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The map label for this gene is hmp [H]

Identifier: 77461909

GI number: 77461909

Start: 6375166

End: 6377196

Strand: Reverse

Name: hmp [H]

Synonym: Pfl01_5689

Alternate gene names: 77461909

Gene position: 6377196-6375166 (Counterclockwise)

Preceding gene: 77461910

Following gene: 77461900

Centisome position: 99.05

GC content: 62.19

Gene sequence:

>2031_bases
ATGGAACGTTCACCGTGGCACGCTGGCGAGCAACAGTTGCAGGCCCATGTCGGCGTTGCCGAGCGCATGGAGGAATTCGG
TCGTAAGGTGATTCGCACCTGGATGCCGGATCAACACCGCCAGTTCTATCAGCAATTGCCTTTCATGCTGTACGGCGCGG
TGGATGCCGAAGGTCGCCCCTGGGCCAGTGTGCTGGAGGGGGAGCCGGGCTTTGCTCACTCTCCCGAGCCGACGCAACTG
CAGTTCGCCAGTCTGCCGGCTGCCGACGATCCGGCACAGTTACAGGCTGGCGCAGCGATCGGTCTGCTTGGGATCGAACT
GCACACCCGCCGGCGCAATCGGCTCAATGGCCACATCGATAATCTCGACGCGAACGGCTTCGGGCTGACGGTCGATCAGT
CGTTCGGCAACTGCCCGCAGTACATTCAGTTGCGCCAGTTTCAGCGAGTGCCGCTGACGGATCCGCAGACCCGCAAAGCC
GAACACCGTGATAGCCTCGACGCCGCCGCCATTGCGCTGATTGAAAGCGCTGATACTTTCTTCGTCGCCAGTTACGTCGA
CGTCGACGGCGAGCGTTCGGTGGATGTTTCCCACCGCGGCGGTCAGCCCGGTTTTGTGCGAATCGAGGGTAATCGCCTGA
CGATCCCTGATTTTGCCGGCAACCTGCACTTCAACACGCTGGGCAATCTGCTGCTCAATCCCTTGGCCGGGTTGCTTTTC
ATCGACTTTTCCACAGGCGATGTGTTGCAGCTCAGCGGTCGCACCGAGATCATTCTTGACGCGCCGCAGATCGAGGCCTT
CCAGGGCGCCGAACGGTTGTGGACTTTCGAGGTGGAAAAACTGGTTCGGCGTCCGGCAGCGCTGGCTCTTCGCTGGCGCT
TCGACGGCATGTCACCCACCAGCCTGCTGACCGGTAACTGGGCCCAGGCCGATGCACGATTGCAGGCCAAGGCATTGGGG
GACAGCTGGCGGCCATTGCGCGTGGCAAAAGTCGAAATGGAAAGCCGTCACATCCGCTCGATCTATCTGGAGCCGAACGA
TGGCGCAGGACTGCCGGTGTTTCTCGCCGGGCAGCATCTGCCGCTGCGGTTCACCCTTGATGGCGAGGTGCACATCCGCA
CCTACAGCCTGTCCGGCGCGCCGTCGGACGATTTTTTCCGGATCAGTGTCAAGCGCGAAGGACGGGTGTCGACGCACCTG
CATGAGCAGATTCGCGTAGGCGATGTGCTGGAGGCGCGCTTGCCTCAAGGACATTTCACCGTCGCGGCACTGGAGCGCAA
GCCATTGGTGCTGCTCGCCGCCGGCGTCGGCATCACGCCGTTGCTGTCGATGCTGCGTGAGGTGGTGTATCAGGGCCTGC
GGACGCGACGGATTCGTCCGACCCTGTTTGTTCAGAGTTCCCGCAGCCTCGCCGACCAGCCGTTCCGCGCAGAGGTGGAT
CGGTTGCTGGAGGATGCCGGTGATGCCGTGAAAGTGTTGCGAGTGCTAAGTCAGCCGGAAGACGATCTGGTAGAAGGAAA
AGACTTCGACCTGCGTGGTCGTATCGATGGCGATCTGTTGCGCAATCTGCTGACCGACGAGGACTTCGATCAGGTCGACT
TCGTGCTCTGCGGTCCCGGCGGTTTTACCCAGGGTATTTACGACATCTTGCGCGAACTGAATGTGCGGGACGGGCAGATC
CATGCCGAAACCTTCGGCCCTTCGACCCTCAAGCGCCAAGCCGATCCGGACGCGATCGTGATGGAACAACCGCCGGCTGC
GACCACGTCGGTGCCGGTGCTGTTCGAGCGTTCGGCCAAGGAAGCGCGCTGGCAGCCCGACGGCGGCAGTTTGCTGGAGC
TGGCGGAAAGCCGCGGCCTGCGTCCGGAATTCAGTTGTCGCGGTGGTTCGTGCGGCACCTGCAAGACGCGGCTGATCAGC
GGCGCGGTGAATTATCCACAGCCTCCGGCTGAAGTACCGGAGGAAGGGCAGGTTTTGATCTGCTGCGCGGTGCCGGCCCA
GAGTGCCCAGCCGCTGGTGCTGGATCTGTAA

Upstream 100 bases:

>100_bases
AAGCGCTGCCCGGTTTTGTCGGCATGCCGCGCACCGTTGCCGGCCTGCAAACCACCGTCTGACATTCAATCTGCGCCGAC
GGCGCAGGGGAGAGACCGTG

Downstream 100 bases:

>100_bases
CGGATCAGGCGTAGGACAACGCCTTTTCCTCCAGCAGCTCCTGATACAGCTCGGTCCATTTGCGGCCCATTTCATCGGCT
GTGAATAACTGCCGATAGCG

Product: pyridoxamine 5'-phosphate oxidase-like protein

Products: NA

Alternate protein names: Flavohemoglobin; Hemoglobin-like protein; Nitric oxide dioxygenase; NO oxygenase; NOD [H]

Number of amino acids: Translated: 676; Mature: 676

Protein sequence:

>676_residues
MERSPWHAGEQQLQAHVGVAERMEEFGRKVIRTWMPDQHRQFYQQLPFMLYGAVDAEGRPWASVLEGEPGFAHSPEPTQL
QFASLPAADDPAQLQAGAAIGLLGIELHTRRRNRLNGHIDNLDANGFGLTVDQSFGNCPQYIQLRQFQRVPLTDPQTRKA
EHRDSLDAAAIALIESADTFFVASYVDVDGERSVDVSHRGGQPGFVRIEGNRLTIPDFAGNLHFNTLGNLLLNPLAGLLF
IDFSTGDVLQLSGRTEIILDAPQIEAFQGAERLWTFEVEKLVRRPAALALRWRFDGMSPTSLLTGNWAQADARLQAKALG
DSWRPLRVAKVEMESRHIRSIYLEPNDGAGLPVFLAGQHLPLRFTLDGEVHIRTYSLSGAPSDDFFRISVKREGRVSTHL
HEQIRVGDVLEARLPQGHFTVAALERKPLVLLAAGVGITPLLSMLREVVYQGLRTRRIRPTLFVQSSRSLADQPFRAEVD
RLLEDAGDAVKVLRVLSQPEDDLVEGKDFDLRGRIDGDLLRNLLTDEDFDQVDFVLCGPGGFTQGIYDILRELNVRDGQI
HAETFGPSTLKRQADPDAIVMEQPPAATTSVPVLFERSAKEARWQPDGGSLLELAESRGLRPEFSCRGGSCGTCKTRLIS
GAVNYPQPPAEVPEEGQVLICCAVPAQSAQPLVLDL

Sequences:

>Translated_676_residues
MERSPWHAGEQQLQAHVGVAERMEEFGRKVIRTWMPDQHRQFYQQLPFMLYGAVDAEGRPWASVLEGEPGFAHSPEPTQL
QFASLPAADDPAQLQAGAAIGLLGIELHTRRRNRLNGHIDNLDANGFGLTVDQSFGNCPQYIQLRQFQRVPLTDPQTRKA
EHRDSLDAAAIALIESADTFFVASYVDVDGERSVDVSHRGGQPGFVRIEGNRLTIPDFAGNLHFNTLGNLLLNPLAGLLF
IDFSTGDVLQLSGRTEIILDAPQIEAFQGAERLWTFEVEKLVRRPAALALRWRFDGMSPTSLLTGNWAQADARLQAKALG
DSWRPLRVAKVEMESRHIRSIYLEPNDGAGLPVFLAGQHLPLRFTLDGEVHIRTYSLSGAPSDDFFRISVKREGRVSTHL
HEQIRVGDVLEARLPQGHFTVAALERKPLVLLAAGVGITPLLSMLREVVYQGLRTRRIRPTLFVQSSRSLADQPFRAEVD
RLLEDAGDAVKVLRVLSQPEDDLVEGKDFDLRGRIDGDLLRNLLTDEDFDQVDFVLCGPGGFTQGIYDILRELNVRDGQI
HAETFGPSTLKRQADPDAIVMEQPPAATTSVPVLFERSAKEARWQPDGGSLLELAESRGLRPEFSCRGGSCGTCKTRLIS
GAVNYPQPPAEVPEEGQVLICCAVPAQSAQPLVLDL
>Mature_676_residues
MERSPWHAGEQQLQAHVGVAERMEEFGRKVIRTWMPDQHRQFYQQLPFMLYGAVDAEGRPWASVLEGEPGFAHSPEPTQL
QFASLPAADDPAQLQAGAAIGLLGIELHTRRRNRLNGHIDNLDANGFGLTVDQSFGNCPQYIQLRQFQRVPLTDPQTRKA
EHRDSLDAAAIALIESADTFFVASYVDVDGERSVDVSHRGGQPGFVRIEGNRLTIPDFAGNLHFNTLGNLLLNPLAGLLF
IDFSTGDVLQLSGRTEIILDAPQIEAFQGAERLWTFEVEKLVRRPAALALRWRFDGMSPTSLLTGNWAQADARLQAKALG
DSWRPLRVAKVEMESRHIRSIYLEPNDGAGLPVFLAGQHLPLRFTLDGEVHIRTYSLSGAPSDDFFRISVKREGRVSTHL
HEQIRVGDVLEARLPQGHFTVAALERKPLVLLAAGVGITPLLSMLREVVYQGLRTRRIRPTLFVQSSRSLADQPFRAEVD
RLLEDAGDAVKVLRVLSQPEDDLVEGKDFDLRGRIDGDLLRNLLTDEDFDQVDFVLCGPGGFTQGIYDILRELNVRDGQI
HAETFGPSTLKRQADPDAIVMEQPPAATTSVPVLFERSAKEARWQPDGGSLLELAESRGLRPEFSCRGGSCGTCKTRLIS
GAVNYPQPPAEVPEEGQVLICCAVPAQSAQPLVLDL

Specific function: Is involved in NO detoxification in an aerobic process, termed nitric oxide dioxygenase (NOD) reaction that utilizes O(2) and NAD(P)H to convert NO to nitrate, which protects the bacterium from various noxious nitrogen compounds. Therefore, plays a centra

COG id: COG3576

COG function: function code R; Predicted flavin-nucleotide-binding protein structurally related to pyridoxine 5'-phosphate oxidase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI1787658, Length=371, Percent_Identity=31.266846361186, Blast_Score=117, Evalue=3e-27,
Organism=Escherichia coli, GI1788903, Length=251, Percent_Identity=33.0677290836653, Blast_Score=109, Evalue=6e-25,
Organism=Escherichia coli, GI1788104, Length=359, Percent_Identity=24.7910863509749, Blast_Score=82, Evalue=8e-17,
Organism=Saccharomyces cerevisiae, GI6321673, Length=281, Percent_Identity=28.8256227758007, Blast_Score=104, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24655635, Length=226, Percent_Identity=27.8761061946903, Blast_Score=70, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017927
- InterPro:   IPR012292
- InterPro:   IPR009050
- InterPro:   IPR000971
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR001221
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00042 Globin; PF00175 NAD_binding_1 [H]

EC number: =1.14.12.17 [H]

Molecular weight: Translated: 74787; Mature: 74787

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: PS00197 2FE2S_FER_1 ; PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERSPWHAGEQQLQAHVGVAERMEEFGRKVIRTWMPDQHRQFYQQLPFMLYGAVDAEGRP
CCCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCEEEEEEECCCCCC
WASVLEGEPGFAHSPEPTQLQFASLPAADDPAQLQAGAAIGLLGIELHTRRRNRLNGHID
HHHHHCCCCCCCCCCCCCEEEEEECCCCCCHHHHHCCCEEEEEEEEEEHHHHHHCCCCCC
NLDANGFGLTVDQSFGNCPQYIQLRQFQRVPLTDPQTRKAEHRDSLDAAAIALIESADTF
CCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHCCHHHHHHHHHCCCCCE
FVASYVDVDGERSVDVSHRGGQPGFVRIEGNRLTIPDFAGNLHFNTLGNLLLNPLAGLLF
EEEEEEECCCCCCEEECCCCCCCCEEEEECCEEECCCCCCCEEHHHHHHHHHHHCCEEEE
IDFSTGDVLQLSGRTEIILDAPQIEAFQGAERLWTFEVEKLVRRPAALALRWRFDGMSPT
EEECCCCEEEECCCEEEEEECCCHHHHCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCC
SLLTGNWAQADARLQAKALGDSWRPLRVAKVEMESRHIRSIYLEPNDGAGLPVFLAGQHL
CEECCCCHHCCHHEEHHHCCCCCCCEEEEEEECCCCEEEEEEEECCCCCCCEEEEECCCC
PLRFTLDGEVHIRTYSLSGAPSDDFFRISVKREGRVSTHLHEQIRVGDVLEARLPQGHFT
EEEEEECCEEEEEEEEECCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCEE
VAALERKPLVLLAAGVGITPLLSMLREVVYQGLRTRRIRPTLFVQSSRSLADQPFRAEVD
EEEECCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHCCCCHHHHHH
RLLEDAGDAVKVLRVLSQPEDDLVEGKDFDLRGRIDGDLLRNLLTDEDFDQVDFVLCGPG
HHHHCCCHHHHHHHHHCCCCHHHCCCCCCEEECCCCHHHHHHHHCCCCCCCCCEEEECCC
GFTQGIYDILRELNVRDGQIHAETFGPSTLKRQADPDAIVMEQPPAATTSVPVLFERSAK
CCHHHHHHHHHHCCCCCCEEEEECCCCHHHHHCCCCCEEEEECCCCCCCCCCEEEECCCH
EARWQPDGGSLLELAESRGLRPEFSCRGGSCGTCKTRLISGAVNYPQPPAEVPEEGQVLI
HCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCEEE
CCAVPAQSAQPLVLDL
EEECCCCCCCCEEEEC
>Mature Secondary Structure
MERSPWHAGEQQLQAHVGVAERMEEFGRKVIRTWMPDQHRQFYQQLPFMLYGAVDAEGRP
CCCCCCCCCHHHHHHHHCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCEEEEEEECCCCCC
WASVLEGEPGFAHSPEPTQLQFASLPAADDPAQLQAGAAIGLLGIELHTRRRNRLNGHID
HHHHHCCCCCCCCCCCCCEEEEEECCCCCCHHHHHCCCEEEEEEEEEEHHHHHHCCCCCC
NLDANGFGLTVDQSFGNCPQYIQLRQFQRVPLTDPQTRKAEHRDSLDAAAIALIESADTF
CCCCCCCEEEEECCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHCCHHHHHHHHHCCCCCE
FVASYVDVDGERSVDVSHRGGQPGFVRIEGNRLTIPDFAGNLHFNTLGNLLLNPLAGLLF
EEEEEEECCCCCCEEECCCCCCCCEEEEECCEEECCCCCCCEEHHHHHHHHHHHCCEEEE
IDFSTGDVLQLSGRTEIILDAPQIEAFQGAERLWTFEVEKLVRRPAALALRWRFDGMSPT
EEECCCCEEEECCCEEEEEECCCHHHHCCHHHHHHHHHHHHHCCCCEEEEEEEECCCCCC
SLLTGNWAQADARLQAKALGDSWRPLRVAKVEMESRHIRSIYLEPNDGAGLPVFLAGQHL
CEECCCCHHCCHHEEHHHCCCCCCCEEEEEEECCCCEEEEEEEECCCCCCCEEEEECCCC
PLRFTLDGEVHIRTYSLSGAPSDDFFRISVKREGRVSTHLHEQIRVGDVLEARLPQGHFT
EEEEEECCEEEEEEEEECCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCEE
VAALERKPLVLLAAGVGITPLLSMLREVVYQGLRTRRIRPTLFVQSSRSLADQPFRAEVD
EEEECCCCEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHCCCCHHHHHH
RLLEDAGDAVKVLRVLSQPEDDLVEGKDFDLRGRIDGDLLRNLLTDEDFDQVDFVLCGPG
HHHHCCCHHHHHHHHHCCCCHHHCCCCCCEEECCCCHHHHHHHHCCCCCCCCCEEEECCC
GFTQGIYDILRELNVRDGQIHAETFGPSTLKRQADPDAIVMEQPPAATTSVPVLFERSAK
CCHHHHHHHHHHCCCCCCEEEEECCCCHHHHHCCCCCEEEEECCCCCCCCCCEEEECCCH
EARWQPDGGSLLELAESRGLRPEFSCRGGSCGTCKTRLISGAVNYPQPPAEVPEEGQVLI
HCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCEEE
CCAVPAQSAQPLVLDL
EEECCCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8682784; 9384377 [H]