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The map label for this gene is potB [H]

Identifier: 77465396

GI number: 77465396

Start: 459079

End: 459951

Strand: Reverse

Name: potB [H]

Synonym: RSP_3395

Alternate gene names: 77465396

Gene position: 459951-459079 (Counterclockwise)

Preceding gene: 77465397

Following gene: 77465395

Centisome position: 48.77

GC content: 67.93

Gene sequence:

>873_bases
ATGACGGCCGTTCCCGCGGTCGCGGCCGGCCGGCGGCCGATGGGCGGGCAGCAGGTGGCGGTGGCGCTCCTCCTGCCCAT
CGCCCTCATCAATGTGGTGGGCTTCATGCTGCCCATCTTCAACCTCGCCCGCTATTCCTTCAACCGGGCGACGGTGGGCG
GGGGGATCGAGAACGTCCTGACGCTCCAGAACTGGACCGGGCTTCTCAGCGACCCGTTCTATCTGCAGCTGATCCTGAAC
TCGGTCTTCGTCTCGCTCGGGGTCACGCTCTGTGCACTCGTCCTGTCCTATCCGATCGCGCTCTATCTCCACCGCGCCTC
GGGCAGCTGGCGGACCTTCCTCACCGTGCTCGTGATCTCGCCGCTCCTGACCTCGGCGGTGGTGCGCACCTACGGCTGGA
TCGCGATCCTCGCCGAGGAGGGGCCGGTCGTCTCGGCGATCCGGGGCCTGGGCCTCACGCCGCCGCCGCTCATGTTCAAC
ATCAACGGCGTCTTCATCGGCCTGACCGAGATCCTGATGCCCTATATGATCCTCGCCCTTCTCGCGGGGTTCGGGCGGCT
CGATCCGCGCGTCGAGGAGGCCGCGCGCACGCTCGGCGCCACCCCCTGGCAGAGCTTCCGGCGCGTGATCCTGCCGCTGA
CCGCGCCCGGCATCGCGCTGGGGTGCCTCCTGTGCTTCGTGCTGGCGGTCTCGTCCTTCATCACGCCGAAGCTCATGGGG
GGCGGGCGCGTCTTCCTGCTCGCGACCGAGATCTACGATCAGGCCATCGTGACCCTGAACTGGCCGCTGGCCTCGACGCT
CTCGATCCTCGTCCTCGTGATCTTCGGGGCGGCGCTCGTGGCCTATTCCGCCGTGCTGAAACGTCTGGACTGA

Upstream 100 bases:

>100_bases
AGCAGCTCACGCGCCGCCGCTCGGGCCTGCCCGAGCCGGGCTCGGCCGTGACCGTCGCCTGGGAGGCCGGCGACACGATG
GTCTATCCCGAGGGCAGGGC

Downstream 100 bases:

>100_bases
GGAGGGCGCCATGCAGATCCACAAGACCTCGAAGCCGATGGGGATCCTCGTCGCGGTGCTCTTCCTCTATCTGCTCGCGC
CGGTGATCCTCGTCTTCCCG

Product: ABC opine/polyamine transporter, inner membrane subunit

Products: phosphate; putrescine [Cytoplasm]; ADP; spermidine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 290; Mature: 289

Protein sequence:

>290_residues
MTAVPAVAAGRRPMGGQQVAVALLLPIALINVVGFMLPIFNLARYSFNRATVGGGIENVLTLQNWTGLLSDPFYLQLILN
SVFVSLGVTLCALVLSYPIALYLHRASGSWRTFLTVLVISPLLTSAVVRTYGWIAILAEEGPVVSAIRGLGLTPPPLMFN
INGVFIGLTEILMPYMILALLAGFGRLDPRVEEAARTLGATPWQSFRRVILPLTAPGIALGCLLCFVLAVSSFITPKLMG
GGRVFLLATEIYDQAIVTLNWPLASTLSILVLVIFGAALVAYSAVLKRLD

Sequences:

>Translated_290_residues
MTAVPAVAAGRRPMGGQQVAVALLLPIALINVVGFMLPIFNLARYSFNRATVGGGIENVLTLQNWTGLLSDPFYLQLILN
SVFVSLGVTLCALVLSYPIALYLHRASGSWRTFLTVLVISPLLTSAVVRTYGWIAILAEEGPVVSAIRGLGLTPPPLMFN
INGVFIGLTEILMPYMILALLAGFGRLDPRVEEAARTLGATPWQSFRRVILPLTAPGIALGCLLCFVLAVSSFITPKLMG
GGRVFLLATEIYDQAIVTLNWPLASTLSILVLVIFGAALVAYSAVLKRLD
>Mature_289_residues
TAVPAVAAGRRPMGGQQVAVALLLPIALINVVGFMLPIFNLARYSFNRATVGGGIENVLTLQNWTGLLSDPFYLQLILNS
VFVSLGVTLCALVLSYPIALYLHRASGSWRTFLTVLVISPLLTSAVVRTYGWIAILAEEGPVVSAIRGLGLTPPPLMFNI
NGVFIGLTEILMPYMILALLAGFGRLDPRVEEAARTLGATPWQSFRRVILPLTAPGIALGCLLCFVLAVSSFITPKLMGG
GRVFLLATEIYDQAIVTLNWPLASTLSILVLVIFGAALVAYSAVLKRLD

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine and spermidine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787369, Length=239, Percent_Identity=37.2384937238494, Blast_Score=142, Evalue=2e-35,
Organism=Escherichia coli, GI1787080, Length=224, Percent_Identity=33.9285714285714, Blast_Score=124, Evalue=8e-30,
Organism=Escherichia coli, GI1787713, Length=259, Percent_Identity=31.6602316602317, Blast_Score=112, Evalue=3e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 31053; Mature: 30922

Theoretical pI: Translated: 9.66; Mature: 9.66

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAVPAVAAGRRPMGGQQVAVALLLPIALINVVGFMLPIFNLARYSFNRATVGGGIENVL
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
TLQNWTGLLSDPFYLQLILNSVFVSLGVTLCALVLSYPIALYLHRASGSWRTFLTVLVIS
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHH
PLLTSAVVRTYGWIAILAEEGPVVSAIRGLGLTPPPLMFNINGVFIGLTEILMPYMILAL
HHHHHHHHHHHHHEEEEECCCCHHHHHHHCCCCCCCCEEECCHHHHHHHHHHHHHHHHHH
LAGFGRLDPRVEEAARTLGATPWQSFRRVILPLTAPGIALGCLLCFVLAVSSFITPKLMG
HHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHCC
GGRVFLLATEIYDQAIVTLNWPLASTLSILVLVIFGAALVAYSAVLKRLD
CCEEEEEEHHHHHCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TAVPAVAAGRRPMGGQQVAVALLLPIALINVVGFMLPIFNLARYSFNRATVGGGIENVL
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHH
TLQNWTGLLSDPFYLQLILNSVFVSLGVTLCALVLSYPIALYLHRASGSWRTFLTVLVIS
HHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEECCCCCHHHHHHHHHHH
PLLTSAVVRTYGWIAILAEEGPVVSAIRGLGLTPPPLMFNINGVFIGLTEILMPYMILAL
HHHHHHHHHHHHHEEEEECCCCHHHHHHHCCCCCCCCEEECCHHHHHHHHHHHHHHHHHH
LAGFGRLDPRVEEAARTLGATPWQSFRRVILPLTAPGIALGCLLCFVLAVSSFITPKLMG
HHCCCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCHHHCC
GGRVFLLATEIYDQAIVTLNWPLASTLSILVLVIFGAALVAYSAVLKRLD
CCEEEEEEHHHHHCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: putrescine [Periplasm]; H2O; ATP; spermidine [Periplasm] [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] ATP + spermidine [Periplasm] + H2O = ADP + phosphate + spermidine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]