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The map label for this gene is algU [H]

Identifier: 77457589

GI number: 77457589

Start: 1536142

End: 1536723

Strand: Direct

Name: algU [H]

Synonym: Pfl01_1362

Alternate gene names: 77457589

Gene position: 1536142-1536723 (Clockwise)

Preceding gene: 77457586

Following gene: 77457590

Centisome position: 23.86

GC content: 55.33

Gene sequence:

>582_bases
ATGCTAACCCAGGAAGAGGATCAGCAGCTGGTCGAACGCGTTCAGCGTGGCGACAAGCGAGCTTTCGATCTGCTGGTGCT
GAAGTATCAGCACAAAATTCTCGGGTTGATCGTGCGTTTTGTGCACGACACCCATGAAGCCCAGGACGTCGCACAAGAAG
CCTTTATCAAGGCGTATCGAGCGCTTGGAAATTTCCGCGGCGACAGTGCGTTTTATACGTGGCTGTACCGCATCGCCATC
AACACGGCGAAGAACTATCTGGTTTCACGCGGCCGTCGGCCGCCGGATAGCGATGTAAGTTCCGAGGATGCAGAGTTCTA
TGACGGCGATCACGGCCTGAAAGATCTCGAGTCACCTGAACGTGCATTGCTGCGGGATGAGATCGAAGGCACCGTCCATC
GAACCATTCAGCAACTGCCAGAGGATTTGCGCACAGCGTTAACTTTGCGCGAATTCGATGGTCTGAGTTACGAGGACATC
GCGAGCGTCATGCAATGTCCGGTTGGTACCGTGCGCTCCCGGATTTTCCGCGCCCGGGAGGCCATCGATAAAGCCCTGCA
GCCGTTGTTGCAGGAAAACTAA

Upstream 100 bases:

>100_bases
TGCTTGGAGGGGGAGAACTTTTGCGAAAAGCCCGAGTCTATGTTTGCAAGTCCGGTCGTTTGGTCATGCAAGCCTCCTCC
GAGTTTATCGAGGAGTGTTC

Downstream 100 bases:

>100_bases
AGACAGCGGCGACAGCCAAGAGAGGAACGCCATGAGTCGTGAAGCCCTGCAGGAATCGCTGTCCGCAGTGATGGATAACG
AAGCGGACGAACTGGAATTG

Product: RNA polymerase sigma factor AlgU

Products: NA

Alternate protein names: Sigma-30 [H]

Number of amino acids: Translated: 193; Mature: 193

Protein sequence:

>193_residues
MLTQEEDQQLVERVQRGDKRAFDLLVLKYQHKILGLIVRFVHDTHEAQDVAQEAFIKAYRALGNFRGDSAFYTWLYRIAI
NTAKNYLVSRGRRPPDSDVSSEDAEFYDGDHGLKDLESPERALLRDEIEGTVHRTIQQLPEDLRTALTLREFDGLSYEDI
ASVMQCPVGTVRSRIFRAREAIDKALQPLLQEN

Sequences:

>Translated_193_residues
MLTQEEDQQLVERVQRGDKRAFDLLVLKYQHKILGLIVRFVHDTHEAQDVAQEAFIKAYRALGNFRGDSAFYTWLYRIAI
NTAKNYLVSRGRRPPDSDVSSEDAEFYDGDHGLKDLESPERALLRDEIEGTVHRTIQQLPEDLRTALTLREFDGLSYEDI
ASVMQCPVGTVRSRIFRAREAIDKALQPLLQEN
>Mature_193_residues
MLTQEEDQQLVERVQRGDKRAFDLLVLKYQHKILGLIVRFVHDTHEAQDVAQEAFIKAYRALGNFRGDSAFYTWLYRIAI
NTAKNYLVSRGRRPPDSDVSSEDAEFYDGDHGLKDLESPERALLRDEIEGTVHRTIQQLPEDLRTALTLREFDGLSYEDI
ASVMQCPVGTVRSRIFRAREAIDKALQPLLQEN

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor regulates genes such as algD, involved in alginate biosynthesis [H]

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=191, Percent_Identity=63.3507853403141, Blast_Score=256, Evalue=8e-70,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR014286
- InterPro:   IPR013325
- InterPro:   IPR013324 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 22251; Mature: 22251

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS01063 SIGMA70_ECF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTQEEDQQLVERVQRGDKRAFDLLVLKYQHKILGLIVRFVHDTHEAQDVAQEAFIKAYR
CCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALGNFRGDSAFYTWLYRIAINTAKNYLVSRGRRPPDSDVSSEDAEFYDGDHGLKDLESPE
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCCCHHHCCCHH
RALLRDEIEGTVHRTIQQLPEDLRTALTLREFDGLSYEDIASVMQCPVGTVRSRIFRARE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHH
AIDKALQPLLQEN
HHHHHHHHHHCCC
>Mature Secondary Structure
MLTQEEDQQLVERVQRGDKRAFDLLVLKYQHKILGLIVRFVHDTHEAQDVAQEAFIKAYR
CCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALGNFRGDSAFYTWLYRIAINTAKNYLVSRGRRPPDSDVSSEDAEFYDGDHGLKDLESPE
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHCCCCCHHHCCCHH
RALLRDEIEGTVHRTIQQLPEDLRTALTLREFDGLSYEDIASVMQCPVGTVRSRIFRARE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHH
AIDKALQPLLQEN
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8432708; 8378309; 7961421; 10984043; 7737518 [H]