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The map label for this gene is emrA [H]

Identifier: 77459865

GI number: 77459865

Start: 4119923

End: 4121125

Strand: Reverse

Name: emrA [H]

Synonym: Pfl01_3643

Alternate gene names: 77459865

Gene position: 4121125-4119923 (Counterclockwise)

Preceding gene: 77459866

Following gene: 77459864

Centisome position: 64.01

GC content: 63.09

Gene sequence:

>1203_bases
ATGGCCACTGCCGAAAACACACAAGCTCAAGACAACGCCCAGGACACTGGCAATCCGCGCAAACGCAAGGTGATGCTGCT
GGTGCTGGCCGTTGTGGTTGCCCTCGCCGGTGTCGGCGTCTGGGCGTATCACGAATTCATCGGGCGCTGGAGCGAAAGCA
CCGACGACGCCTACGTCAACGGCAACGTGGTGGAAATCACCCCGCTGGTGACCGGCACCGTGGTCAGCATCGGTGCTGAC
GATGGCGATCTGGTGCATGAAGGCCAGGTGCTGATCAACTTCGACCCGAATGACGCCGAAGTCGGCCTGCAAAGTGCCAA
GGCCAATCTGGCCCGCACCGTGCGTCAGGTGCGCGGCCTGTACAGCAACGTCGACGGCATGAAGGCCCAGGTCAATGCGC
AACAGGCCGAAGTGCAGAAAGCCCAGGACAACTTCAACCGCCGGAAAAACCTCGCGGCCGGCGGGGCGATTTCCCAGGAA
GAACTGTCCCACGCCCGCGATGACCTGACCTCGGCGCAGAACGCCCTGGCCAACGCCAAACAGCAACTGAAAACCACCAG
CGCGCTGGTCGATGACACCGTGGTGTCCTCGCACCCGGACGTGATGGCCGCCGCCGCGCAACTGCGTCAGGCCTACCTGA
CCAACGCTCGCAGCACCTTGATTGCGCCGGTCACCGGTTATGTCGCCAAACGCACCGTGCAACTTGGCCAGCGTGTGCAG
CCGGGCACCGCGCTGATGGCGGTGATTCCGCTGGATCAGCTGTGGATCGACGCCAACTTCAAGGAAACCCAGCTGCGTGA
CATGCGCATTGGCCAACCGGTGGAGATCGAGTCGGACATCTACGGCAGTGACGTCAAGTTCAACGGCACCGTCGACAGCC
TCGGCGCGGGCACCGGCAGCGCGTTCGCCCTGTTGCCGGCGCAGAACGCCACCGGTAACTGGATCAAGATCGTGCAGCGT
GTGCCGGTACGGATTCACGTCAACGCCGAAGAGCTGGCCAAACACCCGCTTCGCGTCGGCCTGTCGACCAATGTCGAGGT
CAATCTGCACGACCAGAGCGGCCCGGTACTGGCCCAGCAACCGCCGCAAAAGGCCTCGTTCAGCACCAACGTCTACGATC
GCCAACTGGCCGAAGCCGACGCGATGATCACCCAGTTGATCCACGACAACAGCGCGGCGGTCAGCAAGACTGCGCAACGC
TGA

Upstream 100 bases:

>100_bases
TCAACTGATGCAAGCGCTGGGCGGCGGCTTCCAGGGGCAAACCCTGACCGCAGCCAACGCCACCCCAGCCACGCCGCACA
ACTAATTCAAGGTACTTGTC

Downstream 100 bases:

>100_bases
TCTCTTTCCCCCTGTGGGAGCGGGCTTGCTCCCACAAAGGGCAGCTGTGTCCGTAACAGACACGGCGCCAACCCGGCTTC
ATAGGATTCGCGATGAGCAA

Product: secretion protein HlyD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 400; Mature: 399

Protein sequence:

>400_residues
MATAENTQAQDNAQDTGNPRKRKVMLLVLAVVVALAGVGVWAYHEFIGRWSESTDDAYVNGNVVEITPLVTGTVVSIGAD
DGDLVHEGQVLINFDPNDAEVGLQSAKANLARTVRQVRGLYSNVDGMKAQVNAQQAEVQKAQDNFNRRKNLAAGGAISQE
ELSHARDDLTSAQNALANAKQQLKTTSALVDDTVVSSHPDVMAAAAQLRQAYLTNARSTLIAPVTGYVAKRTVQLGQRVQ
PGTALMAVIPLDQLWIDANFKETQLRDMRIGQPVEIESDIYGSDVKFNGTVDSLGAGTGSAFALLPAQNATGNWIKIVQR
VPVRIHVNAEELAKHPLRVGLSTNVEVNLHDQSGPVLAQQPPQKASFSTNVYDRQLAEADAMITQLIHDNSAAVSKTAQR

Sequences:

>Translated_400_residues
MATAENTQAQDNAQDTGNPRKRKVMLLVLAVVVALAGVGVWAYHEFIGRWSESTDDAYVNGNVVEITPLVTGTVVSIGAD
DGDLVHEGQVLINFDPNDAEVGLQSAKANLARTVRQVRGLYSNVDGMKAQVNAQQAEVQKAQDNFNRRKNLAAGGAISQE
ELSHARDDLTSAQNALANAKQQLKTTSALVDDTVVSSHPDVMAAAAQLRQAYLTNARSTLIAPVTGYVAKRTVQLGQRVQ
PGTALMAVIPLDQLWIDANFKETQLRDMRIGQPVEIESDIYGSDVKFNGTVDSLGAGTGSAFALLPAQNATGNWIKIVQR
VPVRIHVNAEELAKHPLRVGLSTNVEVNLHDQSGPVLAQQPPQKASFSTNVYDRQLAEADAMITQLIHDNSAAVSKTAQR
>Mature_399_residues
ATAENTQAQDNAQDTGNPRKRKVMLLVLAVVVALAGVGVWAYHEFIGRWSESTDDAYVNGNVVEITPLVTGTVVSIGADD
GDLVHEGQVLINFDPNDAEVGLQSAKANLARTVRQVRGLYSNVDGMKAQVNAQQAEVQKAQDNFNRRKNLAAGGAISQEE
LSHARDDLTSAQNALANAKQQLKTTSALVDDTVVSSHPDVMAAAAQLRQAYLTNARSTLIAPVTGYVAKRTVQLGQRVQP
GTALMAVIPLDQLWIDANFKETQLRDMRIGQPVEIESDIYGSDVKFNGTVDSLGAGTGSAFALLPAQNATGNWIKIVQRV
PVRIHVNAEELAKHPLRVGLSTNVEVNLHDQSGPVLAQQPPQKASFSTNVYDRQLAEADAMITQLIHDNSAAVSKTAQR

Specific function: The emr locus confers resistance to substances of high hydrophobicity. EmrA probably participate in a transport system to extrude toxins and drugs from the cell [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789041, Length=390, Percent_Identity=43.8461538461538, Blast_Score=317, Evalue=9e-88,
Organism=Escherichia coli, GI1788711, Length=340, Percent_Identity=44.7058823529412, Blast_Score=293, Evalue=1e-80,
Organism=Escherichia coli, GI1790519, Length=304, Percent_Identity=28.9473684210526, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI87081951, Length=307, Percent_Identity=28.0130293159609, Blast_Score=103, Evalue=2e-23,
Organism=Escherichia coli, GI1789637, Length=317, Percent_Identity=25.5520504731861, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1790012, Length=221, Percent_Identity=23.5294117647059, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1790024, Length=237, Percent_Identity=24.8945147679325, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005694
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 42983; Mature: 42852

Theoretical pI: Translated: 5.88; Mature: 5.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATAENTQAQDNAQDTGNPRKRKVMLLVLAVVVALAGVGVWAYHEFIGRWSESTDDAYVN
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCEEEC
GNVVEITPLVTGTVVSIGADDGDLVHEGQVLINFDPNDAEVGLQSAKANLARTVRQVRGL
CCEEEEEEEEEEEEEEECCCCCCEEECCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHH
YSNVDGMKAQVNAQQAEVQKAQDNFNRRKNLAAGGAISQEELSHARDDLTSAQNALANAK
HHCCCCCEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
QQLKTTSALVDDTVVSSHPDVMAAAAQLRQAYLTNARSTLIAPVTGYVAKRTVQLGQRVQ
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHEEHHHHHHHHHHHHHHCCCCC
PGTALMAVIPLDQLWIDANFKETQLRDMRIGQPVEIESDIYGSDVKFNGTVDSLGAGTGS
CCCEEEEEEEHHHEEECCCCCHHHHHHCCCCCCCEECCCCCCCCEEECCCHHHCCCCCCC
AFALLPAQNATGNWIKIVQRVPVRIHVNAEELAKHPLRVGLSTNVEVNLHDQSGPVLAQQ
EEEEEECCCCCCHHHHHHHHCCEEEEECHHHHHCCCEEECCCCCEEEEEECCCCCCCCCC
PPQKASFSTNVYDRQLAEADAMITQLIHDNSAAVSKTAQR
CCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHCCC
>Mature Secondary Structure 
ATAENTQAQDNAQDTGNPRKRKVMLLVLAVVVALAGVGVWAYHEFIGRWSESTDDAYVN
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCCEEEC
GNVVEITPLVTGTVVSIGADDGDLVHEGQVLINFDPNDAEVGLQSAKANLARTVRQVRGL
CCEEEEEEEEEEEEEEECCCCCCEEECCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHH
YSNVDGMKAQVNAQQAEVQKAQDNFNRRKNLAAGGAISQEELSHARDDLTSAQNALANAK
HHCCCCCEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
QQLKTTSALVDDTVVSSHPDVMAAAAQLRQAYLTNARSTLIAPVTGYVAKRTVQLGQRVQ
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHEEHHHHHHHHHHHHHHCCCCC
PGTALMAVIPLDQLWIDANFKETQLRDMRIGQPVEIESDIYGSDVKFNGTVDSLGAGTGS
CCCEEEEEEEHHHEEECCCCCHHHHHHCCCCCCCEECCCCCCCCEEECCCHHHCCCCCCC
AFALLPAQNATGNWIKIVQRVPVRIHVNAEELAKHPLRVGLSTNVEVNLHDQSGPVLAQQ
EEEEEECCCCCCHHHHHHHHCCEEEEECHHHHHCCCEEECCCCCEEEEEECCCCCCCCCC
PPQKASFSTNVYDRQLAEADAMITQLIHDNSAAVSKTAQR
CCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1409590; 9205837; 9278503; 8203018 [H]