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The map label for this gene is cspD [H]

Identifier: 77459814

GI number: 77459814

Start: 4059902

End: 4060183

Strand: Direct

Name: cspD [H]

Synonym: Pfl01_3592

Alternate gene names: 77459814

Gene position: 4059902-4060183 (Clockwise)

Preceding gene: 77459811

Following gene: 77459816

Centisome position: 63.06

GC content: 52.84

Gene sequence:

>282_bases
ATGGCTGTCGGCAAGGTGAAATGGTTCAACAATGCCAAGGGGTTCGGTTTCATTAATACCGACTCCCGCGAGGGGAAGGA
CGAGGACGGCAAGGAGATCGATTTCTTTGCCCACTATTCCGCCATTGAAATGGACGGATACAAAACCCTCAAAGCCGGGC
AAATCGTCAAATTCGAGATCGTGCAAGGGCCCAAAGGCCTGCATGCCACAAAAATTCAGAATGTCGAAGCCGCAAAAGAT
GCCGCCTCGGCCGCTACTCAGCACCAGTCAGTGACCAGCTGA

Upstream 100 bases:

>100_bases
GGGGAAAAAAGATCCGCGGAGTCAATCTTTTTAACGGATTCGACTGCGGATGAATTGGATGATACTCCAGCGATGGAGAC
TGTTGCAGAGGGATTTGAGC

Downstream 100 bases:

>100_bases
AGCAACCCGAACACCTGCCAGAAAACAAACCGCCCGGCTCGATCACTCGAGCCGGGCGGTTTCGTTGCAGCTGCCGTTCT
TACATGTGCTTGATCAGCGC

Product: cold-shock DNA-binding protein family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 93; Mature: 92

Protein sequence:

>93_residues
MAVGKVKWFNNAKGFGFINTDSREGKDEDGKEIDFFAHYSAIEMDGYKTLKAGQIVKFEIVQGPKGLHATKIQNVEAAKD
AASAATQHQSVTS

Sequences:

>Translated_93_residues
MAVGKVKWFNNAKGFGFINTDSREGKDEDGKEIDFFAHYSAIEMDGYKTLKAGQIVKFEIVQGPKGLHATKIQNVEAAKD
AASAATQHQSVTS
>Mature_92_residues
AVGKVKWFNNAKGFGFINTDSREGKDEDGKEIDFFAHYSAIEMDGYKTLKAGQIVKFEIVQGPKGLHATKIQNVEAAKDA
ASAATQHQSVTS

Specific function: Inhibits DNA Replication At Both Initiation And Elongation Steps, Most Probably By Binding To The Opened, Single- Stranded Regions At Replication Forks. Plays A Regulatory Role In Chromosomal Replication In Nutrient-Depleted Cells. [C]

COG id: COG1278

COG function: function code K; Cold shock proteins

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CSD (cold-shock) domain [H]

Homologues:

Organism=Escherichia coli, GI1787107, Length=76, Percent_Identity=61.8421052631579, Blast_Score=88, Evalue=1e-19,
Organism=Escherichia coli, GI1786841, Length=69, Percent_Identity=50.7246376811594, Blast_Score=66, Evalue=5e-13,
Organism=Escherichia coli, GI1788126, Length=69, Percent_Identity=49.2753623188406, Blast_Score=61, Evalue=2e-11,
Organism=Escherichia coli, GI1789979, Length=73, Percent_Identity=47.945205479452, Blast_Score=60, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019844
- InterPro:   IPR012156
- InterPro:   IPR011129
- InterPro:   IPR002059
- InterPro:   IPR012751
- InterPro:   IPR012340
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00313 CSD [H]

EC number: NA

Molecular weight: Translated: 10120; Mature: 9989

Theoretical pI: Translated: 7.79; Mature: 7.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVGKVKWFNNAKGFGFINTDSREGKDEDGKEIDFFAHYSAIEMDGYKTLKAGQIVKFEI
CCCCCEEECCCCCCCEEEECCCCCCCCCCCCEEEEEEECEEEEECCCEEECCCCEEEEEE
VQGPKGLHATKIQNVEAAKDAASAATQHQSVTS
ECCCCCCCCEEECCCHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
AVGKVKWFNNAKGFGFINTDSREGKDEDGKEIDFFAHYSAIEMDGYKTLKAGQIVKFEI
CCCCEEECCCCCCCEEEECCCCCCCCCCCCEEEEEEECEEEEECCCEEECCCCEEEEEE
VQGPKGLHATKIQNVEAAKDAASAATQHQSVTS
ECCCCCCCCEEECCCHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]