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The map label for this gene is nuoF [H]

Identifier: 77459829

GI number: 77459829

Start: 4076417

End: 4077772

Strand: Direct

Name: nuoF [H]

Synonym: Pfl01_3607

Alternate gene names: 77459829

Gene position: 4076417-4077772 (Clockwise)

Preceding gene: 77459828

Following gene: 77459830

Centisome position: 63.31

GC content: 63.57

Gene sequence:

>1356_bases
ATGACCCTGACTTCTTTCGGTCCGGCCAACCGCATCCAGCGTTCGGCCGAGACTCACCCGCTGACCTGGCGCCTGCGTGA
CGACGGCGAAGCCGTGTGGCTCGACGAGTACCAGGCCAAGAACGGTTACGCCGCTGCGCGCAAAGCCTTCGCCGACATGG
ATCAGGACGCCATCGTCCAGACCGTGAAAGACGCCGGCCTCAAAGGTCGCGGCGGTGCAGGCTTCCCCACAGGCGTGAAG
TGGGGCCTGATGCCAAAGGACGAATCCATCAACATCCGCTACCTGCTGTGCAACGCGGATGAAATGGAGCCGAACACCTG
GAAAGACCGCATGCTGATGGAGCAACTGCCCCATCTGCTGATCGAAGGCATGCTGATCAGCGCCCGCGCGCTGAAAACCT
ACCGTGGCTACATCTTCCTGCGTGGCGAATACACCACCGCCGCCAAGCACCTGAACCGTGCCGTGGAAGAAGCCAAGGCA
GCCGGCCTGCTGGGCAAGAACATCCTGGGCAGCGGCTTCGATTTCGAGCTGTTCGTCCACACCGGCGCCGGGCGTTACAT
CTGCGGCGAAGAAACCGCACTGATCAACTCCCTCGAAGGCCGCCGCGCCAACCCGCGCTCCAAACCGCCCTTCCCTGCCG
CCGTCGGCGTGTGGGGCAAGCCGACCTGCGTGAACAACGTTGAAACCCTGTGCAACGTGCCGGCGATCATTGCCGACGGC
GTGGACTGGTACAAATCGTTGGCCCGCGACGGCAGCGAAGACATGGGCACCAAGCTCATGGGCTTCTCCGGCAAGGTCAA
GAACCCCGGCCTGTGGGAACTGCCATTCGGCGTGACCGGCCGCGAGCTGTTCGAAGACTACGCCGGCGGCATGCGCGACG
GCTTCAAGCTGAAAGCCTGGCAGCCAGGTGGCGCCGGTACCGGTTTCCTGTTGCCGGAACACCTCGACGCACAAATGTAC
GCTGGCGGCATCGCCAAGGTTGGCACCCGTATGGGTACCGGCCTGGCCATGGCGGTGGACGACAGCGTCAACATGGTGTC
CCTGCTGCGCAACATGGAGCAGTTCTTCGCTCGCGAATCCTGTGGTTTCTGCACCCCTTGCCGTGATGGTTTGCCATGGA
GCGTCAAGCTGCTGATGGCCATCGAACAAGGCCGCGGCCAGCCAGGCGACATCGAGACCCTGCTGGGTCTGGTCAACTTC
CTCGGCCCGGGCAAGACCTTCTGTGCTCACGCACCGGGTGCCGTGGAGCCGTTGGGCAGTGCCATCAAATACTTCCGTCC
AGAGTTCGAAGCCGGTATCGCGCCTGCAAGCGCCGCCGTCCCGCCTCTGGCGAAGCCGATCGTAGTCGGCGCGTAA

Upstream 100 bases:

>100_bases
GCCTCGGCAACTGCGACAAGGCACCGGCGCTGATGATCGATGACGACACCTTTGGCGATGTCCAGCCTGCCGGCGTCGCC
AAACTGCTCGAGGGCTACGT

Downstream 100 bases:

>100_bases
ACGCTTAAAAAGGCGAAGGGTCCGTGCCCTTCGCCTTTCGTCCGATGACGCCTTTCGGGGCTGACTGGATTCGGACGGAT
AACAAGATTCCATTAGCCAC

Product: NADH dehydrogenase I subunit F

Products: NA

Alternate protein names: NADH dehydrogenase I subunit F; NDH-1 subunit F [H]

Number of amino acids: Translated: 451; Mature: 450

Protein sequence:

>451_residues
MTLTSFGPANRIQRSAETHPLTWRLRDDGEAVWLDEYQAKNGYAAARKAFADMDQDAIVQTVKDAGLKGRGGAGFPTGVK
WGLMPKDESINIRYLLCNADEMEPNTWKDRMLMEQLPHLLIEGMLISARALKTYRGYIFLRGEYTTAAKHLNRAVEEAKA
AGLLGKNILGSGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPAAVGVWGKPTCVNNVETLCNVPAIIADG
VDWYKSLARDGSEDMGTKLMGFSGKVKNPGLWELPFGVTGRELFEDYAGGMRDGFKLKAWQPGGAGTGFLLPEHLDAQMY
AGGIAKVGTRMGTGLAMAVDDSVNMVSLLRNMEQFFARESCGFCTPCRDGLPWSVKLLMAIEQGRGQPGDIETLLGLVNF
LGPGKTFCAHAPGAVEPLGSAIKYFRPEFEAGIAPASAAVPPLAKPIVVGA

Sequences:

>Translated_451_residues
MTLTSFGPANRIQRSAETHPLTWRLRDDGEAVWLDEYQAKNGYAAARKAFADMDQDAIVQTVKDAGLKGRGGAGFPTGVK
WGLMPKDESINIRYLLCNADEMEPNTWKDRMLMEQLPHLLIEGMLISARALKTYRGYIFLRGEYTTAAKHLNRAVEEAKA
AGLLGKNILGSGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPAAVGVWGKPTCVNNVETLCNVPAIIADG
VDWYKSLARDGSEDMGTKLMGFSGKVKNPGLWELPFGVTGRELFEDYAGGMRDGFKLKAWQPGGAGTGFLLPEHLDAQMY
AGGIAKVGTRMGTGLAMAVDDSVNMVSLLRNMEQFFARESCGFCTPCRDGLPWSVKLLMAIEQGRGQPGDIETLLGLVNF
LGPGKTFCAHAPGAVEPLGSAIKYFRPEFEAGIAPASAAVPPLAKPIVVGA
>Mature_450_residues
TLTSFGPANRIQRSAETHPLTWRLRDDGEAVWLDEYQAKNGYAAARKAFADMDQDAIVQTVKDAGLKGRGGAGFPTGVKW
GLMPKDESINIRYLLCNADEMEPNTWKDRMLMEQLPHLLIEGMLISARALKTYRGYIFLRGEYTTAAKHLNRAVEEAKAA
GLLGKNILGSGFDFELFVHTGAGRYICGEETALINSLEGRRANPRSKPPFPAAVGVWGKPTCVNNVETLCNVPAIIADGV
DWYKSLARDGSEDMGTKLMGFSGKVKNPGLWELPFGVTGRELFEDYAGGMRDGFKLKAWQPGGAGTGFLLPEHLDAQMYA
GGIAKVGTRMGTGLAMAVDDSVNMVSLLRNMEQFFARESCGFCTPCRDGLPWSVKLLMAIEQGRGQPGDIETLLGLVNFL
GPGKTFCAHAPGAVEPLGSAIKYFRPEFEAGIAPASAAVPPLAKPIVVGA

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1894

COG function: function code C; NADH:ubiquinone oxidoreductase, NADH-binding (51 kD) subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 51 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI260656005, Length=378, Percent_Identity=43.3862433862434, Blast_Score=300, Evalue=2e-81,
Organism=Homo sapiens, GI20149568, Length=378, Percent_Identity=43.3862433862434, Blast_Score=300, Evalue=2e-81,
Organism=Escherichia coli, GI1788620, Length=422, Percent_Identity=77.4881516587678, Blast_Score=686, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17531727, Length=382, Percent_Identity=42.1465968586387, Blast_Score=293, Evalue=1e-79,
Organism=Drosophila melanogaster, GI45550452, Length=383, Percent_Identity=43.6031331592689, Blast_Score=307, Evalue=8e-84,
Organism=Drosophila melanogaster, GI19920794, Length=378, Percent_Identity=43.3862433862434, Blast_Score=307, Evalue=1e-83,
Organism=Drosophila melanogaster, GI24653819, Length=376, Percent_Identity=33.7765957446808, Blast_Score=216, Evalue=2e-56,
Organism=Drosophila melanogaster, GI24653817, Length=354, Percent_Identity=32.2033898305085, Blast_Score=191, Evalue=9e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001949
- InterPro:   IPR019575
- InterPro:   IPR011537
- InterPro:   IPR011538
- InterPro:   IPR019554 [H]

Pfam domain/function: PF01512 Complex1_51K; PF10589 NADH_4Fe-4S; PF10531 SLBB [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 48742; Mature: 48610

Theoretical pI: Translated: 7.07; Mature: 7.07

Prosite motif: PS00644 COMPLEX1_51K_1 ; PS00645 COMPLEX1_51K_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLTSFGPANRIQRSAETHPLTWRLRDDGEAVWLDEYQAKNGYAAARKAFADMDQDAIVQ
CCCCCCCCHHHHHHCCCCCCEEEEECCCCCEEEEECCCCCCCHHHHHHHHHCCCHHHHHH
TVKDAGLKGRGGAGFPTGVKWGLMPKDESINIRYLLCNADEMEPNTWKDRMLMEQLPHLL
HHHHCCCCCCCCCCCCCCCEECCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHH
IEGMLISARALKTYRGYIFLRGEYTTAAKHLNRAVEEAKAAGLLGKNILGSGFDFELFVH
HHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
TGAGRYICGEETALINSLEGRRANPRSKPPFPAAVGVWGKPTCVNNVETLCNVPAIIADG
CCCCCEEECCHHHHHHCCCCCCCCCCCCCCCCCEECCCCCCCHHHHHHHHHCCCHHHHCC
VDWYKSLARDGSEDMGTKLMGFSGKVKNPGLWELPFGVTGRELFEDYAGGMRDGFKLKAW
HHHHHHHHCCCCHHHCCEEECCCCCCCCCCEEECCCCCCHHHHHHHHCCCCCCCCEEEEE
QPGGAGTGFLLPEHLDAQMYAGGIAKVGTRMGTGLAMAVDDSVNMVSLLRNMEQFFARES
CCCCCCCCCCCCCCCCHHHHHCHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHC
CGFCTPCRDGLPWSVKLLMAIEQGRGQPGDIETLLGLVNFLGPGKTFCAHAPGAVEPLGS
CCCCCCCCCCCCHHHHHEEHHHHCCCCCCHHHHHHHHHHHCCCCCCEECCCCCCHHHHHH
AIKYFRPEFEAGIAPASAAVPPLAKPIVVGA
HHHHHCCHHHCCCCCCCCCCCCCCCCEEECC
>Mature Secondary Structure 
TLTSFGPANRIQRSAETHPLTWRLRDDGEAVWLDEYQAKNGYAAARKAFADMDQDAIVQ
CCCCCCCHHHHHHCCCCCCEEEEECCCCCEEEEECCCCCCCHHHHHHHHHCCCHHHHHH
TVKDAGLKGRGGAGFPTGVKWGLMPKDESINIRYLLCNADEMEPNTWKDRMLMEQLPHLL
HHHHCCCCCCCCCCCCCCCEECCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHH
IEGMLISARALKTYRGYIFLRGEYTTAAKHLNRAVEEAKAAGLLGKNILGSGFDFELFVH
HHHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEE
TGAGRYICGEETALINSLEGRRANPRSKPPFPAAVGVWGKPTCVNNVETLCNVPAIIADG
CCCCCEEECCHHHHHHCCCCCCCCCCCCCCCCCEECCCCCCCHHHHHHHHHCCCHHHHCC
VDWYKSLARDGSEDMGTKLMGFSGKVKNPGLWELPFGVTGRELFEDYAGGMRDGFKLKAW
HHHHHHHHCCCCHHHCCEEECCCCCCCCCCEEECCCCCCHHHHHHHHCCCCCCCCEEEEE
QPGGAGTGFLLPEHLDAQMYAGGIAKVGTRMGTGLAMAVDDSVNMVSLLRNMEQFFARES
CCCCCCCCCCCCCCCCHHHHHCHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHHHHHHHC
CGFCTPCRDGLPWSVKLLMAIEQGRGQPGDIETLLGLVNFLGPGKTFCAHAPGAVEPLGS
CCCCCCCCCCCCHHHHHEEHHHHCCCCCCHHHHHHHHHHHCCCCCCEECCCCCCHHHHHH
AIKYFRPEFEAGIAPASAAVPPLAKPIVVGA
HHHHHCCHHHCCCCCCCCCCCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]