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The map label for this gene is amtB [H]

Identifier: 77461726

GI number: 77461726

Start: 6170031

End: 6171368

Strand: Reverse

Name: amtB [H]

Synonym: Pfl01_5505

Alternate gene names: 77461726

Gene position: 6171368-6170031 (Counterclockwise)

Preceding gene: 77461727

Following gene: 77461725

Centisome position: 95.85

GC content: 62.71

Gene sequence:

>1338_bases
ATGACTCTGCGTAAATTCGCAGGGCTAGGAGCCCTGTTGTCTATCGTAATGCCCGGCCTGGCCATGGCGGCAGACGAAGT
GGCAGCTCCAGTCCTGAACTCCGGCGACACCGCCTGGATGCTCACCTCCACAGCCCTCGTGCTGTTCATGACCATTCCCG
GCCTCGCGCTGTTCTACGGCGGCATGGTCCGGTCGAAAAACATTCTTTCCGTGATGATGCAGTGCTTTGCCATTACCGGT
CTGATCAGCGTCCTGTGGGTCATTTATGGCTACAGCATCGCGTTCGACACCACCGGCATGGAGCAGGGCGTCGTCAACTT
CAATTCGTTCGTGGGCGGTTTCGGCAAGGCGTTCCTGGCCGGTGTCACGCCAGCGAGCCTGACCGGCCCGGCGGCGCTGT
TCCCCGAGGCGGTCTTCATCACCTTCCAGATGACCTTCGCGATCATCACCCCGGCGCTGATCGTCGGTGCATTCGCCGAG
CGGATGAAGTTCTCCGCGATGCTGATCTTCATGGGCATCTGGTTCACCCTGGTTTATGCACCGATCGCGCACATGGTCTG
GTCCGGCAACGGCGGCCTGATGTGGGACTGGGGCGTACTCGACTTCGCGGGCGGCACCGTGGTGCACATCAACGCCGGTG
TGGCCGGTCTGATTGCCTGCCTGGTACTGGGTAAACGCAAAGGCTTCCCGACCACGCCGATGGCGCCACACAATCTGGGT
TACACCCTGATGGGCGCGGCGATGCTGTGGGTCGGCTGGTTCGGCTTCAACGCCGGTTCCGCTGCGGCCGCCAACGGCAC
CGCCGGCATGGCGATGCTGGTGACCCAGATCGCGACCGCCGCTGCTGCACTGGGCTGGATGTTCGCCGAGTGGATCACCC
ACGGCAAACCTAGCGCACTGGGTATCGCATCCGGCGTCGTTGCAGGTCTGGTGGCGATCACTCCGGCCGCTGGCACCGTG
GGCCCGATGGGTGCCCTGGTCATCGGTCTGGCGGCAGGCGTGGTGTGCTTCTTCTGCGCCACCACCCTGAAACGCAAACT
CGGTTATGACGACTCCCTGGACGCCTTCGGCGTGCACGGTATCGGCGGTATCCTCGGCGCGATCCTGACCGGTGTGTTCG
CCGCACCGTCGCTGGGTGGCTTCGGCACCGTCACCGACATCGCCGCACAAGTCTGGATTCAGTGCAAAGGCGTCGGCTTC
ACCGTGATCTATACCGCGATCGTCACCTTCATCATCCTCAAGGTGCTGGATGCCGTGATGGGTCTGCGCGTGACCGAGGA
AGAAGAATCGGTCGGCCTGGATCTGGCACAACACAACGAGCGTGGCTACAACCTGTAA

Upstream 100 bases:

>100_bases
GTGGTCAATCTGGAACAGGCGATCCGCATCCGTACCGGCGAAACCGATACCGACGCGATCTAAGCGCCGCCACAAACCCA
ACGCCCCAGGAGAAAACAAT

Downstream 100 bases:

>100_bases
GCACGCGCCGCAAAAAACTTGCCCGGCTCGCCGGGCATTTTTTTGTCTGGAATTTGTCTTCCCCGATACAGCTGAAAGGA
TTTTTCGTACTGCTTTGGTC

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: Ammonia transporter [H]

Number of amino acids: Translated: 445; Mature: 444

Protein sequence:

>445_residues
MTLRKFAGLGALLSIVMPGLAMAADEVAAPVLNSGDTAWMLTSTALVLFMTIPGLALFYGGMVRSKNILSVMMQCFAITG
LISVLWVIYGYSIAFDTTGMEQGVVNFNSFVGGFGKAFLAGVTPASLTGPAALFPEAVFITFQMTFAIITPALIVGAFAE
RMKFSAMLIFMGIWFTLVYAPIAHMVWSGNGGLMWDWGVLDFAGGTVVHINAGVAGLIACLVLGKRKGFPTTPMAPHNLG
YTLMGAAMLWVGWFGFNAGSAAAANGTAGMAMLVTQIATAAAALGWMFAEWITHGKPSALGIASGVVAGLVAITPAAGTV
GPMGALVIGLAAGVVCFFCATTLKRKLGYDDSLDAFGVHGIGGILGAILTGVFAAPSLGGFGTVTDIAAQVWIQCKGVGF
TVIYTAIVTFIILKVLDAVMGLRVTEEEESVGLDLAQHNERGYNL

Sequences:

>Translated_445_residues
MTLRKFAGLGALLSIVMPGLAMAADEVAAPVLNSGDTAWMLTSTALVLFMTIPGLALFYGGMVRSKNILSVMMQCFAITG
LISVLWVIYGYSIAFDTTGMEQGVVNFNSFVGGFGKAFLAGVTPASLTGPAALFPEAVFITFQMTFAIITPALIVGAFAE
RMKFSAMLIFMGIWFTLVYAPIAHMVWSGNGGLMWDWGVLDFAGGTVVHINAGVAGLIACLVLGKRKGFPTTPMAPHNLG
YTLMGAAMLWVGWFGFNAGSAAAANGTAGMAMLVTQIATAAAALGWMFAEWITHGKPSALGIASGVVAGLVAITPAAGTV
GPMGALVIGLAAGVVCFFCATTLKRKLGYDDSLDAFGVHGIGGILGAILTGVFAAPSLGGFGTVTDIAAQVWIQCKGVGF
TVIYTAIVTFIILKVLDAVMGLRVTEEEESVGLDLAQHNERGYNL
>Mature_444_residues
TLRKFAGLGALLSIVMPGLAMAADEVAAPVLNSGDTAWMLTSTALVLFMTIPGLALFYGGMVRSKNILSVMMQCFAITGL
ISVLWVIYGYSIAFDTTGMEQGVVNFNSFVGGFGKAFLAGVTPASLTGPAALFPEAVFITFQMTFAIITPALIVGAFAER
MKFSAMLIFMGIWFTLVYAPIAHMVWSGNGGLMWDWGVLDFAGGTVVHINAGVAGLIACLVLGKRKGFPTTPMAPHNLGY
TLMGAAMLWVGWFGFNAGSAAAANGTAGMAMLVTQIATAAAALGWMFAEWITHGKPSALGIASGVVAGLVAITPAAGTVG
PMGALVIGLAAGVVCFFCATTLKRKLGYDDSLDAFGVHGIGGILGAILTGVFAAPSLGGFGTVTDIAAQVWIQCKGVGFT
VIYTAIVTFIILKVLDAVMGLRVTEEEESVGLDLAQHNERGYNL

Specific function: Involved in the uptake of ammonia [H]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=430, Percent_Identity=49.0697674418605, Blast_Score=385, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI17550180, Length=448, Percent_Identity=28.5714285714286, Blast_Score=128, Evalue=8e-30,
Organism=Caenorhabditis elegans, GI17550182, Length=455, Percent_Identity=29.4505494505495, Blast_Score=127, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI32564042, Length=447, Percent_Identity=28.6353467561521, Blast_Score=117, Evalue=9e-27,
Organism=Caenorhabditis elegans, GI133931100, Length=458, Percent_Identity=27.0742358078603, Blast_Score=115, Evalue=5e-26,
Organism=Saccharomyces cerevisiae, GI6325396, Length=423, Percent_Identity=35.9338061465721, Blast_Score=236, Evalue=6e-63,
Organism=Saccharomyces cerevisiae, GI6324187, Length=430, Percent_Identity=35.3488372093023, Blast_Score=223, Evalue=5e-59,
Organism=Saccharomyces cerevisiae, GI6321559, Length=417, Percent_Identity=33.5731414868106, Blast_Score=218, Evalue=2e-57,
Organism=Drosophila melanogaster, GI161078314, Length=439, Percent_Identity=26.879271070615, Blast_Score=113, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 46119; Mature: 45988

Theoretical pI: Translated: 6.50; Mature: 6.50

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
5.2 %Met     (Translated Protein)
6.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLRKFAGLGALLSIVMPGLAMAADEVAAPVLNSGDTAWMLTSTALVLFMTIPGLALFYG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHC
GMVRSKNILSVMMQCFAITGLISVLWVIYGYSIAFDTTGMEQGVVNFNSFVGGFGKAFLA
CHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEECCCCHHCCHHHHHHHHHHHHHHHH
GVTPASLTGPAALFPEAVFITFQMTFAIITPALIVGAFAERMKFSAMLIFMGIWFTLVYA
CCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PIAHMVWSGNGGLMWDWGVLDFAGGTVVHINAGVAGLIACLVLGKRKGFPTTPMAPHNLG
HHHHHHCCCCCCEEEECCHHCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCC
YTLMGAAMLWVGWFGFNAGSAAAANGTAGMAMLVTQIATAAAALGWMFAEWITHGKPSAL
HHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
GIASGVVAGLVAITPAAGTVGPMGALVIGLAAGVVCFFCATTLKRKLGYDDSLDAFGVHG
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCH
IGGILGAILTGVFAAPSLGGFGTVTDIAAQVWIQCKGVGFTVIYTAIVTFIILKVLDAVM
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHH
GLRVTEEEESVGLDLAQHNERGYNL
CCCCCCCHHHCCCCHHHCCCCCCCC
>Mature Secondary Structure 
TLRKFAGLGALLSIVMPGLAMAADEVAAPVLNSGDTAWMLTSTALVLFMTIPGLALFYG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHHHHHHHHHC
GMVRSKNILSVMMQCFAITGLISVLWVIYGYSIAFDTTGMEQGVVNFNSFVGGFGKAFLA
CHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHEEECCCCHHCCHHHHHHHHHHHHHHHH
GVTPASLTGPAALFPEAVFITFQMTFAIITPALIVGAFAERMKFSAMLIFMGIWFTLVYA
CCCCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PIAHMVWSGNGGLMWDWGVLDFAGGTVVHINAGVAGLIACLVLGKRKGFPTTPMAPHNLG
HHHHHHCCCCCCEEEECCHHCCCCCEEEEECCCHHHHHHHHHHHCCCCCCCCCCCCCCCC
YTLMGAAMLWVGWFGFNAGSAAAANGTAGMAMLVTQIATAAAALGWMFAEWITHGKPSAL
HHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
GIASGVVAGLVAITPAAGTVGPMGALVIGLAAGVVCFFCATTLKRKLGYDDSLDAFGVHG
HHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCH
IGGILGAILTGVFAAPSLGGFGTVTDIAAQVWIQCKGVGFTVIYTAIVTFIILKVLDAVM
HHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHEECCCCHHHHHHHHHHHHHHHHHHHHH
GLRVTEEEESVGLDLAQHNERGYNL
CCCCCCCHHHCCCCHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]