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The map label for this gene is cobB [H]

Identifier: 77465198

GI number: 77465198

Start: 237174

End: 238499

Strand: Reverse

Name: cobB [H]

Synonym: RSP_3185

Alternate gene names: 77465198

Gene position: 238499-237174 (Counterclockwise)

Preceding gene: 77465199

Following gene: 77465197

Centisome position: 25.29

GC content: 74.06

Gene sequence:

>1326_bases
ATGTCGACCGCGTCCCTGTTGCCGCCCGGCCTCCTGATCGCAGCGCCCGGGTCGGGATCGGGGAAGACCACGGTGACGCT
GGGCCTGCTGCGGGCCTATCGCACGCAGGGGCTCGCCGTGCAGCCCTTCAAGAACGGGCCGGATTACATCGATCCGGCCT
TTCATCGGGCGGCGGCCGGGCGGGCTTCGGTCAATCTCGACGGCTGGGCGATGCCGGACGCGCTGCTCGCGGCGCTGGTC
CGGGCGGCGGAAGGGGCGGATCTGGCCCTGGCCGAAGGATCGATGGGTCTCTACGACGGCACCTCGCTGCCCGGGATGAG
CGGGCGCGGCTCGGCCGCCGAGATCGCACGGCGCTTCGGCTGGCCCGTGCTGATCGTCCTCGATGTGAGCGGGCAGGCGC
AGTCGGCGGCGGCGACCGCGCTCGGCTTCGCGCAGCATCCCGACGCACCGGCGCTCGGCGGCGTGATCCTCAACAACGTG
GCCAGCCCGCGCCACGAGACCCTGATCCGGCGCGAGATGGAGCGGCTGGGGCTGCCGGTGCGGGGCGCCCTGCCGCGGCG
GCGCGATCTGGCGCTGCCCGAGCGGCATCTGGGTCTGGTGCAGGCGGGCGAGCATCCCGATCTGGAGGGGGCCATCGGGC
AGTTCGCGGCTTTGCTGGCGGAGCATGCCGATCTCGACGCGATCCGGGCCGCCGCGGGCGACACCCGGGCCGGCCCGCCG
GGCGCCCTGCCCCGACCGCCGGCGCAGCGGATCGCGCTCGCGTCGGATGCGGCCTTCTCCTTCGTCTATCCGCATCTGAT
CGCGGGCTGGCGGGCGGCGGGGGCCGAGATCCTGCCCTTCTCGCCGCTCGCCGACGAGGCGCCGCCCGAGGCCGATCTCG
TCTGGCTGCCGGGCGGCTATCCCGAGCTGCACGCGGGCCGGCTGGCCGCGGCGGCAACTTTCCGGGCAGGCCTCTGCCGC
CATGCCGCGACGCGGCCCGTCCATGGCGAATGCGGCGGCTACATGGTGCTGGGCGAGGCGCTGATCGATGCGGCGGGAGA
ACGCCATGCGATGACCGGGCTTCTGGGCCTCGTCACGAGCTTCGAGCGGCGGCGGCTGCATCTGGGCTACCGCTCGGCGC
GGCTTCTGGCGCAGGCGCCCGGCCATGCGGCGGGCGCGCGCCTGCGGGGCCACGAGTTCCATTATTCCACCATCCTCGAA
CAGCCGGATGCGCCGCTGGCGGAGGTGCGGGATGCGGAGGGGCGGCCCGTGGCCGAGACCGGATCGAGCCGGGGGCCGGT
GACGGGGAGTTTCTTCCACCTGATCTGCGCCGAAGCGGAGGCGTAA

Upstream 100 bases:

>100_bases
GCGGGCTTGGACGGGTGACGGCCCGGAGGCGGTGCGCCTGCCGGAGCGCTGCGCCCCGGCCCCGGGCGGGCCTCGATCTG
CCGCCACAGAGGAAGAGCCC

Downstream 100 bases:

>100_bases
GCGAGATCCGCCTCCGCGGGGGCGGGGCGCCACTCCGGCACGGCACCGGCGGTCGCAGGAACCGTTACGGAACGTCACGG
CATGGTGCGGGGCCTTTCCC

Product: cobyrinic acid a,c-diamide synthase

Products: ADP; phosphate; Hydrogenobyrinic acid a,c diamide; L-glutamate

Alternate protein names: NA

Number of amino acids: Translated: 441; Mature: 440

Protein sequence:

>441_residues
MSTASLLPPGLLIAAPGSGSGKTTVTLGLLRAYRTQGLAVQPFKNGPDYIDPAFHRAAAGRASVNLDGWAMPDALLAALV
RAAEGADLALAEGSMGLYDGTSLPGMSGRGSAAEIARRFGWPVLIVLDVSGQAQSAAATALGFAQHPDAPALGGVILNNV
ASPRHETLIRREMERLGLPVRGALPRRRDLALPERHLGLVQAGEHPDLEGAIGQFAALLAEHADLDAIRAAAGDTRAGPP
GALPRPPAQRIALASDAAFSFVYPHLIAGWRAAGAEILPFSPLADEAPPEADLVWLPGGYPELHAGRLAAAATFRAGLCR
HAATRPVHGECGGYMVLGEALIDAAGERHAMTGLLGLVTSFERRRLHLGYRSARLLAQAPGHAAGARLRGHEFHYSTILE
QPDAPLAEVRDAEGRPVAETGSSRGPVTGSFFHLICAEAEA

Sequences:

>Translated_441_residues
MSTASLLPPGLLIAAPGSGSGKTTVTLGLLRAYRTQGLAVQPFKNGPDYIDPAFHRAAAGRASVNLDGWAMPDALLAALV
RAAEGADLALAEGSMGLYDGTSLPGMSGRGSAAEIARRFGWPVLIVLDVSGQAQSAAATALGFAQHPDAPALGGVILNNV
ASPRHETLIRREMERLGLPVRGALPRRRDLALPERHLGLVQAGEHPDLEGAIGQFAALLAEHADLDAIRAAAGDTRAGPP
GALPRPPAQRIALASDAAFSFVYPHLIAGWRAAGAEILPFSPLADEAPPEADLVWLPGGYPELHAGRLAAAATFRAGLCR
HAATRPVHGECGGYMVLGEALIDAAGERHAMTGLLGLVTSFERRRLHLGYRSARLLAQAPGHAAGARLRGHEFHYSTILE
QPDAPLAEVRDAEGRPVAETGSSRGPVTGSFFHLICAEAEA
>Mature_440_residues
STASLLPPGLLIAAPGSGSGKTTVTLGLLRAYRTQGLAVQPFKNGPDYIDPAFHRAAAGRASVNLDGWAMPDALLAALVR
AAEGADLALAEGSMGLYDGTSLPGMSGRGSAAEIARRFGWPVLIVLDVSGQAQSAAATALGFAQHPDAPALGGVILNNVA
SPRHETLIRREMERLGLPVRGALPRRRDLALPERHLGLVQAGEHPDLEGAIGQFAALLAEHADLDAIRAAAGDTRAGPPG
ALPRPPAQRIALASDAAFSFVYPHLIAGWRAAGAEILPFSPLADEAPPEADLVWLPGGYPELHAGRLAAAATFRAGLCRH
AATRPVHGECGGYMVLGEALIDAAGERHAMTGLLGLVTSFERRRLHLGYRSARLLAQAPGHAAGARLRGHEFHYSTILEQ
PDAPLAEVRDAEGRPVAETGSSRGPVTGSFFHLICAEAEA

Specific function: Responsible for the amidation of carboxylic groups at position A and C of either cobyrinic acid or hydrogenobrynic acid. NH(2) groups are provided by glutamine, and one molecule of ATP is hydrogenolyzed for each amidation [H]

COG id: COG1797

COG function: function code H; Cobyrinic acid a,c-diamide synthase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GATase cobBQ-type domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002586
- InterPro:   IPR004484
- InterPro:   IPR017929
- InterPro:   IPR011698 [H]

Pfam domain/function: PF01656 CbiA; PF07685 GATase_3 [H]

EC number: 6.3.5.9

Molecular weight: Translated: 45883; Mature: 45752

Theoretical pI: Translated: 6.84; Mature: 6.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTASLLPPGLLIAAPGSGSGKTTVTLGLLRAYRTQGLAVQPFKNGPDYIDPAFHRAAAG
CCCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCEEECCCCCCCCCCHHHHHHHCC
RASVNLDGWAMPDALLAALVRAAEGADLALAEGSMGLYDGTSLPGMSGRGSAAEIARRFG
CCEECCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHCC
WPVLIVLDVSGQAQSAAATALGFAQHPDAPALGGVILNNVASPRHETLIRREMERLGLPV
CCEEEEEECCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCC
RGALPRRRDLALPERHLGLVQAGEHPDLEGAIGQFAALLAEHADLDAIRAAAGDTRAGPP
CCCCCCCCCCCCCHHHCCCEECCCCCCHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCC
GALPRPPAQRIALASDAAFSFVYPHLIAGWRAAGAEILPFSPLADEAPPEADLVWLPGGY
CCCCCCHHHHEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEECCCCC
PELHAGRLAAAATFRAGLCRHAATRPVHGECGGYMVLGEALIDAAGERHAMTGLLGLVTS
CCHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHHCCCCHHHHHHHHHHHHH
FERRRLHLGYRSARLLAQAPGHAAGARLRGHEFHYSTILEQPDAPLAEVRDAEGRPVAET
HHHHHHHCCHHHHHHHHCCCCCHHCCCCCCCEEHHHHHHCCCCCCHHHHCCCCCCCHHHC
GSSRGPVTGSFFHLICAEAEA
CCCCCCCCHHHHEEEEECCCC
>Mature Secondary Structure 
STASLLPPGLLIAAPGSGSGKTTVTLGLLRAYRTQGLAVQPFKNGPDYIDPAFHRAAAG
CCCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCEEECCCCCCCCCCHHHHHHHCC
RASVNLDGWAMPDALLAALVRAAEGADLALAEGSMGLYDGTSLPGMSGRGSAAEIARRFG
CCEECCCCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHCC
WPVLIVLDVSGQAQSAAATALGFAQHPDAPALGGVILNNVASPRHETLIRREMERLGLPV
CCEEEEEECCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCC
RGALPRRRDLALPERHLGLVQAGEHPDLEGAIGQFAALLAEHADLDAIRAAAGDTRAGPP
CCCCCCCCCCCCCHHHCCCEECCCCCCHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCCC
GALPRPPAQRIALASDAAFSFVYPHLIAGWRAAGAEILPFSPLADEAPPEADLVWLPGGY
CCCCCCHHHHEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEECCCCC
PELHAGRLAAAATFRAGLCRHAATRPVHGECGGYMVLGEALIDAAGERHAMTGLLGLVTS
CCHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEHHHHHHCCCCHHHHHHHHHHHHH
FERRRLHLGYRSARLLAQAPGHAAGARLRGHEFHYSTILEQPDAPLAEVRDAEGRPVAET
HHHHHHHCCHHHHHHHHCCCCCHHCCCCCCCEEHHHHHHCCCCCCHHHHCCCCCCCHHHC
GSSRGPVTGSFFHLICAEAEA
CCCCCCCCHHHHEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; hydrogenobyrinic acid; L-glutamine; H2O

Specific reaction: 2 ATP + hydrogenobyrinic acid + 2 L-glutamine + 2 H2O = 2 ADP + 2 phosphate + hydrogenobyrinic acid a,c-diamide + 2 L-glutamate

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9256491 [H]