Click here to switch to the map view.

The map label for this gene is ascD [H]

Identifier: 77461674

GI number: 77461674

Start: 6118523

End: 6119491

Strand: Reverse

Name: ascD [H]

Synonym: Pfl01_5453

Alternate gene names: 77461674

Gene position: 6119491-6118523 (Counterclockwise)

Preceding gene: 77461675

Following gene: 77461673

Centisome position: 95.05

GC content: 62.64

Gene sequence:

>969_bases
ATGCGTGTAACCCTGCAGCCCTCCGGAGCGGTGCTCGAGATACAGCCCGGAGAGCGGATTCTCGATGGCGCGCGGCGCCT
GGGCTACGAATGCCCGCGAAGCTGCCGCAATGGCAATTGCCATGTGTGTGCGGCACTGCTGGTGGAAGGCCGGGTCGAAC
AGGCCGGCAAGGTGCACGACCACGGCGAGTTCTACACTTGCATAGCGGAGCCGCTGGAAGACTGCATCGTGCTGTGGGAT
GGCGTGCTCGCGCTGGGAGAACTGCCCGTGCGCAGCGTGTCGTGTCAGGTCATCGAATGCCGGGACGTCGGTGGCGATAC
CTTTCGTGTGCGCCTGCGGGCACCGGCCGGCAAGCCGCCGCGCTATCACGCCGGTCAGTATTTGATGATCGAGCGCGAGA
ATGGCGAGAAATCGGCGTTCTCCATGGCTTCGGCGCCTCACGGCGGGCGCGATCTGGAAATCCATGTGCTGGCGCGCGAA
TCCAGTGCGCTGAGCCTGATCGATCAGCTCAGGCGCAACTCGATGGTGCGGGTCGAACTGCCGTTCGGCGATACCCACCT
GGCGGAACTGCCGGACGGGCCGCTGGTGCTGATCGCCGCTGGTACTGGCATGGGCCAGATCCACAGCCTGATCGAGCATT
GCCGGGCCACGGGCTTCAAACACCCGGTGCATCTGTATTGGGGCGTGCGTCGTCCGGAAGATTTTTATGAAATCGAGCAT
TGGGACGAATGGCTGAAGTTGCCCAATCTGTTCCTGCACAAAGTGGTCAGTGACCAGTGCGGCTGGGAGGGGCGCTGCGG
CATGTTGCACGAAGCGGTATGTGAGGATTTCCCTGATCTGAAATCGCTGCACGTCTATGCCAGTGGCTCTCCGGCCATGG
TCTACGGCACGCTGGATGCGCTGGTCGAAGCCGGGATGGATGCCCATCAGATGCGTGCGGATGTATTTGCTTACGCACCC
CGCTCCTGA

Upstream 100 bases:

>100_bases
GGCCGGGCGAAACCACTCGCGAGTGGGGCCGCGTCATCGTCAAGGATGACGCCGTCACCCAACGGATCGATGCCATCTGG
AATCAGTTAGGAATAGATTG

Downstream 100 bases:

>100_bases
GTTCTTTATAACTCTCCATATAACCTACCGGTATTTATGTAATTACATAAATACCGGTAGGTTATATATCCATCAGGCAA
TCAATTAAGAACTGTGCCAT

Product: CDP-6-deoxy-delta-3,4-glucoseen reductase

Products: NAD(P); FMNH2; 2-octaprenyl-6-hydroxyphenol [C]

Alternate protein names: CDP-6-deoxy-delta-3,4-glucoseen reductase; E3 [H]

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:

>322_residues
MRVTLQPSGAVLEIQPGERILDGARRLGYECPRSCRNGNCHVCAALLVEGRVEQAGKVHDHGEFYTCIAEPLEDCIVLWD
GVLALGELPVRSVSCQVIECRDVGGDTFRVRLRAPAGKPPRYHAGQYLMIERENGEKSAFSMASAPHGGRDLEIHVLARE
SSALSLIDQLRRNSMVRVELPFGDTHLAELPDGPLVLIAAGTGMGQIHSLIEHCRATGFKHPVHLYWGVRRPEDFYEIEH
WDEWLKLPNLFLHKVVSDQCGWEGRCGMLHEAVCEDFPDLKSLHVYASGSPAMVYGTLDALVEAGMDAHQMRADVFAYAP
RS

Sequences:

>Translated_322_residues
MRVTLQPSGAVLEIQPGERILDGARRLGYECPRSCRNGNCHVCAALLVEGRVEQAGKVHDHGEFYTCIAEPLEDCIVLWD
GVLALGELPVRSVSCQVIECRDVGGDTFRVRLRAPAGKPPRYHAGQYLMIERENGEKSAFSMASAPHGGRDLEIHVLARE
SSALSLIDQLRRNSMVRVELPFGDTHLAELPDGPLVLIAAGTGMGQIHSLIEHCRATGFKHPVHLYWGVRRPEDFYEIEH
WDEWLKLPNLFLHKVVSDQCGWEGRCGMLHEAVCEDFPDLKSLHVYASGSPAMVYGTLDALVEAGMDAHQMRADVFAYAP
RS
>Mature_322_residues
MRVTLQPSGAVLEIQPGERILDGARRLGYECPRSCRNGNCHVCAALLVEGRVEQAGKVHDHGEFYTCIAEPLEDCIVLWD
GVLALGELPVRSVSCQVIECRDVGGDTFRVRLRAPAGKPPRYHAGQYLMIERENGEKSAFSMASAPHGGRDLEIHVLARE
SSALSLIDQLRRNSMVRVELPFGDTHLAELPDGPLVLIAAGTGMGQIHSLIEHCRATGFKHPVHLYWGVRRPEDFYEIEH
WDEWLKLPNLFLHKVVSDQCGWEGRCGMLHEAVCEDFPDLKSLHVYASGSPAMVYGTLDALVEAGMDAHQMRADVFAYAP
RS

Specific function: Participates in the conversion of CDP-6-deoxy-D-glycero- L-threo-4-hexulose to 3,6-dideoxy-D-glycero-D-glycero-4-hexulose together with CDP-6-deoxy-D-glycero-L-threo-4-hexulose-3-dehydrase (E1) in two consecutive steps. The detailed mechanism of E3 is not

COG id: COG0543

COG function: function code HC; 2-polyprenylphenol hydroxylase and related flavodoxin oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI2367314, Length=232, Percent_Identity=28.8793103448276, Blast_Score=97, Evalue=1e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006058
- InterPro:   IPR012675
- InterPro:   IPR017927
- InterPro:   IPR001041
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR001221
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00111 Fer2; PF00175 NAD_binding_1 [H]

EC number: 1.5.1.29 [C]

Molecular weight: Translated: 35730; Mature: 35730

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: PS51085 2FE2S_FER_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.7 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
6.5 %Cys+Met (Translated Protein)
3.7 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
6.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVTLQPSGAVLEIQPGERILDGARRLGYECPRSCRNGNCHVCAALLVEGRVEQAGKVHD
CEEEECCCCCEEEECCCHHHHHHHHHCCCCCCHHHCCCCHHHHHHHHHHCHHHHCCCCCC
HGEFYTCIAEPLEDCIVLWDGVLALGELPVRSVSCQVIECRDVGGDTFRVRLRAPAGKPP
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCEEEEEEECCCCCCC
RYHAGQYLMIERENGEKSAFSMASAPHGGRDLEIHVLARESSALSLIDQLRRNSMVRVEL
CCCCCCEEEEECCCCCCHHHHHHCCCCCCCEEEEEEEECCCHHHHHHHHHHHCCEEEEEC
PFGDTHLAELPDGPLVLIAAGTGMGQIHSLIEHCRATGFKHPVHLYWGVRRPEDFYEIEH
CCCCCCHHHCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHH
WDEWLKLPNLFLHKVVSDQCGWEGRCGMLHEAVCEDFPDLKSLHVYASGSPAMVYGTLDA
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCEEEEEECCCCEEEEEEHHH
LVEAGMDAHQMRADVFAYAPRS
HHHCCCCHHHHHHHHEEECCCC
>Mature Secondary Structure
MRVTLQPSGAVLEIQPGERILDGARRLGYECPRSCRNGNCHVCAALLVEGRVEQAGKVHD
CEEEECCCCCEEEECCCHHHHHHHHHCCCCCCHHHCCCCHHHHHHHHHHCHHHHCCCCCC
HGEFYTCIAEPLEDCIVLWDGVLALGELPVRSVSCQVIECRDVGGDTFRVRLRAPAGKPP
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEECCCCCEEEEEEECCCCCCC
RYHAGQYLMIERENGEKSAFSMASAPHGGRDLEIHVLARESSALSLIDQLRRNSMVRVEL
CCCCCCEEEEECCCCCCHHHHHHCCCCCCCEEEEEEEECCCHHHHHHHHHHHCCEEEEEC
PFGDTHLAELPDGPLVLIAAGTGMGQIHSLIEHCRATGFKHPVHLYWGVRRPEDFYEIEH
CCCCCCHHHCCCCCEEEEEECCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCHHHHHHHH
WDEWLKLPNLFLHKVVSDQCGWEGRCGMLHEAVCEDFPDLKSLHVYASGSPAMVYGTLDA
HHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCEEEEEECCCCEEEEEEHHH
LVEAGMDAHQMRADVFAYAPRS
HHHCCCCHHHHHHHHEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD(P)H2; FMN [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD(P)H2; FMN; 2 2-octaprenylphenol; O2 [C]

Specific reaction: NAD(P)H2 + FMN = NAD(P) + FMNH2 2 2-octaprenylphenol + O2 = 2 2-octaprenyl-6-hydroxyphenol [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10931327; 11586360; 12142430 [H]