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The map label for this gene is potI [H]

Identifier: 77465395

GI number: 77465395

Start: 458274

End: 459068

Strand: Reverse

Name: potI [H]

Synonym: RSP_3393

Alternate gene names: 77465395

Gene position: 459068-458274 (Counterclockwise)

Preceding gene: 77465396

Following gene: 77465394

Centisome position: 48.68

GC content: 66.67

Gene sequence:

>795_bases
ATGCAGATCCACAAGACCTCGAAGCCGATGGGGATCCTCGTCGCGGTGCTCTTCCTCTATCTGCTCGCGCCGGTGATCCT
CGTCTTCCCGATCTCCCTCTCGGGCGACAGCGTGCTGCGCTTTCCGCCGACCTCCTGGTCCCTCCGCTGGTACGAGGCCC
TGTTCGAGAACCGAGCCATGCTCGGCGCCTTCTGGACGAGCCTGTCGCTCGCGGCCATCGTCACCGTGCTCTCGCTGGTG
ATCGCGGTGCCGGCGGCCTGGGTGATGGTGCGGATGGAGTTCCGCGGGCGGGATCTCCTGTTCAACCTCTTCACCGCGCC
CCTCCTGCTGCCCACCATCGTGCTGGGGCTCGCCATCCTCATCGTCTTCGCCTCCTTCGGGCTTCTGGCGACGATGCAGG
GGCTGATCCTCGCCCATCTCGTCATCACGCTGCCCTATGCGCTGCGCATTCTGGCCGTTTCGCTCGCCACGCAGAACATG
GCCTGCGAGGAGGCCGCAAAGACCCTCGGCGCGAAGCCCCTCACCGTCTTCTTCCGGGTGACGCTGCCGATGCTCACCCC
GGGGATCGTGGCGGCGGCGGCGCTCTGCTTCCTCGTCTCCTTCGACGAGGTGGTGATCACGCTCTTCCTCACCGGGCCGC
GCATCAGCACGCTGCCGGTCGAGCTCTACAACTACGTCTACAACACCGCCGATCCGCTGGTGGCCGCGGCCTCCGTGCTG
CTGATCCTGCTGACGCTGGGCGTGATCCTCGTGGTCGAGCGCGCGATGGGCCTCGGGCGGGCCTTCGTGAAGTAA

Upstream 100 bases:

>100_bases
CCGCTGGCCTCGACGCTCTCGATCCTCGTCCTCGTGATCTTCGGGGCGGCGCTCGTGGCCTATTCCGCCGTGCTGAAACG
TCTGGACTGAGGAGGGCGCC

Downstream 100 bases:

>100_bases
ACCGCACCCGCGCGACCTTCGGGGCCGGTTGTTCCGGGGGAACGGCCGGCCCCGTTCTCATCGGCGCGCGCCCTCTCCCC
TTGTCAACCGCGGCGCGACC

Product: ABC opine/polyamine transporter, inner membrane subunit

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 264; Mature: 264

Protein sequence:

>264_residues
MQIHKTSKPMGILVAVLFLYLLAPVILVFPISLSGDSVLRFPPTSWSLRWYEALFENRAMLGAFWTSLSLAAIVTVLSLV
IAVPAAWVMVRMEFRGRDLLFNLFTAPLLLPTIVLGLAILIVFASFGLLATMQGLILAHLVITLPYALRILAVSLATQNM
ACEEAAKTLGAKPLTVFFRVTLPMLTPGIVAAAALCFLVSFDEVVITLFLTGPRISTLPVELYNYVYNTADPLVAAASVL
LILLTLGVILVVERAMGLGRAFVK

Sequences:

>Translated_264_residues
MQIHKTSKPMGILVAVLFLYLLAPVILVFPISLSGDSVLRFPPTSWSLRWYEALFENRAMLGAFWTSLSLAAIVTVLSLV
IAVPAAWVMVRMEFRGRDLLFNLFTAPLLLPTIVLGLAILIVFASFGLLATMQGLILAHLVITLPYALRILAVSLATQNM
ACEEAAKTLGAKPLTVFFRVTLPMLTPGIVAAAALCFLVSFDEVVITLFLTGPRISTLPVELYNYVYNTADPLVAAASVL
LILLTLGVILVVERAMGLGRAFVK
>Mature_264_residues
MQIHKTSKPMGILVAVLFLYLLAPVILVFPISLSGDSVLRFPPTSWSLRWYEALFENRAMLGAFWTSLSLAAIVTVLSLV
IAVPAAWVMVRMEFRGRDLLFNLFTAPLLLPTIVLGLAILIVFASFGLLATMQGLILAHLVITLPYALRILAVSLATQNM
ACEEAAKTLGAKPLTVFFRVTLPMLTPGIVAAAALCFLVSFDEVVITLFLTGPRISTLPVELYNYVYNTADPLVAAASVL
LILLTLGVILVVERAMGLGRAFVK

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1177

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component II

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787081, Length=243, Percent_Identity=32.9218106995885, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1787368, Length=198, Percent_Identity=29.2929292929293, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1787714, Length=233, Percent_Identity=29.1845493562232, Blast_Score=82, Evalue=2e-17,
Organism=Escherichia coli, GI1788764, Length=187, Percent_Identity=29.9465240641711, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI87082099, Length=195, Percent_Identity=29.7435897435897, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1786254, Length=230, Percent_Identity=28.2608695652174, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1786980, Length=199, Percent_Identity=29.1457286432161, Blast_Score=64, Evalue=8e-12,
Organism=Escherichia coli, GI1787571, Length=192, Percent_Identity=26.5625, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1787080, Length=210, Percent_Identity=27.1428571428571, Blast_Score=61, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 28647; Mature: 28647

Theoretical pI: Translated: 9.45; Mature: 9.45

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQIHKTSKPMGILVAVLFLYLLAPVILVFPISLSGDSVLRFPPTSWSLRWYEALFENRAM
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHCCHH
LGAFWTSLSLAAIVTVLSLVIAVPAAWVMVRMEFRGRDLLFNLFTAPLLLPTIVLGLAIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
IVFASFGLLATMQGLILAHLVITLPYALRILAVSLATQNMACEEAAKTLGAKPLTVFFRV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
TLPMLTPGIVAAAALCFLVSFDEVVITLFLTGPRISTLPVELYNYVYNTADPLVAAASVL
HHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHH
LILLTLGVILVVERAMGLGRAFVK
HHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQIHKTSKPMGILVAVLFLYLLAPVILVFPISLSGDSVLRFPPTSWSLRWYEALFENRAM
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHCCHH
LGAFWTSLSLAAIVTVLSLVIAVPAAWVMVRMEFRGRDLLFNLFTAPLLLPTIVLGLAIL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
IVFASFGLLATMQGLILAHLVITLPYALRILAVSLATQNMACEEAAKTLGAKPLTVFFRV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
TLPMLTPGIVAAAALCFLVSFDEVVITLFLTGPRISTLPVELYNYVYNTADPLVAAASVL
HHHHHCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHH
LILLTLGVILVVERAMGLGRAFVK
HHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]