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The map label for this gene is topB [H]

Identifier: 77459586

GI number: 77459586

Start: 3840775

End: 3842718

Strand: Direct

Name: topB [H]

Synonym: Pfl01_3364

Alternate gene names: 77459586

Gene position: 3840775-3842718 (Clockwise)

Preceding gene: 77459584

Following gene: 77459587

Centisome position: 59.65

GC content: 64.92

Gene sequence:

>1944_bases
ATGCGGCTGTACCTCTGTGAAAAACCCTCCCAAGCCAAGGACATTGCGGCCGTACTCGGCGCAAAGCGTCGGGGCGACGG
CTGCTGGCTGGGAACGGACGTCACGGTGACCTGGTGCATCGGCCACCTGCTGGAAACCGCGCCGCCGGACGCCTACGACG
CCAAATACAAGCGCTGGGTGCTGGCCGACCTGCCGATCATCCCCGACAAGTGGAAAATGACTGTCAAGCCGCGTACCGCC
AGCCAGTACAAGGCGGTCAAGCGCCTGCTCGGCGAGGCCAGCGAACTGATCATCGCCACCGACGCCGACCGTGAAGGCGA
GATGATCGCCCGTGAGCTGGTCGAACACTGTCGCTATCGCGGGCCGATCCGCCGCTTGTGGCTGTCGGCGCTGGACGAAG
CCTCGATTCGCAAGGCGTTGGCGGCACTCAAACCGGGCGCCGAAACCTTCAGCCTGTATCACTCGGCACTGGGGCGCTCG
CGGGCGGACTGGCTGATCGGGATGAACATGAGTCGGCTGTTCACCCTGCTCGGCCGGCAATCGGGCTATCAAGGCGTGTT
GCCGGTGGGCCGGGTACAGACGCCGACGTTGCGACTGGTGGTGGATCGCGACCGCAGCATCGCTGACTTCGTGCCGGTCG
CGTACTGGGCCATCGACGTGCAACTGCTGCACGGAGACACGCCGTTCAACGCGCAATGGCGTGCACCGAACGATGCCTGT
GACGATCAGGATCGCTGCCTCAATCAAGCCCTCGCCCAGCAAGCGGCCGCCGCAATCAGTAGCGCTGCCAGCGCCCGGGT
GGTCAAGCTGCGCACCGAACGCATGCGCGAAGTGGCGCCCCTGCCCTTCGATCTGGGCACCTTGCAGGAAGTCTGTTCGA
AGAAGCTCGGCCTCGGTGCCCAGGAAACCCTCGACATCGCCCAGGCCCTCTACGAAACCTACAAAGTCATCACCTACCCG
CGCAGCGACTGCGGCTACCTGCCCCTGAGCCAGCACAACGAGGCGCCGGGGATTCTCGCAGCGCTGCGTCAGGCCGACCC
GAGCCTGGAAGCGCTGCGCGAGCATCTGGAACCACAGCGTCGCTCCCGCGCCTGGAACGACGCCAAGGTCAGCGCTCACC
ACGGCATCATCCCGACCGCTGCCGCGAAAAACCTGGAGCGCCTGACCGGCAAGCACCGCGCGGTCTACACGCTGATTCGC
GCGCGGTATCTGGCGCAGTTCCTGCCCAATCACGAATACGACCGCACCCAGGCCGATTTCGATTGCGCCGGGGAAGCCTT
GCGCGCTGTCGGCAAACAGATCATCGAGCCGGGCTGGAAACGCGCGTTGCCCGAAGCGCTGGCCCCGGCCAAGGGCCGTG
AAGCCCCCGCGCCACAAACACTACCGACACTGACCCAGGGTGCCGAATGCGCCGTGGCAGACGTGAAGCTCAAGGACCTG
TGGACGCAACCGCCCAAGCCCTACACCGAAGGCGACCTGATCAAGGCGATGAAGAACGTCGCCAAACTGGTGGAAGATCC
GCTGCTCAAGCAGAAACTCAAGGACACCACCGGCATCGGCACCGAAGCCACCCGCGCGTCGATCATTCAAGGGTTGCTGG
ATCGCGGCTATCTGGTGAAAAACGGCAAGGCGCTGTCCGCCACGCCCGCCGCGTTCAGCCTGATCGACGCCGTACCGCGC
GCGATTGCCGACCCCGGCACCACGGCGATCTGGGAGCAGGCGCTGGACATGGTGCAGAGCGGCGAAATGAGTCTGGAAGA
ATTCGTCACCAAACAGGCCGCGTGGATGAGCAAGCAAGTGACCCGCTGCGCCGGACTGAGCCTGACCATCAGCGGCCCGC
CACCTGCCGGCAAGGCCGCTGCGCCATGGAAGAACAAACGCAAGACCACTCGGCGCAAAACCACCGGAACCAGCAAACGC
GTGGCGAAACCAGCAGCCAAATAG

Upstream 100 bases:

>100_bases
CAATCCTTTTATCTGTATATCCATACAGATAAAAGTTGCCCGAACGCCCGAGTCAGGCCATTCTGTGCGCCTCTTCGGGC
AATGATCGACGACGGTGGTT

Downstream 100 bases:

>100_bases
CACGCTGCACGCTACTGTTTCTGCAGTGATGGTCAGGAGGCAGCGGCATGTCGTTTATCGAACTGCGCGTCGCCCGGCGC
GACGAACTGGACACCCTCGA

Product: DNA topoisomerase III

Products: NA

Alternate protein names: DNA topoisomerase III [H]

Number of amino acids: Translated: 647; Mature: 647

Protein sequence:

>647_residues
MRLYLCEKPSQAKDIAAVLGAKRRGDGCWLGTDVTVTWCIGHLLETAPPDAYDAKYKRWVLADLPIIPDKWKMTVKPRTA
SQYKAVKRLLGEASELIIATDADREGEMIARELVEHCRYRGPIRRLWLSALDEASIRKALAALKPGAETFSLYHSALGRS
RADWLIGMNMSRLFTLLGRQSGYQGVLPVGRVQTPTLRLVVDRDRSIADFVPVAYWAIDVQLLHGDTPFNAQWRAPNDAC
DDQDRCLNQALAQQAAAAISSAASARVVKLRTERMREVAPLPFDLGTLQEVCSKKLGLGAQETLDIAQALYETYKVITYP
RSDCGYLPLSQHNEAPGILAALRQADPSLEALREHLEPQRRSRAWNDAKVSAHHGIIPTAAAKNLERLTGKHRAVYTLIR
ARYLAQFLPNHEYDRTQADFDCAGEALRAVGKQIIEPGWKRALPEALAPAKGREAPAPQTLPTLTQGAECAVADVKLKDL
WTQPPKPYTEGDLIKAMKNVAKLVEDPLLKQKLKDTTGIGTEATRASIIQGLLDRGYLVKNGKALSATPAAFSLIDAVPR
AIADPGTTAIWEQALDMVQSGEMSLEEFVTKQAAWMSKQVTRCAGLSLTISGPPPAGKAAAPWKNKRKTTRRKTTGTSKR
VAKPAAK

Sequences:

>Translated_647_residues
MRLYLCEKPSQAKDIAAVLGAKRRGDGCWLGTDVTVTWCIGHLLETAPPDAYDAKYKRWVLADLPIIPDKWKMTVKPRTA
SQYKAVKRLLGEASELIIATDADREGEMIARELVEHCRYRGPIRRLWLSALDEASIRKALAALKPGAETFSLYHSALGRS
RADWLIGMNMSRLFTLLGRQSGYQGVLPVGRVQTPTLRLVVDRDRSIADFVPVAYWAIDVQLLHGDTPFNAQWRAPNDAC
DDQDRCLNQALAQQAAAAISSAASARVVKLRTERMREVAPLPFDLGTLQEVCSKKLGLGAQETLDIAQALYETYKVITYP
RSDCGYLPLSQHNEAPGILAALRQADPSLEALREHLEPQRRSRAWNDAKVSAHHGIIPTAAAKNLERLTGKHRAVYTLIR
ARYLAQFLPNHEYDRTQADFDCAGEALRAVGKQIIEPGWKRALPEALAPAKGREAPAPQTLPTLTQGAECAVADVKLKDL
WTQPPKPYTEGDLIKAMKNVAKLVEDPLLKQKLKDTTGIGTEATRASIIQGLLDRGYLVKNGKALSATPAAFSLIDAVPR
AIADPGTTAIWEQALDMVQSGEMSLEEFVTKQAAWMSKQVTRCAGLSLTISGPPPAGKAAAPWKNKRKTTRRKTTGTSKR
VAKPAAK
>Mature_647_residues
MRLYLCEKPSQAKDIAAVLGAKRRGDGCWLGTDVTVTWCIGHLLETAPPDAYDAKYKRWVLADLPIIPDKWKMTVKPRTA
SQYKAVKRLLGEASELIIATDADREGEMIARELVEHCRYRGPIRRLWLSALDEASIRKALAALKPGAETFSLYHSALGRS
RADWLIGMNMSRLFTLLGRQSGYQGVLPVGRVQTPTLRLVVDRDRSIADFVPVAYWAIDVQLLHGDTPFNAQWRAPNDAC
DDQDRCLNQALAQQAAAAISSAASARVVKLRTERMREVAPLPFDLGTLQEVCSKKLGLGAQETLDIAQALYETYKVITYP
RSDCGYLPLSQHNEAPGILAALRQADPSLEALREHLEPQRRSRAWNDAKVSAHHGIIPTAAAKNLERLTGKHRAVYTLIR
ARYLAQFLPNHEYDRTQADFDCAGEALRAVGKQIIEPGWKRALPEALAPAKGREAPAPQTLPTLTQGAECAVADVKLKDL
WTQPPKPYTEGDLIKAMKNVAKLVEDPLLKQKLKDTTGIGTEATRASIIQGLLDRGYLVKNGKALSATPAAFSLIDAVPR
AIADPGTTAIWEQALDMVQSGEMSLEEFVTKQAAWMSKQVTRCAGLSLTISGPPPAGKAAAPWKNKRKTTRRKTTGTSKR
VAKPAAK

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another. TOP3 is a potent decatenase [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=568, Percent_Identity=25.5281690140845, Blast_Score=121, Evalue=3e-27,
Organism=Homo sapiens, GI4507635, Length=623, Percent_Identity=24.5585874799358, Blast_Score=114, Evalue=2e-25,
Organism=Escherichia coli, GI1788061, Length=601, Percent_Identity=40.432612312812, Blast_Score=429, Evalue=1e-121,
Organism=Escherichia coli, GI1787529, Length=630, Percent_Identity=26.1904761904762, Blast_Score=136, Evalue=3e-33,
Organism=Caenorhabditis elegans, GI17555378, Length=641, Percent_Identity=24.4929797191888, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI32563869, Length=623, Percent_Identity=24.7191011235955, Blast_Score=119, Evalue=4e-27,
Organism=Saccharomyces cerevisiae, GI6323263, Length=625, Percent_Identity=26.24, Blast_Score=105, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24585251, Length=628, Percent_Identity=26.7515923566879, Blast_Score=125, Evalue=1e-28,
Organism=Drosophila melanogaster, GI24640096, Length=623, Percent_Identity=24.0770465489567, Blast_Score=122, Evalue=6e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR005738
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 71090; Mature: 71090

Theoretical pI: Translated: 9.85; Mature: 9.85

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLYLCEKPSQAKDIAAVLGAKRRGDGCWLGTDVTVTWCIGHLLETAPPDAYDAKYKRWV
CEEEEECCCCHHHHHHHHHCCCCCCCCCEECCCHHHHHHHHHHHHCCCCCCCCCHHHHEE
LADLPIIPDKWKMTVKPRTASQYKAVKRLLGEASELIIATDADREGEMIARELVEHCRYR
EECCCCCCCCCEEEECCCCHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHCC
GPIRRLWLSALDEASIRKALAALKPGAETFSLYHSALGRSRADWLIGMNMSRLFTLLGRQ
CHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHCC
SGYQGVLPVGRVQTPTLRLVVDRDRSIADFVPVAYWAIDVQLLHGDTPFNAQWRAPNDAC
CCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHEEEEEEECCCCCCCCCCCCCCCC
DDQDRCLNQALAQQAAAAISSAASARVVKLRTERMREVAPLPFDLGTLQEVCSKKLGLGA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCH
QETLDIAQALYETYKVITYPRSDCGYLPLSQHNEAPGILAALRQADPSLEALREHLEPQR
HHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHCHHH
RSRAWNDAKVSAHHGIIPTAAAKNLERLTGKHRAVYTLIRARYLAQFLPNHEYDRTQADF
HHCCCCCCCCCCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCH
DCAGEALRAVGKQIIEPGWKRALPEALAPAKGREAPAPQTLPTLTQGAECAVADVKLKDL
HHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCCEEEEEEEHHHH
WTQPPKPYTEGDLIKAMKNVAKLVEDPLLKQKLKDTTGIGTEATRASIIQGLLDRGYLVK
HCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCEEEE
NGKALSATPAAFSLIDAVPRAIADPGTTAIWEQALDMVQSGEMSLEEFVTKQAAWMSKQV
CCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
TRCAGLSLTISGPPPAGKAAAPWKNKRKTTRRKTTGTSKRVAKPAAK
HHHCCCEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHCCCCCC
>Mature Secondary Structure
MRLYLCEKPSQAKDIAAVLGAKRRGDGCWLGTDVTVTWCIGHLLETAPPDAYDAKYKRWV
CEEEEECCCCHHHHHHHHHCCCCCCCCCEECCCHHHHHHHHHHHHCCCCCCCCCHHHHEE
LADLPIIPDKWKMTVKPRTASQYKAVKRLLGEASELIIATDADREGEMIARELVEHCRYR
EECCCCCCCCCEEEECCCCHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHCC
GPIRRLWLSALDEASIRKALAALKPGAETFSLYHSALGRSRADWLIGMNMSRLFTLLGRQ
CHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCEEECCCHHHHHHHHHCC
SGYQGVLPVGRVQTPTLRLVVDRDRSIADFVPVAYWAIDVQLLHGDTPFNAQWRAPNDAC
CCCCCCCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHEEEEEEECCCCCCCCCCCCCCCC
DDQDRCLNQALAQQAAAAISSAASARVVKLRTERMREVAPLPFDLGTLQEVCSKKLGLGA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCH
QETLDIAQALYETYKVITYPRSDCGYLPLSQHNEAPGILAALRQADPSLEALREHLEPQR
HHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHCHHH
RSRAWNDAKVSAHHGIIPTAAAKNLERLTGKHRAVYTLIRARYLAQFLPNHEYDRTQADF
HHCCCCCCCCCCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCH
DCAGEALRAVGKQIIEPGWKRALPEALAPAKGREAPAPQTLPTLTQGAECAVADVKLKDL
HHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCCEEEEEEEHHHH
WTQPPKPYTEGDLIKAMKNVAKLVEDPLLKQKLKDTTGIGTEATRASIIQGLLDRGYLVK
HCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCEEEE
NGKALSATPAAFSLIDAVPRAIADPGTTAIWEQALDMVQSGEMSLEEFVTKQAAWMSKQV
CCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
TRCAGLSLTISGPPPAGKAAAPWKNKRKTTRRKTTGTSKRVAKPAAK
HHHCCCEEEECCCCCCCCCCCCCCHHHHHHHHHCCCCHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]