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The map label for this gene is irlS [H]

Identifier: 77459847

GI number: 77459847

Start: 4098761

End: 4100170

Strand: Direct

Name: irlS [H]

Synonym: Pfl01_3625

Alternate gene names: 77459847

Gene position: 4098761-4100170 (Clockwise)

Preceding gene: 77459846

Following gene: 77459848

Centisome position: 63.66

GC content: 61.06

Gene sequence:

>1410_bases
ATGAAGCCGGCCAGCCTGTCGCTACGGCTGGGTCTGACGGTCAGCATTCTGGGCGCATTGCTGGTGGTGTTCCTGGCGAT
CCTCGCGTATTTCGCCCTGACCCACGAGCTGAACGCGCTATCCCGGGACAGCCTGGAAAAGAAGCTGGAACAGGTCGAGC
ACAGCCTGACGCTGTACTTCGACACCGGAGAGATCAGTGCCAAGCCGCACATTCTGCTCGATCAGGTCATGGGGCACGAC
AACCTGACCCTGACTATTTATGACCTGAACAACCTACGCACCCCGCTGCTCAAATCCGGTTCGGGCCTGACTGATCCACG
GGTCGAGTTGAAGGCGGTACGGGCGACGACCGATCAGTTGACCCACACCGACAGCGCAGATGCCGAAGGTGCGAAGTTTC
TCACCGTCTCCAAGCTGATTCGCCTCAAGGACGGCAGCAGCGTACCAGTCCTGCTGTCGATGGATAACGCCCACGATCAG
GCCTTGCTCAGTGCTTATTTGCGCTCGACGTTGATGGCTTTGCCGTTGCTGCTGGTATTCATCGGCCTGACCGCTTGGGG
CGCCGTGCAGCGAGGGTTGGCACCGTTGCGGGAATTTCGCAAAGTGGCAGCGCGAGTGACGACGCAGGATCTCGATCATC
GCCTGTCGGTGCTGAACATGCCCCAGGAACTCAGCGAACTGGCGCAGGGCATCAACGTGATGCTCGATCGCCTGGACAGC
GGCGTGCAGCAGTTGTCGCAATTCTCCGATGACCTGGCCCACGAGCTGCGCACGCCGATCAACAACCTGATGGGCAAGGC
TCAGGTGACGCTGTCGCGCGAGCGCTCGGTCGAGGAATACACCAACGTTCTGGTGTCCTGCACCGAAGAGCTGGAGCGGG
TCGCGCGGATTGTCTCGGACATGCTGTTCCTGGCCCAGGCCAGCCAGCCTGCGGCGCACGCCTCGTTCGAGCCGATTGCA
CTGGAAGAAGAGGCGTCCAGGGTGGCGGATCTGTTTTCCCTGACGGCACAGGACAAACAGATCGATCTGAAGATCATCGG
CAGCGCGCAGGTGTCGGGGGACCGGCTGATGATTCAACGAGCGATTTCCAATCTGCTGTCCAATGCCCTGCGCCACTGCC
CGTCCGGCAAGACCGTTTCGCTGTTCATCGAACGTGCGCCGAACGAGGTGTCGCTGCGGGTGAGCAATCCAGGCGCAGGC
ATCGAAGCGCAGCACCTGCCGTATCTGTTCGACCGTTTTTACCGGGTCGACAGCAGCCGCACCCGGTCACAGGGCAGTAC
CGGTCTGGGACTGGCCATCGTGCACTCGATCATGACCCTGCATCAGGGCTCTGCGCAGGTTCAAAGCCGACCGGGAGCGA
TGACAATATTCAGCCTGGTGTTTCCCGTGACGCACCCGGAACGGCACTGA

Upstream 100 bases:

>100_bases
AAGTCTCGATCCGCCGCTTGCGGGCGAAGATCGACGACCCGTTCGACAGCAAACTGATCCACACCTTGCGCGGCGTCGGC
TATGTGCTGGAGGCGCGGGA

Downstream 100 bases:

>100_bases
ACCTTCAAGCCGATTTGTGCGGTGTTTTCTCAAACCTCATGGGAAAAATCACCGCACAAGTGCTGCATACATCCCCGTAA
ATCCTTCAGCACGAATTTGC

Product: heavy metal sensor Signal transduction histidine kinase (STHK)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 469; Mature: 469

Protein sequence:

>469_residues
MKPASLSLRLGLTVSILGALLVVFLAILAYFALTHELNALSRDSLEKKLEQVEHSLTLYFDTGEISAKPHILLDQVMGHD
NLTLTIYDLNNLRTPLLKSGSGLTDPRVELKAVRATTDQLTHTDSADAEGAKFLTVSKLIRLKDGSSVPVLLSMDNAHDQ
ALLSAYLRSTLMALPLLLVFIGLTAWGAVQRGLAPLREFRKVAARVTTQDLDHRLSVLNMPQELSELAQGINVMLDRLDS
GVQQLSQFSDDLAHELRTPINNLMGKAQVTLSRERSVEEYTNVLVSCTEELERVARIVSDMLFLAQASQPAAHASFEPIA
LEEEASRVADLFSLTAQDKQIDLKIIGSAQVSGDRLMIQRAISNLLSNALRHCPSGKTVSLFIERAPNEVSLRVSNPGAG
IEAQHLPYLFDRFYRVDSSRTRSQGSTGLGLAIVHSIMTLHQGSAQVQSRPGAMTIFSLVFPVTHPERH

Sequences:

>Translated_469_residues
MKPASLSLRLGLTVSILGALLVVFLAILAYFALTHELNALSRDSLEKKLEQVEHSLTLYFDTGEISAKPHILLDQVMGHD
NLTLTIYDLNNLRTPLLKSGSGLTDPRVELKAVRATTDQLTHTDSADAEGAKFLTVSKLIRLKDGSSVPVLLSMDNAHDQ
ALLSAYLRSTLMALPLLLVFIGLTAWGAVQRGLAPLREFRKVAARVTTQDLDHRLSVLNMPQELSELAQGINVMLDRLDS
GVQQLSQFSDDLAHELRTPINNLMGKAQVTLSRERSVEEYTNVLVSCTEELERVARIVSDMLFLAQASQPAAHASFEPIA
LEEEASRVADLFSLTAQDKQIDLKIIGSAQVSGDRLMIQRAISNLLSNALRHCPSGKTVSLFIERAPNEVSLRVSNPGAG
IEAQHLPYLFDRFYRVDSSRTRSQGSTGLGLAIVHSIMTLHQGSAQVQSRPGAMTIFSLVFPVTHPERH
>Mature_469_residues
MKPASLSLRLGLTVSILGALLVVFLAILAYFALTHELNALSRDSLEKKLEQVEHSLTLYFDTGEISAKPHILLDQVMGHD
NLTLTIYDLNNLRTPLLKSGSGLTDPRVELKAVRATTDQLTHTDSADAEGAKFLTVSKLIRLKDGSSVPVLLSMDNAHDQ
ALLSAYLRSTLMALPLLLVFIGLTAWGAVQRGLAPLREFRKVAARVTTQDLDHRLSVLNMPQELSELAQGINVMLDRLDS
GVQQLSQFSDDLAHELRTPINNLMGKAQVTLSRERSVEEYTNVLVSCTEELERVARIVSDMLFLAQASQPAAHASFEPIA
LEEEASRVADLFSLTAQDKQIDLKIIGSAQVSGDRLMIQRAISNLLSNALRHCPSGKTVSLFIERAPNEVSLRVSNPGAG
IEAQHLPYLFDRFYRVDSSRTRSQGSTGLGLAIVHSIMTLHQGSAQVQSRPGAMTIFSLVFPVTHPERH

Specific function: Member of the two-component regulatory system irlR/irlS. May be involved in invasion of eukaryotic cells and heavy-metal resistance. Probably activates irlR by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 histidine kinase domain [H]

Homologues:

Organism=Escherichia coli, GI1786783, Length=318, Percent_Identity=41.8238993710692, Blast_Score=228, Evalue=5e-61,
Organism=Escherichia coli, GI1788279, Length=310, Percent_Identity=32.258064516129, Blast_Score=163, Evalue=3e-41,
Organism=Escherichia coli, GI1786600, Length=224, Percent_Identity=31.6964285714286, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1787894, Length=245, Percent_Identity=29.7959183673469, Blast_Score=100, Evalue=4e-22,
Organism=Escherichia coli, GI1788393, Length=254, Percent_Identity=27.9527559055118, Blast_Score=96, Evalue=6e-21,
Organism=Escherichia coli, GI1790346, Length=261, Percent_Identity=27.5862068965517, Blast_Score=92, Evalue=7e-20,
Organism=Escherichia coli, GI145693157, Length=254, Percent_Identity=27.1653543307087, Blast_Score=88, Evalue=1e-18,
Organism=Escherichia coli, GI1789403, Length=285, Percent_Identity=27.3684210526316, Blast_Score=83, Evalue=4e-17,
Organism=Escherichia coli, GI1790436, Length=223, Percent_Identity=26.457399103139, Blast_Score=82, Evalue=6e-17,
Organism=Escherichia coli, GI1789149, Length=336, Percent_Identity=23.8095238095238, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1790551, Length=304, Percent_Identity=27.6315789473684, Blast_Score=79, Evalue=9e-16,
Organism=Escherichia coli, GI1790861, Length=239, Percent_Identity=28.0334728033473, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1789808, Length=282, Percent_Identity=27.3049645390071, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1788549, Length=226, Percent_Identity=23.0088495575221, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI1788713, Length=357, Percent_Identity=23.8095238095238, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI48994928, Length=229, Percent_Identity=23.1441048034935, Blast_Score=64, Evalue=3e-11,
Organism=Escherichia coli, GI87081816, Length=231, Percent_Identity=28.5714285714286, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR004358
- InterPro:   IPR006290
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 51449; Mature: 51449

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: PS50885 HAMP ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPASLSLRLGLTVSILGALLVVFLAILAYFALTHELNALSRDSLEKKLEQVEHSLTLYF
CCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEE
DTGEISAKPHILLDQVMGHDNLTLTIYDLNNLRTPLLKSGSGLTDPRVELKAVRATTDQL
ECCCCCCCCHHHHHHHCCCCCCEEEEEECCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHH
THTDSADAEGAKFLTVSKLIRLKDGSSVPVLLSMDNAHDQALLSAYLRSTLMALPLLLVF
CCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
IGLTAWGAVQRGLAPLREFRKVAARVTTQDLDHRLSVLNMPQELSELAQGINVMLDRLDS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
GVQQLSQFSDDLAHELRTPINNLMGKAQVTLSRERSVEEYTNVLVSCTEELERVARIVSD
HHHHHHHHHHHHHHHHHHHHHHHCCCHHEEHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
MLFLAQASQPAAHASFEPIALEEEASRVADLFSLTAQDKQIDLKIIGSAQVSGDRLMIQR
HHHHHHCCCCCCCCCCCCEEEHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHH
AISNLLSNALRHCPSGKTVSLFIERAPNEVSLRVSNPGAGIEAQHLPYLFDRFYRVDSSR
HHHHHHHHHHHHCCCCCEEEEEEECCCCEEEEEECCCCCCCCCCCCHHHHHHHHHCCCCC
TRSQGSTGLGLAIVHSIMTLHQGSAQVQSRPGAMTIFSLVFPVTHPERH
CCCCCCCCHHHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKPASLSLRLGLTVSILGALLVVFLAILAYFALTHELNALSRDSLEKKLEQVEHSLTLYF
CCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEE
DTGEISAKPHILLDQVMGHDNLTLTIYDLNNLRTPLLKSGSGLTDPRVELKAVRATTDQL
ECCCCCCCCHHHHHHHCCCCCCEEEEEECCCCCCHHHCCCCCCCCCCHHHHHHHHHHHHH
THTDSADAEGAKFLTVSKLIRLKDGSSVPVLLSMDNAHDQALLSAYLRSTLMALPLLLVF
CCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHH
IGLTAWGAVQRGLAPLREFRKVAARVTTQDLDHRLSVLNMPQELSELAQGINVMLDRLDS
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHH
GVQQLSQFSDDLAHELRTPINNLMGKAQVTLSRERSVEEYTNVLVSCTEELERVARIVSD
HHHHHHHHHHHHHHHHHHHHHHHCCCHHEEHHHHCCHHHHHHHHHHHHHHHHHHHHHHHH
MLFLAQASQPAAHASFEPIALEEEASRVADLFSLTAQDKQIDLKIIGSAQVSGDRLMIQR
HHHHHHCCCCCCCCCCCCEEEHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHH
AISNLLSNALRHCPSGKTVSLFIERAPNEVSLRVSNPGAGIEAQHLPYLFDRFYRVDSSR
HHHHHHHHHHHHCCCCCEEEEEEECCCCEEEEEECCCCCCCCCCCCHHHHHHHHHCCCCC
TRSQGSTGLGLAIVHSIMTLHQGSAQVQSRPGAMTIFSLVFPVTHPERH
CCCCCCCCHHHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9393784 [H]