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The map label for this gene is alkJ [H]

Identifier: 77465297

GI number: 77465297

Start: 350977

End: 352578

Strand: Reverse

Name: alkJ [H]

Synonym: RSP_3292

Alternate gene names: 77465297

Gene position: 352578-350977 (Counterclockwise)

Preceding gene: 77465298

Following gene: 77465296

Centisome position: 37.39

GC content: 68.73

Gene sequence:

>1602_bases
ATGGAGGCCGATTACATCATCGTGGGGGCGGGCAGCGCGGGCTGCGTACTGGCGAACCGCCTGTCCAAGGATCCCTCGAA
CCGCGTGCTGCTGATCGAGGCGGGCAAGCGCGACAATTACCACTGGGTGCATATCCCGGTGGGCTATCTCTACTGCATCA
ACAATCCCCGCACCGACTGGTGCTTCACCACCGAGCCCGAGGAAGGGCTGGAAGGTCGCAGCCTGATCTACCCCCGCGGC
AAGGTGCTCGGCGGCTGTTCCTCGATCAACGGCATGATCTACATGCGCGGTCAGGCCGAGGATTACGACGGCTGGCGCCA
GATGGGCTGCACCGGCTGGGGCTGGGACGATGTGCTGCCCCTCTTCCGCCGCCAGCAGGACCATCACCGCGGCGAAAGCG
AACATCACGGCGCGGGCGGCGAATGGCGGGTGGAACGGGCGCGGGTCCGCTGGGCGGTACTCGATGCTTTCCTCGATGCG
GCCGAGCAGGCGGGCATCCCGCGGACCGAGGATTTTAACCGCGGCTCGAACGAGGGCGGCGGCTATTTCGACGTGAACCA
GAGGTCCGGCATCCGCTGGAACACGGCCAAGGCCTTCCTGAAGCCCGCCCTCTCCCGCCCGAACCTGCGCGTCGTGACCG
AGGCGCAGGTTGAGCGGCTGATCGTCGAGGCGGGCGAGGTGCGGGGCGTGCTCTACCGGCAGGGCGGCACCCTGCACGAG
GCCCGGGCGCGGCGCGAGACGGTGCTTGCGGCGGGTGCCATCGGCTCGCCGCACATTCTGGAGCTTTCGGGCATCGGCGA
TCCCGAGGTGCTGCGCGCGGCGGGCGTCGAGCCGCAGGTCGCCGTGCCGGGCGTAGGTGCGAACCTGCAGGATCACCTGC
AGCTCCGCCTCGTCTTCAAGGTGCGGGGCGTGCCCACGCTGAACGAGAAGGCCACCAGCCTCTTCGGTCGCGCCGCGATC
GGGGCGGAATATCTCCTGCGCCGCTCGGGGCCGATGTCGATGGCACCGAGTCAGGTCGGGATCTTCACCCGCTCCGGCTC
CGAGAAGGCCACGCCCGATCTCGAGTTCCATGTCCAGCCGGTCTCGCTCGACAAGTTCGGCGACAAGGTCCACCCCTTCC
CCGGCATGACGGCGAGCGTCTGCAACCTGCGCCCCGAAAGCCGCGGCAGCGTCCATCTGAAAAGCCCCGATCCCGCGCGC
CAGCCCGCCATCGCGCCGCATTATCTTTCGACCGAGGGCGACCGCGAGGTGGCGGTGCGCTCGATCCAGATTGCGCGCCA
TATCGCCTCGCAGCCCGCCTTCGCGCGGTTTCACCCCGAGGAATACCGTCCGGGAGCCGAGCACGACACGCGCGAGGCGC
TGGTCGCCGCCGCGGGCCGCATCGGCACCACGATCTTCCACCCGGTCGGCACCTGCCGCATGGGGTCGGATCCGGCGAGC
GTCGTCGATCCGCGGCTGAAGTTCCGGGCGCTCGGCGGCCTCAGGATCGCGGATGCGTCGATCATGCCGGCCATCACCTC
GGGAAACACCAACTCGCCCACCCTCATGATTGCCGAGAAGGCGGCCGAGATGATCCTCGAGGATGCCCGGCAGCGGGTTT
GA

Upstream 100 bases:

>100_bases
GTCTTCATGGCCTGCGTGCTGCTCTTCCGCCGCGGGATCGTGGGCGAGTTCTATGCCTCGCGCCTCGGCCGCCGGCTCGG
CTTCCGGTCGGAGAGCTGAG

Downstream 100 bases:

>100_bases
GGATGAAAGTTTCCCGGGAACCTGTGCGGGCGCCCGCGTGTTGAGGTTGTCAGTGTCCGGGTGAGCCCATTGCCGCCGAC
CGTTCAGTTCATCGATCTTC

Product: glucose-methanol-choline oxidoreductase

Products: Betaine Aldehyde; Reduced Acceptor. [C]

Alternate protein names: NA

Number of amino acids: Translated: 533; Mature: 533

Protein sequence:

>533_residues
MEADYIIVGAGSAGCVLANRLSKDPSNRVLLIEAGKRDNYHWVHIPVGYLYCINNPRTDWCFTTEPEEGLEGRSLIYPRG
KVLGGCSSINGMIYMRGQAEDYDGWRQMGCTGWGWDDVLPLFRRQQDHHRGESEHHGAGGEWRVERARVRWAVLDAFLDA
AEQAGIPRTEDFNRGSNEGGGYFDVNQRSGIRWNTAKAFLKPALSRPNLRVVTEAQVERLIVEAGEVRGVLYRQGGTLHE
ARARRETVLAAGAIGSPHILELSGIGDPEVLRAAGVEPQVAVPGVGANLQDHLQLRLVFKVRGVPTLNEKATSLFGRAAI
GAEYLLRRSGPMSMAPSQVGIFTRSGSEKATPDLEFHVQPVSLDKFGDKVHPFPGMTASVCNLRPESRGSVHLKSPDPAR
QPAIAPHYLSTEGDREVAVRSIQIARHIASQPAFARFHPEEYRPGAEHDTREALVAAAGRIGTTIFHPVGTCRMGSDPAS
VVDPRLKFRALGGLRIADASIMPAITSGNTNSPTLMIAEKAAEMILEDARQRV

Sequences:

>Translated_533_residues
MEADYIIVGAGSAGCVLANRLSKDPSNRVLLIEAGKRDNYHWVHIPVGYLYCINNPRTDWCFTTEPEEGLEGRSLIYPRG
KVLGGCSSINGMIYMRGQAEDYDGWRQMGCTGWGWDDVLPLFRRQQDHHRGESEHHGAGGEWRVERARVRWAVLDAFLDA
AEQAGIPRTEDFNRGSNEGGGYFDVNQRSGIRWNTAKAFLKPALSRPNLRVVTEAQVERLIVEAGEVRGVLYRQGGTLHE
ARARRETVLAAGAIGSPHILELSGIGDPEVLRAAGVEPQVAVPGVGANLQDHLQLRLVFKVRGVPTLNEKATSLFGRAAI
GAEYLLRRSGPMSMAPSQVGIFTRSGSEKATPDLEFHVQPVSLDKFGDKVHPFPGMTASVCNLRPESRGSVHLKSPDPAR
QPAIAPHYLSTEGDREVAVRSIQIARHIASQPAFARFHPEEYRPGAEHDTREALVAAAGRIGTTIFHPVGTCRMGSDPAS
VVDPRLKFRALGGLRIADASIMPAITSGNTNSPTLMIAEKAAEMILEDARQRV
>Mature_533_residues
MEADYIIVGAGSAGCVLANRLSKDPSNRVLLIEAGKRDNYHWVHIPVGYLYCINNPRTDWCFTTEPEEGLEGRSLIYPRG
KVLGGCSSINGMIYMRGQAEDYDGWRQMGCTGWGWDDVLPLFRRQQDHHRGESEHHGAGGEWRVERARVRWAVLDAFLDA
AEQAGIPRTEDFNRGSNEGGGYFDVNQRSGIRWNTAKAFLKPALSRPNLRVVTEAQVERLIVEAGEVRGVLYRQGGTLHE
ARARRETVLAAGAIGSPHILELSGIGDPEVLRAAGVEPQVAVPGVGANLQDHLQLRLVFKVRGVPTLNEKATSLFGRAAI
GAEYLLRRSGPMSMAPSQVGIFTRSGSEKATPDLEFHVQPVSLDKFGDKVHPFPGMTASVCNLRPESRGSVHLKSPDPAR
QPAIAPHYLSTEGDREVAVRSIQIARHIASQPAFARFHPEEYRPGAEHDTREALVAAAGRIGTTIFHPVGTCRMGSDPAS
VVDPRLKFRALGGLRIADASIMPAITSGNTNSPTLMIAEKAAEMILEDARQRV

Specific function: Converts aliphatic medium-chain-length alcohols into aldehydes. May be linked to the electron transfer chain [H]

COG id: COG2303

COG function: function code E; Choline dehydrogenase and related flavoproteins

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GMC oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI217272839, Length=533, Percent_Identity=40.9005628517824, Blast_Score=399, Evalue=1e-111,
Organism=Escherichia coli, GI1786503, Length=533, Percent_Identity=39.5872420262664, Blast_Score=348, Evalue=5e-97,
Organism=Caenorhabditis elegans, GI17532301, Length=542, Percent_Identity=32.6568265682657, Blast_Score=308, Evalue=4e-84,
Organism=Drosophila melanogaster, GI24642055, Length=580, Percent_Identity=36.0344827586207, Blast_Score=317, Evalue=1e-86,
Organism=Drosophila melanogaster, GI24642042, Length=571, Percent_Identity=35.0262697022767, Blast_Score=304, Evalue=1e-82,
Organism=Drosophila melanogaster, GI24642048, Length=573, Percent_Identity=34.7294938917976, Blast_Score=299, Evalue=3e-81,
Organism=Drosophila melanogaster, GI45551458, Length=570, Percent_Identity=33.5087719298246, Blast_Score=288, Evalue=5e-78,
Organism=Drosophila melanogaster, GI45549471, Length=570, Percent_Identity=33.5087719298246, Blast_Score=288, Evalue=5e-78,
Organism=Drosophila melanogaster, GI18859995, Length=560, Percent_Identity=33.3928571428571, Blast_Score=267, Evalue=1e-71,
Organism=Drosophila melanogaster, GI24642059, Length=567, Percent_Identity=33.3333333333333, Blast_Score=267, Evalue=1e-71,
Organism=Drosophila melanogaster, GI24642039, Length=569, Percent_Identity=33.0404217926186, Blast_Score=266, Evalue=2e-71,
Organism=Drosophila melanogaster, GI24642051, Length=566, Percent_Identity=32.3321554770318, Blast_Score=264, Evalue=1e-70,
Organism=Drosophila melanogaster, GI24650267, Length=575, Percent_Identity=33.7391304347826, Blast_Score=264, Evalue=1e-70,
Organism=Drosophila melanogaster, GI17137792, Length=565, Percent_Identity=32.9203539823009, Blast_Score=263, Evalue=3e-70,
Organism=Drosophila melanogaster, GI24642035, Length=573, Percent_Identity=31.7626527050611, Blast_Score=251, Evalue=1e-66,
Organism=Drosophila melanogaster, GI24642037, Length=572, Percent_Identity=33.5664335664336, Blast_Score=245, Evalue=6e-65,
Organism=Drosophila melanogaster, GI18859993, Length=573, Percent_Identity=30.1919720767888, Blast_Score=241, Evalue=9e-64,
Organism=Drosophila melanogaster, GI24645930, Length=575, Percent_Identity=29.3913043478261, Blast_Score=172, Evalue=4e-43,
Organism=Drosophila melanogaster, GI24642057, Length=579, Percent_Identity=27.979274611399, Blast_Score=159, Evalue=4e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012132
- InterPro:   IPR000172
- InterPro:   IPR007867 [H]

Pfam domain/function: PF05199 GMC_oxred_C; PF00732 GMC_oxred_N [H]

EC number: 1.1.99.1 [C]

Molecular weight: Translated: 58260; Mature: 58260

Theoretical pI: Translated: 7.90; Mature: 7.90

Prosite motif: PS00624 GMC_OXRED_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEADYIIVGAGSAGCVLANRLSKDPSNRVLLIEAGKRDNYHWVHIPVGYLYCINNPRTDW
CCCCEEEEECCCCCHHHHHHHCCCCCCCEEEEECCCCCCEEEEEEECEEEEEECCCCCCE
CFTTEPEEGLEGRSLIYPRGKVLGGCSSINGMIYMRGQAEDYDGWRQMGCTGWGWDDVLP
EEECCCCCCCCCCEEECCCCCEEECCCCCCCEEEEECCCCCCCHHHHCCCCCCCHHHHHH
LFRRQQDHHRGESEHHGAGGEWRVERARVRWAVLDAFLDAAEQAGIPRTEDFNRGSNEGG
HHHHHHHHHCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
GYFDVNQRSGIRWNTAKAFLKPALSRPNLRVVTEAQVERLIVEAGEVRGVLYRQGGTLHE
CEEECCCCCCCEEHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHCCCCEEEEECCCCHHH
ARARRETVLAAGAIGSPHILELSGIGDPEVLRAAGVEPQVAVPGVGANLQDHLQLRLVFK
HHHHHHHEEEECCCCCCCEEEEECCCCHHHHHHCCCCCCEECCCCCCCHHHCEEEEEEEE
VRGVPTLNEKATSLFGRAAIGAEYLLRRSGPMSMAPSQVGIFTRSGSEKATPDLEFHVQP
ECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEEE
VSLDKFGDKVHPFPGMTASVCNLRPESRGSVHLKSPDPARQPAIAPHYLSTEGDREVAVR
EEHHHCCCCCCCCCCCCHHHHCCCCCCCCEEEECCCCCCCCCCCCCCEECCCCCHHHHHH
SIQIARHIASQPAFARFHPEEYRPGAEHDTREALVAAAGRIGTTIFHPVGTCRMGSDPAS
HHHHHHHHHCCCCEECCCHHHCCCCCCCHHHHHHHHHHHHHCCEEECCCCCCCCCCCCHH
VVDPRLKFRALGGLRIADASIMPAITSGNTNSPTLMIAEKAAEMILEDARQRV
HCCCCCEEEECCCEEEECCCCCEEEECCCCCCCEEEEHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEADYIIVGAGSAGCVLANRLSKDPSNRVLLIEAGKRDNYHWVHIPVGYLYCINNPRTDW
CCCCEEEEECCCCCHHHHHHHCCCCCCCEEEEECCCCCCEEEEEEECEEEEEECCCCCCE
CFTTEPEEGLEGRSLIYPRGKVLGGCSSINGMIYMRGQAEDYDGWRQMGCTGWGWDDVLP
EEECCCCCCCCCCEEECCCCCEEECCCCCCCEEEEECCCCCCCHHHHCCCCCCCHHHHHH
LFRRQQDHHRGESEHHGAGGEWRVERARVRWAVLDAFLDAAEQAGIPRTEDFNRGSNEGG
HHHHHHHHHCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
GYFDVNQRSGIRWNTAKAFLKPALSRPNLRVVTEAQVERLIVEAGEVRGVLYRQGGTLHE
CEEECCCCCCCEEHHHHHHHHHHCCCCCEEEEEHHHHHHHHHHHCCCCEEEEECCCCHHH
ARARRETVLAAGAIGSPHILELSGIGDPEVLRAAGVEPQVAVPGVGANLQDHLQLRLVFK
HHHHHHHEEEECCCCCCCEEEEECCCCHHHHHHCCCCCCEECCCCCCCHHHCEEEEEEEE
VRGVPTLNEKATSLFGRAAIGAEYLLRRSGPMSMAPSQVGIFTRSGSEKATPDLEFHVQP
ECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCEEEEEE
VSLDKFGDKVHPFPGMTASVCNLRPESRGSVHLKSPDPARQPAIAPHYLSTEGDREVAVR
EEHHHCCCCCCCCCCCCHHHHCCCCCCCCEEEECCCCCCCCCCCCCCEECCCCCHHHHHH
SIQIARHIASQPAFARFHPEEYRPGAEHDTREALVAAAGRIGTTIFHPVGTCRMGSDPAS
HHHHHHHHHCCCCEECCCHHHCCCCCCCHHHHHHHHHHHHHCCEEECCCCCCCCCCCCHH
VVDPRLKFRALGGLRIADASIMPAITSGNTNSPTLMIAEKAAEMILEDARQRV
HCCCCCEEEECCCEEEECCCCCEEEECCCCCCCEEEEHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Coenzyme Q [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 7 {choline}} 1.1 {phenazine}} [C]

Substrates: Choline; Acceptor [C]

Specific reaction: Choline + Acceptor = Betaine Aldehyde + Reduced Acceptor. [C]

General reaction: Redox reaction [C]

Inhibitor: 2-Dimethylaminoethanol; Betaine aldehyde; Monoethanolamine; Semicarbazide [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1453953 [H]