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The map label for this gene is oprM [H]

Identifier: 77458749

GI number: 77458749

Start: 2885554

End: 2887023

Strand: Direct

Name: oprM [H]

Synonym: Pfl01_2524

Alternate gene names: 77458749

Gene position: 2885554-2887023 (Clockwise)

Preceding gene: 77458748

Following gene: 77458750

Centisome position: 44.82

GC content: 61.97

Gene sequence:

>1470_bases
ATGACTGACCGTTCGCTTATCAATCTGGCTACCGTTCGCGGCTCGCGTCTGCTGAGCCTGTCGCTGTGCGTGGCGATGCT
CAGTGCCTGCGCCGTCGGCCCGGACTACCAGCGCCCGCAGACCGCCGAAATCGCCCAGTACAAGGAAGCCGAAGGCTGGC
GTCAGGCCAATCCGAGCGATTCCCTGGCGCGTGGTGCCTGGTGGGAGCTGTATGGCGATCGTCAGCTCAACCAGCTGATC
GAGAAACTCAACAGTTCGAACCAGACCGTCGCCCAGTCCGAGGCCCAGTACCGTCAGGCCCAGGCGTTGGTACGCAGTGC
ACGCGGGGCGTTTTATCCGAGCGTCGATCTGAGTGCCGGCAAGACCCGCTCCAGCCAGGGCACCGGCAGCAGCAGTTCGA
GTCTGAGCAGTTCCTCCAGCGGCATTCGTGACACGTACAACGCGCAACTGGGCGTGAGTTGGGAAGCGGATGTCTGGGGC
AAGTTGCGTCGGGGCCTGGAAGCCAATGAAGCCAGTGCCCAGGCGAGCTACGCCGATCTGGCGGCGATGCGTCTGAGCCA
GCAGTCGGAACTGGTGCAGAACTACCTGCAACTGCGAGTGATTGATCAGCAGAAACGCCTGCTCGAATCGACAGTGGCGG
CGTACGAGCGCTCGCTGAAGATGACCATGAACCAGTACAACGCCGGGGTTTCCGGACGTGATGCGGTGGCGCAGGCGCAG
ACCCAGCTGAAAACCACCCAGGGCGATCTGGTGGACCTGATCTGGCAACGGGCGCAGTTCGAAAACGCCATCGCGGTACT
GACCGGCCAGCCGCCCGCTGAATTCAACATTGCCGAAACTCAGGACATTCCCAAGCTGCCGCAGATTCCACTGAGCCTGC
CGTCGCAGTTGCTCGAGCGCCGTCCGGACATCGCCTCGGCCGAGCGCTCGGTGATCGCCGCCAACGCCAACATCGGCGTG
GCCAAGGCTGCGTATTACCCGGATTTCAGCCTGAGCATGAGCGGCGGTTACAGCAGCAGTACCTCGCAGAACCTGATCAG
CCTGCCGAACCGCTTCTGGTCGGTGGGGCCGAAAATGACCCTGCCGCTGTTTGACGGCGGCATTCGTTCGGCCGAAGTCG
ACCGCACCGAAGCCGTGTACGACCAGACCGTGGCCAAATACCGCCAGACTGTGCTCGATGGTTTCCGCGAAGTGGAAAAC
TATCTGGTGCAGTTGAAGGTTTACGAAGATGAAGCGGCCGTGCGCCAGGAAGCCCTCGACGCGGCACGGGATTCGTTGCG
TCTGACCGAGAACCAGTACAAGGCCGGGGTGATTGCTTATCTCGATGTAGTGACGGTGCAGGCGACGGCGCTGAGCAATG
AACGCACGGTGCTGAATATCCTGCAGAGCCGTTTGATTGCCAGTGTGCAGCTGATTGCGGCGCTGGGTGGTGGCTGGGAC
GGGCAGCTGGAAACCACTGATAACCGCTGA

Upstream 100 bases:

>100_bases
TTTACACCACGCCTGTGGTTTACCTCTATCTCGACAAGCTGCGCCATCGTTTCAACAAATGGCGTGGCGTGCGCACTGAT
GCCGCTCTGGAAACTCCGCT

Downstream 100 bases:

>100_bases
AACAGAGCCGGGAGCGATCTGCTCAAGACACCGAAATTGAGCCTTGTCAGACGATCAAACAAGCGTTTCATCGGCTTGAT
GGCCATTTGATTACTTTGTC

Product: RND efflux system outer membrane lipoprotein NodT

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 489; Mature: 488

Protein sequence:

>489_residues
MTDRSLINLATVRGSRLLSLSLCVAMLSACAVGPDYQRPQTAEIAQYKEAEGWRQANPSDSLARGAWWELYGDRQLNQLI
EKLNSSNQTVAQSEAQYRQAQALVRSARGAFYPSVDLSAGKTRSSQGTGSSSSSLSSSSSGIRDTYNAQLGVSWEADVWG
KLRRGLEANEASAQASYADLAAMRLSQQSELVQNYLQLRVIDQQKRLLESTVAAYERSLKMTMNQYNAGVSGRDAVAQAQ
TQLKTTQGDLVDLIWQRAQFENAIAVLTGQPPAEFNIAETQDIPKLPQIPLSLPSQLLERRPDIASAERSVIAANANIGV
AKAAYYPDFSLSMSGGYSSSTSQNLISLPNRFWSVGPKMTLPLFDGGIRSAEVDRTEAVYDQTVAKYRQTVLDGFREVEN
YLVQLKVYEDEAAVRQEALDAARDSLRLTENQYKAGVIAYLDVVTVQATALSNERTVLNILQSRLIASVQLIAALGGGWD
GQLETTDNR

Sequences:

>Translated_489_residues
MTDRSLINLATVRGSRLLSLSLCVAMLSACAVGPDYQRPQTAEIAQYKEAEGWRQANPSDSLARGAWWELYGDRQLNQLI
EKLNSSNQTVAQSEAQYRQAQALVRSARGAFYPSVDLSAGKTRSSQGTGSSSSSLSSSSSGIRDTYNAQLGVSWEADVWG
KLRRGLEANEASAQASYADLAAMRLSQQSELVQNYLQLRVIDQQKRLLESTVAAYERSLKMTMNQYNAGVSGRDAVAQAQ
TQLKTTQGDLVDLIWQRAQFENAIAVLTGQPPAEFNIAETQDIPKLPQIPLSLPSQLLERRPDIASAERSVIAANANIGV
AKAAYYPDFSLSMSGGYSSSTSQNLISLPNRFWSVGPKMTLPLFDGGIRSAEVDRTEAVYDQTVAKYRQTVLDGFREVEN
YLVQLKVYEDEAAVRQEALDAARDSLRLTENQYKAGVIAYLDVVTVQATALSNERTVLNILQSRLIASVQLIAALGGGWD
GQLETTDNR
>Mature_488_residues
TDRSLINLATVRGSRLLSLSLCVAMLSACAVGPDYQRPQTAEIAQYKEAEGWRQANPSDSLARGAWWELYGDRQLNQLIE
KLNSSNQTVAQSEAQYRQAQALVRSARGAFYPSVDLSAGKTRSSQGTGSSSSSLSSSSSGIRDTYNAQLGVSWEADVWGK
LRRGLEANEASAQASYADLAAMRLSQQSELVQNYLQLRVIDQQKRLLESTVAAYERSLKMTMNQYNAGVSGRDAVAQAQT
QLKTTQGDLVDLIWQRAQFENAIAVLTGQPPAEFNIAETQDIPKLPQIPLSLPSQLLERRPDIASAERSVIAANANIGVA
KAAYYPDFSLSMSGGYSSSTSQNLISLPNRFWSVGPKMTLPLFDGGIRSAEVDRTEAVYDQTVAKYRQTVLDGFREVENY
LVQLKVYEDEAAVRQEALDAARDSLRLTENQYKAGVIAYLDVVTVQATALSNERTVLNILQSRLIASVQLIAALGGGWDG
QLETTDNR

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=465, Percent_Identity=27.741935483871, Blast_Score=183, Evalue=2e-47,
Organism=Escherichia coli, GI2367348, Length=492, Percent_Identity=27.0325203252033, Blast_Score=139, Evalue=3e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 53530; Mature: 53399

Theoretical pI: Translated: 4.99; Mature: 4.99

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCCCC
>Mature Secondary Structure 
TDRSLINLATVRGSRLLSLSLCVAMLSACAVGPDYQRPQTAEIAQYKEAEGWRQANPSD
CCCHHEEHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCCCCCCCC
SLARGAWWELYGDRQLNQLIEKLNSSNQTVAQSEAQYRQAQALVRSARGAFYPSVDLSAG
HHHCCCHHHHHCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
KTRSSQGTGSSSSSLSSSSSGIRDTYNAQLGVSWEADVWGKLRRGLEANEASAQASYADL
CCCCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCHHHHHHHHCCCCCCHHHHHHHHHHH
AAMRLSQQSELVQNYLQLRVIDQQKRLLESTVAAYERSLKMTMNQYNAGVSGRDAVAQAQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHH
TQLKTTQGDLVDLIWQRAQFENAIAVLTGQPPAEFNIAETQDIPKLPQIPLSLPSQLLER
HHHHHCCHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHC
RPDIASAERSVIAANANIGVAKAAYYPDFSLSMSGGYSSSTSQNLISLPNRFWSVGPKMT
CCCCCCCCCCEEEECCCCCEEEHEECCCCEEEECCCCCCCCCHHHHHCCHHHHCCCCCEE
LPLFDGGIRSAEVDRTEAVYDQTVAKYRQTVLDGFREVENYLVQLKVYEDEAAVRQEALD
EEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEEECCHHHHHHHHHH
AARDSLRLTENQYKAGVIAYLDVVTVQATALSNERTVLNILQSRLIASVQLIAALGGGWD
HHHHHHHHCHHHHHCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
GQLETTDNR
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]