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The map label for this gene is 77465371

Identifier: 77465371

GI number: 77465371

Start: 432872

End: 434230

Strand: Reverse

Name: 77465371

Synonym: RSP_3369

Alternate gene names: NA

Gene position: 434230-432872 (Counterclockwise)

Preceding gene: 77465372

Following gene: 77465370

Centisome position: 46.05

GC content: 76.09

Gene sequence:

>1359_bases
ATGGGGCGCGCTGATCGCAACGGAGGGCTGCTCGCGCTGACCGGGGCCGAGCTTGCGGAGCGGCTCGCCTCGGGCGCGGT
GCGCGCGGTGGAACTGGTCGAGGCCTGCCTCGCGCAGATCGCCGCGCGCGAGCCGGAGGTGCAGGCCTGGGCCTTCCTCG
ACGGGGATCATGCGATGGCGCAGGCGCGCGCGCTCGATGCGCGCCGGCAGAGCGGGGCGGCGCTCGGCCCACTGCACGGA
CTGCCGGTGGGGGTGAAGGACGTGATCGACGTGCGCGGCATGCCGACCGGCCTCGGCCTTGCCGGCGGAGCGGGGCGGGT
GGCGCAGGCCGATGCCTTCGTCGCAGCCCGGCTGCGGGCGGCGGGGGCGATCCTGATGGGCAAGACCGCGACGGCCGAGG
CGGCCTTCCTCGAGCCGCCCGCCACGCGCAACCCGCACGACCCCGCCCGCACGCCGGGCGGCTCCTCCTCGGGCTCTGCG
GCGGCGGTGGCGGCGGGCATGGTGCCGCTGGCTATCGGCACCCAGACCGGCGGATCGGTGATCCGCCCCGCGGCCTTCTG
CGGCATCGCGGGCTTCAAGCCGAGCTTCGGCCTCATCCCCCGCTCGGGCGTCCTGACCCAGTCGCCGAGCCTCGACACGA
TGGGCGTCTTCGGCCGCAGCGTGGCCGACGTGGCGCTCCTGGCCGAGGCGCTCGCCGGCCACGATCCGGCCGATCCGGCG
AGCCTGCTGCAGCCCGCGCCGCGCCTGCCCGAGACCGCGGCCAGCGATCCGCCGGTGACGCCGACGCTGGCCTTCGTGCG
GCCGCCCGGCTGGGAGGCGGCGCATGAGGAGATGCGGCTCGCCCTGCAGGAACTGGCGGAGCTGCTGGGGCGGGACTGCC
TCGCGCTCGAGCTGCCCGCGGTCTTCGGCGCAGCGGCCGACCTGCGCCGCCGGATCAACCTGGCCGAGATGGCGCATCAC
TATGCGGGTCTTGCAGGCCGGGCGGCCCTGTCGCCCCTCCTGAGCGAGGCGCTGGCCGAGGGGCGGAGGATCCCGGCCGC
GGATTATCTCGCGGCGCTCGAGGCGCGGACGGCGCTGCGCGCAGGGCTGGAGCCGATCTTTCATCGCTGCGACGCGATCC
TCTGCCCTGCCGCGCCGGGCCCCGCGCCCGACCGCGCCACGACCGGAGACGCGATCTTCAACGGGCTCTGGACCTTCTGC
GGCCTGCCCGCCGTCACTCTGCCGCTCTTCGTCTCGTCCGACGGCCTGCCGATGGGGGCGCAGCTCGTGGGCCCGCCGGG
CGGCGACGCCCGCCTTCTGCGCACCGCCCGCTGGCTGGTCCAGCGCCTCGCTCAAACCGCACCGGAGACAGCCCCATGA

Upstream 100 bases:

>100_bases
ATCTTCGCGGGCTCCTTGCCCTATGTGGGGATGGTCTTCGTCTGCATGGCGCTGATGTATCTCTTCCCGCAGATCGTCTT
CGCACTTCCGGAGTTCGTCT

Downstream 100 bases:

>100_bases
CCCGTCGCCTGCTGCCCCTTTTCGCGCTGATCGTGCTCGCGGCCTTCCTCGGCATCCTTGCGATGAAGGTTCCGCGCCTC
GATCTGGGCCTCGTCGTGCT

Product: Asp-tRNA Asn/Glu-tRNA Gln amidotransferase subunit A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 452; Mature: 451

Protein sequence:

>452_residues
MGRADRNGGLLALTGAELAERLASGAVRAVELVEACLAQIAAREPEVQAWAFLDGDHAMAQARALDARRQSGAALGPLHG
LPVGVKDVIDVRGMPTGLGLAGGAGRVAQADAFVAARLRAAGAILMGKTATAEAAFLEPPATRNPHDPARTPGGSSSGSA
AAVAAGMVPLAIGTQTGGSVIRPAAFCGIAGFKPSFGLIPRSGVLTQSPSLDTMGVFGRSVADVALLAEALAGHDPADPA
SLLQPAPRLPETAASDPPVTPTLAFVRPPGWEAAHEEMRLALQELAELLGRDCLALELPAVFGAAADLRRRINLAEMAHH
YAGLAGRAALSPLLSEALAEGRRIPAADYLAALEARTALRAGLEPIFHRCDAILCPAAPGPAPDRATTGDAIFNGLWTFC
GLPAVTLPLFVSSDGLPMGAQLVGPPGGDARLLRTARWLVQRLAQTAPETAP

Sequences:

>Translated_452_residues
MGRADRNGGLLALTGAELAERLASGAVRAVELVEACLAQIAAREPEVQAWAFLDGDHAMAQARALDARRQSGAALGPLHG
LPVGVKDVIDVRGMPTGLGLAGGAGRVAQADAFVAARLRAAGAILMGKTATAEAAFLEPPATRNPHDPARTPGGSSSGSA
AAVAAGMVPLAIGTQTGGSVIRPAAFCGIAGFKPSFGLIPRSGVLTQSPSLDTMGVFGRSVADVALLAEALAGHDPADPA
SLLQPAPRLPETAASDPPVTPTLAFVRPPGWEAAHEEMRLALQELAELLGRDCLALELPAVFGAAADLRRRINLAEMAHH
YAGLAGRAALSPLLSEALAEGRRIPAADYLAALEARTALRAGLEPIFHRCDAILCPAAPGPAPDRATTGDAIFNGLWTFC
GLPAVTLPLFVSSDGLPMGAQLVGPPGGDARLLRTARWLVQRLAQTAPETAP
>Mature_451_residues
GRADRNGGLLALTGAELAERLASGAVRAVELVEACLAQIAAREPEVQAWAFLDGDHAMAQARALDARRQSGAALGPLHGL
PVGVKDVIDVRGMPTGLGLAGGAGRVAQADAFVAARLRAAGAILMGKTATAEAAFLEPPATRNPHDPARTPGGSSSGSAA
AVAAGMVPLAIGTQTGGSVIRPAAFCGIAGFKPSFGLIPRSGVLTQSPSLDTMGVFGRSVADVALLAEALAGHDPADPAS
LLQPAPRLPETAASDPPVTPTLAFVRPPGWEAAHEEMRLALQELAELLGRDCLALELPAVFGAAADLRRRINLAEMAHHY
AGLAGRAALSPLLSEALAEGRRIPAADYLAALEARTALRAGLEPIFHRCDAILCPAAPGPAPDRATTGDAIFNGLWTFCG
LPAVTLPLFVSSDGLPMGAQLVGPPGGDARLLRTARWLVQRLAQTAPETAP

Specific function: Unknown

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=276, Percent_Identity=32.6086956521739, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI195972892, Length=207, Percent_Identity=32.8502415458937, Blast_Score=106, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17543272, Length=408, Percent_Identity=28.1862745098039, Blast_Score=110, Evalue=2e-24,
Organism=Caenorhabditis elegans, GI17537465, Length=481, Percent_Identity=23.9085239085239, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI71990152, Length=254, Percent_Identity=29.5275590551181, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17538252, Length=199, Percent_Identity=31.6582914572864, Blast_Score=78, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17556264, Length=196, Percent_Identity=32.1428571428571, Blast_Score=72, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6319685, Length=454, Percent_Identity=26.2114537444934, Blast_Score=94, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6323950, Length=424, Percent_Identity=26.6509433962264, Blast_Score=86, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24648113, Length=466, Percent_Identity=29.8283261802575, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24648435, Length=481, Percent_Identity=24.7401247401247, Blast_Score=101, Evalue=9e-22,
Organism=Drosophila melanogaster, GI24648437, Length=481, Percent_Identity=24.7401247401247, Blast_Score=101, Evalue=9e-22,
Organism=Drosophila melanogaster, GI45550774, Length=481, Percent_Identity=24.7401247401247, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI161078093, Length=489, Percent_Identity=26.5848670756646, Blast_Score=98, Evalue=1e-20,
Organism=Drosophila melanogaster, GI21356731, Length=455, Percent_Identity=25.0549450549451, Blast_Score=94, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24648441, Length=415, Percent_Identity=25.3012048192771, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24648439, Length=415, Percent_Identity=25.3012048192771, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24652985, Length=483, Percent_Identity=24.4306418219462, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI19922090, Length=483, Percent_Identity=24.4306418219462, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24652981, Length=483, Percent_Identity=24.4306418219462, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24652983, Length=483, Percent_Identity=24.4306418219462, Blast_Score=82, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24644968, Length=258, Percent_Identity=27.1317829457364, Blast_Score=79, Evalue=8e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: =3.5.1.4 [H]

Molecular weight: Translated: 46129; Mature: 45998

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRADRNGGLLALTGAELAERLASGAVRAVELVEACLAQIAAREPEVQAWAFLDGDHAMA
CCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHH
QARALDARRQSGAALGPLHGLPVGVKDVIDVRGMPTGLGLAGGAGRVAQADAFVAARLRA
HHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
AGAILMGKTATAEAAFLEPPATRNPHDPARTPGGSSSGSAAAVAAGMVPLAIGTQTGGSV
HCCEEECCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCHHEEECCCCCCCH
IRPAAFCGIAGFKPSFGLIPRSGVLTQSPSLDTMGVFGRSVADVALLAEALAGHDPADPA
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHH
SLLQPAPRLPETAASDPPVTPTLAFVRPPGWEAAHEEMRLALQELAELLGRDCLALELPA
HHHCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEHHH
VFGAAADLRRRINLAEMAHHYAGLAGRAALSPLLSEALAEGRRIPAADYLAALEARTALR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
AGLEPIFHRCDAILCPAAPGPAPDRATTGDAIFNGLWTFCGLPAVTLPLFVSSDGLPMGA
HHHHHHHHHCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHEECCCCCCCCC
QLVGPPGGDARLLRTARWLVQRLAQTAPETAP
EEECCCCCHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
GRADRNGGLLALTGAELAERLASGAVRAVELVEACLAQIAAREPEVQAWAFLDGDHAMA
CCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCHHHHH
QARALDARRQSGAALGPLHGLPVGVKDVIDVRGMPTGLGLAGGAGRVAQADAFVAARLRA
HHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
AGAILMGKTATAEAAFLEPPATRNPHDPARTPGGSSSGSAAAVAAGMVPLAIGTQTGGSV
HCCEEECCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCHHEEECCCCCCCH
IRPAAFCGIAGFKPSFGLIPRSGVLTQSPSLDTMGVFGRSVADVALLAEALAGHDPADPA
HHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHH
SLLQPAPRLPETAASDPPVTPTLAFVRPPGWEAAHEEMRLALQELAELLGRDCLALELPA
HHHCCCCCCCCCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEHHH
VFGAAADLRRRINLAEMAHHYAGLAGRAALSPLLSEALAEGRRIPAADYLAALEARTALR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
AGLEPIFHRCDAILCPAAPGPAPDRATTGDAIFNGLWTFCGLPAVTLPLFVSSDGLPMGA
HHHHHHHHHCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHEECCCCCCCCC
QLVGPPGGDARLLRTARWLVQRLAQTAPETAP
EEECCCCCHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9809753 [H]