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The map label for this gene is pleD [H]

Identifier: 77459772

GI number: 77459772

Start: 4022221

End: 4023900

Strand: Direct

Name: pleD [H]

Synonym: Pfl01_3550

Alternate gene names: 77459772

Gene position: 4022221-4023900 (Clockwise)

Preceding gene: 77459770

Following gene: 77459773

Centisome position: 62.47

GC content: 62.56

Gene sequence:

>1680_bases
ATGCTCAAAGCCAGTCTGCGCAGCCACCTGACCCTATGGTTTGCCGGTTTGTCTCTGCTGACGCTGTTGAGCGTGGGCTT
CTACGTCGGTGATATCGCCACCGAACAAATGAAACGCGCCAGCGGCAATGCGCTACTGAGTACCGCGCGGTCGGCGGCGG
CGCTGCTGGGCGAGCAGCTGCGCGAACGGCAACTGGAGGTCTATCTGCTCAGCCGGGCGCCGCATCTGGAACGCGGTGAT
CTGGACAATCCGGCGATTCTCAAATCGATGCAGCTGCGCACCGAGGCCCGCACCGAGTATGCGTGGATGGGGGTGACCGA
TGCTCAGGGCCGTGTGCATCAGGCAGTCAACGGTTTGCTGGTCGGTCAGGCCGTGCAACAACGCCCCTGGTTCCAGGCTG
GTCTGCGCGGTGAATACACCGGCGATCCGCACGAAGCCGTGTTGTTGGCGAAACTGTTGCCAGGCCTGCCCAACGGCGAG
CCACTGCGCTTCATCGACTTCGCCGCGCCTATCCGCAATGCCGATGGTCAGGTGATCGGTGTGCTCGGCGCCCATGCGCA
CTGGAGCTGGGTTACGCATATCGTCGAATCCGCAGCCATGTCGCACAAGGGAGCGGAGCCGGACATCGAAGCGCTGATCA
TCGATCACGACGGCAAGGTGCTGTACCCGGAAGCGCTGGTTGGCCAGCAGATGCCGGCCAAGGATCCGAACTCCCCGGGA
TGGGTCGTCGGCAACGGGTACCTGACCAGCGAGGTGACGGTACCGACTCCTACAGGCACCGCGCTGTCGTGGTCGATTGC
CGTGCGCCAACCACTGGACACTGCGCTGCAACCGGCGCGACTGCTGTTTTATAAATTGCTGCTGCTGGGGGTTTTCGCCG
CCGTGGCGTTCGGGCTCGTGGCGTATTACCTGGCGCTGTACCTGAGCCGGCCCATCGAGCAACTCGCGCGTTCTGCGCAA
AAAGTGCAGAACAACCAACCGGGGGCCCACTTCCCGCTCCGGCATCCCGTGCGGGAAATTGCCCAGCTCGGCCAGTCCAT
CGATGCCATGACCCAGTCGCTGCTCGCCAAGGAACGTGAGCTGCAGGAGACCAACGCATCGCTCGAAGCCACCGTCGCCC
AACGCACAGCGGCCCTGACCCTGGCCAACGCCGAGCTGCTGAACCTGGCCACCCACGATGGCTTGACCGGTGTCTACAAC
CGGCGCCGCTTCGACGAAAAACTCACCGAATACAGCCTGTTGTTCCGTCGTACCGGACGACCGTTTGCACTGCTTCTGAT
TGACGCCGATCACTTCAAGCGCATCAACGACACTCATGGTCATGCGGCCGGCGACGACGTACTGCGGCAATTGGCGCAAC
TGATCCAGATCAGTATCCGGAGCACGGATTTCGTCGCCCGTTACGGAGGCGAAGAATTCGCTGTGCTGCTGCCGGAAATC
GCCAAGCCCGATACTCCCGATATCGTCGCCGAGAAGATTCGGTCAGCGATTGCCGAGGCGGACTTTCCAGCGGTCGGCCC
GGTGACGGTCAGCATTGGTGTCAGCCTGGCCGACCCGGCCGACAACACCCCGACAACGCTGGTCAAACGCGCCGACCAGC
AGTTGTACAAGGCAAAAGCGGCAGGCCGCAATCAAGTGGCCTGCCAGGCTTCGCTCTTCGAGGTGAGCGAAAATGGCTGA

Upstream 100 bases:

>100_bases
TGGTATCAATCCCGGAAGGTTTCATTAAACGGCCTTGCTGGCAACCGATCCGCCATGTCGCATACCATGGCGCCACTCCA
ATAAAGACAGACATTTCTCC

Downstream 100 bases:

>100_bases
TGCGCACTCGATCCCGCCACGATTCATCCTCCACGAAACCGAACACTGGATACTCAATCACCACATGGCTTCGTCCCTGC
CCGGCTATCTGATGCTGGGA

Product: diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 559; Mature: 559

Protein sequence:

>559_residues
MLKASLRSHLTLWFAGLSLLTLLSVGFYVGDIATEQMKRASGNALLSTARSAAALLGEQLRERQLEVYLLSRAPHLERGD
LDNPAILKSMQLRTEARTEYAWMGVTDAQGRVHQAVNGLLVGQAVQQRPWFQAGLRGEYTGDPHEAVLLAKLLPGLPNGE
PLRFIDFAAPIRNADGQVIGVLGAHAHWSWVTHIVESAAMSHKGAEPDIEALIIDHDGKVLYPEALVGQQMPAKDPNSPG
WVVGNGYLTSEVTVPTPTGTALSWSIAVRQPLDTALQPARLLFYKLLLLGVFAAVAFGLVAYYLALYLSRPIEQLARSAQ
KVQNNQPGAHFPLRHPVREIAQLGQSIDAMTQSLLAKERELQETNASLEATVAQRTAALTLANAELLNLATHDGLTGVYN
RRRFDEKLTEYSLLFRRTGRPFALLLIDADHFKRINDTHGHAAGDDVLRQLAQLIQISIRSTDFVARYGGEEFAVLLPEI
AKPDTPDIVAEKIRSAIAEADFPAVGPVTVSIGVSLADPADNTPTTLVKRADQQLYKAKAAGRNQVACQASLFEVSENG

Sequences:

>Translated_559_residues
MLKASLRSHLTLWFAGLSLLTLLSVGFYVGDIATEQMKRASGNALLSTARSAAALLGEQLRERQLEVYLLSRAPHLERGD
LDNPAILKSMQLRTEARTEYAWMGVTDAQGRVHQAVNGLLVGQAVQQRPWFQAGLRGEYTGDPHEAVLLAKLLPGLPNGE
PLRFIDFAAPIRNADGQVIGVLGAHAHWSWVTHIVESAAMSHKGAEPDIEALIIDHDGKVLYPEALVGQQMPAKDPNSPG
WVVGNGYLTSEVTVPTPTGTALSWSIAVRQPLDTALQPARLLFYKLLLLGVFAAVAFGLVAYYLALYLSRPIEQLARSAQ
KVQNNQPGAHFPLRHPVREIAQLGQSIDAMTQSLLAKERELQETNASLEATVAQRTAALTLANAELLNLATHDGLTGVYN
RRRFDEKLTEYSLLFRRTGRPFALLLIDADHFKRINDTHGHAAGDDVLRQLAQLIQISIRSTDFVARYGGEEFAVLLPEI
AKPDTPDIVAEKIRSAIAEADFPAVGPVTVSIGVSLADPADNTPTTLVKRADQQLYKAKAAGRNQVACQASLFEVSENG
>Mature_559_residues
MLKASLRSHLTLWFAGLSLLTLLSVGFYVGDIATEQMKRASGNALLSTARSAAALLGEQLRERQLEVYLLSRAPHLERGD
LDNPAILKSMQLRTEARTEYAWMGVTDAQGRVHQAVNGLLVGQAVQQRPWFQAGLRGEYTGDPHEAVLLAKLLPGLPNGE
PLRFIDFAAPIRNADGQVIGVLGAHAHWSWVTHIVESAAMSHKGAEPDIEALIIDHDGKVLYPEALVGQQMPAKDPNSPG
WVVGNGYLTSEVTVPTPTGTALSWSIAVRQPLDTALQPARLLFYKLLLLGVFAAVAFGLVAYYLALYLSRPIEQLARSAQ
KVQNNQPGAHFPLRHPVREIAQLGQSIDAMTQSLLAKERELQETNASLEATVAQRTAALTLANAELLNLATHDGLTGVYN
RRRFDEKLTEYSLLFRRTGRPFALLLIDADHFKRINDTHGHAAGDDVLRQLAQLIQISIRSTDFVARYGGEEFAVLLPEI
AKPDTPDIVAEKIRSAIAEADFPAVGPVTVSIGVSLADPADNTPTTLVKRADQQLYKAKAAGRNQVACQASLFEVSENG

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG3706

COG function: function code T; Response regulator containing a CheY-like receiver domain and a GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1786584, Length=165, Percent_Identity=39.3939393939394, Blast_Score=115, Evalue=8e-27,
Organism=Escherichia coli, GI87081881, Length=162, Percent_Identity=40.7407407407407, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1787262, Length=165, Percent_Identity=38.7878787878788, Blast_Score=112, Evalue=6e-26,
Organism=Escherichia coli, GI145693134, Length=240, Percent_Identity=32.0833333333333, Blast_Score=108, Evalue=9e-25,
Organism=Escherichia coli, GI87082007, Length=162, Percent_Identity=40.7407407407407, Blast_Score=107, Evalue=3e-24,
Organism=Escherichia coli, GI1788381, Length=160, Percent_Identity=37.5, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1788085, Length=165, Percent_Identity=36.3636363636364, Blast_Score=96, Evalue=7e-21,
Organism=Escherichia coli, GI1787802, Length=165, Percent_Identity=38.1818181818182, Blast_Score=89, Evalue=9e-19,
Organism=Escherichia coli, GI1787816, Length=176, Percent_Identity=35.2272727272727, Blast_Score=79, Evalue=6e-16,
Organism=Escherichia coli, GI1787056, Length=168, Percent_Identity=34.5238095238095, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI87081974, Length=153, Percent_Identity=33.3333333333333, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI87081977, Length=174, Percent_Identity=31.0344827586207, Blast_Score=70, Evalue=3e-13,
Organism=Escherichia coli, GI1788956, Length=170, Percent_Identity=32.9411764705882, Blast_Score=68, Evalue=2e-12,
Organism=Escherichia coli, GI1787541, Length=171, Percent_Identity=28.0701754385965, Blast_Score=66, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 60842; Mature: 60842

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: PS50885 HAMP ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKASLRSHLTLWFAGLSLLTLLSVGFYVGDIATEQMKRASGNALLSTARSAAALLGEQL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
RERQLEVYLLSRAPHLERGDLDNPAILKSMQLRTEARTEYAWMGVTDAQGRVHQAVNGLL
HHHHHEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHH
VGQAVQQRPWFQAGLRGEYTGDPHEAVLLAKLLPGLPNGEPLRFIDFAAPIRNADGQVIG
HHHHHHHCCCHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCEEE
VLGAHAHWSWVTHIVESAAMSHKGAEPDIEALIIDHDGKVLYPEALVGQQMPAKDPNSPG
EEECCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCEECCHHHHCCCCCCCCCCCCC
WVVGNGYLTSEVTVPTPTGTALSWSIAVRQPLDTALQPARLLFYKLLLLGVFAAVAFGLV
EEEECCEEECEEEECCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AYYLALYLSRPIEQLARSAQKVQNNQPGAHFPLRHPVREIAQLGQSIDAMTQSLLAKERE
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQETNASLEATVAQRTAALTLANAELLNLATHDGLTGVYNRRRFDEKLTEYSLLFRRTGR
HHHHCCHHHHHHHHHHHHHEECCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
PFALLLIDADHFKRINDTHGHAAGDDVLRQLAQLIQISIRSTDFVARYGGEEFAVLLPEI
CEEEEEEECHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHCCCCEEEEECCHH
AKPDTPDIVAEKIRSAIAEADFPAVGPVTVSIGVSLADPADNTPTTLVKRADQQLYKAKA
CCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHH
AGRNQVACQASLFEVSENG
CCCCCEEHHHHHHHCCCCC
>Mature Secondary Structure
MLKASLRSHLTLWFAGLSLLTLLSVGFYVGDIATEQMKRASGNALLSTARSAAALLGEQL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
RERQLEVYLLSRAPHLERGDLDNPAILKSMQLRTEARTEYAWMGVTDAQGRVHQAVNGLL
HHHHHEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHH
VGQAVQQRPWFQAGLRGEYTGDPHEAVLLAKLLPGLPNGEPLRFIDFAAPIRNADGQVIG
HHHHHHHCCCHHHCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCEEE
VLGAHAHWSWVTHIVESAAMSHKGAEPDIEALIIDHDGKVLYPEALVGQQMPAKDPNSPG
EEECCHHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCEECCHHHHCCCCCCCCCCCCC
WVVGNGYLTSEVTVPTPTGTALSWSIAVRQPLDTALQPARLLFYKLLLLGVFAAVAFGLV
EEEECCEEECEEEECCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AYYLALYLSRPIEQLARSAQKVQNNQPGAHFPLRHPVREIAQLGQSIDAMTQSLLAKERE
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQETNASLEATVAQRTAALTLANAELLNLATHDGLTGVYNRRRFDEKLTEYSLLFRRTGR
HHHHCCHHHHHHHHHHHHHEECCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCC
PFALLLIDADHFKRINDTHGHAAGDDVLRQLAQLIQISIRSTDFVARYGGEEFAVLLPEI
CEEEEEEECHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHCCCCEEEEECCHH
AKPDTPDIVAEKIRSAIAEADFPAVGPVTVSIGVSLADPADNTPTTLVKRADQQLYKAKA
CCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHH
AGRNQVACQASLFEVSENG
CCCCCEEHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA