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The map label for this gene is rnhB

Identifier: 77457342

GI number: 77457342

Start: 1289051

End: 1289692

Strand: Direct

Name: rnhB

Synonym: Pfl01_1115

Alternate gene names: 77457342

Gene position: 1289051-1289692 (Clockwise)

Preceding gene: 77457341

Following gene: 77457343

Centisome position: 20.02

GC content: 61.68

Gene sequence:

>642_bases
ATGAGCAAGACAAGCATGCAGATGGGGCTGGATTTTACCCTGGTCGCCGAAGTCGAAGAACTGGTGGCCGGTGTCGATGA
AGTCGGTCGTGGCCCGCTGTGCGGCGCGGTCGTCACGGCCGCGGTTATCCTCGATCCGAACCGCCCGATTCTCGGCCTCA
ACGACTCGAAGAAACTCACCGAAGCCAAGCGCGAAAAGCTCTACGATGAAATCTGCGAGAAGGCGCTGAGCTGGTGCATC
GCCCGCGCCGAGGTCGAAGAAATCGACGAACTGAACATCCTTCACGCCACCATGCTGGCGATGCAGCGCGCTGTCGCTGG
CCTGCACATCCAGCCGAAACTGGCGATGATCGACGGCAACCGTTGCCCGAAACTGCCGATGCGTTCCGAAGCGGTGGTCA
AGGGCGACAGCAAGGTTCCGGCCATCGCCGCGGCCTCGATTCTGGCCAAGGTCAGCCGCGACCGCGAGATGGCGGCGTTC
GAATTGATCTACCCGGGTTACGGCATCAGTGGTCACAAAGGCTACCCGACGCCCGTTCATCTGGAAGCGCTGGTGCGCCT
GGGGCCGACGCCGATTCACCGGCGTTCTTTTGCCCCGGTTCGTCAGGCTTATGAAGCGCTGGAAGGTCTCGTGCAGGTTT
AG

Upstream 100 bases:

>100_bases
AAGAGCAGACCCGCGGCTTCGACGAGATCCATCGCACCCTGCGCCTGGACGCTTCCAATCAGGCGGCTGATGCCGTTCTC
AACCTGATCGGGCAAACACG

Downstream 100 bases:

>100_bases
CGGCGAGGCTGATGTTTTGTTCGGGGCCCGGTACAATCCGGGCCTTGTTGTCTCTATGTAACTGGACAGGATCACTATGC
CGGCTTCATTCGTTCATCTA

Product: ribonuclease HII

Products: NA

Alternate protein names: RNase HII

Number of amino acids: Translated: 213; Mature: 212

Protein sequence:

>213_residues
MSKTSMQMGLDFTLVAEVEELVAGVDEVGRGPLCGAVVTAAVILDPNRPILGLNDSKKLTEAKREKLYDEICEKALSWCI
ARAEVEEIDELNILHATMLAMQRAVAGLHIQPKLAMIDGNRCPKLPMRSEAVVKGDSKVPAIAAASILAKVSRDREMAAF
ELIYPGYGISGHKGYPTPVHLEALVRLGPTPIHRRSFAPVRQAYEALEGLVQV

Sequences:

>Translated_213_residues
MSKTSMQMGLDFTLVAEVEELVAGVDEVGRGPLCGAVVTAAVILDPNRPILGLNDSKKLTEAKREKLYDEICEKALSWCI
ARAEVEEIDELNILHATMLAMQRAVAGLHIQPKLAMIDGNRCPKLPMRSEAVVKGDSKVPAIAAASILAKVSRDREMAAF
ELIYPGYGISGHKGYPTPVHLEALVRLGPTPIHRRSFAPVRQAYEALEGLVQV
>Mature_212_residues
SKTSMQMGLDFTLVAEVEELVAGVDEVGRGPLCGAVVTAAVILDPNRPILGLNDSKKLTEAKREKLYDEICEKALSWCIA
RAEVEEIDELNILHATMLAMQRAVAGLHIQPKLAMIDGNRCPKLPMRSEAVVKGDSKVPAIAAASILAKVSRDREMAAFE
LIYPGYGISGHKGYPTPVHLEALVRLGPTPIHRRSFAPVRQAYEALEGLVQV

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0164

COG function: function code L; Ribonuclease HII

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNase HII family

Homologues:

Organism=Escherichia coli, GI1786380, Length=184, Percent_Identity=72.8260869565217, Blast_Score=273, Evalue=5e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH2_PSEPF (Q3KH98)

Other databases:

- EMBL:   CP000094
- RefSeq:   YP_346847.1
- ProteinModelPortal:   Q3KH98
- SMR:   Q3KH98
- STRING:   Q3KH98
- GeneID:   3716539
- GenomeReviews:   CP000094_GR
- KEGG:   pfo:Pfl01_1115
- eggNOG:   COG0164
- HOGENOM:   HBG584843
- OMA:   RLGPTPI
- ProtClustDB:   PRK00015
- BioCyc:   PFLU205922:PFL_1115-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00052_B
- InterPro:   IPR022898
- InterPro:   IPR001352
- InterPro:   IPR012337
- PANTHER:   PTHR10954

Pfam domain/function: PF01351 RNase_HII; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 23137; Mature: 23006

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKTSMQMGLDFTLVAEVEELVAGVDEVGRGPLCGAVVTAAVILDPNRPILGLNDSKKLT
CCCCCHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHEECCCCCEECCCCCHHHH
EAKREKLYDEICEKALSWCIARAEVEEIDELNILHATMLAMQRAVAGLHIQPKLAMIDGN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCC
RCPKLPMRSEAVVKGDSKVPAIAAASILAKVSRDREMAAFELIYPGYGISGHKGYPTPVH
CCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHCCCHHHEEEEEECCCCCCCCCCCCCCHH
LEALVRLGPTPIHRRSFAPVRQAYEALEGLVQV
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SKTSMQMGLDFTLVAEVEELVAGVDEVGRGPLCGAVVTAAVILDPNRPILGLNDSKKLT
CCCCHHHCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHEECCCCCEECCCCCHHHH
EAKREKLYDEICEKALSWCIARAEVEEIDELNILHATMLAMQRAVAGLHIQPKLAMIDGN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCC
RCPKLPMRSEAVVKGDSKVPAIAAASILAKVSRDREMAAFELIYPGYGISGHKGYPTPVH
CCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHCCCHHHEEEEEECCCCCCCCCCCCCCHH
LEALVRLGPTPIHRRSFAPVRQAYEALEGLVQV
HHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA