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The map label for this gene is cfaA [H]

Identifier: 77748582

GI number: 77748582

Start: 1582985

End: 1584280

Strand: Reverse

Name: cfaA [H]

Synonym: XAC1375

Alternate gene names: 77748582

Gene position: 1584280-1582985 (Counterclockwise)

Preceding gene: 21242129

Following gene: 21242127

Centisome position: 30.61

GC content: 65.12

Gene sequence:

>1296_bases
ATGAACGCCACGTCCCTGCCCGATCACGCTGCGCCGCGCGCGGCCTCCTGGACGGACCGTCTGCTGCGCAAGCGCCTGCT
GGCCACGCTTGGGCAACTGCGCGATGGACAGCTGCAGCTGCATGAAGCCGGTACTGTCGCCACGCTTGGCACGGCCACTG
GCGCGCAGGCCTTGCAGATGCAGTTGCGCGTGATCGACCCCGGTTTTTATCGGCAGGCGGCGCTCAACGGCAGCGTCGGT
GCGGGCGAGTCGTATATGGATGGGCAGTGGCAGTGCGACGACCTGGTGGCGTTGATGCGGCTGCTGCTGCGCAACCGCGA
GCGGCTGGACGCGATGGAGACCGGCACCGCGCGCCTGGGCGGCTTGGCCATGCGCAGCCTGCATGCGTTTGCGCGCAATA
CCCGCAGCGGCAGCCGGCGCAATATCGCCGCGCATTACGACCTGGGCAACCCGCTGTTCAAACTGTTTCTGGATGAAAAC
CTGATGTACTCGTCGGCCATCTTTGTCGACGGCGAAGAGGCGCAGGGCCAAGCGGCACTGGAGCGCGCGGCGACCCGCAA
GCTCGAACGCATCTGCCAGAAACTGCGCCTGGCGCCGCACCATCATGTGGTGGAGATCGGCACCGGCTGGGGCGGCTTTG
CACTGCACGCCGCACGCGAACACGGCTGCCGCGTCACCACCACCACCATTTCCCGCGAGCAGTTCGAGCTGGCCAACCAA
CGCATCGCCGCGGCAGGTCTGAGCGATCGGGTGACGGTGCTGTTGCGCGACTACCGCGACCTGGAAGGCCGCTTCGACCG
CGTGGTCTCGATCGAGATGATCGAAGCCATCGGCCACCAGTATCTGGATACGTACTTCGCCAAGGTCGGCAGCCTGCTGG
CTGACGATGGCGAAGCGCTGATCCAGGCCATCACCATCGAAGACCACCGCTACAAACAGGCGCTGCATTCGGTGGACTTC
ATCAAACGGCATATCTTCCCTGGCAGCTTCATCCCGTCGGTCGCGGCGATGACCGGCGCAGTCGCGCGCGCCAGCGATCT
GCGCCTGTTCAACCTGGAAGACATCGGCCCGAGCTATGCGTTGACCTTGCGTGCGTGGCGGCAGCGTTTCATGGCGCAGT
TGCCGCAGGTGCGTGCGCTGGGTTACGACGAGCGCTTCATCCGCATGTGGGAGTTTTACCTGGCCTATTGCGAGGCCGGT
TTTCTGGAACGCTCGATCGGCGATGTGCATTTGTGGCTAAACAAGCCCGGTGCACGCCCGCCGCAATTCGTGCCGGGCCT
CACCGCCGACGCATGA

Upstream 100 bases:

>100_bases
CGGTGTGGGTGGTGATCGCCATCCACTGGCAGGCCTTGCGCCTGTGGCTGCGCGGCAACCCGGTCCACGACCACCCTCAC
CCGCCTGTGCGAGAACCCCG

Downstream 100 bases:

>100_bases
AGCAGCTCGGCAACTACCTCGGCCTGCAGGTGCTGTGGCTGATGGCGGTCAGCTCCGCTGCGCATGCGCGCATCTGGCCA
ACGCTGCTGGCGCTGGCCGT

Product: cyclopropane-fatty-acyl-phospholipid synthase

Products: NA

Alternate protein names: CFA synthase; Cyclopropane fatty acid synthase [H]

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MNATSLPDHAAPRAASWTDRLLRKRLLATLGQLRDGQLQLHEAGTVATLGTATGAQALQMQLRVIDPGFYRQAALNGSVG
AGESYMDGQWQCDDLVALMRLLLRNRERLDAMETGTARLGGLAMRSLHAFARNTRSGSRRNIAAHYDLGNPLFKLFLDEN
LMYSSAIFVDGEEAQGQAALERAATRKLERICQKLRLAPHHHVVEIGTGWGGFALHAAREHGCRVTTTTISREQFELANQ
RIAAAGLSDRVTVLLRDYRDLEGRFDRVVSIEMIEAIGHQYLDTYFAKVGSLLADDGEALIQAITIEDHRYKQALHSVDF
IKRHIFPGSFIPSVAAMTGAVARASDLRLFNLEDIGPSYALTLRAWRQRFMAQLPQVRALGYDERFIRMWEFYLAYCEAG
FLERSIGDVHLWLNKPGARPPQFVPGLTADA

Sequences:

>Translated_431_residues
MNATSLPDHAAPRAASWTDRLLRKRLLATLGQLRDGQLQLHEAGTVATLGTATGAQALQMQLRVIDPGFYRQAALNGSVG
AGESYMDGQWQCDDLVALMRLLLRNRERLDAMETGTARLGGLAMRSLHAFARNTRSGSRRNIAAHYDLGNPLFKLFLDEN
LMYSSAIFVDGEEAQGQAALERAATRKLERICQKLRLAPHHHVVEIGTGWGGFALHAAREHGCRVTTTTISREQFELANQ
RIAAAGLSDRVTVLLRDYRDLEGRFDRVVSIEMIEAIGHQYLDTYFAKVGSLLADDGEALIQAITIEDHRYKQALHSVDF
IKRHIFPGSFIPSVAAMTGAVARASDLRLFNLEDIGPSYALTLRAWRQRFMAQLPQVRALGYDERFIRMWEFYLAYCEAG
FLERSIGDVHLWLNKPGARPPQFVPGLTADA
>Mature_431_residues
MNATSLPDHAAPRAASWTDRLLRKRLLATLGQLRDGQLQLHEAGTVATLGTATGAQALQMQLRVIDPGFYRQAALNGSVG
AGESYMDGQWQCDDLVALMRLLLRNRERLDAMETGTARLGGLAMRSLHAFARNTRSGSRRNIAAHYDLGNPLFKLFLDEN
LMYSSAIFVDGEEAQGQAALERAATRKLERICQKLRLAPHHHVVEIGTGWGGFALHAAREHGCRVTTTTISREQFELANQ
RIAAAGLSDRVTVLLRDYRDLEGRFDRVVSIEMIEAIGHQYLDTYFAKVGSLLADDGEALIQAITIEDHRYKQALHSVDF
IKRHIFPGSFIPSVAAMTGAVARASDLRLFNLEDIGPSYALTLRAWRQRFMAQLPQVRALGYDERFIRMWEFYLAYCEAG
FLERSIGDVHLWLNKPGARPPQFVPGLTADA

Specific function: Transfers a methylene group from S-adenosyl-L-methionine to the cis double bond of an unsaturated fatty acid chain resulting in the replacement of the double bond with a methylene bridge [H]

COG id: COG2230

COG function: function code M; Cyclopropane fatty acid synthase and related methyltransferases

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CFA/CMAS family [H]

Homologues:

Organism=Escherichia coli, GI1787951, Length=356, Percent_Identity=38.4831460674157, Blast_Score=210, Evalue=1e-55,
Organism=Caenorhabditis elegans, GI133904226, Length=408, Percent_Identity=34.3137254901961, Blast_Score=238, Evalue=5e-63,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003333 [H]

Pfam domain/function: PF02353 CMAS [H]

EC number: =2.1.1.79 [H]

Molecular weight: Translated: 48046; Mature: 48046

Theoretical pI: Translated: 8.33; Mature: 8.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNATSLPDHAAPRAASWTDRLLRKRLLATLGQLRDGQLQLHEAGTVATLGTATGAQALQM
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCEEEECCCCCHHHHHH
QLRVIDPGFYRQAALNGSVGAGESYMDGQWQCDDLVALMRLLLRNRERLDAMETGTARLG
HHEEECCCHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHCCCHHHH
GLAMRSLHAFARNTRSGSRRNIAAHYDLGNPLFKLFLDENLMYSSAIFVDGEEAQGQAAL
HHHHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHCCCCEEEEEEEEECCCCCCHHHH
ERAATRKLERICQKLRLAPHHHVVEIGTGWGGFALHAAREHGCRVTTTTISREQFELANQ
HHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHCCCEEEEEECCHHHHHHHHH
RIAAAGLSDRVTVLLRDYRDLEGRFDRVVSIEMIEAIGHQYLDTYFAKVGSLLADDGEAL
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
IQAITIEDHRYKQALHSVDFIKRHIFPGSFIPSVAAMTGAVARASDLRLFNLEDIGPSYA
HEEEECCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCEEEEHHHCCCHHH
LTLRAWRQRFMAQLPQVRALGYDERFIRMWEFYLAYCEAGFLERSIGDVHLWLNKPGARP
HHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCC
PQFVPGLTADA
CCCCCCCCCCC
>Mature Secondary Structure
MNATSLPDHAAPRAASWTDRLLRKRLLATLGQLRDGQLQLHEAGTVATLGTATGAQALQM
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCCEEEECCCCCHHHHHH
QLRVIDPGFYRQAALNGSVGAGESYMDGQWQCDDLVALMRLLLRNRERLDAMETGTARLG
HHEEECCCHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHCCCHHHH
GLAMRSLHAFARNTRSGSRRNIAAHYDLGNPLFKLFLDENLMYSSAIFVDGEEAQGQAAL
HHHHHHHHHHHHCCCCCCCCCEEEEECCCCHHHHHHHCCCCEEEEEEEEECCCCCCHHHH
ERAATRKLERICQKLRLAPHHHVVEIGTGWGGFALHAAREHGCRVTTTTISREQFELANQ
HHHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHCCCEEEEEECCHHHHHHHHH
RIAAAGLSDRVTVLLRDYRDLEGRFDRVVSIEMIEAIGHQYLDTYFAKVGSLLADDGEAL
HHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHH
IQAITIEDHRYKQALHSVDFIKRHIFPGSFIPSVAAMTGAVARASDLRLFNLEDIGPSYA
HEEEECCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCEEEEHHHCCCHHH
LTLRAWRQRFMAQLPQVRALGYDERFIRMWEFYLAYCEAGFLERSIGDVHLWLNKPGARP
HHHHHHHHHHHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCC
PQFVPGLTADA
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]