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The map label for this gene is ogt [H]

Identifier: 77459788

GI number: 77459788

Start: 4037009

End: 4037524

Strand: Direct

Name: ogt [H]

Synonym: Pfl01_3566

Alternate gene names: 77459788

Gene position: 4037009-4037524 (Clockwise)

Preceding gene: 77459784

Following gene: 77459793

Centisome position: 62.7

GC content: 60.85

Gene sequence:

>516_bases
ATGGCCTACACGTTCATCACCCTGCCCTCGCCCGTCGGCGAGTTGAAGCTGGTCGCGAACGGCTCACGTCTGGCCGCCAT
CCTCTGGGAAAACGACAAACCGAACCGAGTGCGCCTCGGGCCAATGAGCGAGGCACCGGACAACCCGGTATTGATGAAAA
CCGCGCGACAGCTGGAAGAATATTTTGCAGGAACGCGAAACGCGTTCGATCTGGAGCTGGACTTTGCCGGCACCGAGTTC
CAGAAAAAAGTCTGGGCCGCGCTACTGACCATTCCGTTTGGCGAAACCCGGACCTACAGCCAGATCGCCGAACAGATCGG
CCACCCGAGCGCGGTGCGTGCCGTTGGCGCGGCGAACGGGCGCAATCCGATTTCGATCATCGCGCCGTGCCATCGAGTCA
TCGGCGCCTCAGGAAAACTGACCGGGTTTGCCGGAGGCCTGGAAGCGAAGGAGCGCTTGCTGACTCTGGAGGGTGGCCAG
TGGTCGGACATCGGTAAAACCGGCGATCTGTTTTAA

Upstream 100 bases:

>100_bases
GCTTAGAGCATCAGCCAGTATGGTCGTGATTGCGGTTTGCGCCTCCCCGATCCGGCAGTATGCTGGGCGCAGTTTTCGCG
CCCCTCCCGGAGTTTTTCCC

Downstream 100 bases:

>100_bases
CCGCCAAACAGGTTATTCATCGCCGCATACTGCAGCAGCATGATGGTCTTGGCATCGCAGATTTCACCCCGCTGGAACGC
CGCCAGCGCGTCCTCGAAAC

Product: methylated-DNA-(protein)-cysteine S-methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 171; Mature: 170

Protein sequence:

>171_residues
MAYTFITLPSPVGELKLVANGSRLAAILWENDKPNRVRLGPMSEAPDNPVLMKTARQLEEYFAGTRNAFDLELDFAGTEF
QKKVWAALLTIPFGETRTYSQIAEQIGHPSAVRAVGAANGRNPISIIAPCHRVIGASGKLTGFAGGLEAKERLLTLEGGQ
WSDIGKTGDLF

Sequences:

>Translated_171_residues
MAYTFITLPSPVGELKLVANGSRLAAILWENDKPNRVRLGPMSEAPDNPVLMKTARQLEEYFAGTRNAFDLELDFAGTEF
QKKVWAALLTIPFGETRTYSQIAEQIGHPSAVRAVGAANGRNPISIIAPCHRVIGASGKLTGFAGGLEAKERLLTLEGGQ
WSDIGKTGDLF
>Mature_170_residues
AYTFITLPSPVGELKLVANGSRLAAILWENDKPNRVRLGPMSEAPDNPVLMKTARQLEEYFAGTRNAFDLELDFAGTEFQ
KKVWAALLTIPFGETRTYSQIAEQIGHPSAVRAVGAANGRNPISIIAPCHRVIGASGKLTGFAGGLEAKERLLTLEGGQW
SDIGKTGDLF

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=171, Percent_Identity=34.5029239766082, Blast_Score=76, Evalue=1e-14,
Organism=Escherichia coli, GI1787596, Length=161, Percent_Identity=44.7204968944099, Blast_Score=123, Evalue=8e-30,
Organism=Escherichia coli, GI1788542, Length=148, Percent_Identity=41.2162162162162, Blast_Score=97, Evalue=5e-22,
Organism=Caenorhabditis elegans, GI115533068, Length=83, Percent_Identity=53.0120481927711, Blast_Score=95, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI115533070, Length=83, Percent_Identity=53.0120481927711, Blast_Score=95, Evalue=2e-20,
Organism=Saccharomyces cerevisiae, GI6320001, Length=81, Percent_Identity=46.9135802469136, Blast_Score=89, Evalue=5e-19,
Organism=Drosophila melanogaster, GI17137554, Length=121, Percent_Identity=38.0165289256198, Blast_Score=66, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 18471; Mature: 18340

Theoretical pI: Translated: 7.52; Mature: 7.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYTFITLPSPVGELKLVANGSRLAAILWENDKPNRVRLGPMSEAPDNPVLMKTARQLEE
CEEEEEECCCCCCCEEEEECCCEEEEEEECCCCCCEEEECCCCCCCCCCHHHHHHHHHHH
YFAGTRNAFDLELDFAGTEFQKKVWAALLTIPFGETRTYSQIAEQIGHPSAVRAVGAANG
HHCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHEECCCCC
RNPISIIAPCHRVIGASGKLTGFAGGLEAKERLLTLEGGQWSDIGKTGDLF
CCCEEEEEHHHHHHCCCCCEEEECCCCCCCCEEEEECCCCCCCCCCCCCCC
>Mature Secondary Structure 
AYTFITLPSPVGELKLVANGSRLAAILWENDKPNRVRLGPMSEAPDNPVLMKTARQLEE
EEEEEECCCCCCCEEEEECCCEEEEEEECCCCCCEEEECCCCCCCCCCHHHHHHHHHHH
YFAGTRNAFDLELDFAGTEFQKKVWAALLTIPFGETRTYSQIAEQIGHPSAVRAVGAANG
HHCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHCCCHHHHEECCCCC
RNPISIIAPCHRVIGASGKLTGFAGGLEAKERLLTLEGGQWSDIGKTGDLF
CCCEEEEEHHHHHHCCCCCEEEECCCCCCCCEEEEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]