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The map label for this gene is emrB [H]

Identifier: 77459864

GI number: 77459864

Start: 4118301

End: 4119830

Strand: Reverse

Name: emrB [H]

Synonym: Pfl01_3642

Alternate gene names: 77459864

Gene position: 4119830-4118301 (Counterclockwise)

Preceding gene: 77459865

Following gene: 77459861

Centisome position: 63.99

GC content: 61.44

Gene sequence:

>1530_bases
ATGAGCAATAACGCCTCTTTCACGCCGCCCAGCCTGTTGCTCAGCACCATCGGCCTGTCGCTGGCGACCTTCATGCAGGT
GCTCGACACCACGATCGCCAACGTGGCGCTGCCAACCATTTCCGGCAACCTCGGCGTGAGTTCGGAGCAGGGCACCTGGG
TCATCACCTCGTTCGCCGTGAGCAACGCCATTGCGCTGCCGCTCACCGGCTGGCTGAGCCGTCGCTTCGGCGAGGTGAAG
CTGTTTCTGTGGGCGACCATCCTGTTTGTGCTGGCTTCGTTCCTCTGCGGTATTTCCACATCGATGCCGGAACTGATCGG
CTTCCGCGTGCTGCAAGGCCTGGTGGCCGGGCCGTTGTACCCGATGACCCAGACGCTGCTGATCGCGGTCTATCCACCGG
CGAGGAGGGGCATGGCCCTGGCGTTGCTGGCGATGGTCACGGTGGTGGCGCCGATTGCCGGGCCGATCCTCGGCGGCTGG
ATCACCGACAGTTACAGCTGGCCGTGGATCTTCTTCATCAACGTGCCGATCGGGATTTTTGCGGTGATGGTGGTGCGCCA
GCAGCTGGCCAAACGCCCGGTGGTCACCAGCCGTCAGCCGATGGATTACGTCGGGCTGATCACGCTGATCATCGGCGTGG
GTGCGTTGCAGGTGATCCTCGACAAGGGCAATGACCTGGACTGGTTCGAGTCAAACTTCATCATCATCGGCGCGGCGATT
TCGGTGATCGCGCTGGCGGTGTTCGTGATATGGGAAATGACCGACCAGCATCCGGTAGTCAATTTGCGCCTGTTCGCCTA
TCGCAACTTCCGCATCGGTACGCTGGTGCTGGTATTGGGTTACGCCGGGTTCTTCGGCATCAACCTGATCCTGCCGCAAT
GGTTGCAGACCCAGATGGGCTACACCGCGACCTGGGCCGGACTGGCGGTGGCACCGATCGGCATTCTGCCGGTGCTGCTG
TCGCCGTTTGTCGGCAAGTATGCGCACAAGTTCGACCTACGTCTGTTGGCCGGTCTGGCGTTCCTGGCGATCGGCCTGAG
CTGCTTCATGCGTGCGGAGTTCACCAACGAGGTGGACTTCCAGCACATCGCTCTGGTGCAGTTGTTCATGGGGATTGGCG
TGGCGCTGTTCTTCATGCCGACCCTGAGCATTCTGATGTCGGACTTGCCGCCGAACCAGATTGCCGATGGTGCGGGTCTG
GCGACGTTCCTGCGGACATTGGGTGGCAGTTTTGCGGCGTCGTTGACCACGTGGATCTGGATTCGCCGGGCGGATCAGCA
TCATGCCTACATGAGCGAGAGTATCAGCACCTTCGAACCAGCGACTCGCGAGACCTTGCATTCGCTGGGTGGCGCGAGCC
AATCGGCCTATGCGCAGATGGATCAGATCCTCACCAGTCAGGCGTACATGCTCTCCACCGTGGATTACTTCACGCTGCTG
GGCTGGGGGTTCATGGGGTTGATTCTGATTGTGTGGCTGGCCAAGCCGCCATTCGCAGCCAAGGCAGGGCCGGCGGCTTC
CGGGCACTAA

Upstream 100 bases:

>100_bases
AACGCTGATCTCTTTCCCCCTGTGGGAGCGGGCTTGCTCCCACAAAGGGCAGCTGTGTCCGTAACAGACACGGCGCCAAC
CCGGCTTCATAGGATTCGCG

Downstream 100 bases:

>100_bases
GATCAAGAGCTTTCCCTCACCCTAACCCTCTCCCGGAGGGAGAGGGGACTGACCGAGGTGGATATTCGGGTTACACCGAC
CTGAAAGTCCGAGTTGAACT

Product: EmrB/QacA family drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 509; Mature: 508

Protein sequence:

>509_residues
MSNNASFTPPSLLLSTIGLSLATFMQVLDTTIANVALPTISGNLGVSSEQGTWVITSFAVSNAIALPLTGWLSRRFGEVK
LFLWATILFVLASFLCGISTSMPELIGFRVLQGLVAGPLYPMTQTLLIAVYPPARRGMALALLAMVTVVAPIAGPILGGW
ITDSYSWPWIFFINVPIGIFAVMVVRQQLAKRPVVTSRQPMDYVGLITLIIGVGALQVILDKGNDLDWFESNFIIIGAAI
SVIALAVFVIWEMTDQHPVVNLRLFAYRNFRIGTLVLVLGYAGFFGINLILPQWLQTQMGYTATWAGLAVAPIGILPVLL
SPFVGKYAHKFDLRLLAGLAFLAIGLSCFMRAEFTNEVDFQHIALVQLFMGIGVALFFMPTLSILMSDLPPNQIADGAGL
ATFLRTLGGSFAASLTTWIWIRRADQHHAYMSESISTFEPATRETLHSLGGASQSAYAQMDQILTSQAYMLSTVDYFTLL
GWGFMGLILIVWLAKPPFAAKAGPAASGH

Sequences:

>Translated_509_residues
MSNNASFTPPSLLLSTIGLSLATFMQVLDTTIANVALPTISGNLGVSSEQGTWVITSFAVSNAIALPLTGWLSRRFGEVK
LFLWATILFVLASFLCGISTSMPELIGFRVLQGLVAGPLYPMTQTLLIAVYPPARRGMALALLAMVTVVAPIAGPILGGW
ITDSYSWPWIFFINVPIGIFAVMVVRQQLAKRPVVTSRQPMDYVGLITLIIGVGALQVILDKGNDLDWFESNFIIIGAAI
SVIALAVFVIWEMTDQHPVVNLRLFAYRNFRIGTLVLVLGYAGFFGINLILPQWLQTQMGYTATWAGLAVAPIGILPVLL
SPFVGKYAHKFDLRLLAGLAFLAIGLSCFMRAEFTNEVDFQHIALVQLFMGIGVALFFMPTLSILMSDLPPNQIADGAGL
ATFLRTLGGSFAASLTTWIWIRRADQHHAYMSESISTFEPATRETLHSLGGASQSAYAQMDQILTSQAYMLSTVDYFTLL
GWGFMGLILIVWLAKPPFAAKAGPAASGH
>Mature_508_residues
SNNASFTPPSLLLSTIGLSLATFMQVLDTTIANVALPTISGNLGVSSEQGTWVITSFAVSNAIALPLTGWLSRRFGEVKL
FLWATILFVLASFLCGISTSMPELIGFRVLQGLVAGPLYPMTQTLLIAVYPPARRGMALALLAMVTVVAPIAGPILGGWI
TDSYSWPWIFFINVPIGIFAVMVVRQQLAKRPVVTSRQPMDYVGLITLIIGVGALQVILDKGNDLDWFESNFIIIGAAIS
VIALAVFVIWEMTDQHPVVNLRLFAYRNFRIGTLVLVLGYAGFFGINLILPQWLQTQMGYTATWAGLAVAPIGILPVLLS
PFVGKYAHKFDLRLLAGLAFLAIGLSCFMRAEFTNEVDFQHIALVQLFMGIGVALFFMPTLSILMSDLPPNQIADGAGLA
TFLRTLGGSFAASLTTWIWIRRADQHHAYMSESISTFEPATRETLHSLGGASQSAYAQMDQILTSQAYMLSTVDYFTLLG
WGFMGLILIVWLAKPPFAAKAGPAASGH

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=502, Percent_Identity=50.796812749004, Blast_Score=495, Evalue=1e-141,
Organism=Escherichia coli, GI1788710, Length=505, Percent_Identity=46.5346534653465, Blast_Score=446, Evalue=1e-126,
Organism=Escherichia coli, GI1790195, Length=387, Percent_Identity=30.2325581395349, Blast_Score=154, Evalue=9e-39,
Organism=Escherichia coli, GI1788392, Length=421, Percent_Identity=27.790973871734, Blast_Score=119, Evalue=4e-28,
Organism=Escherichia coli, GI87081983, Length=419, Percent_Identity=24.1050119331742, Blast_Score=114, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6325455, Length=144, Percent_Identity=31.25, Blast_Score=80, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6321663, Length=150, Percent_Identity=28.6666666666667, Blast_Score=77, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6322958, Length=198, Percent_Identity=27.2727272727273, Blast_Score=76, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 55194; Mature: 55063

Theoretical pI: Translated: 7.69; Mature: 7.69

Prosite motif: PS00107 PROTEIN_KINASE_ATP ; PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNNASFTPPSLLLSTIGLSLATFMQVLDTTIANVALPTISGNLGVSSEQGTWVITSFAV
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEHHH
SNAIALPLTGWLSRRFGEVKLFLWATILFVLASFLCGISTSMPELIGFRVLQGLVAGPLY
HCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCH
PMTQTLLIAVYPPARRGMALALLAMVTVVAPIAGPILGGWITDSYSWPWIFFINVPIGIF
HHHHEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCHHHH
AVMVVRQQLAKRPVVTSRQPMDYVGLITLIIGVGALQVILDKGNDLDWFESNFIIIGAAI
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEHHHH
SVIALAVFVIWEMTDQHPVVNLRLFAYRNFRIGTLVLVLGYAGFFGINLILPQWLQTQMG
HHHHHHHHHHHHHCCCCCEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
YTATWAGLAVAPIGILPVLLSPFVGKYAHKFDLRLLAGLAFLAIGLSCFMRAEFTNEVDF
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
QHIALVQLFMGIGVALFFMPTLSILMSDLPPNQIADGAGLATFLRTLGGSFAASLTTWIW
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHCCHHHHHHHHHHH
IRRADQHHAYMSESISTFEPATRETLHSLGGASQSAYAQMDQILTSQAYMLSTVDYFTLL
HEECCHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
GWGFMGLILIVWLAKPPFAAKAGPAASGH
HHHHHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SNNASFTPPSLLLSTIGLSLATFMQVLDTTIANVALPTISGNLGVSSEQGTWVITSFAV
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEHHH
SNAIALPLTGWLSRRFGEVKLFLWATILFVLASFLCGISTSMPELIGFRVLQGLVAGPLY
HCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCH
PMTQTLLIAVYPPARRGMALALLAMVTVVAPIAGPILGGWITDSYSWPWIFFINVPIGIF
HHHHEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCHHHH
AVMVVRQQLAKRPVVTSRQPMDYVGLITLIIGVGALQVILDKGNDLDWFESNFIIIGAAI
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEHHHH
SVIALAVFVIWEMTDQHPVVNLRLFAYRNFRIGTLVLVLGYAGFFGINLILPQWLQTQMG
HHHHHHHHHHHHHCCCCCEEEEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
YTATWAGLAVAPIGILPVLLSPFVGKYAHKFDLRLLAGLAFLAIGLSCFMRAEFTNEVDF
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCH
QHIALVQLFMGIGVALFFMPTLSILMSDLPPNQIADGAGLATFLRTLGGSFAASLTTWIW
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHCCHHHHHHHHHHH
IRRADQHHAYMSESISTFEPATRETLHSLGGASQSAYAQMDQILTSQAYMLSTVDYFTLL
HEECCHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
GWGFMGLILIVWLAKPPFAAKAGPAASGH
HHHHHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]