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The map label for this gene is cynT [H]

Identifier: 77458956

GI number: 77458956

Start: 3147858

End: 3148517

Strand: Direct

Name: cynT [H]

Synonym: Pfl01_2731

Alternate gene names: 77458956

Gene position: 3147858-3148517 (Clockwise)

Preceding gene: 77458955

Following gene: 77458957

Centisome position: 48.89

GC content: 58.18

Gene sequence:

>660_bases
ATGCGTGACATTATTGATGGCTTTCTGCGTTTTCAACGCGAGGTGTATCCACAACGCGTCGAGCTTTTCAGGCAGCTCGC
GACAGAGCAAAACCCAAAGGCGTTGTTCGTAACATGCTCGGACAGTCGTGTAGTGCCTGAACTGCTCACACAGCGGGAGC
CGGGTGAGCTGTTCGTGATCCGCAACGCCGGCAACATTGTGCCATCGTATGGCCCTGAACCCGGTGGTGTTTCGGCCACC
GTCGAATATGCGGTTGCAGTGCTCGGCGTACGGGACATCGTGATCTGCGGCCACTCGGATTGCGGAGCGATGGGCGCGAT
TTCACGCTGCACGTGTCTGGATCATCTTCCGGCAGTTGCCAACTGGCTTCGTCACTCGGACGCCGCGAAAGCGATCAACG
CGGCGCATGAGTTCGATACGCCACGCGACAAGCTCGATGGCCTCGTTCGTGAAAACGTGATCGCACAACTGGCGAACCTG
CGGACTCATCCATCGGTGGCGCTTGCGCTTGAGCAAGGACGCTTGAATTTGCATGGCTGGGTCTATGACATCGAGGCAGG
TTGCATCGATGCGCTGGATGGAGCCACTCGACGCTTCGTTCCACTGTCCGATTCACCGACCACCGTTGCCGTCGCTGCGC
GCAACGGTGGTCAGATATAA

Upstream 100 bases:

>100_bases
CGCGCACTCGCATGCGACGTCTGCAATAATGACGCCCCACTGAGACGCGAAGGCATCAAGTGCTCGTCTCGGCATCCGGA
ATCAGGTTTTGGAGGACTCC

Downstream 100 bases:

>100_bases
CGGCGACAGACAGGTCGGGACGGGGAAAATTTCGTCCCGGCCGATCGGTCCCTGGCCCGAATCAAAGACCCACCGATGAC
TCACAATCTTTTGATATTTC

Product: carbonate dehydratase

Products: NA

Alternate protein names: Carbonate dehydratase [H]

Number of amino acids: Translated: 219; Mature: 219

Protein sequence:

>219_residues
MRDIIDGFLRFQREVYPQRVELFRQLATEQNPKALFVTCSDSRVVPELLTQREPGELFVIRNAGNIVPSYGPEPGGVSAT
VEYAVAVLGVRDIVICGHSDCGAMGAISRCTCLDHLPAVANWLRHSDAAKAINAAHEFDTPRDKLDGLVRENVIAQLANL
RTHPSVALALEQGRLNLHGWVYDIEAGCIDALDGATRRFVPLSDSPTTVAVAARNGGQI

Sequences:

>Translated_219_residues
MRDIIDGFLRFQREVYPQRVELFRQLATEQNPKALFVTCSDSRVVPELLTQREPGELFVIRNAGNIVPSYGPEPGGVSAT
VEYAVAVLGVRDIVICGHSDCGAMGAISRCTCLDHLPAVANWLRHSDAAKAINAAHEFDTPRDKLDGLVRENVIAQLANL
RTHPSVALALEQGRLNLHGWVYDIEAGCIDALDGATRRFVPLSDSPTTVAVAARNGGQI
>Mature_219_residues
MRDIIDGFLRFQREVYPQRVELFRQLATEQNPKALFVTCSDSRVVPELLTQREPGELFVIRNAGNIVPSYGPEPGGVSAT
VEYAVAVLGVRDIVICGHSDCGAMGAISRCTCLDHLPAVANWLRHSDAAKAINAAHEFDTPRDKLDGLVRENVIAQLANL
RTHPSVALALEQGRLNLHGWVYDIEAGCIDALDGATRRFVPLSDSPTTVAVAARNGGQI

Specific function: Reversible Hydration Of Carbon Dioxide. Carbon Dioxide Formed In The Bicarbonate-Dependent Decomposition Of Cyanate By Cyanase (Cyns) Diffuses Out Of The Cell Faster Than It Would Be Hydrated To Bicarbonate, So The Apparent Function Of This Enzyme Is To C

COG id: COG0288

COG function: function code P; Carbonic anhydrase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the beta-class carbonic anhydrase family [H]

Homologues:

Organism=Escherichia coli, GI1786534, Length=215, Percent_Identity=67.4418604651163, Blast_Score=317, Evalue=3e-88,
Organism=Escherichia coli, GI1786318, Length=180, Percent_Identity=37.7777777777778, Blast_Score=99, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI25147564, Length=253, Percent_Identity=30.8300395256917, Blast_Score=116, Evalue=9e-27,
Organism=Caenorhabditis elegans, GI115532990, Length=239, Percent_Identity=28.8702928870293, Blast_Score=102, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6324292, Length=213, Percent_Identity=29.1079812206573, Blast_Score=86, Evalue=7e-18,
Organism=Drosophila melanogaster, GI24645213, Length=245, Percent_Identity=26.9387755102041, Blast_Score=98, Evalue=5e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001765
- InterPro:   IPR015892 [H]

Pfam domain/function: PF00484 Pro_CA [H]

EC number: =4.2.1.1 [H]

Molecular weight: Translated: 23796; Mature: 23796

Theoretical pI: Translated: 5.94; Mature: 5.94

Prosite motif: PS00704 PROK_CO2_ANHYDRASE_1 ; PS00705 PROK_CO2_ANHYDRASE_2 ; PS00228 TUBULIN_B_AUTOREG

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRDIIDGFLRFQREVYPQRVELFRQLATEQNPKALFVTCSDSRVVPELLTQREPGELFVI
CHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHCCCCCCCEEEE
RNAGNIVPSYGPEPGGVSATVEYAVAVLGVRDIVICGHSDCGAMGAISRCTCLDHLPAVA
ECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHH
NWLRHSDAAKAINAAHEFDTPRDKLDGLVRENVIAQLANLRTHPSVALALEQGRLNLHGW
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCEEEEEE
VYDIEAGCIDALDGATRRFVPLSDSPTTVAVAARNGGQI
EEEECCCHHHHHCCCCEEEEECCCCCCEEEEEECCCCCC
>Mature Secondary Structure
MRDIIDGFLRFQREVYPQRVELFRQLATEQNPKALFVTCSDSRVVPELLTQREPGELFVI
CHHHHHHHHHHHHHHCHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHCCCCCCCEEEE
RNAGNIVPSYGPEPGGVSATVEYAVAVLGVRDIVICGHSDCGAMGAISRCTCLDHLPAVA
ECCCCCCCCCCCCCCCCHHHHHHHHHHHHHHEEEEECCCCCCCHHHHHHHHHHHHHHHHH
NWLRHSDAAKAINAAHEFDTPRDKLDGLVRENVIAQLANLRTHPSVALALEQGRLNLHGW
HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCEEEEEE
VYDIEAGCIDALDGATRRFVPLSDSPTTVAVAARNGGQI
EEEECCCHHHHHCCCCEEEEECCCCCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]