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The map label for this gene is estA [H]

Identifier: 77457199

GI number: 77457199

Start: 1132320

End: 1132976

Strand: Reverse

Name: estA [H]

Synonym: Pfl01_0972

Alternate gene names: 77457199

Gene position: 1132976-1132320 (Counterclockwise)

Preceding gene: 77457209

Following gene: 255961279

Centisome position: 17.6

GC content: 60.58

Gene sequence:

>657_bases
ATGACCGAGCCCTTGATTCTTCAGCCTGTTAAGCCCGCAGACGCCTGCGTGATCTGGCTGCACGGCCTCGGCGCCGACCG
CTACGACTTCCTGCCGGTGGCCGAAGCGCTTCAGGAAAGCCTGCTGAGCACGCGTTTCGTCCTGCCCCAGGCGCCGACCC
GTCCGGTGACGATAAATGGCGGATATGCCATGCCGAGCTGGTACGACATCAAGGCGATGAGCCCGGCCCGGGCGATCGAC
CGCGATGAGCTGGAAGCGTCTGCCGATCGCATCATCGAATTGATCGAAGAACAGCGAAGCAGCGGAATAGACGCCTCGCG
GATTTTCCTCGCCGGTTTTTCCCAGGGCGGCGCCGTGGTCTATCACACCGCATTCCTGAAGTGGCAAGGGCCATTGGGTG
GGGTACTGGCACTATCGACATACGCGCCGACCTTCAGTGATGAACTGGAGTTGTCCGCCAGCCAGCAGCGGATTCCGGTG
CTGGCGCTGCACGGCCAGTTCGACAACGTGGTGCAGAACTCGATGGGACGCACGGCTTATGAGTATCTCAAGGCCAATGG
TGTCACCGTGACATGGCAGGAATACCCAATGGAGCACGAAGTGTTACCCGAAGAAATTCGCGACATCGGCACATGGTTGA
GCGAACGCCTGCGCTGA

Upstream 100 bases:

>100_bases
AGCAAAACTTGCAGCCTTGCGACATCTTCTTAACGGATCAACCGGGCGCGCACGCAAATCACGCACTCAATGAGAGCGCC
CGCACACAATTGGAGCAGCC

Downstream 100 bases:

>100_bases
CAATTGCGACAGGGGTCGCCCTTTGATCATCCCACTACGCCGCGCCCGTTTCTTGCATTACACTGGCCGGCGTACATTCC
TTAACCAATTGATGAGATGA

Product: phospholipase/carboxylesterase

Products: NA

Alternate protein names: Esterase I [H]

Number of amino acids: Translated: 218; Mature: 217

Protein sequence:

>218_residues
MTEPLILQPVKPADACVIWLHGLGADRYDFLPVAEALQESLLSTRFVLPQAPTRPVTINGGYAMPSWYDIKAMSPARAID
RDELEASADRIIELIEEQRSSGIDASRIFLAGFSQGGAVVYHTAFLKWQGPLGGVLALSTYAPTFSDELELSASQQRIPV
LALHGQFDNVVQNSMGRTAYEYLKANGVTVTWQEYPMEHEVLPEEIRDIGTWLSERLR

Sequences:

>Translated_218_residues
MTEPLILQPVKPADACVIWLHGLGADRYDFLPVAEALQESLLSTRFVLPQAPTRPVTINGGYAMPSWYDIKAMSPARAID
RDELEASADRIIELIEEQRSSGIDASRIFLAGFSQGGAVVYHTAFLKWQGPLGGVLALSTYAPTFSDELELSASQQRIPV
LALHGQFDNVVQNSMGRTAYEYLKANGVTVTWQEYPMEHEVLPEEIRDIGTWLSERLR
>Mature_217_residues
TEPLILQPVKPADACVIWLHGLGADRYDFLPVAEALQESLLSTRFVLPQAPTRPVTINGGYAMPSWYDIKAMSPARAIDR
DELEASADRIIELIEEQRSSGIDASRIFLAGFSQGGAVVYHTAFLKWQGPLGGVLALSTYAPTFSDELELSASQQRIPVL
ALHGQFDNVVQNSMGRTAYEYLKANGVTVTWQEYPMEHEVLPEEIRDIGTWLSERLR

Specific function: Hydrolyzes carboxylic ester bonds with relatively broad substrate specificity [H]

COG id: COG0400

COG function: function code R; Predicted esterase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase 2 family [H]

Homologues:

Organism=Homo sapiens, GI5453722, Length=219, Percent_Identity=34.703196347032, Blast_Score=121, Evalue=4e-28,
Organism=Homo sapiens, GI9966764, Length=208, Percent_Identity=34.6153846153846, Blast_Score=111, Evalue=6e-25,
Organism=Homo sapiens, GI20270341, Length=218, Percent_Identity=27.0642201834862, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17508057, Length=221, Percent_Identity=30.316742081448, Blast_Score=105, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6323147, Length=220, Percent_Identity=29.5454545454545, Blast_Score=99, Evalue=7e-22,
Organism=Drosophila melanogaster, GI45553055, Length=216, Percent_Identity=31.0185185185185, Blast_Score=122, Evalue=1e-28,
Organism=Drosophila melanogaster, GI45553057, Length=216, Percent_Identity=31.0185185185185, Blast_Score=122, Evalue=1e-28,
Organism=Drosophila melanogaster, GI21357257, Length=216, Percent_Identity=31.0185185185185, Blast_Score=122, Evalue=1e-28,
Organism=Drosophila melanogaster, GI161078179, Length=213, Percent_Identity=29.1079812206573, Blast_Score=76, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003140 [H]

Pfam domain/function: PF02230 Abhydrolase_2 [H]

EC number: =3.1.1.1 [H]

Molecular weight: Translated: 24235; Mature: 24104

Theoretical pI: Translated: 4.51; Mature: 4.51

Prosite motif: PS00237 G_PROTEIN_RECEP_F1_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEPLILQPVKPADACVIWLHGLGADRYDFLPVAEALQESLLSTRFVLPQAPTRPVTING
CCCCEEEECCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHEEECCCCCCCCEEEEC
GYAMPSWYDIKAMSPARAIDRDELEASADRIIELIEEQRSSGIDASRIFLAGFSQGGAVV
CCCCCCEEEEECCCCHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHEEEEEECCCCCEEE
YHTAFLKWQGPLGGVLALSTYAPTFSDELELSASQQRIPVLALHGQFDNVVQNSMGRTAY
EEEEEEEECCCCCCEEEEEECCCCCCCHHCCCCCCCCCCEEEECCCHHHHHHHHCCHHHH
EYLKANGVTVTWQEYPMEHEVLPEEIRDIGTWLSERLR
HHHHCCCCEEEHHCCCCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TEPLILQPVKPADACVIWLHGLGADRYDFLPVAEALQESLLSTRFVLPQAPTRPVTING
CCCEEEECCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHEEECCCCCCCCEEEEC
GYAMPSWYDIKAMSPARAIDRDELEASADRIIELIEEQRSSGIDASRIFLAGFSQGGAVV
CCCCCCEEEEECCCCHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHEEEEEECCCCCEEE
YHTAFLKWQGPLGGVLALSTYAPTFSDELELSASQQRIPVLALHGQFDNVVQNSMGRTAY
EEEEEEEECCCCCCEEEEEECCCCCCCHHCCCCCCCCCCEEEECCCHHHHHHHHCCHHHH
EYLKANGVTVTWQEYPMEHEVLPEEIRDIGTWLSERLR
HHHHCCCCEEEHHCCCCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA