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The map label for this gene is purF [H]

Identifier: 78043394

GI number: 78043394

Start: 951325

End: 952683

Strand: Direct

Name: purF [H]

Synonym: CHY_1075

Alternate gene names: 78043394

Gene position: 951325-952683 (Clockwise)

Preceding gene: 78044209

Following gene: 78044305

Centisome position: 39.61

GC content: 47.83

Gene sequence:

>1359_bases
TTGGGCTTTGAAATAAGAGAAGAGTGCGGGGTGTTTGGAATTTATGCCCCGGGGCATGATGTGGCCCGGCTTACCTACTG
GGGACTTTTTGCCCTGCAGCACCGGGGGCAGGAAAGCGCGGGAATTGCGGTAGGCGATGGCAGGGATATAAACCTTCACA
AGGGCATGGGGCTGGTGGCGGAAGTTTTTAGCGAGGAGAAATTAGACGGGTTAAAAGGATTTGTGGCCGCCGGACATGTA
CGCTATTCGACCACCGGGGCCAGCGTGCCCATAAATGCCCAGCCGTTGGTATTTCATTATAAGTTGGGAAAAATTGCTTT
AGGACATAACGGCAATCTCGCCAACGTTTTGGAGTTAAAGAAAAATCTTCTGTCCCGGGGGGCAATTTTTCAGACCACTA
CCGACAGCGAAGTGATTTTAAATGTTATTGCCCAGTACGCCCAGACGGATTTGGTGGAAGGTATTTTAAAGGCGATGGTG
GATTTAAAGGGAGCGTATTCTTTGGTGATTATGACCGAGAACCAATTAATAGGGGTGCGCGATCCCTGGGGTTTTAGGCC
TTTGGTTTTGGGGAGATTTTACAACGGTTATGTTTTAGCTTCCGAGTCGGCAGCCCTTGATACCATTGGCGCTGAGCTTA
TTCGGGATGTGGAGCCGGGGGAAATTATCGTAATTGACGAAAACGGCCTTAAAAGTTATAAATTCCTGCAGGGCATGAAG
CATTCCTTTTGCATTTTTGAATTCATCTATTTTGCCCGACCGGACAGCATTATTAATGGCTATCATGTAAACACCGTTCG
CCGGAAAATGGGACAGGTTTTGGCCAAGGAATACCCGGTAAAGGCGGATTTGGTGGTGCCGGTGCCGGATTCGGGTACCG
CCGCAGCCCGGGGGTATGCGGAGGCATCGGGGATACCTCTGGAAGAGGGGTTAATGAAAAATCGTTATGCCGGCCGCACT
TTTATCCGGCCGAGCCAAAGAGAACGGGAAATTGGCGTGAAGTTAAAGCTAAACCCTATCCGGGAGGTTTTAAAGGACAA
AAAGATTGTGGTTATTGATGATTCGATTGTCCGGGGGACGACCAGCAAGCAGATTATCGAGCTTTTAAGAGATGCCGGGG
CCAGGGAAATTCATTTATTGTTAAGTTCGCCGCCGTTTATACGCTCCTGTTACTACGGTATTGATATTTCCCGTGACGAT
GAATTAATTGCGGCAAAATATTCGGTTGAGGAAATAAAAAACTATCTTGGGGCCGACGGACTTTATTATTTGTCTTTAGA
AGGCTTACTTGGGATTTTTAAGGAACGGGATTTTTGTCTTGCTTGCTTTACCGGGGATTACCCGGTACCGATAAATTAA

Upstream 100 bases:

>100_bases
GGGTGAACGGGAAGGATGAGATAGAAATTTGCCTAAACGGTCAGAGGGTCATCCAAGAAAAGCTATCGGAGTTAAAAGCC
TTGTATGAGGGGGGCCTAAG

Downstream 100 bases:

>100_bases
TGGTGGGGGTAAGACCATGGAGGAGTTAACGTATAAAGCTGCCGGGGTCGATATCGATGCCGGCATGGATGTGGTCCGGC
GCATTAAATCGGAAGTGGAG

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 452; Mature: 451

Protein sequence:

>452_residues
MGFEIREECGVFGIYAPGHDVARLTYWGLFALQHRGQESAGIAVGDGRDINLHKGMGLVAEVFSEEKLDGLKGFVAAGHV
RYSTTGASVPINAQPLVFHYKLGKIALGHNGNLANVLELKKNLLSRGAIFQTTTDSEVILNVIAQYAQTDLVEGILKAMV
DLKGAYSLVIMTENQLIGVRDPWGFRPLVLGRFYNGYVLASESAALDTIGAELIRDVEPGEIIVIDENGLKSYKFLQGMK
HSFCIFEFIYFARPDSIINGYHVNTVRRKMGQVLAKEYPVKADLVVPVPDSGTAAARGYAEASGIPLEEGLMKNRYAGRT
FIRPSQREREIGVKLKLNPIREVLKDKKIVVIDDSIVRGTTSKQIIELLRDAGAREIHLLLSSPPFIRSCYYGIDISRDD
ELIAAKYSVEEIKNYLGADGLYYLSLEGLLGIFKERDFCLACFTGDYPVPIN

Sequences:

>Translated_452_residues
MGFEIREECGVFGIYAPGHDVARLTYWGLFALQHRGQESAGIAVGDGRDINLHKGMGLVAEVFSEEKLDGLKGFVAAGHV
RYSTTGASVPINAQPLVFHYKLGKIALGHNGNLANVLELKKNLLSRGAIFQTTTDSEVILNVIAQYAQTDLVEGILKAMV
DLKGAYSLVIMTENQLIGVRDPWGFRPLVLGRFYNGYVLASESAALDTIGAELIRDVEPGEIIVIDENGLKSYKFLQGMK
HSFCIFEFIYFARPDSIINGYHVNTVRRKMGQVLAKEYPVKADLVVPVPDSGTAAARGYAEASGIPLEEGLMKNRYAGRT
FIRPSQREREIGVKLKLNPIREVLKDKKIVVIDDSIVRGTTSKQIIELLRDAGAREIHLLLSSPPFIRSCYYGIDISRDD
ELIAAKYSVEEIKNYLGADGLYYLSLEGLLGIFKERDFCLACFTGDYPVPIN
>Mature_451_residues
GFEIREECGVFGIYAPGHDVARLTYWGLFALQHRGQESAGIAVGDGRDINLHKGMGLVAEVFSEEKLDGLKGFVAAGHVR
YSTTGASVPINAQPLVFHYKLGKIALGHNGNLANVLELKKNLLSRGAIFQTTTDSEVILNVIAQYAQTDLVEGILKAMVD
LKGAYSLVIMTENQLIGVRDPWGFRPLVLGRFYNGYVLASESAALDTIGAELIRDVEPGEIIVIDENGLKSYKFLQGMKH
SFCIFEFIYFARPDSIINGYHVNTVRRKMGQVLAKEYPVKADLVVPVPDSGTAAARGYAEASGIPLEEGLMKNRYAGRTF
IRPSQREREIGVKLKLNPIREVLKDKKIVVIDDSIVRGTTSKQIIELLRDAGAREIHLLLSSPPFIRSCYYGIDISRDDE
LIAAKYSVEEIKNYLGADGLYYLSLEGLLGIFKERDFCLACFTGDYPVPIN

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=506, Percent_Identity=42.2924901185771, Blast_Score=365, Evalue=1e-101,
Organism=Escherichia coli, GI1788651, Length=467, Percent_Identity=39.186295503212, Blast_Score=294, Evalue=9e-81,
Organism=Escherichia coli, GI1790167, Length=204, Percent_Identity=32.843137254902, Blast_Score=82, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17554892, Length=479, Percent_Identity=40.9185803757829, Blast_Score=330, Evalue=8e-91,
Organism=Saccharomyces cerevisiae, GI6323958, Length=472, Percent_Identity=36.6525423728814, Blast_Score=266, Evalue=5e-72,
Organism=Drosophila melanogaster, GI28573187, Length=489, Percent_Identity=43.3537832310838, Blast_Score=381, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24659598, Length=483, Percent_Identity=42.8571428571429, Blast_Score=379, Evalue=1e-105,
Organism=Drosophila melanogaster, GI24659604, Length=458, Percent_Identity=43.2314410480349, Blast_Score=366, Evalue=1e-101,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 49946; Mature: 49815

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER ; PS00443 GATASE_TYPE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGFEIREECGVFGIYAPGHDVARLTYWGLFALQHRGQESAGIAVGDGRDINLHKGMGLVA
CCCCCCHHCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCEEECCCCEEEHHCCCHHHH
EVFSEEKLDGLKGFVAAGHVRYSTTGASVPINAQPLVFHYKLGKIALGHNGNLANVLELK
HHHHHHHHHHHHHHHEECEEEEECCCCCCCCCCCCEEEEEEECEEEECCCCCHHHHHHHH
KNLLSRGAIFQTTTDSEVILNVIAQYAQTDLVEGILKAMVDLKGAYSLVIMTENQLIGVR
HHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCEEECC
DPWGFRPLVLGRFYNGYVLASESAALDTIGAELIRDVEPGEIIVIDENGLKSYKFLQGMK
CCCCCCHHEEEEECCCEEEECCCCHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHCCC
HSFCIFEFIYFARPDSIINGYHVNTVRRKMGQVLAKEYPVKADLVVPVPDSGTAAARGYA
CHHHHHHHHHHCCCCCHHCCEEHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHCCCH
EASGIPLEEGLMKNRYAGRTFIRPSQREREIGVKLKLNPIREVLKDKKIVVIDDSIVRGT
HCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHCCCCEEEECCCHHCCC
TSKQIIELLRDAGAREIHLLLSSPPFIRSCYYGIDISRDDELIAAKYSVEEIKNYLGADG
CHHHHHHHHHHCCCCEEEEEECCCCHHHHHEECCEECCCCCEEEEECCHHHHHHHHCCCC
LYYLSLEGLLGIFKERDFCLACFTGDYPVPIN
EEEEEHHHHHHHHCCCCEEEEEEECCCCCCCC
>Mature Secondary Structure 
GFEIREECGVFGIYAPGHDVARLTYWGLFALQHRGQESAGIAVGDGRDINLHKGMGLVA
CCCCCHHCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCCEEECCCCEEEHHCCCHHHH
EVFSEEKLDGLKGFVAAGHVRYSTTGASVPINAQPLVFHYKLGKIALGHNGNLANVLELK
HHHHHHHHHHHHHHHEECEEEEECCCCCCCCCCCCEEEEEEECEEEECCCCCHHHHHHHH
KNLLSRGAIFQTTTDSEVILNVIAQYAQTDLVEGILKAMVDLKGAYSLVIMTENQLIGVR
HHHHHCCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCCEEECC
DPWGFRPLVLGRFYNGYVLASESAALDTIGAELIRDVEPGEIIVIDENGLKSYKFLQGMK
CCCCCCHHEEEEECCCEEEECCCCHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHCCC
HSFCIFEFIYFARPDSIINGYHVNTVRRKMGQVLAKEYPVKADLVVPVPDSGTAAARGYA
CHHHHHHHHHHCCCCCHHCCEEHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHCCCH
EASGIPLEEGLMKNRYAGRTFIRPSQREREIGVKLKLNPIREVLKDKKIVVIDDSIVRGT
HCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEHHHHHHHCCCCEEEECCCHHCCC
TSKQIIELLRDAGAREIHLLLSSPPFIRSCYYGIDISRDDELIAAKYSVEEIKNYLGADG
CHHHHHHHHHHCCCCEEEEEECCCCHHHHHEECCEECCCCCEEEEECCHHHHHHHHCCCC
LYYLSLEGLLGIFKERDFCLACFTGDYPVPIN
EEEEEHHHHHHHHCCCCEEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]