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The map label for this gene is rpoS [H]

Identifier: 77457359

GI number: 77457359

Start: 1308419

End: 1309426

Strand: Direct

Name: rpoS [H]

Synonym: Pfl01_1132

Alternate gene names: 77457359

Gene position: 1308419-1309426 (Clockwise)

Preceding gene: 77457358

Following gene: 77457362

Centisome position: 20.32

GC content: 57.44

Gene sequence:

>1008_bases
ATGGCTCTCAGTAAAGAAGTGCCGGAGTTTGACATCGACGATGAGGTTCTCCTGATGGAGACCGGCATCGATTCGGATTC
GATGTCGAATGATGAAGGGGCTGCTCCACCTTCCGTTCGTGCCAAATCCAAACACTCCGCTTCACTTAAACAACATAAGT
ACATCGATTACACTCGGGCACTCGATGCCACGCAGCTGTACCTCAACGAAATCGGCTTTTCCCCACTGCTCTCCCCGGAA
GAAGAAGTTCATTTTGCGCGCTTGTCGCAAAGTGGCGATCCTGCCGGGCGCAAGCGCATGATCGAAAGTAACCTGCGGCT
GGTGGTCAAGATCGCCCGGCGTTACGTCAATCGGGGGCTGTCGCTGCTGGATCTGATCGAAGAGGGCAACCTCGGACTGA
TCCGAGCGGTGGAAAAGTTCGATCCTGAGCGCGGCTTCCGCTTCTCGACCTACGCAACCTGGTGGATTCGTCAGACCATC
GAACGCGCGATCATGAATCAGACCCGGACCATCCGGCTGCCGATTCATGTGGTCAAGGAACTCAACGTGTACCTGCGGGC
TGCACGGGAGCTGACGCAAAAGCTCGACCACGAACCTTCACCCGAAGAAATCGCCAACCTGCTGGAAAAACCGGTGGGCG
AGGTCAAGCGCATGCTGGGCCTGAACGAGCGGGTTTCTTCGGTCGACGTCTCGCTGGGTCCGGATTCGGATAAAACCCTG
CTGGACACCCTGACGGATGACCGTCCGACTGATCCGTGCGAACTGCTGCAGGATGACGATCTGTCGCAGAGCATCGATCA
ATGGCTCTCGGAGCTGACCGACAAGCAACGCGAGGTGGTGGTACGCCGCTTCGGTCTGCGCGGCCACGAGAGCAGCACGC
TGGAAGATGTAGGCCTGGAGATCGGCCTGACCCGGGAACGGGTGAGACAGATCCAGGTGGAAGGCCTTAAGCGCCTTCGG
GAAATTCTCGAGAAAAACGGCCTGTCGAGCGAGTCGCTGTTTCAATAA

Upstream 100 bases:

>100_bases
GACCAGCCTGTTCCGTCACGTAGAGGGGACAGGCTCCAGCGTTGCCAAGGATAAAGGCGCCGCTTGAGCTTGGGGTCGAA
CTCACCAAAGGACTATAACA

Downstream 100 bases:

>100_bases
GCCGCCCGCCTGACCGCCATGCAGAAAGCCCCGACTGGTTCGGGGCTTTTTGTTTGTGTGCTGAAAGCGGCAGGTATTCC
CACCTTGTAGGTTTGCGCTG

Product: RNA polymerase sigma factor RpoS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 335; Mature: 334

Protein sequence:

>335_residues
MALSKEVPEFDIDDEVLLMETGIDSDSMSNDEGAAPPSVRAKSKHSASLKQHKYIDYTRALDATQLYLNEIGFSPLLSPE
EEVHFARLSQSGDPAGRKRMIESNLRLVVKIARRYVNRGLSLLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTI
ERAIMNQTRTIRLPIHVVKELNVYLRAARELTQKLDHEPSPEEIANLLEKPVGEVKRMLGLNERVSSVDVSLGPDSDKTL
LDTLTDDRPTDPCELLQDDDLSQSIDQWLSELTDKQREVVVRRFGLRGHESSTLEDVGLEIGLTRERVRQIQVEGLKRLR
EILEKNGLSSESLFQ

Sequences:

>Translated_335_residues
MALSKEVPEFDIDDEVLLMETGIDSDSMSNDEGAAPPSVRAKSKHSASLKQHKYIDYTRALDATQLYLNEIGFSPLLSPE
EEVHFARLSQSGDPAGRKRMIESNLRLVVKIARRYVNRGLSLLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTI
ERAIMNQTRTIRLPIHVVKELNVYLRAARELTQKLDHEPSPEEIANLLEKPVGEVKRMLGLNERVSSVDVSLGPDSDKTL
LDTLTDDRPTDPCELLQDDDLSQSIDQWLSELTDKQREVVVRRFGLRGHESSTLEDVGLEIGLTRERVRQIQVEGLKRLR
EILEKNGLSSESLFQ
>Mature_334_residues
ALSKEVPEFDIDDEVLLMETGIDSDSMSNDEGAAPPSVRAKSKHSASLKQHKYIDYTRALDATQLYLNEIGFSPLLSPEE
EVHFARLSQSGDPAGRKRMIESNLRLVVKIARRYVNRGLSLLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIE
RAIMNQTRTIRLPIHVVKELNVYLRAARELTQKLDHEPSPEEIANLLEKPVGEVKRMLGLNERVSSVDVSLGPDSDKTLL
DTLTDDRPTDPCELLQDDDLSQSIDQWLSELTDKQREVVVRRFGLRGHESSTLEDVGLEIGLTRERVRQIQVEGLKRLRE
ILEKNGLSSESLFQ

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is involved in acid resistance [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789098, Length=277, Percent_Identity=75.812274368231, Blast_Score=423, Evalue=1e-120,
Organism=Escherichia coli, GI1789448, Length=225, Percent_Identity=41.7777777777778, Blast_Score=199, Evalue=2e-52,
Organism=Escherichia coli, GI1789871, Length=280, Percent_Identity=31.0714285714286, Blast_Score=100, Evalue=2e-22,

Paralogues:

None

Copy number: <10 (log phase) 250 (stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012761
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 38147; Mature: 38016

Theoretical pI: Translated: 4.89; Mature: 4.89

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALSKEVPEFDIDDEVLLMETGIDSDSMSNDEGAAPPSVRAKSKHSASLKQHKYIDYTRA
CCCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
LDATQLYLNEIGFSPLLSPEEEVHFARLSQSGDPAGRKRMIESNLRLVVKIARRYVNRGL
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
SLLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMNQTRTIRLPIHVVKE
HHHHHHHCCCCHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHH
LNVYLRAARELTQKLDHEPSPEEIANLLEKPVGEVKRMLGLNERVSSVDVSLGPDSDKTL
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHEEEECCCCCCHHH
LDTLTDDRPTDPCELLQDDDLSQSIDQWLSELTDKQREVVVRRFGLRGHESSTLEDVGLE
HHHHCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCC
IGLTRERVRQIQVEGLKRLREILEKNGLSSESLFQ
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCC
>Mature Secondary Structure 
ALSKEVPEFDIDDEVLLMETGIDSDSMSNDEGAAPPSVRAKSKHSASLKQHKYIDYTRA
CCCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
LDATQLYLNEIGFSPLLSPEEEVHFARLSQSGDPAGRKRMIESNLRLVVKIARRYVNRGL
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
SLLDLIEEGNLGLIRAVEKFDPERGFRFSTYATWWIRQTIERAIMNQTRTIRLPIHVVKE
HHHHHHHCCCCHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHH
LNVYLRAARELTQKLDHEPSPEEIANLLEKPVGEVKRMLGLNERVSSVDVSLGPDSDKTL
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHEEEECCCCCCHHH
LDTLTDDRPTDPCELLQDDDLSQSIDQWLSELTDKQREVVVRRFGLRGHESSTLEDVGLE
HHHHCCCCCCCHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHCCC
IGLTRERVRQIQVEGLKRLREILEKNGLSSESLFQ
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7959068; 10984043 [H]