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The map label for this gene is hpt [H]

Identifier: 78042635

GI number: 78042635

Start: 943505

End: 944044

Strand: Direct

Name: hpt [H]

Synonym: CHY_1067

Alternate gene names: 78042635

Gene position: 943505-944044 (Clockwise)

Preceding gene: 78043218

Following gene: 78044956

Centisome position: 39.29

GC content: 43.52

Gene sequence:

>540_bases
TTGCATCAGGATATTGCGGAAATTTTAATATCCGAAGAAGAAATTAGGGCTAAAGTTAAAGAGCTTGGGCAACAAATTTC
CCGGGATTACGCCGGGCAGGATTTGCTGTTGGTTGGAATTTTACGCGGTGCAATGCTCTTTATGTCCGATTTAATGCGGG
AAATTGATATTCCGATAAACATCGATTTTATGGTGGTCTCCAGTTACGGTTCCGGTACCACCACCAGCGGCGAAGTGCGG
GTGTTAAAGGACCTTGACCGCGGGATTGAAGGAAGAAACGTTTTGTTAATTGAAGATATTGTTGATACCGGTCTTACTCT
AAATTACCTCACCAAGTACCTTGCCAACCGCCATCCGAAATCCCTTAAAGTCTGTACTTTATTAAACAAGCCAAGCCGCA
GGCGGGTTGAAGTAAAGGTGGATTATAACGGTTTTATCATTCCCGATAAATTTGTAGTAGGTTACGGCCTGGATTACAAC
GAGTATTACCGAAACCTTCCCTACATCGGCGTCTTAAAACCGGAGATTTACCGGGAATAA

Upstream 100 bases:

>100_bases
TCCCGGCTAACCTTGCAAAGAGTTTTTACAAGTTATTTCTTGACATTTGTAAATTATTTTTGGTATAATTTCCGCATCAA
AGTTTGGAGGAGGTATTACT

Downstream 100 bases:

>100_bases
TTTTAATTACACCTACAACCCACCACATACCACTTACCACTTTTAAAGGAGGTTTTTATGGCGTCAAAGGTAATTGTTTT
GGATTTTGGAGGACAGTACA

Product: hypoxanthine phosphoribosyltransferase

Products: NA

Alternate protein names: tRNA(Ile)-lysidine synthase; tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase; Hypoxanthine-guanine phosphoribosyltransferase; HGPRT; HGPRTase [H]

Number of amino acids: Translated: 179; Mature: 179

Protein sequence:

>179_residues
MHQDIAEILISEEEIRAKVKELGQQISRDYAGQDLLLVGILRGAMLFMSDLMREIDIPINIDFMVVSSYGSGTTTSGEVR
VLKDLDRGIEGRNVLLIEDIVDTGLTLNYLTKYLANRHPKSLKVCTLLNKPSRRRVEVKVDYNGFIIPDKFVVGYGLDYN
EYYRNLPYIGVLKPEIYRE

Sequences:

>Translated_179_residues
MHQDIAEILISEEEIRAKVKELGQQISRDYAGQDLLLVGILRGAMLFMSDLMREIDIPINIDFMVVSSYGSGTTTSGEVR
VLKDLDRGIEGRNVLLIEDIVDTGLTLNYLTKYLANRHPKSLKVCTLLNKPSRRRVEVKVDYNGFIIPDKFVVGYGLDYN
EYYRNLPYIGVLKPEIYRE
>Mature_179_residues
MHQDIAEILISEEEIRAKVKELGQQISRDYAGQDLLLVGILRGAMLFMSDLMREIDIPINIDFMVVSSYGSGTTTSGEVR
VLKDLDRGIEGRNVLLIEDIVDTGLTLNYLTKYLANRHPKSLKVCTLLNKPSRRRVEVKVDYNGFIIPDKFVVGYGLDYN
EYYRNLPYIGVLKPEIYRE

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0634

COG function: function code F; Hypoxanthine-guanine phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI4504483, Length=180, Percent_Identity=35.5555555555556, Blast_Score=115, Evalue=2e-26,
Organism=Homo sapiens, GI9910262, Length=181, Percent_Identity=32.5966850828729, Blast_Score=100, Evalue=1e-21,
Organism=Escherichia coli, GI87081689, Length=166, Percent_Identity=54.8192771084337, Blast_Score=198, Evalue=2e-52,
Organism=Caenorhabditis elegans, GI17510817, Length=180, Percent_Identity=34.4444444444444, Blast_Score=103, Evalue=5e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005904
- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR020825
- InterPro:   IPR000836
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF00156 Pribosyltran; PF11734 TilS_C [H]

EC number: =2.4.2.8 [H]

Molecular weight: Translated: 20476; Mature: 20476

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHQDIAEILISEEEIRAKVKELGQQISRDYAGQDLLLVGILRGAMLFMSDLMREIDIPIN
CCHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
IDFMVVSSYGSGTTTSGEVRVLKDLDRGIEGRNVLLIEDIVDTGLTLNYLTKYLANRHPK
EEEEEEEECCCCCCCCCCEEEHHHHHCCCCCCCEEEEEHHHHCCCCHHHHHHHHHCCCCC
SLKVCTLLNKPSRRRVEVKVDYNGFIIPDKFVVGYGLDYNEYYRNLPYIGVLKPEIYRE
CEEEEEECCCCCCCEEEEEECCCCEECCCCEEEEECCCHHHHHHCCCEEEEECHHHHCC
>Mature Secondary Structure
MHQDIAEILISEEEIRAKVKELGQQISRDYAGQDLLLVGILRGAMLFMSDLMREIDIPIN
CCHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCC
IDFMVVSSYGSGTTTSGEVRVLKDLDRGIEGRNVLLIEDIVDTGLTLNYLTKYLANRHPK
EEEEEEEECCCCCCCCCCEEEHHHHHCCCCCCCEEEEEHHHHCCCCHHHHHHHHHCCCCC
SLKVCTLLNKPSRRRVEVKVDYNGFIIPDKFVVGYGLDYNEYYRNLPYIGVLKPEIYRE
CEEEEEECCCCCCCEEEEEECCCCEECCCCEEEEECCCHHHHHHCCCEEEEECHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11679669 [H]