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The map label for this gene is cls [H]

Identifier: 77457523

GI number: 77457523

Start: 1471501

End: 1472658

Strand: Direct

Name: cls [H]

Synonym: Pfl01_1296

Alternate gene names: 77457523

Gene position: 1471501-1472658 (Clockwise)

Preceding gene: 77457522

Following gene: 77457525

Centisome position: 22.86

GC content: 64.34

Gene sequence:

>1158_bases
ATGCGCGGCGCGGTGTTTCCGTGGCGTGATGGCAACCGCTTCGAGCTGTTGATCGACGGCCCGCAATTCTTCCCGCGCAT
GCTCGACGAGATTGCCCGCGCCCGTGAGCAGATCGAACTGGAGTTGTATCTGGTGGAAGCCGGTGCCTGTGCCGAAACCA
TCGTGCAGGCACTGGTGCTGGCGGCGGAGCGGGGCGTGCGGGTGCGTTGCCTGTTCGATGATTACGGCAGCCTCGCGTTC
ACCCTCACGCTGCGCCGGCGGCTGACTGGTGCCGGGGTCGAGCTGCGTTTTTACAACCGGCTGAACTGGCGGCGCTGGGT
CGGCAATTTCTATCGCGATCATCGCAAGCTGTTGCTGGTCGACCAGCGTCTGGCGGTGGTCGGCGGCACCGGGGTCACCG
ATGAGTTCTGGACGCCGGGCCACGACACCAGCGAATGGCACGAGGTCATGGTGGAAATCACCGGCCCGCTGGTGCTCGAC
TGGCAGTTGCTGTTCGACCGCCAATGGATCGCCAACCGCCATCGCCGGGCCTGGCGGCCCAACGCGCATTTTGGGCTGCC
ACGGTTGCCCCGTGTGCCGGACACGGGCGAGGGCATGGGCCGTGTGGCCTACGCCGACGCCCGCCAGCATCGGGACATTC
TGCAATCGCTGTTCCGCGCCTTGAACAGTGGCCAAAAGCGTATCTGGCTGGCCACGCCGTACTTCCTGCCGACCTGGAAA
ATCCGCCGCTCCCTGCGCAAGGCTGCCGCCCGTGGCCTCGACGTGCGTCTGCTGCTGACCGGGCCGCGCACCGATCACCC
GTCGGTGCGTTATGCCGGGCATCGCTACTACCCGCGCCTGCTCAAGGCCGGGGTGAAAATCTACGAATACCAGCCGTGTT
TCCTGCACCTGAAAATGGTGCTGGTGGACGACTGGGTGAGCATCGGCTCGTGCAATTTCGATCACTGGAATCTGCGCTTC
AATCTTGAAGCGAATCTGGAAGCGCTGGATCCGTCATTGACGGCAGCGGTGGCGGCGAGTTTCGAGAAGGACTTCGGCCT
GAGTCAGCAGGTGAGTCTGGAGGAATGGCGAAAACGGCCACTGTGGCGGCGGGTGAAGCAGCGGATCTGGGGCTGGGTGG
ATCGGGTGGTGGTCAACTTGCTCGACAGACGCGGCTAG

Upstream 100 bases:

>100_bases
CTGCTGCCGGGCCTGGAAAGCCTGCGCAACCTGGCCGGCCTGTCGGCCATCAGTCTGTCGGTGCCGGTGGGTGCGGTGCT
GATCTTCACGGCGCGCTGAC

Downstream 100 bases:

>100_bases
TCGCTGTCCCCGATTGATCGTTCCCACGTCGAGGCGTCGAACCGTCCGCGTGGGAATGCAGCCCGGGACGCGCCGCGTTC
CAAAGCGGACGCAGAGCGTC

Product: phospholipase D/transphosphatidylase

Products: cardiolipin; glycerol [C]

Alternate protein names: CL synthase [H]

Number of amino acids: Translated: 385; Mature: 385

Protein sequence:

>385_residues
MRGAVFPWRDGNRFELLIDGPQFFPRMLDEIARAREQIELELYLVEAGACAETIVQALVLAAERGVRVRCLFDDYGSLAF
TLTLRRRLTGAGVELRFYNRLNWRRWVGNFYRDHRKLLLVDQRLAVVGGTGVTDEFWTPGHDTSEWHEVMVEITGPLVLD
WQLLFDRQWIANRHRRAWRPNAHFGLPRLPRVPDTGEGMGRVAYADARQHRDILQSLFRALNSGQKRIWLATPYFLPTWK
IRRSLRKAAARGLDVRLLLTGPRTDHPSVRYAGHRYYPRLLKAGVKIYEYQPCFLHLKMVLVDDWVSIGSCNFDHWNLRF
NLEANLEALDPSLTAAVAASFEKDFGLSQQVSLEEWRKRPLWRRVKQRIWGWVDRVVVNLLDRRG

Sequences:

>Translated_385_residues
MRGAVFPWRDGNRFELLIDGPQFFPRMLDEIARAREQIELELYLVEAGACAETIVQALVLAAERGVRVRCLFDDYGSLAF
TLTLRRRLTGAGVELRFYNRLNWRRWVGNFYRDHRKLLLVDQRLAVVGGTGVTDEFWTPGHDTSEWHEVMVEITGPLVLD
WQLLFDRQWIANRHRRAWRPNAHFGLPRLPRVPDTGEGMGRVAYADARQHRDILQSLFRALNSGQKRIWLATPYFLPTWK
IRRSLRKAAARGLDVRLLLTGPRTDHPSVRYAGHRYYPRLLKAGVKIYEYQPCFLHLKMVLVDDWVSIGSCNFDHWNLRF
NLEANLEALDPSLTAAVAASFEKDFGLSQQVSLEEWRKRPLWRRVKQRIWGWVDRVVVNLLDRRG
>Mature_385_residues
MRGAVFPWRDGNRFELLIDGPQFFPRMLDEIARAREQIELELYLVEAGACAETIVQALVLAAERGVRVRCLFDDYGSLAF
TLTLRRRLTGAGVELRFYNRLNWRRWVGNFYRDHRKLLLVDQRLAVVGGTGVTDEFWTPGHDTSEWHEVMVEITGPLVLD
WQLLFDRQWIANRHRRAWRPNAHFGLPRLPRVPDTGEGMGRVAYADARQHRDILQSLFRALNSGQKRIWLATPYFLPTWK
IRRSLRKAAARGLDVRLLLTGPRTDHPSVRYAGHRYYPRLLKAGVKIYEYQPCFLHLKMVLVDDWVSIGSCNFDHWNLRF
NLEANLEALDPSLTAAVAASFEKDFGLSQQVSLEEWRKRPLWRRVKQRIWGWVDRVVVNLLDRRG

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787007, Length=374, Percent_Identity=32.3529411764706, Blast_Score=158, Evalue=5e-40,
Organism=Escherichia coli, GI1787502, Length=366, Percent_Identity=28.6885245901639, Blast_Score=157, Evalue=1e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.- [C]

Molecular weight: Translated: 45027; Mature: 45027

Theoretical pI: Translated: 10.44; Mature: 10.44

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGAVFPWRDGNRFELLIDGPQFFPRMLDEIARAREQIELELYLVEAGACAETIVQALVL
CCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHEEEEEEEEECCCHHHHHHHHHHH
AAERGVRVRCLFDDYGSLAFTLTLRRRLTGAGVELRFYNRLNWRRWVGNFYRDHRKLLLV
HHCCCCEEEEEECCCCCEEEEEEEHHHHCCCCEEEEEECCCCHHHHHHHHHHCCCEEEEE
DQRLAVVGGTGVTDEFWTPGHDTSEWHEVMVEITGPLVLDWQLLFDRQWIANRHRRAWRP
ECEEEEECCCCCCCCCCCCCCCCHHHHHHHHEECCCEEEEHHHHHHHHHHHHHHHHCCCC
NAHFGLPRLPRVPDTGEGMGRVAYADARQHRDILQSLFRALNSGQKRIWLATPYFLPTWK
CCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHH
IRRSLRKAAARGLDVRLLLTGPRTDHPSVRYAGHRYYPRLLKAGVKIYEYQPCFLHLKMV
HHHHHHHHHHCCCEEEEEEECCCCCCCCCEECCCHHHHHHHHCCCEEEEECCHHHHHHHH
LVDDWVSIGSCNFDHWNLRFNLEANLEALDPSLTAAVAASFEKDFGLSQQVSLEEWRKRP
HHHHHHHHCCCCCCEEEEEEEEECCCHHCCCHHHHHHHHHHHHHCCCCCCCCHHHHHCCH
LWRRVKQRIWGWVDRVVVNLLDRRG
HHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MRGAVFPWRDGNRFELLIDGPQFFPRMLDEIARAREQIELELYLVEAGACAETIVQALVL
CCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHEEEEEEEEECCCHHHHHHHHHHH
AAERGVRVRCLFDDYGSLAFTLTLRRRLTGAGVELRFYNRLNWRRWVGNFYRDHRKLLLV
HHCCCCEEEEEECCCCCEEEEEEEHHHHCCCCEEEEEECCCCHHHHHHHHHHCCCEEEEE
DQRLAVVGGTGVTDEFWTPGHDTSEWHEVMVEITGPLVLDWQLLFDRQWIANRHRRAWRP
ECEEEEECCCCCCCCCCCCCCCCHHHHHHHHEECCCEEEEHHHHHHHHHHHHHHHHCCCC
NAHFGLPRLPRVPDTGEGMGRVAYADARQHRDILQSLFRALNSGQKRIWLATPYFLPTWK
CCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHH
IRRSLRKAAARGLDVRLLLTGPRTDHPSVRYAGHRYYPRLLKAGVKIYEYQPCFLHLKMV
HHHHHHHHHHCCCEEEEEEECCCCCCCCCEECCCHHHHHHHHCCCEEEEECCHHHHHHHH
LVDDWVSIGSCNFDHWNLRFNLEANLEALDPSLTAAVAASFEKDFGLSQQVSLEEWRKRP
HHHHHHHHCCCCCCEEEEEEEEECCCHHCCCHHHHHHHHHHHHHCCCCCCCCHHHHHCCH
LWRRVKQRIWGWVDRVVVNLLDRRG
HHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 2 L-1-phosphatidyl-glycerol [C]

Specific reaction: 2 L-1-phosphatidyl-glycerol = cardiolipin + glycerol [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10585141 [H]