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The map label for this gene is paaF [C]

Identifier: 77459880

GI number: 77459880

Start: 4141127

End: 4141942

Strand: Direct

Name: paaF [C]

Synonym: Pfl01_3658

Alternate gene names: 77459880

Gene position: 4141127-4141942 (Clockwise)

Preceding gene: 77459879

Following gene: 77459881

Centisome position: 64.32

GC content: 63.6

Gene sequence:

>816_bases
ATGAGCGACTTCAACACCCTCGAATTGCACAGCGACCCACGGGGTTTCGCGACCCTGTGGCTGAGCCGCGAAGAAAAAAA
CAACGCGTTCAACGCCGAGATGATCCGCGAACTGATCCTGGCTTTGGACAAGGTCGCCAGCGACGCCAGCCTGCGTTTCC
TACTGGTGCGCGGACGCGGCAAACACTTCAGCGCCGGCGCCGATCTGGCCTGGATGCAGCAATCGGCCGAACTCGATTAC
CACACCAACCTCGACGACGCCCGGGAACTGGCGGAGCTGATGTACAACCTTGCCAAACTGAAAATCCCGACCGTGGCCGT
GGTTCAAGGCGCGGCGTTCGGTGGCGCGCTGGGCCTGATCAGTTGCTGCGACATGGCGATTGGCGCCGATGACGCGCAGT
TCTGCCTGTCGGAAGTGCGCATTGGCCTGGCGCCGGCGGTGATCAGTCCGTTCGTGGTGCAAGCCATCGGCGAACGGGCG
GCGCGGCGTTATGCGCTGACGGCCGAGCGATTCGGCGGACAGCGGGCGCGGGAAATCGGTTTGTTGTCCGAGAGCTATCC
AGCGACCGAGCTTGAACAGAAAGTCGAGCAATGGATCGACAACCTGCTGCTCAACAGCCCCGCCGCCATGCGCGCCAGCA
AGGATCTGCTGCGTGAAGTCGGCAACGGCGCGCTGACCCCGGCACTGCGTCGCTACACCGAAAATGCCATCGCCCGCATC
CGCGTCAGCCCGGAAGGCCAGGAAGGTCTGCGGGCCTTTCTGCAGAAGCGTCCGCCGAGCTGGCAAGCCGCAATCACCAC
CAAGGAGCCGCGTTGA

Upstream 100 bases:

>100_bases
CAGACCCGCGATGTATTGGCCCTGGCCTTGTCCGCGTCGTTGAACGCGCCTATCGAACCGAGCCGCTTCGGCGTGTTCCG
GATGTGATCGGGAGAACCTC

Downstream 100 bases:

>100_bases
TGAGCGCACCTGTTCTCACCACCCTGCTGGTGGCCAACCGTGGCGAAATCGCTTGCCGGGTCATGCGTACCGCCAAGGCG
CTGGGCCTGACCACCGTTGC

Product: gamma-carboxygeranoyl-CoA hydratase

Products: NA

Alternate protein names: 3-hydroxypropionyl-CoA dehydratase [H]

Number of amino acids: Translated: 271; Mature: 270

Protein sequence:

>271_residues
MSDFNTLELHSDPRGFATLWLSREEKNNAFNAEMIRELILALDKVASDASLRFLLVRGRGKHFSAGADLAWMQQSAELDY
HTNLDDARELAELMYNLAKLKIPTVAVVQGAAFGGALGLISCCDMAIGADDAQFCLSEVRIGLAPAVISPFVVQAIGERA
ARRYALTAERFGGQRAREIGLLSESYPATELEQKVEQWIDNLLLNSPAAMRASKDLLREVGNGALTPALRRYTENAIARI
RVSPEGQEGLRAFLQKRPPSWQAAITTKEPR

Sequences:

>Translated_271_residues
MSDFNTLELHSDPRGFATLWLSREEKNNAFNAEMIRELILALDKVASDASLRFLLVRGRGKHFSAGADLAWMQQSAELDY
HTNLDDARELAELMYNLAKLKIPTVAVVQGAAFGGALGLISCCDMAIGADDAQFCLSEVRIGLAPAVISPFVVQAIGERA
ARRYALTAERFGGQRAREIGLLSESYPATELEQKVEQWIDNLLLNSPAAMRASKDLLREVGNGALTPALRRYTENAIARI
RVSPEGQEGLRAFLQKRPPSWQAAITTKEPR
>Mature_270_residues
SDFNTLELHSDPRGFATLWLSREEKNNAFNAEMIRELILALDKVASDASLRFLLVRGRGKHFSAGADLAWMQQSAELDYH
TNLDDARELAELMYNLAKLKIPTVAVVQGAAFGGALGLISCCDMAIGADDAQFCLSEVRIGLAPAVISPFVVQAIGERAA
RRYALTAERFGGQRAREIGLLSESYPATELEQKVEQWIDNLLLNSPAAMRASKDLLREVGNGALTPALRRYTENAIARIR
VSPEGQEGLRAFLQKRPPSWQAAITTKEPR

Specific function: Plays a role in autotrophic carbon fixation via the 3- hydroxypropionate/4-hydroxybutyrate cycle. Catalyzes the reversible dehydration of 3-hydroxypropionyl-CoA to form acryloyl- CoA, and the reversible dehydration of (S)-3-hydroxybutyryl-CoA to form crot

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI31542718, Length=252, Percent_Identity=26.1904761904762, Blast_Score=92, Evalue=7e-19,
Organism=Homo sapiens, GI194097323, Length=245, Percent_Identity=26.9387755102041, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI70995211, Length=200, Percent_Identity=28.5, Blast_Score=79, Evalue=6e-15,
Organism=Homo sapiens, GI4502327, Length=272, Percent_Identity=26.8382352941176, Blast_Score=72, Evalue=6e-13,
Organism=Homo sapiens, GI260274832, Length=223, Percent_Identity=26.457399103139, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI45643119, Length=221, Percent_Identity=26.6968325791855, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI260275230, Length=221, Percent_Identity=26.6968325791855, Blast_Score=70, Evalue=1e-12,
Organism=Homo sapiens, GI221136756, Length=249, Percent_Identity=25.7028112449799, Blast_Score=68, Evalue=9e-12,
Organism=Homo sapiens, GI221136753, Length=249, Percent_Identity=25.7028112449799, Blast_Score=68, Evalue=9e-12,
Organism=Homo sapiens, GI221307494, Length=249, Percent_Identity=25.7028112449799, Blast_Score=68, Evalue=1e-11,
Organism=Homo sapiens, GI157694516, Length=217, Percent_Identity=23.5023041474654, Blast_Score=64, Evalue=1e-10,
Organism=Homo sapiens, GI213417737, Length=217, Percent_Identity=23.5023041474654, Blast_Score=64, Evalue=1e-10,
Organism=Escherichia coli, GI1787659, Length=245, Percent_Identity=29.7959183673469, Blast_Score=110, Evalue=9e-26,
Organism=Escherichia coli, GI1788597, Length=264, Percent_Identity=27.2727272727273, Blast_Score=101, Evalue=4e-23,
Organism=Escherichia coli, GI221142681, Length=252, Percent_Identity=26.984126984127, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI87082183, Length=249, Percent_Identity=24.8995983935743, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1787660, Length=257, Percent_Identity=28.7937743190661, Blast_Score=68, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI25145438, Length=257, Percent_Identity=28.4046692607004, Blast_Score=93, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17554946, Length=264, Percent_Identity=25.7575757575758, Blast_Score=83, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17549921, Length=206, Percent_Identity=27.6699029126214, Blast_Score=81, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI17536985, Length=213, Percent_Identity=27.6995305164319, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17540306, Length=243, Percent_Identity=21.8106995884774, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17535521, Length=243, Percent_Identity=24.2798353909465, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17534483, Length=210, Percent_Identity=25.2380952380952, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI20129971, Length=262, Percent_Identity=24.0458015267176, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24653477, Length=262, Percent_Identity=24.0458015267176, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24653139, Length=266, Percent_Identity=25.187969924812, Blast_Score=80, Evalue=1e-15,
Organism=Drosophila melanogaster, GI19922422, Length=256, Percent_Identity=20.703125, Blast_Score=72, Evalue=3e-13,
Organism=Drosophila melanogaster, GI19920382, Length=227, Percent_Identity=28.6343612334802, Blast_Score=70, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014748
- InterPro:   IPR001753
- InterPro:   IPR018376 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.116 [H]

Molecular weight: Translated: 29842; Mature: 29711

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDFNTLELHSDPRGFATLWLSREEKNNAFNAEMIRELILALDKVASDASLRFLLVRGRG
CCCCCEEEECCCCCCEEEEEEECHHCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCC
KHFSAGADLAWMQQSAELDYHTNLDDARELAELMYNLAKLKIPTVAVVQGAAFGGALGLI
CCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHH
SCCDMAIGADDAQFCLSEVRIGLAPAVISPFVVQAIGERAARRYALTAERFGGQRAREIG
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
LLSESYPATELEQKVEQWIDNLLLNSPAAMRASKDLLREVGNGALTPALRRYTENAIARI
CCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHEEE
RVSPEGQEGLRAFLQKRPPSWQAAITTKEPR
EECCCCHHHHHHHHHCCCCCCEEEEECCCCC
>Mature Secondary Structure 
SDFNTLELHSDPRGFATLWLSREEKNNAFNAEMIRELILALDKVASDASLRFLLVRGRG
CCCCEEEECCCCCCEEEEEEECHHCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEEECCC
KHFSAGADLAWMQQSAELDYHTNLDDARELAELMYNLAKLKIPTVAVVQGAAFGGALGLI
CCCCCCCCHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHH
SCCDMAIGADDAQFCLSEVRIGLAPAVISPFVVQAIGERAARRYALTAERFGGQRAREIG
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
LLSESYPATELEQKVEQWIDNLLLNSPAAMRASKDLLREVGNGALTPALRRYTENAIARI
CCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHEEE
RVSPEGQEGLRAFLQKRPPSWQAAITTKEPR
EECCCCHHHHHHHHHCCCCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA