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The map label for this gene is mvaB [H]

Identifier: 77459876

GI number: 77459876

Start: 4134938

End: 4135837

Strand: Direct

Name: mvaB [H]

Synonym: Pfl01_3654

Alternate gene names: 77459876

Gene position: 4134938-4135837 (Clockwise)

Preceding gene: 77459875

Following gene: 77459878

Centisome position: 64.22

GC content: 64.78

Gene sequence:

>900_bases
ATGTCCCTCCCCTCCCAAGTACGCCTGATCGAAGTCGGCCCGCGTGACGGCCTGCAGAACGAAGCCCAGCCCATCAGCGT
CGCCGACAAGGTGCAACTGGTCGACGCGCTGAGCGCCGCCGGTCTCGGCTATATAGAAGTCGGCAGTTTCGTCTCGCCCA
AGTGGGTGCCGCAGATGGCCGGTTCCGCCGAGGTGTTCGCGCAGATCCAGCGCAAGCCCGGCGTAACCTATGGTGCATTG
GCGCCGAACCTGCGCGGTTTTGAAGATGCACTTGCGGCGGGAGTGAAGGAAGTCGCGGTGTTTGCCGCTGCGTCCGAAGC
GTTTTCGCAGCGCAACATCAATTGCTCGATCAGCGAAAGCCTGGCGCGGTTCGCGCCGATCATGGAATCGGCGAAACAGC
ACGGCGTTACCGTGCGCGGTTACGTGTCCTGCGTGTTGGGCTGTCCTTATGAAGGTGAAGTCGCCCCGGAGCAAGTGGCG
ATGGTGGCCCGCGAGCTGTACGCGATGGGCTGCTATGAAGTGTCGCTGGGTGACACCATCGGTACCGGCACCGCGGGCGC
GACCCGCCGGCTGTTCGAAGTGGTCTCGAAACAGGTGCCACGCGAGAAACTCGCCGGCCACTTCCACGACACCTACGGCC
AGGCCATGGCCAACATCTATGCAAGCCTGCTGGAAGGCATCGCGGTGTTCGACAGCTCGATCGCCGGCCTCGGCGGCTGC
CCGTACGCCAAGGGCGCCAGCGGTAACGTCGCCACCGAAGACGTGGTGTACCTGCTCAACGGCCTGGGCATCGAGACCGG
TATCGACCTCGACGCCTTGATTGCCGCCGGCCAGCAGATCAGCTCCGTGCTGGGCCGCCCGACCGGTTCGCGCGTGGCCA
AGGCTCGCAGCGCACAGTGA

Upstream 100 bases:

>100_bases
GCAGGCGCTGGAGCAGACGCTCAAGAGTCAGCAATCAGTTCAGTAACCTAAATAAATCCCACACGGGGTTCCGCAGGAAT
TCAAAGAGCAGGTCAACACC

Downstream 100 bases:

>100_bases
GGGATGGTGTTACCGCCGAGCGCGAAACGTGGGCACAAGCGAGTAACACGGAAACAAATTGTCTGGTTTTGCCTGAAGGA
AAAATCCTTCAAAAATCTCA

Product: hydroxymethylglutaryl-CoA lyase

Products: NA

Alternate protein names: HL; HMG-CoA lyase; 3-hydroxy-3-methylglutarate-CoA lyase [H]

Number of amino acids: Translated: 299; Mature: 298

Protein sequence:

>299_residues
MSLPSQVRLIEVGPRDGLQNEAQPISVADKVQLVDALSAAGLGYIEVGSFVSPKWVPQMAGSAEVFAQIQRKPGVTYGAL
APNLRGFEDALAAGVKEVAVFAAASEAFSQRNINCSISESLARFAPIMESAKQHGVTVRGYVSCVLGCPYEGEVAPEQVA
MVARELYAMGCYEVSLGDTIGTGTAGATRRLFEVVSKQVPREKLAGHFHDTYGQAMANIYASLLEGIAVFDSSIAGLGGC
PYAKGASGNVATEDVVYLLNGLGIETGIDLDALIAAGQQISSVLGRPTGSRVAKARSAQ

Sequences:

>Translated_299_residues
MSLPSQVRLIEVGPRDGLQNEAQPISVADKVQLVDALSAAGLGYIEVGSFVSPKWVPQMAGSAEVFAQIQRKPGVTYGAL
APNLRGFEDALAAGVKEVAVFAAASEAFSQRNINCSISESLARFAPIMESAKQHGVTVRGYVSCVLGCPYEGEVAPEQVA
MVARELYAMGCYEVSLGDTIGTGTAGATRRLFEVVSKQVPREKLAGHFHDTYGQAMANIYASLLEGIAVFDSSIAGLGGC
PYAKGASGNVATEDVVYLLNGLGIETGIDLDALIAAGQQISSVLGRPTGSRVAKARSAQ
>Mature_298_residues
SLPSQVRLIEVGPRDGLQNEAQPISVADKVQLVDALSAAGLGYIEVGSFVSPKWVPQMAGSAEVFAQIQRKPGVTYGALA
PNLRGFEDALAAGVKEVAVFAAASEAFSQRNINCSISESLARFAPIMESAKQHGVTVRGYVSCVLGCPYEGEVAPEQVAM
VARELYAMGCYEVSLGDTIGTGTAGATRRLFEVVSKQVPREKLAGHFHDTYGQAMANIYASLLEGIAVFDSSIAGLGGCP
YAKGASGNVATEDVVYLLNGLGIETGIDLDALIAAGQQISSVLGRPTGSRVAKARSAQ

Specific function: Involved in the catabolism of branched amino acids such as leucine (Probable) [H]

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HMG-CoA lyase family [H]

Homologues:

Organism=Homo sapiens, GI62198232, Length=294, Percent_Identity=57.4829931972789, Blast_Score=368, Evalue=1e-102,
Organism=Homo sapiens, GI109150422, Length=293, Percent_Identity=55.2901023890785, Blast_Score=352, Evalue=2e-97,
Organism=Homo sapiens, GI109150427, Length=293, Percent_Identity=55.2901023890785, Blast_Score=352, Evalue=2e-97,
Organism=Homo sapiens, GI260654708, Length=136, Percent_Identity=57.3529411764706, Blast_Score=166, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI17510563, Length=296, Percent_Identity=51.0135135135135, Blast_Score=309, Evalue=1e-84,
Organism=Drosophila melanogaster, GI24582381, Length=294, Percent_Identity=58.843537414966, Blast_Score=365, Evalue=1e-101,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR000138
- InterPro:   IPR000891 [H]

Pfam domain/function: PF00682 HMGL-like [H]

EC number: =4.1.3.4 [H]

Molecular weight: Translated: 31159; Mature: 31028

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLPSQVRLIEVGPRDGLQNEAQPISVADKVQLVDALSAAGLGYIEVGSFVSPKWVPQMA
CCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEECCCCCCCCHHHHHC
GSAEVFAQIQRKPGVTYGALAPNLRGFEDALAAGVKEVAVFAAASEAFSQRNINCSISES
CCHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
LARFAPIMESAKQHGVTVRGYVSCVLGCPYEGEVAPEQVAMVARELYAMGCYEVSLGDTI
HHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCEEEECCCCC
GTGTAGATRRLFEVVSKQVPREKLAGHFHDTYGQAMANIYASLLEGIAVFDSSIAGLGGC
CCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PYAKGASGNVATEDVVYLLNGLGIETGIDLDALIAAGQQISSVLGRPTGSRVAKARSAQ
CCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHCCCC
>Mature Secondary Structure 
SLPSQVRLIEVGPRDGLQNEAQPISVADKVQLVDALSAAGLGYIEVGSFVSPKWVPQMA
CCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEECCCCCCCCHHHHHC
GSAEVFAQIQRKPGVTYGALAPNLRGFEDALAAGVKEVAVFAAASEAFSQRNINCSISES
CCHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
LARFAPIMESAKQHGVTVRGYVSCVLGCPYEGEVAPEQVAMVARELYAMGCYEVSLGDTI
HHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCEEEECCCCC
GTGTAGATRRLFEVVSKQVPREKLAGHFHDTYGQAMANIYASLLEGIAVFDSSIAGLGGC
CCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
PYAKGASGNVATEDVVYLLNGLGIETGIDLDALIAAGQQISSVLGRPTGSRVAKARSAQ
CCCCCCCCCCHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2687236; 2656635; 1637819 [H]