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The map label for this gene is gudD [H]

Identifier: 77459943

GI number: 77459943

Start: 4206647

End: 4207990

Strand: Direct

Name: gudD [H]

Synonym: Pfl01_3721

Alternate gene names: 77459943

Gene position: 4206647-4207990 (Clockwise)

Preceding gene: 77459942

Following gene: 77459945

Centisome position: 65.34

GC content: 63.39

Gene sequence:

>1344_bases
ATGACCGCACACGACATCGCCAAAGCCCCGATCATCACCGAGATGCAAGTCATCCCGGTCGCCGGCCACGACGGCATGCT
GCTCAACCTGAGCGGCGCCCACGGGCCGTTTTTCACCCGCAACATCGTCATCCTCAAGGACAACGCCGGCCACACCGGGG
TCGGTGAAGTGCCCGGCGGCGAACGTATCCGTCAGACCCTAGAAGACGCCCGCAGCCTAGTGGTCGGCAGCCCGATCGGC
ACCTACCAGAAGATCCTCAACCAGGTACGCCAGACTTTCGCCGACCGCGACGCCGGAGGCCGTGGCCTGCAAACCTTCGA
CCTGCGCATCACCATTCACGCGGTCACCGGCCTGGAAGCCGCCCTGCTCGACCTGCTCGGCCAGCACCTCGACGTGCCGG
TGGCCGCATTGCTCGGCGAAGGTCAGCAACGCGATGAAGTGAAGATGCTCGGTTATCTGTTCTACGTGGGCGATCGCCGG
GAGACCGACCTCGCATACCGCAGCGAACCGGATGCCGACAACGACTGGTTCCGCGTGCGTCACGAGAAAGCCATGACCGC
CGACGCCGTGGTCCGTCTGGCCGAGGCTGCGCACGCGCGTTATGGGTTCAAGGACTTCAAGCTCAAGGGCGGCGTACTTA
GCGGCGATGCGGAAATCGAAGCAGTGACCGCACTGGCCGAACGCTTCCCCGACGCCCGCATCACCCTTGATCCGAACGGC
GCGTGGTCACTGAAAGAAGCGATCCGTCTGTGCCGCGATCAGCATCATGTACTCGCTTACGCCGAAGACCCGTGCGGTGC
AGAAAACGGTTATTCGGGACGTGAAGTGATGGCCGAGTTCCGTCGCGCCACCGGGCTGAAAACCGCCACCAACATGATCG
CCACCGACTGGCGCGAAATGGGTCACGCAATCCAGTTGCAGTCGGTGGACATCCCGCTCGCCGACCCGCATTTCTGGACC
ATGCAGGGTTCGGTGCGCGTGGCGCAGATGTGCCACGAATGGGGCCTGACCTGGGGCTCGCACTCCAACAACCACTTCGA
CATTTCCCTGGCGATGTTCACCCACGTCGCGGCCGCCGCGCCGGGCGACATCACCGCCATCGACACCCACTGGATCTGGC
AGGACGGCCAGCGCCTGACCAAGGCACCGCTGCAAATCGTCAACGGTTGTGTGCAGATGCCGAAGAAGCCGGGGCTGGGC
GTGGAACTGGACATGGATCAGGTGGCCAAGGCCCACGAACTCTATAAAGGCATGGGGCTCGGCGCGCGGGATGACAGTGT
GGCGATGCAGTTTCTGATTCCGGGCTGGACGTTCGATAACAAGAAGCCATGTCTGGTGCGCTAG

Upstream 100 bases:

>100_bases
CCGCGATCATCGTGTTCGCCGCCCGCATGACACCCAAATCCGCCCCTGACGTCGCCGTGGCCGATGCCGCGCCGAAACAC
GCTTGAAAGGACAATCCCAA

Downstream 100 bases:

>100_bases
GCCTGCGCAACCTCCAGCAACCAGTGCCTGAACGCCGCCATCGCCGACGTCTCCGGCCGTGACTGCAAGCGCGTCAGCCA
ATAGCTGCCGGTGGTGATTT

Product: D-glucarate dehydratase

Products: NA

Alternate protein names: GDH; GlucD [H]

Number of amino acids: Translated: 447; Mature: 446

Protein sequence:

>447_residues
MTAHDIAKAPIITEMQVIPVAGHDGMLLNLSGAHGPFFTRNIVILKDNAGHTGVGEVPGGERIRQTLEDARSLVVGSPIG
TYQKILNQVRQTFADRDAGGRGLQTFDLRITIHAVTGLEAALLDLLGQHLDVPVAALLGEGQQRDEVKMLGYLFYVGDRR
ETDLAYRSEPDADNDWFRVRHEKAMTADAVVRLAEAAHARYGFKDFKLKGGVLSGDAEIEAVTALAERFPDARITLDPNG
AWSLKEAIRLCRDQHHVLAYAEDPCGAENGYSGREVMAEFRRATGLKTATNMIATDWREMGHAIQLQSVDIPLADPHFWT
MQGSVRVAQMCHEWGLTWGSHSNNHFDISLAMFTHVAAAAPGDITAIDTHWIWQDGQRLTKAPLQIVNGCVQMPKKPGLG
VELDMDQVAKAHELYKGMGLGARDDSVAMQFLIPGWTFDNKKPCLVR

Sequences:

>Translated_447_residues
MTAHDIAKAPIITEMQVIPVAGHDGMLLNLSGAHGPFFTRNIVILKDNAGHTGVGEVPGGERIRQTLEDARSLVVGSPIG
TYQKILNQVRQTFADRDAGGRGLQTFDLRITIHAVTGLEAALLDLLGQHLDVPVAALLGEGQQRDEVKMLGYLFYVGDRR
ETDLAYRSEPDADNDWFRVRHEKAMTADAVVRLAEAAHARYGFKDFKLKGGVLSGDAEIEAVTALAERFPDARITLDPNG
AWSLKEAIRLCRDQHHVLAYAEDPCGAENGYSGREVMAEFRRATGLKTATNMIATDWREMGHAIQLQSVDIPLADPHFWT
MQGSVRVAQMCHEWGLTWGSHSNNHFDISLAMFTHVAAAAPGDITAIDTHWIWQDGQRLTKAPLQIVNGCVQMPKKPGLG
VELDMDQVAKAHELYKGMGLGARDDSVAMQFLIPGWTFDNKKPCLVR
>Mature_446_residues
TAHDIAKAPIITEMQVIPVAGHDGMLLNLSGAHGPFFTRNIVILKDNAGHTGVGEVPGGERIRQTLEDARSLVVGSPIGT
YQKILNQVRQTFADRDAGGRGLQTFDLRITIHAVTGLEAALLDLLGQHLDVPVAALLGEGQQRDEVKMLGYLFYVGDRRE
TDLAYRSEPDADNDWFRVRHEKAMTADAVVRLAEAAHARYGFKDFKLKGGVLSGDAEIEAVTALAERFPDARITLDPNGA
WSLKEAIRLCRDQHHVLAYAEDPCGAENGYSGREVMAEFRRATGLKTATNMIATDWREMGHAIQLQSVDIPLADPHFWTM
QGSVRVAQMCHEWGLTWGSHSNNHFDISLAMFTHVAAAAPGDITAIDTHWIWQDGQRLTKAPLQIVNGCVQMPKKPGLGV
ELDMDQVAKAHELYKGMGLGARDDSVAMQFLIPGWTFDNKKPCLVR

Specific function: Catalyzes the dehydration of glucarate to 5-keto-4- deoxy-D-glucarate (5-kdGluc) [H]

COG id: COG4948

COG function: function code MR; L-alanine-DL-glutamate epimerase and related enzymes of enolase superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mandelate racemase/muconate lactonizing enzyme family. GlucD subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789150, Length=439, Percent_Identity=77.2209567198178, Blast_Score=726, Evalue=0.0,
Organism=Escherichia coli, GI1789151, Length=443, Percent_Identity=65.0112866817156, Blast_Score=577, Evalue=1e-166,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017653
- InterPro:   IPR013342
- InterPro:   IPR013341
- InterPro:   IPR001354 [H]

Pfam domain/function: PF01188 MR_MLE; PF02746 MR_MLE_N [H]

EC number: =4.2.1.40 [H]

Molecular weight: Translated: 49172; Mature: 49040

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAHDIAKAPIITEMQVIPVAGHDGMLLNLSGAHGPFFTRNIVILKDNAGHTGVGEVPGG
CCCHHHHCCCCEECEEEEEEECCCCEEEEECCCCCCEEEEEEEEEECCCCCCCCCCCCCH
ERIRQTLEDARSLVVGSPIGTYQKILNQVRQTFADRDAGGRGLQTFDLRITIHAVTGLEA
HHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEEEECCHHH
ALLDLLGQHLDVPVAALLGEGQQRDEVKMLGYLFYVGDRRETDLAYRSEPDADNDWFRVR
HHHHHHHHHCCCCHHHHHCCCCCCCHHHEEEEEEEECCCCCCCCCCCCCCCCCCCCEEEE
HEKAMTADAVVRLAEAAHARYGFKDFKLKGGVLSGDAEIEAVTALAERFPDARITLDPNG
HHHHHHHHHHHHHHHHHHHHCCCCCEEEECCEECCCCHHHHHHHHHHHCCCCEEEECCCC
AWSLKEAIRLCRDQHHVLAYAEDPCGAENGYSGREVMAEFRRATGLKTATNMIATDWREM
CCCHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHC
GHAIQLQSVDIPLADPHFWTMQGSVRVAQMCHEWGLTWGSHSNNHFDISLAMFTHVAAAA
CCEEEEEECCCCCCCCCEEEECCCHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHHHCC
PGDITAIDTHWIWQDGQRLTKAPLQIVNGCVQMPKKPGLGVELDMDQVAKAHELYKGMGL
CCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCEEECHHHHHHHHHHHHHCCC
GARDDSVAMQFLIPGWTFDNKKPCLVR
CCCCCCEEEEEEECCEECCCCCCCCCC
>Mature Secondary Structure 
TAHDIAKAPIITEMQVIPVAGHDGMLLNLSGAHGPFFTRNIVILKDNAGHTGVGEVPGG
CCHHHHCCCCEECEEEEEEECCCCEEEEECCCCCCEEEEEEEEEECCCCCCCCCCCCCH
ERIRQTLEDARSLVVGSPIGTYQKILNQVRQTFADRDAGGRGLQTFDLRITIHAVTGLEA
HHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEEEEECCHHH
ALLDLLGQHLDVPVAALLGEGQQRDEVKMLGYLFYVGDRRETDLAYRSEPDADNDWFRVR
HHHHHHHHHCCCCHHHHHCCCCCCCHHHEEEEEEEECCCCCCCCCCCCCCCCCCCCEEEE
HEKAMTADAVVRLAEAAHARYGFKDFKLKGGVLSGDAEIEAVTALAERFPDARITLDPNG
HHHHHHHHHHHHHHHHHHHHCCCCCEEEECCEECCCCHHHHHHHHHHHCCCCEEEECCCC
AWSLKEAIRLCRDQHHVLAYAEDPCGAENGYSGREVMAEFRRATGLKTATNMIATDWREM
CCCHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHC
GHAIQLQSVDIPLADPHFWTMQGSVRVAQMCHEWGLTWGSHSNNHFDISLAMFTHVAAAA
CCEEEEEECCCCCCCCCEEEECCCHHHHHHHHHHCCCCCCCCCCEEEEEHHHHHHHHHCC
PGDITAIDTHWIWQDGQRLTKAPLQIVNGCVQMPKKPGLGVELDMDQVAKAHELYKGMGL
CCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCEEECHHHHHHHHHHHHHCCC
GARDDSVAMQFLIPGWTFDNKKPCLVR
CCCCCCEEEEEEECCEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9772161 [H]