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The map label for this gene is dppA [H]

Identifier: 77457042

GI number: 77457042

Start: 961253

End: 962851

Strand: Reverse

Name: dppA [H]

Synonym: Pfl01_0815

Alternate gene names: 77457042

Gene position: 962851-961253 (Counterclockwise)

Preceding gene: 77457043

Following gene: 77457041

Centisome position: 14.95

GC content: 57.97

Gene sequence:

>1599_bases
ATGCTTAAACACGCGGTCATTCCGTTTTTAGTCGGCGCAGGCTTGTTAGCCTCCGCACCTTTCGCCACCGCTGCGACTAA
CCTGGTGTTCTGCTCCGAAGGCAGCCCGGCCGGTTTCGATCCAGGCCAGTACACCACCGGAACCGACTTCGACGCCTCAG
CCGAAACCATGTTCAACCGTCTGACCCAGTTCGAGCGTGGCGGTACCGCCGTGATTCCTGGTCTGGCGACCAAGTGGGAC
ATCTCGGATGACGGCCTGACTTACACCTTCCACCTGCGTGAAGGCGTCAAGTTCCACACCACCCCGTATTTCAAGCCGAC
TCGTGAGTTCAACGCCGACGACGTGCTGTTCACCTTCAATCGCATGATTAACAAGGATGACCCGTTCCGTAAGGCGTACC
CGACCGAATTCCCGTACTTCACCGACATGGGGATGGACACCAACATCACCAAGATCGATAAAGTCGACGACCACACCGTC
AAGTTCACTCTGAAAGAAGTCGACGCCGCGTTCATCCAGAACATGGCCATGAGCTTCGCGTCGGTTCAGTCCGCCGAGTA
CGCAGCCCAGCTGCTGAAAGAAGGCAAGGCTGCCGACATCAACCAGAAGCCGATCGGCACTGGCCCGTTCGTGTTCAAGA
GCTATCAGAAAGACTCGAACATCCGTTACACCGGCAACCCGCATTACTGGGATCCGAGCCGGGTGAAGCTGAAGAACCTG
ATTTTTGCGATCAATACCGACGCCTCGGTGCGCGTGCAAAAGCTCAAGGCCGGCGAATGCCAGATCACCCTGCACCCGCG
CCCTGCCGATGTGCCGGCACTGAAGAACGATCCGAAACTGCAATTGATCGAGAAGCCGGGCTTCAACCTTGGCTACATCG
CCTACAACGTCCGTCACAAGCCGTTCGACCAGCTCGAAGTGCGTCAGGCGCTGGATATGGCAGTAAATAAACAGGGGATT
CTCAACGCTGTTTATCAAGGCGCCGGCCAACTGGCCGTCAACGCCATGCCGCCGACCCAATGGTCCTACGACGACACGAT
CAAGGACGCCGCCTACAACCCGGAAAAAGCCAAAGAGCTGCTCAAGGCTGCCGGCGTCAAGGAAGGCACCGAGATCACCT
TATGGGCGATGCCGGTACAACGTCCGTACAACCCGAACGCCAAACTGATGGCCGAGATGCTCCAGGCGGACTGGGCCAAG
ATCGGTCTGAAAGTGAAGATCGTCAGCTACGAATGGGGCGAGTACATCAAGCGCACCAAGAATGGCGAGCACGACATCAG
CCTGATCGGCTGGACCGGTGACAACGGGGACCCGGACAACTGGCTCGGCACGCTGTACAGCTGCGACGCCATCGGCGGCA
ACAACTACTCCATGTGGTGCGATCCGGCTTACGACAAGCTGATCAAACAGGCCAAGGTCGTCACCGACCGTGACCAACGC
ACCGTGCTCTATAAACAGGCCCAGCAACTGCTCAAGCAGCAAGTGCCGATCACGCCTGTCGCCCACTCGACGGTCAACCA
GCCGCTGAGCGCCAAAGTCGAAGGGTTCAAGGTGAGCCCGTTCGGTCGCAACGTGTTTTCGGGTGTCAGCATCGATTAA

Upstream 100 bases:

>100_bases
GGATCTTGCATTGGGTATGGGCCCTGCATAAGTATCCGCAGGCACGACTCACGAGGTCGTACCTCACTACTAAAAAATGA
CAACAAATCATGAGGCCAAC

Downstream 100 bases:

>100_bases
ACAAACAACCGATTAGCCGCAATGCTGAGGGGGCCGTCTACCCCCTCACGCAAGCGCTTTGCCGAAATTCGCCAATCTGG
CCTTTTGCAAACGTTTGCGA

Product: extracellular solute-binding protein

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: Dipeptide-binding protein; DBP [H]

Number of amino acids: Translated: 532; Mature: 532

Protein sequence:

>532_residues
MLKHAVIPFLVGAGLLASAPFATAATNLVFCSEGSPAGFDPGQYTTGTDFDASAETMFNRLTQFERGGTAVIPGLATKWD
ISDDGLTYTFHLREGVKFHTTPYFKPTREFNADDVLFTFNRMINKDDPFRKAYPTEFPYFTDMGMDTNITKIDKVDDHTV
KFTLKEVDAAFIQNMAMSFASVQSAEYAAQLLKEGKAADINQKPIGTGPFVFKSYQKDSNIRYTGNPHYWDPSRVKLKNL
IFAINTDASVRVQKLKAGECQITLHPRPADVPALKNDPKLQLIEKPGFNLGYIAYNVRHKPFDQLEVRQALDMAVNKQGI
LNAVYQGAGQLAVNAMPPTQWSYDDTIKDAAYNPEKAKELLKAAGVKEGTEITLWAMPVQRPYNPNAKLMAEMLQADWAK
IGLKVKIVSYEWGEYIKRTKNGEHDISLIGWTGDNGDPDNWLGTLYSCDAIGGNNYSMWCDPAYDKLIKQAKVVTDRDQR
TVLYKQAQQLLKQQVPITPVAHSTVNQPLSAKVEGFKVSPFGRNVFSGVSID

Sequences:

>Translated_532_residues
MLKHAVIPFLVGAGLLASAPFATAATNLVFCSEGSPAGFDPGQYTTGTDFDASAETMFNRLTQFERGGTAVIPGLATKWD
ISDDGLTYTFHLREGVKFHTTPYFKPTREFNADDVLFTFNRMINKDDPFRKAYPTEFPYFTDMGMDTNITKIDKVDDHTV
KFTLKEVDAAFIQNMAMSFASVQSAEYAAQLLKEGKAADINQKPIGTGPFVFKSYQKDSNIRYTGNPHYWDPSRVKLKNL
IFAINTDASVRVQKLKAGECQITLHPRPADVPALKNDPKLQLIEKPGFNLGYIAYNVRHKPFDQLEVRQALDMAVNKQGI
LNAVYQGAGQLAVNAMPPTQWSYDDTIKDAAYNPEKAKELLKAAGVKEGTEITLWAMPVQRPYNPNAKLMAEMLQADWAK
IGLKVKIVSYEWGEYIKRTKNGEHDISLIGWTGDNGDPDNWLGTLYSCDAIGGNNYSMWCDPAYDKLIKQAKVVTDRDQR
TVLYKQAQQLLKQQVPITPVAHSTVNQPLSAKVEGFKVSPFGRNVFSGVSID
>Mature_532_residues
MLKHAVIPFLVGAGLLASAPFATAATNLVFCSEGSPAGFDPGQYTTGTDFDASAETMFNRLTQFERGGTAVIPGLATKWD
ISDDGLTYTFHLREGVKFHTTPYFKPTREFNADDVLFTFNRMINKDDPFRKAYPTEFPYFTDMGMDTNITKIDKVDDHTV
KFTLKEVDAAFIQNMAMSFASVQSAEYAAQLLKEGKAADINQKPIGTGPFVFKSYQKDSNIRYTGNPHYWDPSRVKLKNL
IFAINTDASVRVQKLKAGECQITLHPRPADVPALKNDPKLQLIEKPGFNLGYIAYNVRHKPFDQLEVRQALDMAVNKQGI
LNAVYQGAGQLAVNAMPPTQWSYDDTIKDAAYNPEKAKELLKAAGVKEGTEITLWAMPVQRPYNPNAKLMAEMLQADWAK
IGLKVKIVSYEWGEYIKRTKNGEHDISLIGWTGDNGDPDNWLGTLYSCDAIGGNNYSMWCDPAYDKLIKQAKVVTDRDQR
TVLYKQAQQLLKQQVPITPVAHSTVNQPLSAKVEGFKVSPFGRNVFSGVSID

Specific function: Dipeptide-binding protein of a transport system that can be subject to osmotic shock. DppA is also required for peptide chemotaxis [H]

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=524, Percent_Identity=51.7175572519084, Blast_Score=576, Evalue=1e-165,
Organism=Escherichia coli, GI1787551, Length=538, Percent_Identity=33.8289962825279, Blast_Score=350, Evalue=1e-97,
Organism=Escherichia coli, GI1787762, Length=534, Percent_Identity=27.3408239700375, Blast_Score=172, Evalue=7e-44,
Organism=Escherichia coli, GI1787052, Length=461, Percent_Identity=27.765726681128, Blast_Score=171, Evalue=9e-44,
Organism=Escherichia coli, GI87081878, Length=525, Percent_Identity=26.2857142857143, Blast_Score=129, Evalue=6e-31,
Organism=Escherichia coli, GI1789397, Length=519, Percent_Identity=24.2774566473988, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI1787495, Length=450, Percent_Identity=25.1111111111111, Blast_Score=119, Evalue=6e-28,
Organism=Escherichia coli, GI1789887, Length=525, Percent_Identity=23.8095238095238, Blast_Score=115, Evalue=5e-27,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 59278; Mature: 59278

Theoretical pI: Translated: 7.62; Mature: 7.62

Prosite motif: PS01040 SBP_BACTERIAL_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKHAVIPFLVGAGLLASAPFATAATNLVFCSEGSPAGFDPGQYTTGTDFDASAETMFNR
CCCHHHHHHHHHHHHHHCCCCHHHHCEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHH
LTQFERGGTAVIPGLATKWDISDDGLTYTFHLREGVKFHTTPYFKPTREFNADDVLFTFN
HHHHHCCCCEEECCCCEEECCCCCCEEEEEEECCCCEEECCCCCCCCCCCCCCCEEEEHH
RMINKDDPFRKAYPTEFPYFTDMGMDTNITKIDKVDDHTVKFTLKEVDAAFIQNMAMSFA
HHCCCCCCCHHCCCCCCCEEECCCCCCCCEEECCCCCCEEEEEHHHHHHHHHHHHHHHHH
SVQSAEYAAQLLKEGKAADINQKPIGTGPFVFKSYQKDSNIRYTGNPHYWDPSRVKLKNL
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHEECCCCCCCEEECCCCCCCCCCCEEEEEE
IFAINTDASVRVQKLKAGECQITLHPRPADVPALKNDPKLQLIEKPGFNLGYIAYNVRHK
EEEECCCCCEEEEEECCCCEEEEECCCCCCCCCCCCCCCEEEEECCCCCEEEEEEECCCC
PFDQLEVRQALDMAVNKQGILNAVYQGAGQLAVNAMPPTQWSYDDTIKDAAYNPEKAKEL
CHHHHHHHHHHHHHHCCHHHHHHHHCCCCCEEEECCCCCCCCCCCHHHHCCCCHHHHHHH
LKAAGVKEGTEITLWAMPVQRPYNPNAKLMAEMLQADWAKIGLKVKIVSYEWGEYIKRTK
HHHCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCEEEEEEEECHHHHHHHCC
NGEHDISLIGWTGDNGDPDNWLGTLYSCDAIGGNNYSMWCDPAYDKLIKQAKVVTDRDQR
CCCCCEEEEEEECCCCCCHHHHHHHEEEECCCCCCCEEEECHHHHHHHHHHHHHCCCCHH
TVLYKQAQQLLKQQVPITPVAHSTVNQPLSAKVEGFKVSPFGRNVFSGVSID
HHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCCCCEEECCCCCHHCCCCCCC
>Mature Secondary Structure
MLKHAVIPFLVGAGLLASAPFATAATNLVFCSEGSPAGFDPGQYTTGTDFDASAETMFNR
CCCHHHHHHHHHHHHHHCCCCHHHHCEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHH
LTQFERGGTAVIPGLATKWDISDDGLTYTFHLREGVKFHTTPYFKPTREFNADDVLFTFN
HHHHHCCCCEEECCCCEEECCCCCCEEEEEEECCCCEEECCCCCCCCCCCCCCCEEEEHH
RMINKDDPFRKAYPTEFPYFTDMGMDTNITKIDKVDDHTVKFTLKEVDAAFIQNMAMSFA
HHCCCCCCCHHCCCCCCCEEECCCCCCCCEEECCCCCCEEEEEHHHHHHHHHHHHHHHHH
SVQSAEYAAQLLKEGKAADINQKPIGTGPFVFKSYQKDSNIRYTGNPHYWDPSRVKLKNL
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHEECCCCCCCEEECCCCCCCCCCCEEEEEE
IFAINTDASVRVQKLKAGECQITLHPRPADVPALKNDPKLQLIEKPGFNLGYIAYNVRHK
EEEECCCCCEEEEEECCCCEEEEECCCCCCCCCCCCCCCEEEEECCCCCEEEEEEECCCC
PFDQLEVRQALDMAVNKQGILNAVYQGAGQLAVNAMPPTQWSYDDTIKDAAYNPEKAKEL
CHHHHHHHHHHHHHHCCHHHHHHHHCCCCCEEEECCCCCCCCCCCHHHHCCCCHHHHHHH
LKAAGVKEGTEITLWAMPVQRPYNPNAKLMAEMLQADWAKIGLKVKIVSYEWGEYIKRTK
HHHCCCCCCCEEEEEEEECCCCCCCCHHHHHHHHHHHHHHCCEEEEEEEECHHHHHHHCC
NGEHDISLIGWTGDNGDPDNWLGTLYSCDAIGGNNYSMWCDPAYDKLIKQAKVVTDRDQR
CCCCCEEEEEEECCCCCCHHHHHHHEEEECCCCCCCEEEECHHHHHHHHHHHHHCCCCHH
TVLYKQAQQLLKQQVPITPVAHSTVNQPLSAKVEGFKVSPFGRNVFSGVSID
HHHHHHHHHHHHHCCCCCCCCHHHCCCCCCCCCCCEEECCCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1702779; 1956284; 7536291; 8041620; 9278503; 9298646; 9600841; 8563629; 8527431 [H]