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The map label for this gene is purB [H]

Identifier: 77459820

GI number: 77459820

Start: 4066638

End: 4068053

Strand: Direct

Name: purB [H]

Synonym: Pfl01_3598

Alternate gene names: 77459820

Gene position: 4066638-4068053 (Clockwise)

Preceding gene: 77459819

Following gene: 77459821

Centisome position: 63.16

GC content: 61.37

Gene sequence:

>1416_bases
ATGATACGCGCCCCATTTCGTTGCCCGACTGTCCGAGAACACCCCATGCAGCTCTCTTCGCTCACTGCGGTTTCCCCTGT
TGACGGCCGCTACGCCGGCAAAACCCAGGCCCTGCGCCCAATTTTCAGCGAGTACGGTCTGATCCGTGCCCGCGTTCTGG
TTGAAGTGCGCTGGCTCCAGCGCCTGGCCGCTCACGCCGGCATCCCGGAAGTGCCAGCCTTCTCCGCCGAAGCCAACGCC
GTTCTGAATGAACTGGCCGAGAACTTCTCGCTAGAGCACGCCGAGCGCGTGAAAGAGATCGAGCGAACCACCAACCACGA
CGTAAAAGCGATCGAATACCTGCTCAAAGAGCAGGCAGCCAAGCTGCCGGAGCTGGCCAAGGTCAGCGAATTCATCCACT
TCGCCTGCACCAGCGAGGACATCAACAACCTGTCCCACGCCCTGATGCTGCGCGAAGGCCGTGACGACGTGATGCTGCCG
CTGATGCGCCAGACCGCCAACGCCATCCGCGAACTGGCCATCCGCTTCGCAGACGTACCGATGCTGTCGCGCACCCACGG
TCAGCCGGCTTCGCCGACCACCCTGGGTAAAGAGCTGGCGAACGTGGTTTACCGTCTCGAGCGTCAGATCGCTCAGGTCG
CTGCCGTTCCGCTGCTGGGCAAGATCAACGGCGCTGTCGGCAACTACAACGCTCACCTGTCCGCCTACCCGCAGATCGAC
TGGGAAGCCAACGCCCGCGCCTTCATCGAAGACGAGCTGGGTCTGGGCTTCAACCCGTACACCACGCAGATCGAGCCGCA
CGACTACATCGCCGAGCTGTTCGACGCCATTGCGCGCTTCAACACCATCCTGATCGACTTTGACCGTGACATCTGGGGCT
ACATCTCCCTGGGTTACTTCAAGCAGCGCACCATCGCCGGCGAAATCGGTTCGTCGACCATGCCGCACAAGGTCAACCCG
ATCGACTTCGAAAACTCCGAAGGCAACCTGGGCATCGCCAACGCGCTGTTCCAGCACCTGGCGAGCAAACTGCCGATCTC
CCGCTGGCAGCGCGATCTGACTGACTCCACCGTTCTGCGCAACCTCGGTGTGGGTTTCGCCCACAGTGTGATCGCGTACG
AAGCGAGCCTCAAAGGCATCAGCAAGCTCGAACTGAACGCTGACAAGATCGCTGCCGATCTGGACGCTTGCTGGGAAGTC
CTGGCCGAGCCGATCCAGACCGTGATGCGCCGCTACAACATCGAAAACCCGTACGAGAAGCTGAAAGAACTGACGCGCGG
CAAGGGCATCAGCCCTGAAGCGCTGCAGACTTTCATCGATGGCCTGGACATGCCGGCCGCTGCGAAAGCCGAGCTCAAGC
AACTGACCCCGGCCAACTACATCGGCAACGCTGTAGCGCAAGCCAAACGCATCTGA

Upstream 100 bases:

>100_bases
TTGATGCGCAGCGAGTAAATCACCGCGCAACAAAAGCTTTGTAGTCTGTAACGCGTAATACGCCGGTCAGTTGGCGACGG
ACCGGCGGATTTTTTCATGT

Downstream 100 bases:

>100_bases
TCGACTGCTTGACCCGTTTGAGACGCCCGGCCGCGCCGGGCGTTTTTATTCCCGTCTGAAAAATGCTTTTTTTCAATAGG
TTACATATGAATCCCGATAT

Product: adenylosuccinate lyase

Products: NA

Alternate protein names: ASL; Adenylosuccinase; ASase [H]

Number of amino acids: Translated: 471; Mature: 471

Protein sequence:

>471_residues
MIRAPFRCPTVREHPMQLSSLTAVSPVDGRYAGKTQALRPIFSEYGLIRARVLVEVRWLQRLAAHAGIPEVPAFSAEANA
VLNELAENFSLEHAERVKEIERTTNHDVKAIEYLLKEQAAKLPELAKVSEFIHFACTSEDINNLSHALMLREGRDDVMLP
LMRQTANAIRELAIRFADVPMLSRTHGQPASPTTLGKELANVVYRLERQIAQVAAVPLLGKINGAVGNYNAHLSAYPQID
WEANARAFIEDELGLGFNPYTTQIEPHDYIAELFDAIARFNTILIDFDRDIWGYISLGYFKQRTIAGEIGSSTMPHKVNP
IDFENSEGNLGIANALFQHLASKLPISRWQRDLTDSTVLRNLGVGFAHSVIAYEASLKGISKLELNADKIAADLDACWEV
LAEPIQTVMRRYNIENPYEKLKELTRGKGISPEALQTFIDGLDMPAAAKAELKQLTPANYIGNAVAQAKRI

Sequences:

>Translated_471_residues
MIRAPFRCPTVREHPMQLSSLTAVSPVDGRYAGKTQALRPIFSEYGLIRARVLVEVRWLQRLAAHAGIPEVPAFSAEANA
VLNELAENFSLEHAERVKEIERTTNHDVKAIEYLLKEQAAKLPELAKVSEFIHFACTSEDINNLSHALMLREGRDDVMLP
LMRQTANAIRELAIRFADVPMLSRTHGQPASPTTLGKELANVVYRLERQIAQVAAVPLLGKINGAVGNYNAHLSAYPQID
WEANARAFIEDELGLGFNPYTTQIEPHDYIAELFDAIARFNTILIDFDRDIWGYISLGYFKQRTIAGEIGSSTMPHKVNP
IDFENSEGNLGIANALFQHLASKLPISRWQRDLTDSTVLRNLGVGFAHSVIAYEASLKGISKLELNADKIAADLDACWEV
LAEPIQTVMRRYNIENPYEKLKELTRGKGISPEALQTFIDGLDMPAAAKAELKQLTPANYIGNAVAQAKRI
>Mature_471_residues
MIRAPFRCPTVREHPMQLSSLTAVSPVDGRYAGKTQALRPIFSEYGLIRARVLVEVRWLQRLAAHAGIPEVPAFSAEANA
VLNELAENFSLEHAERVKEIERTTNHDVKAIEYLLKEQAAKLPELAKVSEFIHFACTSEDINNLSHALMLREGRDDVMLP
LMRQTANAIRELAIRFADVPMLSRTHGQPASPTTLGKELANVVYRLERQIAQVAAVPLLGKINGAVGNYNAHLSAYPQID
WEANARAFIEDELGLGFNPYTTQIEPHDYIAELFDAIARFNTILIDFDRDIWGYISLGYFKQRTIAGEIGSSTMPHKVNP
IDFENSEGNLGIANALFQHLASKLPISRWQRDLTDSTVLRNLGVGFAHSVIAYEASLKGISKLELNADKIAADLDACWEV
LAEPIQTVMRRYNIENPYEKLKELTRGKGISPEALQTFIDGLDMPAAAKAELKQLTPANYIGNAVAQAKRI

Specific function: De novo purine biosynthesis; eighth step. [C]

COG id: COG0015

COG function: function code F; Adenylosuccinate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lyase 1 family. Adenylosuccinate lyase subfamily [H]

Homologues:

Organism=Escherichia coli, GI1787376, Length=450, Percent_Identity=69.7777777777778, Blast_Score=678, Evalue=0.0,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013539
- InterPro:   IPR000362
- InterPro:   IPR020557
- InterPro:   IPR008948
- InterPro:   IPR022761
- InterPro:   IPR004769 [H]

Pfam domain/function: PF08328 ASL_C; PF00206 Lyase_1 [H]

EC number: =4.3.2.2 [H]

Molecular weight: Translated: 52406; Mature: 52406

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: PS00163 FUMARATE_LYASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRAPFRCPTVREHPMQLSSLTAVSPVDGRYAGKTQALRPIFSEYGLIRARVLVEVRWLQ
CCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RLAAHAGIPEVPAFSAEANAVLNELAENFSLEHAERVKEIERTTNHDVKAIEYLLKEQAA
HHHHHCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
KLPELAKVSEFIHFACTSEDINNLSHALMLREGRDDVMLPLMRQTANAIRELAIRFADVP
CCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
MLSRTHGQPASPTTLGKELANVVYRLERQIAQVAAVPLLGKINGAVGNYNAHLSAYPQID
HHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCC
WEANARAFIEDELGLGFNPYTTQIEPHDYIAELFDAIARFNTILIDFDRDIWGYISLGYF
CCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCEEEEEECCCHHHHHHHHHH
KQRTIAGEIGSSTMPHKVNPIDFENSEGNLGIANALFQHLASKLPISRWQRDLTDSTVLR
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
NLGVGFAHSVIAYEASLKGISKLELNADKIAADLDACWEVLAEPIQTVMRRYNIENPYEK
HHCCHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
LKELTRGKGISPEALQTFIDGLDMPAAAKAELKQLTPANYIGNAVAQAKRI
HHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MIRAPFRCPTVREHPMQLSSLTAVSPVDGRYAGKTQALRPIFSEYGLIRARVLVEVRWLQ
CCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
RLAAHAGIPEVPAFSAEANAVLNELAENFSLEHAERVKEIERTTNHDVKAIEYLLKEQAA
HHHHHCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
KLPELAKVSEFIHFACTSEDINNLSHALMLREGRDDVMLPLMRQTANAIRELAIRFADVP
CCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCC
MLSRTHGQPASPTTLGKELANVVYRLERQIAQVAAVPLLGKINGAVGNYNAHLSAYPQID
HHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCC
WEANARAFIEDELGLGFNPYTTQIEPHDYIAELFDAIARFNTILIDFDRDIWGYISLGYF
CCCCCCEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCEEEEEECCCHHHHHHHHHH
KQRTIAGEIGSSTMPHKVNPIDFENSEGNLGIANALFQHLASKLPISRWQRDLTDSTVLR
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHH
NLGVGFAHSVIAYEASLKGISKLELNADKIAADLDACWEVLAEPIQTVMRRYNIENPYEK
HHCCHHHHHHHHHHHHHCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
LKELTRGKGISPEALQTFIDGLDMPAAAKAELKQLTPANYIGNAVAQAKRI
HHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]