Click here to switch to the map view.

The map label for this gene is thlA [H]

Identifier: 77465197

GI number: 77465197

Start: 235853

End: 237013

Strand: Reverse

Name: thlA [H]

Synonym: RSP_3184

Alternate gene names: 77465197

Gene position: 237013-235853 (Counterclockwise)

Preceding gene: 77465198

Following gene: 77465196

Centisome position: 25.13

GC content: 73.13

Gene sequence:

>1161_bases
ATGGCAGCGGAACCGATCGTGATCGCGGGGGCGGCGCGGACGCCGATGGGCGCCTTTCAGGGTGCGCTCAAGGGCCGGAC
GGGGGTCGAGCTCGGCGCGGCGGCGATCTCGGCCGCGCTCGCGCGGGCGGGCCTCGCCCCCGAGGCGGTCGAGGAGGTCG
TGATGGGCTGCGTGCTGCCCGCGGGCCTCGGGCAGGCGCCGGCGCGGCAGGCGGCTCTGGGCGCGGGGCTGCCGCTCTCG
GTGCCCTGCGCTACGCTGAACAAGGTCTGCGGCTCGGGGATGAAGGCCGCCATGGCCGCGCATGACATGATCCGGGCGGG
CAGCGCGGGCATCGTCGTCGCGGGCGGTATGGAGAGCATGTCGAACGCGCCCTATCTGCTCGACAAGGCGCGCGGCGGCT
ACCGGATCGGGCATGGGCGCGTGCTGGACCACATGTTCCTCGACGGGCTCGAGGATGCCTACGACCGCGGCCGCGCCATG
GGCACCTTCGCCGAGGATTGCGCCGAGGCCTACCAGTTCACCCGCGACGCGCAGGATGCCTATGCGCTGGCCTCGCTGGC
CCGGGCGCAGGCGGCCATTGCCGAGGGGCGCTTCGCGGCCGAGGTGGTGGCAGTGGACGGGGTGGCGGTTGACGAGGCCC
CGGGCCGCGCCCGCCCCGAGAAGATCCCCCAGCTCAGGCCCGCCTTCCGCGAGGGCGGCACGGTGACGGCGGCCAACAGC
TCCTCGATCTCGGACGGGGCGGCGGCGCTCGTGATCGCCTCGGCCGGCGCGGCCGAGGCGCAGGGGCTGACCCCGCTCGC
CACGATCCGCGGCCATGCAAGCCATGCGCAGGCGCCGAACCTTTTTACCACGGCGCCGATCTTCGCCATCGGGAAGCTTC
TCGAGCGGTTGGGCTGGAGCGCGGCCGAGGTCGATCTCTTCGAGATCAACGAGGCTTTCGCAGTGGTGGCCATGGCCGCG
ATGCGCGACCTAGACCTGCCCCACGACAAGGTCAATGTGAACGGCGGCGCCTGCGCGCTCGGCCATCCGATCGGCTGCTC
GGGGGCGCGGATCGTGGTGACTTTGATCGAGGCGCTGCGGGCGCGGGGATTGAGACGCGGCGTGGCCTCGCTCTGCATCG
GCGGCGGCGAGGCGACGGCGCTGGCGGTGGAGGTGATCTGA

Upstream 100 bases:

>100_bases
ACCGTTACGGAACGTCACGGCATGGTGCGGGGCCTTTCCCCTTCCCCGGCGCGGGCAGGCCGGATTAGCCTCCGCCGGTC
AGCAGGCAGGGGAGGGCTTC

Downstream 100 bases:

>100_bases
TGGACCTTCGGGATCAGGCGGCGCTCGTCACCGGCGCGGGATCGGGCTTGGGCGAGGCCACGGCGCGGGCGCTGGCGGCG
GCGGGGGCGCGGGTGGCGCT

Product: putative thiolase

Products: NA

Alternate protein names: Acetoacetyl-CoA thiolase [H]

Number of amino acids: Translated: 386; Mature: 385

Protein sequence:

>386_residues
MAAEPIVIAGAARTPMGAFQGALKGRTGVELGAAAISAALARAGLAPEAVEEVVMGCVLPAGLGQAPARQAALGAGLPLS
VPCATLNKVCGSGMKAAMAAHDMIRAGSAGIVVAGGMESMSNAPYLLDKARGGYRIGHGRVLDHMFLDGLEDAYDRGRAM
GTFAEDCAEAYQFTRDAQDAYALASLARAQAAIAEGRFAAEVVAVDGVAVDEAPGRARPEKIPQLRPAFREGGTVTAANS
SSISDGAAALVIASAGAAEAQGLTPLATIRGHASHAQAPNLFTTAPIFAIGKLLERLGWSAAEVDLFEINEAFAVVAMAA
MRDLDLPHDKVNVNGGACALGHPIGCSGARIVVTLIEALRARGLRRGVASLCIGGGEATALAVEVI

Sequences:

>Translated_386_residues
MAAEPIVIAGAARTPMGAFQGALKGRTGVELGAAAISAALARAGLAPEAVEEVVMGCVLPAGLGQAPARQAALGAGLPLS
VPCATLNKVCGSGMKAAMAAHDMIRAGSAGIVVAGGMESMSNAPYLLDKARGGYRIGHGRVLDHMFLDGLEDAYDRGRAM
GTFAEDCAEAYQFTRDAQDAYALASLARAQAAIAEGRFAAEVVAVDGVAVDEAPGRARPEKIPQLRPAFREGGTVTAANS
SSISDGAAALVIASAGAAEAQGLTPLATIRGHASHAQAPNLFTTAPIFAIGKLLERLGWSAAEVDLFEINEAFAVVAMAA
MRDLDLPHDKVNVNGGACALGHPIGCSGARIVVTLIEALRARGLRRGVASLCIGGGEATALAVEVI
>Mature_385_residues
AAEPIVIAGAARTPMGAFQGALKGRTGVELGAAAISAALARAGLAPEAVEEVVMGCVLPAGLGQAPARQAALGAGLPLSV
PCATLNKVCGSGMKAAMAAHDMIRAGSAGIVVAGGMESMSNAPYLLDKARGGYRIGHGRVLDHMFLDGLEDAYDRGRAMG
TFAEDCAEAYQFTRDAQDAYALASLARAQAAIAEGRFAAEVVAVDGVAVDEAPGRARPEKIPQLRPAFREGGTVTAANSS
SISDGAAALVIASAGAAEAQGLTPLATIRGHASHAQAPNLFTTAPIFAIGKLLERLGWSAAEVDLFEINEAFAVVAMAAM
RDLDLPHDKVNVNGGACALGHPIGCSGARIVVTLIEALRARGLRRGVASLCIGGGEATALAVEVI

Specific function: Unknown

COG id: COG0183

COG function: function code I; Acetyl-CoA acetyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiolase family [H]

Homologues:

Organism=Homo sapiens, GI4557237, Length=389, Percent_Identity=45.7583547557841, Blast_Score=312, Evalue=3e-85,
Organism=Homo sapiens, GI148539872, Length=393, Percent_Identity=45.0381679389313, Blast_Score=300, Evalue=1e-81,
Organism=Homo sapiens, GI167614485, Length=392, Percent_Identity=38.265306122449, Blast_Score=242, Evalue=4e-64,
Organism=Homo sapiens, GI4501853, Length=398, Percent_Identity=37.9396984924623, Blast_Score=219, Evalue=4e-57,
Organism=Homo sapiens, GI4504327, Length=417, Percent_Identity=30.9352517985612, Blast_Score=162, Evalue=4e-40,
Organism=Homo sapiens, GI194353979, Length=386, Percent_Identity=31.0880829015544, Blast_Score=139, Evalue=4e-33,
Organism=Escherichia coli, GI87082165, Length=390, Percent_Identity=47.6923076923077, Blast_Score=329, Evalue=2e-91,
Organism=Escherichia coli, GI1788554, Length=388, Percent_Identity=50.5154639175258, Blast_Score=318, Evalue=3e-88,
Organism=Escherichia coli, GI1787663, Length=397, Percent_Identity=38.0352644836272, Blast_Score=218, Evalue=6e-58,
Organism=Escherichia coli, GI48994986, Length=406, Percent_Identity=37.4384236453202, Blast_Score=195, Evalue=4e-51,
Organism=Escherichia coli, GI1788683, Length=401, Percent_Identity=30.1745635910224, Blast_Score=142, Evalue=5e-35,
Organism=Caenorhabditis elegans, GI25147385, Length=385, Percent_Identity=41.8181818181818, Blast_Score=280, Evalue=1e-75,
Organism=Caenorhabditis elegans, GI17535921, Length=394, Percent_Identity=42.1319796954315, Blast_Score=277, Evalue=8e-75,
Organism=Caenorhabditis elegans, GI133906874, Length=395, Percent_Identity=37.2151898734177, Blast_Score=257, Evalue=6e-69,
Organism=Caenorhabditis elegans, GI17535917, Length=392, Percent_Identity=34.4387755102041, Blast_Score=192, Evalue=2e-49,
Organism=Caenorhabditis elegans, GI17551802, Length=420, Percent_Identity=30, Blast_Score=166, Evalue=2e-41,
Organism=Saccharomyces cerevisiae, GI6325229, Length=398, Percent_Identity=45.9798994974874, Blast_Score=324, Evalue=1e-89,
Organism=Saccharomyces cerevisiae, GI6322031, Length=394, Percent_Identity=33.756345177665, Blast_Score=178, Evalue=1e-45,
Organism=Drosophila melanogaster, GI24655093, Length=388, Percent_Identity=46.1340206185567, Blast_Score=323, Evalue=1e-88,
Organism=Drosophila melanogaster, GI24640423, Length=389, Percent_Identity=46.0154241645244, Blast_Score=303, Evalue=1e-82,
Organism=Drosophila melanogaster, GI17648125, Length=399, Percent_Identity=35.5889724310777, Blast_Score=216, Evalue=2e-56,
Organism=Drosophila melanogaster, GI17137578, Length=422, Percent_Identity=30.3317535545024, Blast_Score=152, Evalue=4e-37,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002155
- InterPro:   IPR016039
- InterPro:   IPR016038
- InterPro:   IPR020615
- InterPro:   IPR020610
- InterPro:   IPR020617
- InterPro:   IPR020613
- InterPro:   IPR020616 [H]

Pfam domain/function: PF02803 Thiolase_C; PF00108 Thiolase_N [H]

EC number: =2.3.1.9 [H]

Molecular weight: Translated: 38982; Mature: 38851

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: PS00099 THIOLASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAEPIVIAGAARTPMGAFQGALKGRTGVELGAAAISAALARAGLAPEAVEEVVMGCVLP
CCCCCEEEECCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCC
AGLGQAPARQAALGAGLPLSVPCATLNKVCGSGMKAAMAAHDMIRAGSAGIVVAGGMESM
CCCCCCCHHHHHHCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEECCHHHH
SNAPYLLDKARGGYRIGHGRVLDHMFLDGLEDAYDRGRAMGTFAEDCAEAYQFTRDAQDA
CCCCEEEECCCCCEEECCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
YALASLARAQAAIAEGRFAAEVVAVDGVAVDEAPGRARPEKIPQLRPAFREGGTVTAANS
HHHHHHHHHHHHHHCCCHHEEEEEECCEEECCCCCCCCCHHCCCCCHHHHCCCEEEECCC
SSISDGAAALVIASAGAAEAQGLTPLATIRGHASHAQAPNLFTTAPIFAIGKLLERLGWS
CCCCCCCEEEEEEECCCCHHCCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCC
AAEVDLFEINEAFAVVAMAAMRDLDLPHDKVNVNGGACALGHPIGCSGARIVVTLIEALR
HHEEEHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCEEECCCCCCCCCHHHHHHHHHHHH
ARGLRRGVASLCIGGGEATALAVEVI
HHHHHHHHHHHHCCCCCEEEEEEEEC
>Mature Secondary Structure 
AAEPIVIAGAARTPMGAFQGALKGRTGVELGAAAISAALARAGLAPEAVEEVVMGCVLP
CCCCEEEECCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCC
AGLGQAPARQAALGAGLPLSVPCATLNKVCGSGMKAAMAAHDMIRAGSAGIVVAGGMESM
CCCCCCCHHHHHHCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEECCHHHH
SNAPYLLDKARGGYRIGHGRVLDHMFLDGLEDAYDRGRAMGTFAEDCAEAYQFTRDAQDA
CCCCEEEECCCCCEEECCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
YALASLARAQAAIAEGRFAAEVVAVDGVAVDEAPGRARPEKIPQLRPAFREGGTVTAANS
HHHHHHHHHHHHHHCCCHHEEEEEECCEEECCCCCCCCCHHCCCCCHHHHCCCEEEECCC
SSISDGAAALVIASAGAAEAQGLTPLATIRGHASHAQAPNLFTTAPIFAIGKLLERLGWS
CCCCCCCEEEEEEECCCCHHCCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCC
AAEVDLFEINEAFAVVAMAAMRDLDLPHDKVNVNGGACALGHPIGCSGARIVVTLIEALR
HHEEEHHHHHHHHHHHHHHHHHHCCCCCCEEECCCCEEECCCCCCCCCHHHHHHHHHHHH
ARGLRRGVASLCIGGGEATALAVEVI
HHHHHHHHHHHHCCCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7867955; 11075929; 11466286; 1685080 [H]