGLDLIPGEVVRFQKAPKIPHMGWN
EEEEEHHHHCCCCCHHHHHHHHHHEEECCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCC
NLVPVDTTHELFKNLPDYYVYFVHSYYAQTDSRYVLAYTEYGEKFPAAVRRGSIIGFQFH
CCCCCCCHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHCCCEEEEEEC
PEKSGPVGRQILKNLREML
CCCCCCHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Glycosyltransferases; Pentosyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA