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The map label for this gene is ispD [H]

Identifier: 77457350

GI number: 77457350

Start: 1300502

End: 1301209

Strand: Direct

Name: ispD [H]

Synonym: Pfl01_1123

Alternate gene names: 77457350

Gene position: 1300502-1301209 (Clockwise)

Preceding gene: 77457349

Following gene: 77457352

Centisome position: 20.2

GC content: 62.99

Gene sequence:

>708_bases
ATGATCGATTCCCTGCCGGCCTTCTGGGCCGTGATTCCTGCCGCGGGCGTCGGTGCCCGAATGGCCGCGGACCGTCCCAA
GCAATACCTGCAACTGGGCGGGCGCACAATTCTCGAACACAGCCTCGGCTGTTTCCTCGATCACCCGAGCCTCAAGGGCC
TGGTGGTCAGTCTTGCCGTCGATGATCCTTATTGGCCGAACCTGGCCAGCGCCAGTGATCCGCGAATTCAACGGGTCGAC
GGTGGCACCGAACGTTCCGGCTCGGTACTCAATGCCTTGCTGCATCTGCACGCCCAAGGTGCCGACGACGAGGACTGGGT
GCTGGTGCACGATGCGGCGCGGCCCAATCTGAGCCGTGACGATCTCGACAAATTGCTCGCTGAGCTGGCGAACGATCCGG
TCGGTGGTTTGCTGGCTGTGCCGGCGAAGGACACTCTCAAGCGGGTCGACAAGCACGGTCGGGTGGTGGAAACCGTCGAT
CGCAGTGTGATCTGGCAAGCCTATACGCCGCAGATGTTCCGCCTCGGTGCGCTGCACCGGGCTTTGGCCGACAGTCTCGT
GGCGGTTGCAGTCATCACCGATGAAGCTTCGGCGATGGAGTGGGCTGGCATGGCGCCGCGTCTGATCGAGGGCCGCGCGG
ATAACTTGAAAGTCACCCGCCCCGAAGACCTCGAATGGTTGCGTCAGCGCTGGACCAACCGTCGTTAA

Upstream 100 bases:

>100_bases
AAGTCAGCGAGTTGAAGAAAGGCATGGAGACCGTTGAAGAGCGGGCTCGTCACGAATTGGGCATGGTCAAGGACGGCGAA
ACCCTTTACCAGTTGGCCCA

Downstream 100 bases:

>100_bases
GCTCGATATTCCGGCCGTTCGGCCAAACCTTCCTTGAGAAAATCCACCAGTTTGCGCACCTTGGGCGACAGGTGCCGCTG
CTGCGGATACAGCGCCCACA

Product: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Products: NA

Alternate protein names: 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT [H]

Number of amino acids: Translated: 235; Mature: 235

Protein sequence:

>235_residues
MIDSLPAFWAVIPAAGVGARMAADRPKQYLQLGGRTILEHSLGCFLDHPSLKGLVVSLAVDDPYWPNLASASDPRIQRVD
GGTERSGSVLNALLHLHAQGADDEDWVLVHDAARPNLSRDDLDKLLAELANDPVGGLLAVPAKDTLKRVDKHGRVVETVD
RSVIWQAYTPQMFRLGALHRALADSLVAVAVITDEASAMEWAGMAPRLIEGRADNLKVTRPEDLEWLRQRWTNRR

Sequences:

>Translated_235_residues
MIDSLPAFWAVIPAAGVGARMAADRPKQYLQLGGRTILEHSLGCFLDHPSLKGLVVSLAVDDPYWPNLASASDPRIQRVD
GGTERSGSVLNALLHLHAQGADDEDWVLVHDAARPNLSRDDLDKLLAELANDPVGGLLAVPAKDTLKRVDKHGRVVETVD
RSVIWQAYTPQMFRLGALHRALADSLVAVAVITDEASAMEWAGMAPRLIEGRADNLKVTRPEDLEWLRQRWTNRR
>Mature_235_residues
MIDSLPAFWAVIPAAGVGARMAADRPKQYLQLGGRTILEHSLGCFLDHPSLKGLVVSLAVDDPYWPNLASASDPRIQRVD
GGTERSGSVLNALLHLHAQGADDEDWVLVHDAARPNLSRDDLDKLLAELANDPVGGLLAVPAKDTLKRVDKHGRVVETVD
RSVIWQAYTPQMFRLGALHRALADSLVAVAVITDEASAMEWAGMAPRLIEGRADNLKVTRPEDLEWLRQRWTNRR

Specific function: Catalyzes the formation of 4-diphosphocytidyl-2-C- methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4- phosphate (MEP) [H]

COG id: COG1211

COG function: function code I; 4-diphosphocytidyl-2-methyl-D-erithritol synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispD family [H]

Homologues:

Organism=Escherichia coli, GI1789104, Length=217, Percent_Identity=50.2304147465438, Blast_Score=203, Evalue=7e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001228
- InterPro:   IPR018294 [H]

Pfam domain/function: PF01128 IspD [H]

EC number: =2.7.7.60 [H]

Molecular weight: Translated: 25842; Mature: 25842

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS01295 ISPD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIDSLPAFWAVIPAAGVGARMAADRPKQYLQLGGRTILEHSLGCFLDHPSLKGLVVSLAV
CCCCHHHHHHHHHCCCCCCHHHHCCHHHHHHHCCHHHHHHHHHHEECCCCCCEEEEEEEE
DDPYWPNLASASDPRIQRVDGGTERSGSVLNALLHLHAQGADDEDWVLVHDAARPNLSRD
CCCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCHH
DLDKLLAELANDPVGGLLAVPAKDTLKRVDKHGRVVETVDRSVIWQAYTPQMFRLGALHR
HHHHHHHHHHCCCCCCEEEECCHHHHHHHHHCCCHHHHHHHHHEEHHCCHHHHHHHHHHH
ALADSLVAVAVITDEASAMEWAGMAPRLIEGRADNLKVTRPEDLEWLRQRWTNRR
HHHHHHEEEEEEECCCHHHHHCCCCCHHHCCCCCCEEEECCHHHHHHHHHHCCCC
>Mature Secondary Structure
MIDSLPAFWAVIPAAGVGARMAADRPKQYLQLGGRTILEHSLGCFLDHPSLKGLVVSLAV
CCCCHHHHHHHHHCCCCCCHHHHCCHHHHHHHCCHHHHHHHHHHEECCCCCCEEEEEEEE
DDPYWPNLASASDPRIQRVDGGTERSGSVLNALLHLHAQGADDEDWVLVHDAARPNLSRD
CCCCCCCCCCCCCCCEEECCCCCCCHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCHH
DLDKLLAELANDPVGGLLAVPAKDTLKRVDKHGRVVETVDRSVIWQAYTPQMFRLGALHR
HHHHHHHHHHCCCCCCEEEECCHHHHHHHHHCCCHHHHHHHHHEEHHCCHHHHHHHHHHH
ALADSLVAVAVITDEASAMEWAGMAPRLIEGRADNLKVTRPEDLEWLRQRWTNRR
HHHHHHEEEEEEECCCHHHHHCCCCCHHHCCCCCCEEEECCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA