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The map label for this gene is tsf [H]

Identifier: 77457329

GI number: 77457329

Start: 1275987

End: 1276850

Strand: Direct

Name: tsf [H]

Synonym: Pfl01_1102

Alternate gene names: 77457329

Gene position: 1275987-1276850 (Clockwise)

Preceding gene: 77457328

Following gene: 77457330

Centisome position: 19.82

GC content: 55.32

Gene sequence:

>864_bases
ATGGCAGCAATTACTGCAGCGTTGGTCAAAGAACTGCGCGAGCGTACCGGCGAAGGCATGATGGATTGCAAGAAAGCCCT
GGAAAAGGCTGGCGGCGACATCGAAAAAGCCATTGATGACATGCGTGCTTCGGGCGCCATCAAGGCTGCCAAGAAAGCTG
GCAACGTTGCCGCTGAAGGCGCTATCGCCATCAAGGACGACGGCAAGGCTGCCGTTCTGATCGAAGTCAACTCGCAGACC
GACTTCCTGGCTCTGCAAGACGACTTCAAAAACTTCGTTGCTGCCAGCGTTGACAAAGCATTCGCTGACAAGCTGACCGA
CGCAGCTCCGCTGATCGCTGCTCAAGAAGCCGCTCGCGAAGCACTGGTTGCCAAAGTAGGCGAGAACGTCAACATCCGTC
GCCTGGTGCGCGTAGAAGGCGACGTAGTCGGCACCTACCTGCACGGCAACAAGATCGGTGTTGCTGTTGTCCTGAAAGGT
GGCGACGTCGAGCTGGCCAAAGACATCGCAATGCACGTTGCTGCAAGCAACCCTGAGTTCCTGCTGCCTTCGCAAGTCTC
CGACGAAGCGATCGAGCGTGAAAAGGCTGTGTTCCTGCAGCTGAACGAAGAAAAAATCAAAGGCAAGCCAGAAAACATTG
TTGAGAACATGGTCAAGGGCCGTATCAGCAAGTTCCTGGCAGAAGCGAGCCTGGTTGAGCAGGCGTTCGTCAAGAACCCT
GAAATCAAGGTTGGCGAGCTGGCCAAGAAAGGCGGCGCTGAAATCGTTTCCTTCACCTACTTCAAAGTAGGCGAAGGCAT
CGAGAAGCCGGTCGACAACTTCGCTGAAGAAGTTGCTGCTCAGCTGGCTGCCGCCAAGCAATAA

Upstream 100 bases:

>100_bases
GCATCTGTAACGTGCAGCGGCTACAAGGTGGCTCGGGAAGAATTGAACGCCCGTTCGATCGGGTGGAATGGTTGAAAACC
TATCCAAGAGGAATTTTAAA

Downstream 100 bases:

>100_bases
GACGGTTTTTCAACTGTCGCCCGAAAGAGGCTGCCCGCTCACGCGCGCAGCCTCTTTTCAGATAGGGTTACCAATTTTTA
ATTGGTTTCCCCTTGGAACT

Product: elongation factor Ts

Products: NA

Alternate protein names: EF-Ts [H]

Number of amino acids: Translated: 287; Mature: 286

Protein sequence:

>287_residues
MAAITAALVKELRERTGEGMMDCKKALEKAGGDIEKAIDDMRASGAIKAAKKAGNVAAEGAIAIKDDGKAAVLIEVNSQT
DFLALQDDFKNFVAASVDKAFADKLTDAAPLIAAQEAAREALVAKVGENVNIRRLVRVEGDVVGTYLHGNKIGVAVVLKG
GDVELAKDIAMHVAASNPEFLLPSQVSDEAIEREKAVFLQLNEEKIKGKPENIVENMVKGRISKFLAEASLVEQAFVKNP
EIKVGELAKKGGAEIVSFTYFKVGEGIEKPVDNFAEEVAAQLAAAKQ

Sequences:

>Translated_287_residues
MAAITAALVKELRERTGEGMMDCKKALEKAGGDIEKAIDDMRASGAIKAAKKAGNVAAEGAIAIKDDGKAAVLIEVNSQT
DFLALQDDFKNFVAASVDKAFADKLTDAAPLIAAQEAAREALVAKVGENVNIRRLVRVEGDVVGTYLHGNKIGVAVVLKG
GDVELAKDIAMHVAASNPEFLLPSQVSDEAIEREKAVFLQLNEEKIKGKPENIVENMVKGRISKFLAEASLVEQAFVKNP
EIKVGELAKKGGAEIVSFTYFKVGEGIEKPVDNFAEEVAAQLAAAKQ
>Mature_286_residues
AAITAALVKELRERTGEGMMDCKKALEKAGGDIEKAIDDMRASGAIKAAKKAGNVAAEGAIAIKDDGKAAVLIEVNSQTD
FLALQDDFKNFVAASVDKAFADKLTDAAPLIAAQEAAREALVAKVGENVNIRRLVRVEGDVVGTYLHGNKIGVAVVLKGG
DVELAKDIAMHVAASNPEFLLPSQVSDEAIEREKAVFLQLNEEKIKGKPENIVENMVKGRISKFLAEASLVEQAFVKNPE
IKVGELAKKGGAEIVSFTYFKVGEGIEKPVDNFAEEVAAQLAAAKQ

Specific function: Associates with the EF-Tu.GDP complex and induces the exchange of GDP to GTP. It remains bound to the aminoacyl-tRNA.EF- Tu.GTP complex up to the GTP hydrolysis stage on the ribosome [H]

COG id: COG0264

COG function: function code J; Translation elongation factor Ts

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the EF-Ts family [H]

Homologues:

Organism=Homo sapiens, GI171846268, Length=292, Percent_Identity=24.3150684931507, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI291084498, Length=96, Percent_Identity=37.5, Blast_Score=67, Evalue=2e-11,
Organism=Homo sapiens, GI291084502, Length=96, Percent_Identity=37.5, Blast_Score=66, Evalue=3e-11,
Organism=Homo sapiens, GI291084500, Length=96, Percent_Identity=37.5, Blast_Score=66, Evalue=3e-11,
Organism=Escherichia coli, GI1786366, Length=280, Percent_Identity=57.8571428571429, Blast_Score=292, Evalue=2e-80,

Paralogues:

None

Copy number: 2670 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1100 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 4173 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 14563 Molecules/Cell In: Growth Phase, Gluco

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001816
- InterPro:   IPR014039
- InterPro:   IPR018101
- InterPro:   IPR009060
- InterPro:   IPR000449 [H]

Pfam domain/function: PF00889 EF_TS; PF00627 UBA [H]

EC number: NA

Molecular weight: Translated: 30473; Mature: 30342

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS01126 EF_TS_1 ; PS01127 EF_TS_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAITAALVKELRERTGEGMMDCKKALEKAGGDIEKAIDDMRASGAIKAAKKAGNVAAEG
CHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHCCCEECCC
AIAIKDDGKAAVLIEVNSQTDFLALQDDFKNFVAASVDKAFADKLTDAAPLIAAQEAARE
EEEEECCCCEEEEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
ALVAKVGENVNIRRLVRVEGDVVGTYLHGNKIGVAVVLKGGDVELAKDIAMHVAASNPEF
HHHHHHCCCCCEEEEEEECCCEEEEEEECCEEEEEEEEECCCHHHHHHHHHHHCCCCCCE
LLPSQVSDEAIEREKAVFLQLNEEKIKGKPENIVENMVKGRISKFLAEASLVEQAFVKNP
ECCCCCCHHHHHHHHHEEEEECHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
EIKVGELAKKGGAEIVSFTYFKVGEGIEKPVDNFAEEVAAQLAAAKQ
CCCHHHHHHCCCCCEEEEEHHHHCCCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
AAITAALVKELRERTGEGMMDCKKALEKAGGDIEKAIDDMRASGAIKAAKKAGNVAAEG
HHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHCCCEECCC
AIAIKDDGKAAVLIEVNSQTDFLALQDDFKNFVAASVDKAFADKLTDAAPLIAAQEAARE
EEEEECCCCEEEEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
ALVAKVGENVNIRRLVRVEGDVVGTYLHGNKIGVAVVLKGGDVELAKDIAMHVAASNPEF
HHHHHHCCCCCEEEEEEECCCEEEEEEECCEEEEEEEEECCCHHHHHHHHHHHCCCCCCE
LLPSQVSDEAIEREKAVFLQLNEEKIKGKPENIVENMVKGRISKFLAEASLVEQAFVKNP
ECCCCCCHHHHHHHHHEEEEECHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
EIKVGELAKKGGAEIVSFTYFKVGEGIEKPVDNFAEEVAAQLAAAKQ
CCCHHHHHHCCCCCEEEEEHHHHCCCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12928499 [H]