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The map label for this gene is rutR [H]

Identifier: 77459784

GI number: 77459784

Start: 4032758

End: 4033408

Strand: Direct

Name: rutR [H]

Synonym: Pfl01_3562

Alternate gene names: 77459784

Gene position: 4032758-4033408 (Clockwise)

Preceding gene: 77459781

Following gene: 77459788

Centisome position: 62.64

GC content: 64.21

Gene sequence:

>651_bases
ATGCCGTCCATGAGCAGCATCCGCGAACGCAACCAGCAACTGATCCTGGCCGCCGCCAGTGAAGAATTCGCCGCCAACGG
CTTCGACGCGACCCAGACCCGCGACATCGCCGCCCGCGCCGGTGTGCCCAAGGCCAACCTGTATTACTACTTCCAGAGCA
AGGAAAACCTCTACGGCAAAGTGCTGCTCGGCTTCGTCGAACCGTTGCTGGAAGCTTCGGCGGTGCTGCGCGAAAGCGAC
GATCCGCTGATTGGCCTGCGCGCCTACGTCGCCGCCCGCATCCGCATCGCCCGCGAGCATCCGGCGATCGCCAAGGTTTT
CAGTGGTGAGTTGCTGCTCGGCGGCCGTCAGTTGCCCGACGAATGCCGCGACCTGCTGCACGGTGAGGCCCGGCGCAATG
TCGAGTGTCTGCGCAGCTGGATCGACCGCGGGCTGCTGGCGCCGGTCGATCCAGAGCATTTGATGCTGTTTATCTGGTCG
GCGACGCGCACCTACACCAACATTGGCTGGCAGATGGGGCGCATCACCGGGCGGGAGGTGCCGCAGGATGAGGATTATGC
GGTGGCAGCGGAGACGATTACGCGGCTGGTGCTGAGCGGTGCGGTGTCCGAGCCGGTGAGTGATGTTCGCAGGTTGATGT
TTGCGACCTGA

Upstream 100 bases:

>100_bases
TGTCACTGAAATTGAAACCAGCATAGCGCTGAGGAGCGAGTTCGCTGGCCTAATCGCTATATAGTTTTGTATATTGCGAA
GCCGAAATGGCCAGACCTCC

Downstream 100 bases:

>100_bases
ACGTGAGAGTCAGGGCATCAGGTCCAGAGGCAGTGTCGCCTCTCCCGCCAAGCGTTCACGCCACGCCAACAGCACATCAG
GCACCCGCTTTTTCCATTTG

Product: TetR family transcriptional regulator

Products: NA

Alternate protein names: Rut operon repressor [H]

Number of amino acids: Translated: 216; Mature: 215

Protein sequence:

>216_residues
MPSMSSIRERNQQLILAAASEEFAANGFDATQTRDIAARAGVPKANLYYYFQSKENLYGKVLLGFVEPLLEASAVLRESD
DPLIGLRAYVAARIRIAREHPAIAKVFSGELLLGGRQLPDECRDLLHGEARRNVECLRSWIDRGLLAPVDPEHLMLFIWS
ATRTYTNIGWQMGRITGREVPQDEDYAVAAETITRLVLSGAVSEPVSDVRRLMFAT

Sequences:

>Translated_216_residues
MPSMSSIRERNQQLILAAASEEFAANGFDATQTRDIAARAGVPKANLYYYFQSKENLYGKVLLGFVEPLLEASAVLRESD
DPLIGLRAYVAARIRIAREHPAIAKVFSGELLLGGRQLPDECRDLLHGEARRNVECLRSWIDRGLLAPVDPEHLMLFIWS
ATRTYTNIGWQMGRITGREVPQDEDYAVAAETITRLVLSGAVSEPVSDVRRLMFAT
>Mature_215_residues
PSMSSIRERNQQLILAAASEEFAANGFDATQTRDIAARAGVPKANLYYYFQSKENLYGKVLLGFVEPLLEASAVLRESDD
PLIGLRAYVAARIRIAREHPAIAKVFSGELLLGGRQLPDECRDLLHGEARRNVECLRSWIDRGLLAPVDPEHLMLFIWSA
TRTYTNIGWQMGRITGREVPQDEDYAVAAETITRLVLSGAVSEPVSDVRRLMFAT

Specific function: Master transcription regulator which represses the degradation of pyrimidines (rutABCDEFG) and purines (gcl operon) for maintenance of metabolic balance between pyrimidines and purines. It also regulates the synthesis of pyrimidine nucleotides and arginin

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH tetR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787249, Length=190, Percent_Identity=32.1052631578947, Blast_Score=117, Evalue=4e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR015893
- InterPro:   IPR011075
- InterPro:   IPR019915
- InterPro:   IPR001647
- InterPro:   IPR013573 [H]

Pfam domain/function: PF08362 TetR_C_3; PF00440 TetR_N [H]

EC number: NA

Molecular weight: Translated: 24093; Mature: 23962

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: PS50977 HTH_TETR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSMSSIRERNQQLILAAASEEFAANGFDATQTRDIAARAGVPKANLYYYFQSKENLYGK
CCCHHHHHHCCCEEEEEECCHHHHCCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCHHHH
VLLGFVEPLLEASAVLRESDDPLIGLRAYVAARIRIAREHPAIAKVFSGELLLGGRQLPD
HHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHCCCHHHHHHCCCEEECCCCCCH
ECRDLLHGEARRNVECLRSWIDRGLLAPVDPEHLMLFIWSATRTYTNIGWQMGRITGREV
HHHHHHCCHHCCCHHHHHHHHHCCCCCCCCHHHEEEEEEECHHHHHHCCCCCCCCCCCCC
PQDEDYAVAAETITRLVLSGAVSEPVSDVRRLMFAT
CCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCC
>Mature Secondary Structure 
PSMSSIRERNQQLILAAASEEFAANGFDATQTRDIAARAGVPKANLYYYFQSKENLYGK
CCHHHHHHCCCEEEEEECCHHHHCCCCCCHHHHHHHHHCCCCCCEEEEEECCCCCHHHH
VLLGFVEPLLEASAVLRESDDPLIGLRAYVAARIRIAREHPAIAKVFSGELLLGGRQLPD
HHHHHHHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHCCCHHHHHHCCCEEECCCCCCH
ECRDLLHGEARRNVECLRSWIDRGLLAPVDPEHLMLFIWSATRTYTNIGWQMGRITGREV
HHHHHHCCHHCCCHHHHHHHHHCCCCCCCCHHHEEEEEEECHHHHHHCCCCCCCCCCCCC
PQDEDYAVAAETITRLVLSGAVSEPVSDVRRLMFAT
CCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]