Click here to switch to the map view.

The map label for this gene is pfpI [H]

Identifier: 77457530

GI number: 77457530

Start: 1479779

End: 1480360

Strand: Direct

Name: pfpI [H]

Synonym: Pfl01_1303

Alternate gene names: 77457530

Gene position: 1479779-1480360 (Clockwise)

Preceding gene: 77457528

Following gene: 77457531

Centisome position: 22.98

GC content: 62.89

Gene sequence:

>582_bases
ATGGCCGCGAAAAAAATTCTGATGCTGGTCGGCGATTACGTCGAAGACTACGAAGTGATGGTGCCGTTCCAGGCCCTGCA
AATGGTCGGTCACACCGTGCATGCCGTGTGCCCGGACAAGGCCGCCGGCAAGACCGTGCGCACCGCGATCCATGACTTTG
AAGGCGATCAGACCTACAGCGAGAAACCCGGTCACCAGTTCGCCCTGAACTTCGATTTCGCCAAGGTCGATGCCAAGGAT
TACGACGCCTTGCTGGTGCCCGGTGGGCGTGCGCCGGAATACCTGCGGCTGAACGAAAAAGTCCTGGAACTGGTGCGCGC
CTTCGACAAGGCCGGCAAGCCGATTGCGGCCGTCTGTCATGGCGCGCAACTGCTGGCGGCGGCGGGGATTCTCGAAGGTC
GCGAGTGCAGTGCTTATCCGGCCTGTGCCCCGGAAGTGCGCTTGGCCGGCGGCACCTACATCGACATCCCGGTCACGGAC
GGCCATGTCCAGGGCAACCTGGCCACCGCGCCCGCCTGGCCGGCGCACCCGAAATGGCTGGCCGGTTTCCTCGGTTTGCT
TGGAACCAAAATCACCCTGTGA

Upstream 100 bases:

>100_bases
TTACAGCTGAGCGCAGAGTTATCCATACAGTTTGCGAACGCCATCGCGGTCCTTGGGCTACTGTGAATATTCCTGCGTAT
TCACCCGAGGAGCCCTGCCC

Downstream 100 bases:

>100_bases
CACATCGATAAGGAGAAACACCATGTCCGGATGGTATGAGTTGAGCAAAAGCAGCAACGGCCAGTACAAGTTCGTGCTGA
AAGCGGCGAATGCCGAAACC

Product: peptidase C56, PfpI

Products: NA

Alternate protein names: Intracellular protease I [H]

Number of amino acids: Translated: 193; Mature: 192

Protein sequence:

>193_residues
MAAKKILMLVGDYVEDYEVMVPFQALQMVGHTVHAVCPDKAAGKTVRTAIHDFEGDQTYSEKPGHQFALNFDFAKVDAKD
YDALLVPGGRAPEYLRLNEKVLELVRAFDKAGKPIAAVCHGAQLLAAAGILEGRECSAYPACAPEVRLAGGTYIDIPVTD
GHVQGNLATAPAWPAHPKWLAGFLGLLGTKITL

Sequences:

>Translated_193_residues
MAAKKILMLVGDYVEDYEVMVPFQALQMVGHTVHAVCPDKAAGKTVRTAIHDFEGDQTYSEKPGHQFALNFDFAKVDAKD
YDALLVPGGRAPEYLRLNEKVLELVRAFDKAGKPIAAVCHGAQLLAAAGILEGRECSAYPACAPEVRLAGGTYIDIPVTD
GHVQGNLATAPAWPAHPKWLAGFLGLLGTKITL
>Mature_192_residues
AAKKILMLVGDYVEDYEVMVPFQALQMVGHTVHAVCPDKAAGKTVRTAIHDFEGDQTYSEKPGHQFALNFDFAKVDAKDY
DALLVPGGRAPEYLRLNEKVLELVRAFDKAGKPIAAVCHGAQLLAAAGILEGRECSAYPACAPEVRLAGGTYIDIPVTDG
HVQGNLATAPAWPAHPKWLAGFLGLLGTKITL

Specific function: Highly stable protease. Although the physiological function is unclear it is the dominant protease in P.furiosus [H]

COG id: COG0693

COG function: function code R; Putative intracellular protease/amidase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PfpI endopeptidase domain [H]

Homologues:

Organism=Escherichia coli, GI87082219, Length=186, Percent_Identity=32.258064516129, Blast_Score=92, Evalue=2e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006286
- InterPro:   IPR002818 [H]

Pfam domain/function: PF01965 DJ-1_PfpI [H]

EC number: NA

Molecular weight: Translated: 20698; Mature: 20567

Theoretical pI: Translated: 6.39; Mature: 6.39

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAKKILMLVGDYVEDYEVMVPFQALQMVGHTVHAVCPDKAAGKTVRTAIHDFEGDQTYS
CCHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHEEECCCCCCCHHHHHHHHHCCCCCCCC
EKPGHQFALNFDFAKVDAKDYDALLVPGGRAPEYLRLNEKVLELVRAFDKAGKPIAAVCH
CCCCCEEEEECCEEEECCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHH
GAQLLAAAGILEGRECSAYPACAPEVRLAGGTYIDIPVTDGHVQGNLATAPAWPAHPKWL
HHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCEEEEECCCCEECCCEECCCCCCCCHHHH
AGFLGLLGTKITL
HHHHHHHHHCCCC
>Mature Secondary Structure 
AAKKILMLVGDYVEDYEVMVPFQALQMVGHTVHAVCPDKAAGKTVRTAIHDFEGDQTYS
CHHHHHHHHHHHHCCCEEEECHHHHHHHHHHHEEECCCCCCCHHHHHHHHHCCCCCCCC
EKPGHQFALNFDFAKVDAKDYDALLVPGGRAPEYLRLNEKVLELVRAFDKAGKPIAAVCH
CCCCCEEEEECCEEEECCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHH
GAQLLAAAGILEGRECSAYPACAPEVRLAGGTYIDIPVTDGHVQGNLATAPAWPAHPKWL
HHHHHHHHHCCCCCCCCCCCCCCCCEEECCCCEEEEECCCCEECCCEECCCCCCCCHHHH
AGFLGLLGTKITL
HHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8626329 [H]