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The map label for this gene is rhtB [H]

Identifier: 77461697

GI number: 77461697

Start: 6144266

End: 6144898

Strand: Reverse

Name: rhtB [H]

Synonym: Pfl01_5476

Alternate gene names: 77461697

Gene position: 6144898-6144266 (Counterclockwise)

Preceding gene: 77461698

Following gene: 77461691

Centisome position: 95.44

GC content: 60.98

Gene sequence:

>633_bases
ATGGAGCTTCAAACATGGCTGGCGTTTTTTGCCGCCTGCTGGGTGATCAGTCTTTCTCCGGGTGCCGGCGCCATTGCGTC
GATGTCCAGCGGCCTGCAATACGGTTTCTGGCGTGGTTACTGGAACGCCCTGGGCCTGCAGATCGGCCTGGCCGTGCAGA
TTGCCATCGTCGGCGCCGGTGTCGGCGCGATCCTCACCGCTTCCGCCACAGCGTTTCACGCGATCAAATGGTTTGGTGTC
GCCTATCTGGTTTATCTCGCGATCAAGCAATGGCGCGCACAGCCGATGGACATGAGTGACGACGCGGCGGTCCGGCCGAT
CGGCAAACCGCTGGCTCTGGTGTTCCGTGGCTTTCTGGTGAATATCAGCAACCCCAAGGCGCTGGTGTTCATGCTGGCGG
TGCTGCCGCAGTTCATCAATCCGCATGCGCCGTTATTTATCCAGTACGTGGTGATCGGTGTGACCATGATCGCGGTCGAT
CTGATCGTCATGGCCGGCTACACCGGTCTGGCGTCGAAGGTGCTGCGTCTGCTGCGCACACCGACCCAGCAAAGACGTAT
GAACCGCACCTTTGCCGGGCTGTTCATCGGCGCGGCGGCGTTCATGGCGACGTTGCGCAAAGCGGCCGCATAA

Upstream 100 bases:

>100_bases
CGTTGGTCGGCGGCCACAACATCCGACAAAAAAACGGTAGTCATCCAGTCCAATCCGCATTAGTTTTGAGGATTGTCACG
AGTCCGAGTCGAGGTGTGCG

Downstream 100 bases:

>100_bases
AGCGTACAGGCACAAAAAAGGCGACCTTTTCAGGTCGCCTTTTTCGTTTCCGGCGGTTGATCAGCGCAGGATTACCGGCG
CGGTATCTCGCGGCAGATTG

Product: lysine exporter protein LysE/YggA

Products: homoserine lactone [Periplasm]; Proton [Cytoplasm]; homoserine [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 210; Mature: 210

Protein sequence:

>210_residues
MELQTWLAFFAACWVISLSPGAGAIASMSSGLQYGFWRGYWNALGLQIGLAVQIAIVGAGVGAILTASATAFHAIKWFGV
AYLVYLAIKQWRAQPMDMSDDAAVRPIGKPLALVFRGFLVNISNPKALVFMLAVLPQFINPHAPLFIQYVVIGVTMIAVD
LIVMAGYTGLASKVLRLLRTPTQQRRMNRTFAGLFIGAAAFMATLRKAAA

Sequences:

>Translated_210_residues
MELQTWLAFFAACWVISLSPGAGAIASMSSGLQYGFWRGYWNALGLQIGLAVQIAIVGAGVGAILTASATAFHAIKWFGV
AYLVYLAIKQWRAQPMDMSDDAAVRPIGKPLALVFRGFLVNISNPKALVFMLAVLPQFINPHAPLFIQYVVIGVTMIAVD
LIVMAGYTGLASKVLRLLRTPTQQRRMNRTFAGLFIGAAAFMATLRKAAA
>Mature_210_residues
MELQTWLAFFAACWVISLSPGAGAIASMSSGLQYGFWRGYWNALGLQIGLAVQIAIVGAGVGAILTASATAFHAIKWFGV
AYLVYLAIKQWRAQPMDMSDDAAVRPIGKPLALVFRGFLVNISNPKALVFMLAVLPQFINPHAPLFIQYVVIGVTMIAVD
LIVMAGYTGLASKVLRLLRTPTQQRRMNRTFAGLFIGAAAFMATLRKAAA

Specific function: Conducts the efflux of homoserine and homoserine lactone [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the rht family [H]

Homologues:

Organism=Escherichia coli, GI48994979, Length=209, Percent_Identity=40.6698564593301, Blast_Score=165, Evalue=2e-42,
Organism=Escherichia coli, GI1788099, Length=208, Percent_Identity=26.4423076923077, Blast_Score=67, Evalue=9e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004778
- InterPro:   IPR001123 [H]

Pfam domain/function: PF01810 LysE [H]

EC number: NA

Molecular weight: Translated: 22652; Mature: 22652

Theoretical pI: Translated: 10.94; Mature: 10.94

Prosite motif: PS00107 PROTEIN_KINASE_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELQTWLAFFAACWVISLSPGAGAIASMSSGLQYGFWRGYWNALGLQIGLAVQIAIVGAG
CCHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHEEEEHHHCC
VGAILTASATAFHAIKWFGVAYLVYLAIKQWRAQPMDMSDDAAVRPIGKPLALVFRGFLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
NISNPKALVFMLAVLPQFINPHAPLFIQYVVIGVTMIAVDLIVMAGYTGLASKVLRLLRT
CCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHC
PTQQRRMNRTFAGLFIGAAAFMATLRKAAA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MELQTWLAFFAACWVISLSPGAGAIASMSSGLQYGFWRGYWNALGLQIGLAVQIAIVGAG
CCHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHEEEEHHHCC
VGAILTASATAFHAIKWFGVAYLVYLAIKQWRAQPMDMSDDAAVRPIGKPLALVFRGFLV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
NISNPKALVFMLAVLPQFINPHAPLFIQYVVIGVTMIAVDLIVMAGYTGLASKVLRLLRT
CCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHC
PTQQRRMNRTFAGLFIGAAAFMATLRKAAA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; homoserine [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + homoserine lactone [Cytoplasm] = Proton [Cytoplasm] + homoserine lactone [Periplasm] Proton [Periplasm] + homoserine [Cytoplasm] = Proton [Cytoplasm] + homoserine [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]