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The map label for this gene is fruR [H]

Identifier: 77457019

GI number: 77457019

Start: 929500

End: 930495

Strand: Reverse

Name: fruR [H]

Synonym: Pfl01_0792

Alternate gene names: 77457019

Gene position: 930495-929500 (Counterclockwise)

Preceding gene: 77457024

Following gene: 77457018

Centisome position: 14.45

GC content: 63.76

Gene sequence:

>996_bases
TTGAAACTCAGTGATATCGCGCGGTTGGCCGGAGTGTCCGTGACCACCGCCAGCTACGTCATCAACGGCAAGGCCGAACA
GCAACGCATCAGCACCGCCACCGTCGAGCGGGTCCGGGCGGTGGTCGATCTGCACGGTTTTACCCCCAATCCACAGGCGG
CCGGGCTGCGCAGTCGGCACACCCGGACCCTGGGTTTTATCCTGCCGGATCTGGAAAACCCCAGTTACGCGCGAATCGCC
AAACTGCTGGAGCAAGGTGCCCGTGCGCGGGGTTATCAGTTGCTGATCGCCAGCTCCGACGATGCGCCGGACAGCGAACG
GCAGCTGCTGCAACTGTTCCGCGCCCGGCGCTGCGATGCGTTGATCGTCGCCAGTTGCCTGCCGGCCGGTGACGACAGTT
ATCGCCAGTTGCAGACCAAGGGCCTGCCGATCATTGCCATCGACCGGGTGATGGATGCCGAACATTTCTGCTCGGTGGTC
AGCGATGACCGCGAAGCCAGCCTGCACCTGACCGAAAGCCTGCTCGACCCGCAGCCGAAACAGATCGTGCTGCTGGGCGC
CCGCCCGGAACTGAGCATCAGCCAGGAACGTGCCGCCGGTTTCAAACAAGCCCTGGCCGGGTTCAAGGGCGAAGTGCTGA
TCGAACACGCCGAGTCCTTCAGCCGCGAGTGCGGCAAGCAGTTGATGGAAGAACTCCTGCAACGCCTCGGGCATTTGCCG
GATGCGCTGGTGACCACGTCCTACGTACTGTTGCAGGGGGTGTTCGACGCGCTGCATGACTTCCCGCTCAAATCCCGCCC
GCTGCGCCTCGGCACATTCGGCGACACCCAGTTGCTCGACTTCCTGCCGCTGCCGGTCAACGCCATGGCCCAGCAGCACC
AGTTGATCGCCGACAAGGCGCTGGAACTGGCGTTGGCGGCGGTCGAACAATCGGACTACCAGCCTGGCGTGCAGGCCATT
GCCCGGACCTTCAAGCAGCGTATTCACCGGGACTGA

Upstream 100 bases:

>100_bases
TCCAACTTGTCGGATGCGCTGAGACAATGGTCGCCTGCCTGAATCGGGTAGGATTGGCGAAAATGTCGCGGCAAGCTCGA
AAAAACAAGGAAATCCCGGG

Downstream 100 bases:

>100_bases
GCCGTGGAGCTGATCGACAGCCACACCCATCTGGACTTTCCCGACTTCGACGCCGACCGCGCGGCGCTGCTGAGCGAAAG
CCGCGCCCTCGGGGTGCGGC

Product: DNA-binding transcriptional regulator FruR

Products: NA

Alternate protein names: Catabolite repressor/activator [H]

Number of amino acids: Translated: 331; Mature: 331

Protein sequence:

>331_residues
MKLSDIARLAGVSVTTASYVINGKAEQQRISTATVERVRAVVDLHGFTPNPQAAGLRSRHTRTLGFILPDLENPSYARIA
KLLEQGARARGYQLLIASSDDAPDSERQLLQLFRARRCDALIVASCLPAGDDSYRQLQTKGLPIIAIDRVMDAEHFCSVV
SDDREASLHLTESLLDPQPKQIVLLGARPELSISQERAAGFKQALAGFKGEVLIEHAESFSRECGKQLMEELLQRLGHLP
DALVTTSYVLLQGVFDALHDFPLKSRPLRLGTFGDTQLLDFLPLPVNAMAQQHQLIADKALELALAAVEQSDYQPGVQAI
ARTFKQRIHRD

Sequences:

>Translated_331_residues
MKLSDIARLAGVSVTTASYVINGKAEQQRISTATVERVRAVVDLHGFTPNPQAAGLRSRHTRTLGFILPDLENPSYARIA
KLLEQGARARGYQLLIASSDDAPDSERQLLQLFRARRCDALIVASCLPAGDDSYRQLQTKGLPIIAIDRVMDAEHFCSVV
SDDREASLHLTESLLDPQPKQIVLLGARPELSISQERAAGFKQALAGFKGEVLIEHAESFSRECGKQLMEELLQRLGHLP
DALVTTSYVLLQGVFDALHDFPLKSRPLRLGTFGDTQLLDFLPLPVNAMAQQHQLIADKALELALAAVEQSDYQPGVQAI
ARTFKQRIHRD
>Mature_331_residues
MKLSDIARLAGVSVTTASYVINGKAEQQRISTATVERVRAVVDLHGFTPNPQAAGLRSRHTRTLGFILPDLENPSYARIA
KLLEQGARARGYQLLIASSDDAPDSERQLLQLFRARRCDALIVASCLPAGDDSYRQLQTKGLPIIAIDRVMDAEHFCSVV
SDDREASLHLTESLLDPQPKQIVLLGARPELSISQERAAGFKQALAGFKGEVLIEHAESFSRECGKQLMEELLQRLGHLP
DALVTTSYVLLQGVFDALHDFPLKSRPLRLGTFGDTQLLDFLPLPVNAMAQQHQLIADKALELALAAVEQSDYQPGVQAI
ARTFKQRIHRD

Specific function: Represses some operons but activates others. It is implicated in the regulation of a large number of operons encoding enzymes which comprise central pathways of carbon metabolism. Binds D-fructose as an inducer [H]

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786268, Length=330, Percent_Identity=47.8787878787879, Blast_Score=276, Evalue=1e-75,
Organism=Escherichia coli, GI1787906, Length=263, Percent_Identity=30.0380228136882, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI1789068, Length=264, Percent_Identity=29.9242424242424, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1789202, Length=238, Percent_Identity=31.0924369747899, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1790194, Length=319, Percent_Identity=23.8244514106583, Blast_Score=75, Evalue=6e-15,
Organism=Escherichia coli, GI1787948, Length=277, Percent_Identity=24.9097472924188, Blast_Score=75, Evalue=7e-15,
Organism=Escherichia coli, GI1787580, Length=301, Percent_Identity=25.2491694352159, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI48994940, Length=226, Percent_Identity=26.5486725663717, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1790369, Length=307, Percent_Identity=24.7557003257329, Blast_Score=71, Evalue=1e-13,
Organism=Escherichia coli, GI1788474, Length=205, Percent_Identity=27.3170731707317, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1790689, Length=283, Percent_Identity=24.0282685512367, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1790715, Length=206, Percent_Identity=23.7864077669903, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012781
- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36476; Mature: 36476

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLSDIARLAGVSVTTASYVINGKAEQQRISTATVERVRAVVDLHGFTPNPQAAGLRSRH
CCHHHHHHHHCCEEEHHHHEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHC
TRTLGFILPDLENPSYARIAKLLEQGARARGYQLLIASSDDAPDSERQLLQLFRARRCDA
CHHHEEEECCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCH
LIVASCLPAGDDSYRQLQTKGLPIIAIDRVMDAEHFCSVVSDDREASLHLTESLLDPQPK
HHHHHHCCCCCHHHHHHHHCCCCEEEHHHHHCHHHHHHHHCCCCHHHHHHHHHHCCCCCC
QIVLLGARPELSISQERAAGFKQALAGFKGEVLIEHAESFSRECGKQLMEELLQRLGHLP
EEEEEECCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DALVTTSYVLLQGVFDALHDFPLKSRPLRLGTFGDTQLLDFLPLPVNAMAQQHQLIADKA
HHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCHHHHHHCCCCHHHHHHHHHHHHHHH
LELALAAVEQSDYQPGVQAIARTFKQRIHRD
HHHHHHHHHHCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKLSDIARLAGVSVTTASYVINGKAEQQRISTATVERVRAVVDLHGFTPNPQAAGLRSRH
CCHHHHHHHHCCEEEHHHHEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHC
TRTLGFILPDLENPSYARIAKLLEQGARARGYQLLIASSDDAPDSERQLLQLFRARRCDA
CHHHEEEECCCCCCCHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCH
LIVASCLPAGDDSYRQLQTKGLPIIAIDRVMDAEHFCSVVSDDREASLHLTESLLDPQPK
HHHHHHCCCCCHHHHHHHHCCCCEEEHHHHHCHHHHHHHHCCCCHHHHHHHHHHCCCCCC
QIVLLGARPELSISQERAAGFKQALAGFKGEVLIEHAESFSRECGKQLMEELLQRLGHLP
EEEEEECCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DALVTTSYVLLQGVFDALHDFPLKSRPLRLGTFGDTQLLDFLPLPVNAMAQQHQLIADKA
HHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCHHHHHHCCCCHHHHHHHHHHHHHHH
LELALAAVEQSDYQPGVQAIARTFKQRIHRD
HHHHHHHHHHCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]