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The map label for this gene is ydaP [H]

Identifier: 77458957

GI number: 77458957

Start: 3148910

End: 3150700

Strand: Direct

Name: ydaP [H]

Synonym: Pfl01_2732

Alternate gene names: 77458957

Gene position: 3148910-3150700 (Clockwise)

Preceding gene: 77458956

Following gene: 77458960

Centisome position: 48.91

GC content: 60.69

Gene sequence:

>1791_bases
ATGACGGTGACAGTGGGTGATTTTCTGGTCGAACGCCTGAGCCAATGGGGTGTGACGCGCATTTTTGGTTATCCGGGCGA
CGGTATCAACGGCGTCTTCGGTACGTTGAGCCGGGCAGGTGGCAAGATCGAATTCGTCCAGGCGCGTCACGAGGAAATGG
CGGCGTTCATGGCGTCCGCCCATGCCAAATTCACTGGTGAACTGGGTGTGTGCATCGCGACATCCGGCCCCGGCGCCTCG
CACCTGATCACCGGACTGTACGACGCTCGGCTGGACCACATGCCGGTGCTGGCGATTGTCGGGCAGCAGGCGCGCACGGC
ACTGGGCAGCCATTATCAGCAAGAGCTGGATCTGGTCTCGATGTTCAAGGACGTGGCCGGAGCATTTGTGCAACAGGCCT
CGGCGCCTTCCCAGGTCCGGCACTTGCTGGATCGTGCGGTGCGCACGGCGATTGGCGAACGCCGGGTGACTGCGTTGATC
CTGCCGAACGACCTGCAGGACTTAGAGTACAAGGCGCCGGCGAGGGCCCACGGCACTGCGCATTCGGGGGTTGGTTACAG
CAAGCCGAAAGTGCTGCCTTATGACGCGGATCTGCAACGTGCGGCCGATGTGCTGAATGCGGGAGAGAAGGTTGCGATTC
TGGTGGGTGCCGGAGCGCTGCACGCCAGCGAGCAAGTGTTTGCGATTGCCGAGAAACTGGGCGCCGGAGTGGCAAAGGCA
CTGCTCGGCAAAGCTGTATTACCGGACGAATTGCCTTGGGTCACAGGTTGTATCGGTCTGCTCGGCACCGAACCGAGCTA
CAAATTGATGACGGAGTGCGACACCCTGCTGATGATCGGATCGGGGTTTCCTTATGCCGAGTTCTTGCCGAAAGAAGGGC
AGGCGCGCGGGGTTCAGATCGACCTGCAACCGGACATGCTCAGTCTGCGCTATCCGATGGAGGTGAACCTTGCCGGCGAT
GCGGCCGAGACCCTGGCGGCACTGGCACCGTTGCTCGAACACAAGCCACTGCGCAAGTGGCGTAAAAAGATCGAAGGCTG
GCGGGCGAGCTGGGATAAAACGCTCGAGAAGCGCGCGATGGCCAAGGCCGACCCGGTTAACCCGCAACGGGTGGTGTATG
AGTTGTCCCCGCGTCTGCCCGAGCAGGCCATCATCACCAGCGACTCCGGTTCTTGCGCCAACTGGTTCGCCCGTGACCTG
AAAATCCGCAAGGGCATGAAGTGCTCGCTCTCCGGTGGCCTGGCCTCGATGGGCGCCGCTGTGCCATACGCGATCGCGGC
GAAGTTCGCTTTCCCGCAACGGCCGGTGATCGCCCTGGTGGGCGATGGCGCCATGCAGATGAACAACATGGCCGAACTGA
TAACGGTCGCCAAATACTGGCGACAGTGGGACAGCCCGAAGTGGATCTGCGCCGTGTTCAACAATGAGGATCTGAATCAG
GTCACCTGGGAACAGCGCGTGATGGAGGGCGATCCGAAATTCGGTGCCTCGCAGAGTATTCCGGATGTGCCATACCATTT
GTTCGCCATCTCCATCGGCCTCAAAGGCATTTTTGTCGATCGGGAGGAAGACGTGGCCGCAGCCTGGGAGCAGGCGTTAG
CTTCCGAAGTACCGGTCGTCATTGAATTCAAGACCGACCCGAATGTGCCGCCGTTGCCGCCGCATATCAAGCTGGAACAG
GCAAAGAAATTCGCCAGCACGTTGCTCAAGGGTGATCCGGACGAAGCGGGCGTCATCGTGCAAACGGCTAAACAGGTATT
GGGGACGGTATTGCCCGGGAAGCGCAAGTAG

Upstream 100 bases:

>100_bases
TTCGCTGTCCGCCGGGCGGTCCAGACCTTTACATCTCCCGAGCGTGCTGTGGATGTCGGTGCACCGGGATGTCATACGCA
ATGAAAAGATGAGGTCGGAC

Downstream 100 bases:

>100_bases
TGACGGTAAGACCCGCGAAGAGTTTCGTGACGATGGCTTGGTTTTCAATCTGCATATCACCAGGACGCCGGGGAGCCAGG
GCGTTCTGGCGGCAAAGGTG

Product: thiamine pyrophosphate protein

Products: Acetate; CO2; Ubiquinol-8 [C]

Alternate protein names: NA

Number of amino acids: Translated: 596; Mature: 595

Protein sequence:

>596_residues
MTVTVGDFLVERLSQWGVTRIFGYPGDGINGVFGTLSRAGGKIEFVQARHEEMAAFMASAHAKFTGELGVCIATSGPGAS
HLITGLYDARLDHMPVLAIVGQQARTALGSHYQQELDLVSMFKDVAGAFVQQASAPSQVRHLLDRAVRTAIGERRVTALI
LPNDLQDLEYKAPARAHGTAHSGVGYSKPKVLPYDADLQRAADVLNAGEKVAILVGAGALHASEQVFAIAEKLGAGVAKA
LLGKAVLPDELPWVTGCIGLLGTEPSYKLMTECDTLLMIGSGFPYAEFLPKEGQARGVQIDLQPDMLSLRYPMEVNLAGD
AAETLAALAPLLEHKPLRKWRKKIEGWRASWDKTLEKRAMAKADPVNPQRVVYELSPRLPEQAIITSDSGSCANWFARDL
KIRKGMKCSLSGGLASMGAAVPYAIAAKFAFPQRPVIALVGDGAMQMNNMAELITVAKYWRQWDSPKWICAVFNNEDLNQ
VTWEQRVMEGDPKFGASQSIPDVPYHLFAISIGLKGIFVDREEDVAAAWEQALASEVPVVIEFKTDPNVPPLPPHIKLEQ
AKKFASTLLKGDPDEAGVIVQTAKQVLGTVLPGKRK

Sequences:

>Translated_596_residues
MTVTVGDFLVERLSQWGVTRIFGYPGDGINGVFGTLSRAGGKIEFVQARHEEMAAFMASAHAKFTGELGVCIATSGPGAS
HLITGLYDARLDHMPVLAIVGQQARTALGSHYQQELDLVSMFKDVAGAFVQQASAPSQVRHLLDRAVRTAIGERRVTALI
LPNDLQDLEYKAPARAHGTAHSGVGYSKPKVLPYDADLQRAADVLNAGEKVAILVGAGALHASEQVFAIAEKLGAGVAKA
LLGKAVLPDELPWVTGCIGLLGTEPSYKLMTECDTLLMIGSGFPYAEFLPKEGQARGVQIDLQPDMLSLRYPMEVNLAGD
AAETLAALAPLLEHKPLRKWRKKIEGWRASWDKTLEKRAMAKADPVNPQRVVYELSPRLPEQAIITSDSGSCANWFARDL
KIRKGMKCSLSGGLASMGAAVPYAIAAKFAFPQRPVIALVGDGAMQMNNMAELITVAKYWRQWDSPKWICAVFNNEDLNQ
VTWEQRVMEGDPKFGASQSIPDVPYHLFAISIGLKGIFVDREEDVAAAWEQALASEVPVVIEFKTDPNVPPLPPHIKLEQ
AKKFASTLLKGDPDEAGVIVQTAKQVLGTVLPGKRK
>Mature_595_residues
TVTVGDFLVERLSQWGVTRIFGYPGDGINGVFGTLSRAGGKIEFVQARHEEMAAFMASAHAKFTGELGVCIATSGPGASH
LITGLYDARLDHMPVLAIVGQQARTALGSHYQQELDLVSMFKDVAGAFVQQASAPSQVRHLLDRAVRTAIGERRVTALIL
PNDLQDLEYKAPARAHGTAHSGVGYSKPKVLPYDADLQRAADVLNAGEKVAILVGAGALHASEQVFAIAEKLGAGVAKAL
LGKAVLPDELPWVTGCIGLLGTEPSYKLMTECDTLLMIGSGFPYAEFLPKEGQARGVQIDLQPDMLSLRYPMEVNLAGDA
AETLAALAPLLEHKPLRKWRKKIEGWRASWDKTLEKRAMAKADPVNPQRVVYELSPRLPEQAIITSDSGSCANWFARDLK
IRKGMKCSLSGGLASMGAAVPYAIAAKFAFPQRPVIALVGDGAMQMNNMAELITVAKYWRQWDSPKWICAVFNNEDLNQV
TWEQRVMEGDPKFGASQSIPDVPYHLFAISIGLKGIFVDREEDVAAAWEQALASEVPVVIEFKTDPNVPPLPPHIKLEQA
KKFASTLLKGDPDEAGVIVQTAKQVLGTVLPGKRK

Specific function: Unknown

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=576, Percent_Identity=23.0902777777778, Blast_Score=113, Evalue=4e-25,
Organism=Escherichia coli, GI1787096, Length=571, Percent_Identity=33.0998248686515, Blast_Score=267, Evalue=1e-72,
Organism=Escherichia coli, GI87081685, Length=554, Percent_Identity=29.4223826714801, Blast_Score=188, Evalue=7e-49,
Organism=Escherichia coli, GI1790104, Length=568, Percent_Identity=26.9366197183099, Blast_Score=183, Evalue=2e-47,
Organism=Escherichia coli, GI1786717, Length=564, Percent_Identity=26.9503546099291, Blast_Score=135, Evalue=7e-33,
Organism=Escherichia coli, GI1788716, Length=484, Percent_Identity=23.7603305785124, Blast_Score=81, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI17531299, Length=585, Percent_Identity=25.6410256410256, Blast_Score=89, Evalue=6e-18,
Organism=Caenorhabditis elegans, GI17531301, Length=585, Percent_Identity=25.6410256410256, Blast_Score=88, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6323755, Length=575, Percent_Identity=25.7391304347826, Blast_Score=157, Evalue=3e-39,
Organism=Saccharomyces cerevisiae, GI6320816, Length=580, Percent_Identity=21.7241379310345, Blast_Score=87, Evalue=8e-18,
Organism=Drosophila melanogaster, GI19922626, Length=569, Percent_Identity=23.9015817223199, Blast_Score=104, Evalue=2e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: 1.2.2.2 [C]

Molecular weight: Translated: 64518; Mature: 64387

Theoretical pI: Translated: 7.14; Mature: 7.14

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVTVGDFLVERLSQWGVTRIFGYPGDGINGVFGTLSRAGGKIEFVQARHEEMAAFMASA
CEEEHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHCCCEEEEHHHHHHHHHHHHHHH
HAKFTGELGVCIATSGPGASHLITGLYDARLDHMPVLAIVGQQARTALGSHYQQELDLVS
HHCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCHHHHHCHHHHHHHHHHHHHHHHHHH
MFKDVAGAFVQQASAPSQVRHLLDRAVRTAIGERRVTALILPNDLQDLEYKAPARAHGTA
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCC
HSGVGYSKPKVLPYDADLQRAADVLNAGEKVAILVGAGALHASEQVFAIAEKLGAGVAKA
CCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHH
LLGKAVLPDELPWVTGCIGLLGTEPSYKLMTECDTLLMIGSGFPYAEFLPKEGQARGVQI
HHCCCCCCCCCCHHHHHHHHHCCCCCHHHHHCCCEEEEEECCCCHHHHCCCCCCCCCEEE
DLQPDMLSLRYPMEVNLAGDAAETLAALAPLLEHKPLRKWRKKIEGWRASWDKTLEKRAM
EECCCCEEEECCEEEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AKADPVNPQRVVYELSPRLPEQAIITSDSGSCANWFARDLKIRKGMKCSLSGGLASMGAA
HCCCCCCHHHEEEECCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCEEECCCCHHHHHHH
VPYAIAAKFAFPQRPVIALVGDGAMQMNNMAELITVAKYWRQWDSPKWICAVFNNEDLNQ
HHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHH
VTWEQRVMEGDPKFGASQSIPDVPYHLFAISIGLKGIFVDREEDVAAAWEQALASEVPVV
HHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCEEEEECCCHHHHHHHHHHHHCCCCEE
IEFKTDPNVPPLPPHIKLEQAKKFASTLLKGDPDEAGVIVQTAKQVLGTVLPGKRK
EEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TVTVGDFLVERLSQWGVTRIFGYPGDGINGVFGTLSRAGGKIEFVQARHEEMAAFMASA
EEEHHHHHHHHHHHCCCEEEECCCCCCCCHHHHHHHHCCCEEEEHHHHHHHHHHHHHHH
HAKFTGELGVCIATSGPGASHLITGLYDARLDHMPVLAIVGQQARTALGSHYQQELDLVS
HHCCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCCHHHHHCHHHHHHHHHHHHHHHHHHH
MFKDVAGAFVQQASAPSQVRHLLDRAVRTAIGERRVTALILPNDLQDLEYKAPARAHGTA
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCCCC
HSGVGYSKPKVLPYDADLQRAADVLNAGEKVAILVGAGALHASEQVFAIAEKLGAGVAKA
CCCCCCCCCCCCCCCCHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHH
LLGKAVLPDELPWVTGCIGLLGTEPSYKLMTECDTLLMIGSGFPYAEFLPKEGQARGVQI
HHCCCCCCCCCCHHHHHHHHHCCCCCHHHHHCCCEEEEEECCCCHHHHCCCCCCCCCEEE
DLQPDMLSLRYPMEVNLAGDAAETLAALAPLLEHKPLRKWRKKIEGWRASWDKTLEKRAM
EECCCCEEEECCEEEEECCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
AKADPVNPQRVVYELSPRLPEQAIITSDSGSCANWFARDLKIRKGMKCSLSGGLASMGAA
HCCCCCCHHHEEEECCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCEEECCCCHHHHHHH
VPYAIAAKFAFPQRPVIALVGDGAMQMNNMAELITVAKYWRQWDSPKWICAVFNNEDLNQ
HHHHHHHHHCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHH
VTWEQRVMEGDPKFGASQSIPDVPYHLFAISIGLKGIFVDREEDVAAAWEQALASEVPVV
HHHHHHHHCCCCCCCCCCCCCCCCCEEEEEEECCEEEEECCCHHHHHHHHHHHHCCCCEE
IEFKTDPNVPPLPPHIKLEQAKKFASTLLKGDPDEAGVIVQTAKQVLGTVLPGKRK
EEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FAD oxidase,; Lipids site,; Phospholipids; Thiamine diphosphate [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): 87000 [C]

Specific activity: 200

Km value (mM): 0.0022 {lysolecithin}} 0.0017 {lecithin}} 0.0012 {thiamine} 0.03 {sodium} 2 {ferricyanide}} 11 {pyruvate}} 10 {pyruvate}} [C]

Substrates: H2O; Pyruvate; Ubiquinone-8 [C]

Specific reaction: H2O + Pyruvate + Ubiquinone-8 --> Acetate + CO2 + Ubiquinol-8 [C]

General reaction: Oxidative decarboxylation; Redox reaction [C]

Inhibitor: 2, 3-Butanedione; Cu2+; Methyl methanethio sulfonate; N-Ethyl maleimide diphosphate; p-Chloromercuribenzoate; Phenyl glyoxal [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]